| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,251,690 | A→C | 22.6% | intergenic (+38/+372) | thiH → / ← htrC | thiamin biosynthesis ThiGH complex subunit/heat shock protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,251,690 | 0 | A | C | 22.6% | 137.7 / 42.1 | 93 | intergenic (+38/+372) | thiH/htrC | thiamin biosynthesis ThiGH complex subunit/heat shock protein |
| Reads supporting (aligned to +/- strand): ref base A (44/28); new base C (9/12); total (53/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.10e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGCCTCGCAAAGACTATGAGACGGAATGTAAGAACGTAAATTTATTCCGAGCCGCTACGGAAGATGAAAAACGCAAGGTTGTTGAAAGCGTTGTGTTTTTTTATGGTAGTGCCGTCAGCAGG > minE/2251634‑2251755 | cgcCTCGCAAAGACTATGAGACGGAATGTAAGAACGTAAATTTATTCCGAGCCGCTACGGAAGATGaaa > 1:2945229/1‑69 (MQ=255)cgcCTCGCAAAGACTATGAGACGGAATGTAAGAACGTAAATTTATTCCGAGCCGCTACGGAAGATGaaa > 1:3380532/1‑69 (MQ=255)cgcCTCGCAAAGACTATGAGACGGAATGTAAGAACGTAAATTTATTCCGAGCCGCTACGGAAGATGaaa > 1:173149/1‑69 (MQ=255)cgcCTCGCAAAGACTATGAGACGGAATGTAAGAACGTAAATTTATTCCGAGCCGCTACGGAAGATGaaa > 1:308216/1‑69 (MQ=255)cgcCTCGCAAAGACTATGAGACGGAATGTAAGAACGTAAATTTATTCCGAGCCGCTACGGAAGATGaaa > 1:1344070/1‑69 (MQ=255)cgcCTCGCAAAGACTATGAGACGGAATGTAAGAACGTAAATTTATTCCGAGCCGCTACGGAAGATGaaa > 1:207015/1‑69 (MQ=255)cgcCTCGCAAAGACTATGAGACGGAATGTAAGAACGTAAATTTATTCCGAGCCGCTACGGAAGATGaaa > 1:825059/1‑69 (MQ=255)cgcCTCGCAAAGACTATGAGACGGAATGTAAGAACGTAAATTTATTCCGAGCCGCTACGGAAGATGaaa > 1:594730/1‑69 (MQ=255) ccTAGCAAAGACTATGAGACGGAATGTAAGAACGTAAATTTATTCCGAGCCGCTACGGAAGATGaaaaa < 1:3315495/69‑1 (MQ=255) cTCGCAAAGACTATGAGACGGAATGTAAGAACGTAAATTTATTCCGAGCCGCTACGGAAGATGaaaaa < 1:2522185/68‑1 (MQ=255) tCGCAAAGGCCATGAGACAGATTGTAAGAACGTAAATTTATTCCGAGCAGCTCCGGAAGATGAAAAAc > 1:109259/1‑68 (MQ=255) tCGCAAAGGCCATGAGACAGAATGTAAGAACGTAAATTTATTCCGAGCAGCTCCGGAAGATGaaaaa > 1:1633103/1‑67 (MQ=255) tCGCAAAGGCCATGAGACAGAATGTAAGAACGTAAATTTATTCCGAGCAGCTCCGGAAGATGAAAAAc > 1:664937/1‑68 (MQ=255) tCGCAAAGGCCATGAGACAGAATGTAAGAACGTAAATTTATTCCGAGCAGCTCCGGAAGATGAAAAAc > 1:2000144/1‑68 (MQ=255) tCGCAAAGGCCATGAGACAGAATGTAAGAACGTAAATTTATTCCGAGCAGCTCCGGAAGATGAAAAAc > 1:771131/1‑68 (MQ=255) tCGCAAAGGCCATGAGACAGAATGTAAGAACGTAAATTTATTCCGAGCAGCTCCGGAAGATGAAAAAc > 1:1179340/1‑68 (MQ=255) tCGCAAAGGCCATGAGACAGAATGTAAGAACGTAAATTTATTCCGAGCAGCTCCGGAAGATGAAAAAc > 1:115722/1‑68 (MQ=255) tCGCAAAGGCCATGAGACAGAATGTAAGAACGTAAATTTATTCCGAGCAGCTCCGGAAGATGAAAAAc > 1:2677497/1‑68 (MQ=255) gcGCAAAGGCCATGAGACAGAATGTAAGAACGTAAATTTATTCCGAGCAGCTCCGGAAGATGAAAAAc > 1:3118939/2‑68 (MQ=255) cGCAAAGACTATGAGACGGAATGTAAGAACGTAAATTTATTCCGAGCCGCTACGGAAGATGAAAAAc > 1:3134089/1‑67 (MQ=255) aaGACTATGAGACGGAATGTAAGAACGTAAATTTATTCCGAGCCGCTACTGAAGATGAAAAACGCAAgg > 1:97365/1‑69 (MQ=255) aaGACTATGAGACGGAATGTAAGAACGTAAATTTATTCCGAGCCGCTACGGAAGATGAAAAACGCAAgg > 1:102148/1‑69 (MQ=255) aaGACTATGAGACGGAATGTAAGAACGTAAATTTATTCCGAGCCGCTACGGAAGATGAAAAACGCAAgg > 1:1871742/1‑69 (MQ=255) aaGACTATGAGACGGAATGTAAGAACGTAAATTTATTCCGAGCCGCTACGGAAGATGAAAAACGCAAgg > 1:1698339/1‑69 (MQ=255) aaGACTATGAGACGGAATGTAAGAACGTAAATTTATTCCGAGCCGCTACGGAAGATGAAAAACGCAAgg > 1:3069304/1‑69 (MQ=255) aaGACTATGAGACGGAATGTAAGAACGTAAATTTATTCCGAGCCGCTACGGAAGATGAAAAACGCAAgg > 1:1641012/1‑69 (MQ=255) aaGACTATGAGACGGAATGTAAGAACGTAAATTTATTCCGAGCCGCTACGGAAGATGAAAAACGCAAgg > 1:3349693/1‑69 (MQ=255) aaGACTATGAGACGGAATGTAAGAACGTAAATTTATTCCGAGCCGCTACGGAAGATGAAAAACGCAAgg > 1:355004/1‑69 (MQ=255) aaGACTATGAGACGGAATGTAAGAACGTAAATTTATTCCGAGCCGCTACGGAAGATGAAAAACGCAAgg > 1:1023693/1‑69 (MQ=255) aaGACTATGAGACGGAATGTAAGAACGTAAATTTATTCCGAGCCGCTACGGAAGATGAAAAACGCAAgg > 1:579835/1‑69 (MQ=255) aaGACTATGAGACGGAATGTAAGAACGTAAATTTATTCCGAGCCGCTACGGAAGATGAAAAACGCAAgg > 1:1305405/1‑69 (MQ=255) aaGACTATGAGACGGAATGTAAGAACGTAAATTTATTCCGAGCCGCTACGGAAGATGAAAAACGCAAgg > 1:887197/1‑69 (MQ=255) aaGACTATGAGACGGAATGTAAGAACGTAAATTTATTCCGAGCCGCTACGGAAGATGAAAAACGCAAg > 1:3067248/1‑68 (MQ=255) aaGACTATGAGACGGAATGTAAGAACGTAAATTTATTCCGAGCCGCTACGGAAGATGAAAAACGCAAg > 1:106152/1‑68 (MQ=255) aaGACTATGAGACGGAATGTAAGAACGTAAATTTATTCCGAGCCGCTACGGAAGATGAAAAACGCAAg > 1:303876/1‑68 (MQ=255) gACTATGAGACGGAATGTAAGAACGTAAATTTATTCCGAGCCGCTACGGAAGATGAAAAACGCAAg > 1:1008803/1‑66 (MQ=255) aCTATGAGACGGAATGTAAGAACGTAAATTTATTCCGAGCCGCTACGGAAGATGAAAAACGCAAg > 1:279425/1‑65 (MQ=255) aCTATGAGACGGAATGTAAGAACGTAAATTTATTCCGAGCCGCTACGGAAGATGAAAAACGCAAGgttg > 1:275504/1‑69 (MQ=255) tATGAGACGGAATGTAAGAACGTAAATTTATTCCGAGCCGCTACGGa > 1:3229292/1‑47 (MQ=255) tATGAGACGGAATGTAAGAACGTAAATTTATTCCGAGCCGCTACGGa < 1:952811/47‑1 (MQ=255) tATGAGACGGAATGTAAGAACGTAAATTTATTCCGAGCCGCTACGGa > 1:2997378/1‑47 (MQ=255) tATGAGACGGAATGTAAGAACGTAAATTTATTCCGAGCCGCTACGGa < 1:3111556/47‑1 (MQ=255) tATGAGACGGAATGTAAGAACGTAAATTTATTCCGAGCCGCTACGGa > 1:2924156/1‑47 (MQ=255) tATGAGACGGAATGTAAGAACGTAAATTTATTCCGAGCCGCTACGGa < 1:993033/47‑1 (MQ=255) tATGAGACGGAATGTAAGAACGTAAATTTATTCCGAGCCGCTACGGa < 1:1101626/47‑1 (MQ=255) tATGAGACGGAATGTAAGAACGTAAATTTATTCCGAGCCGCTACGGa < 1:260070/47‑1 (MQ=255) tATGAGACGGAATGTAAGAACGTAAATTTATTCCGAGCCGCTACGGa < 1:1494157/47‑1 (MQ=255) tATGAGACGGAATGTAAGAACGTAAATTTATTCCGAGCCGCTACGGa < 1:2649076/47‑1 (MQ=255) tATGAGACGGAATGTAAGAACGTAAATTTATTCCGAGCCGCTACGGa > 1:1566909/1‑47 (MQ=255) tATGAGACGGAATGTAAGAACGTAAATTTATTCCGAGCCGCTACGGa > 1:1281199/1‑47 (MQ=255) tGAGACGGAATGTAAGAACGTAAATTTATTCCGAGCCGCTACGGAAGATGAAAAACGCAAGgttgtt > 1:147443/1‑67 (MQ=255) tAAGAACGTAAATTTATTCCGAGCCGCTACGGAAGATGAAAAACGCAAGGTTGTTGAAAGCGTTGTGtt > 1:1168182/1‑69 (MQ=255) tAAGAACGTAAATTTATTCCGAGCCGCTACGGAAGATGAAAAACGCAAGGTTGTTGAAAGCGTTGTGtt > 1:2886021/1‑69 (MQ=255) tAAGAACGTAAATTTATTCCGAGCCGCTACGGAAGATGAAAAACGCAAGGTTGTTGAAAGCGTTGTGtt > 1:1489682/1‑69 (MQ=255) tAAGAACGTAAATTTATTCCGAGCCGCTACGGAAGATGAAAAACGCAAGGTTGTTGAAAGCGTTGTGtt > 1:3272474/1‑69 (MQ=255) aGAACGTAAATTTATTCCGAGCCGCTACGGAAGATGAAAAACGCAAGGTTGTTGAAAGCGTTGTGtttt > 1:309683/1‑69 (MQ=255) aGAACGTAAATTTATTCCGAGCAGCTCCGGAAGATGAAAAACGCAAGGTTGTTGAAAGCGTTGTGttt < 1:3052906/68‑1 (MQ=255) aGAACGTAAATTTATTCCGAGCAGCTCCGGAAGATGAAAAACGCAAGGTTGTTGAAAGCGTTGTGttt < 1:361780/68‑1 (MQ=255) aGAACGTAAATTTATTCCGAGCAGCTCCGGAAGATGAAAAACGCAAGGTTGTTGAAAGCGTTGTGttt < 1:146960/68‑1 (MQ=255) aGAACGTAAATTTATTCCGAGCAGCTCCGGAAGATGAAAAACGCAAGGTTGTTGAAAGCGTTGTGttt < 1:1185562/68‑1 (MQ=255) aGAACGTAAATTTATTCCGAGCAGCTCCGGAAGATGAAAAACGCAAGGTTGTTGAAAGCGTTGTGttt < 1:2581526/68‑1 (MQ=255) aGAACGTAAATTTATTCCGAGCAGCTCCGGAAGATGAAAAACGCAAGGTTGTTGAAAGCGTTGTGttt < 1:2981012/68‑1 (MQ=255) aGAACGTAAATTTATTCCGAGCAGCTCCGGAAGATGAAAAACGCAAGGTTGTTGAAAGCGTTGTGttt < 1:1195535/68‑1 (MQ=255) aGAACGTAAATTTATTCCGAGCAGCTCCGGAAGATGAAAAACGCAAGGTTGTTGAAAGCGTTGTGttt < 1:3143088/68‑1 (MQ=255) aGAACGTAAATTTATTCCGAGCAGCTCCGGAAGATGAAAAACGCAAGGTTGTTGAAAGCGTTGTGttt < 1:1199368/68‑1 (MQ=255) aGAACGTAAATTTATTCCGAGCAGCTCCGGAAGATGAAAAACGCAAGGTTGTTGAAAGCGTTGTGttt < 1:1569576/68‑1 (MQ=255) aGAACGTAAATTTATTCCGAGCAGCTCCGGAAGATGAAAAACGCAAGGTTGTTGAAAGCGTTGTGttt < 1:1408594/68‑1 (MQ=255) aGAACGTAAATTTATTCCGAGCAGCTCCGGAAGATGAAAAACGCAAGGTTGTTGAAAGCGTTGTGttt < 1:2249298/68‑1 (MQ=255) aaCGTAAATTTATTCCGAGCCGCTACTGAAGATGAAAAACGCAAGGTTGTTGAAAGCGTTGTGtttttt < 1:1310989/69‑1 (MQ=255) aaCGTAAATTTATTCCGAGCCGCTACGGAAGATGAAAAACGCAAGGTTGTTGAAAGCGTTGTGtttttt < 1:3148762/69‑1 (MQ=255) aaCGTAAATTTATTCCGAGCCGCTACGGAAGATGAAAAACGCAAGGTTGTTGAAAGCGTTGTGtttttt < 1:3111214/69‑1 (MQ=255) aaCGTAAATTTATTCCGAGCCGCTACGGAAGATGAAAAACGCAAGGTTGTTGAAAGCGTTGTGtttttt < 1:3202286/69‑1 (MQ=255) aaCGTAAATTTATTCCGAGCCGCTACGGAAGATGAAAAACGCAAGGTTGTTGAAAGCGTTGTGtttttt < 1:594184/69‑1 (MQ=255) aaCGTAAATTTATTCCGAGCCGCTACGGAAGATGAAAAACGCAAGGTTGTTGAAAGCGTTGTGtttttt < 1:1489724/69‑1 (MQ=255) aaCGTAAATTTATTCCGAGCCGCTACGGAAGATGAAAAACGCAAGGTTGTTGAAAGCGTTGTGtttttt < 1:3377261/69‑1 (MQ=255) aaCGTAAATTTATTCCGAGCCGCTACGGAAGATGAAAAACGCAAGGTTGTTGAAAGCGTTGTGtttttt < 1:2912077/69‑1 (MQ=255) aCGTAAATTTATTCCGAGCCGCTACGGAAGATGAAAAACGCAAGGTTGTTGAAAGCGTTGTGtttttt > 1:3262807/1‑68 (MQ=255) aCGTAAATTTATTCCGAGCCGCTACGGAAGATGAAAAACGCAAGGTTGTTGAAAGCGTTGTGtttttt > 1:3008918/1‑68 (MQ=255) aaTTTATTCCGAGCCGCTACGGAAGATGAAAAACGCAAGGTTGTTGAAAGc > 1:3149951/1‑51 (MQ=255) aaTTTATTCCGAGCCGCTACGGAAGATGAAAAACGCAAGGTTGTTGAAAGc > 1:486349/1‑51 (MQ=255) aaTTTATTCCGAGCCGCTACGGAAGATGAAAAACGCAAGGTTGTTGAAAGc > 1:1810042/1‑51 (MQ=255) aaTTTATTCCGAGCCGCTACGGAAGATGAAAAACGCAAGGTTGTTGAAAGc > 1:2938468/1‑51 (MQ=255) aaTTTATTCCGAGCCGCTACGGAAGATGAAAAACGCAAGGTTGTTGAAAGCGTTGTGTTTTTTTATGGt < 1:2109882/69‑1 (MQ=255) aaTTTATTCCGAGCCGCTACGGAAGATGAAAAACGCAAGGTTGTTGAAAGCGTTGTGTTTTTTTATGGt < 1:2318255/69‑1 (MQ=255) aaTTTATTCCGAGCCGCTACGGAAGATGAAAAACGCAAGGTTGTTGAAAGCGTTGTGTTTTTTTATGGt < 1:639937/69‑1 (MQ=255) aaTTTATTCCGAGCCGCTACGGAAGATGAAAAACGCAAGGTTGTTGAAAGCGTTGTGTTTTTTTATGGt < 1:2391922/69‑1 (MQ=255) aaTTTATTCCGAGCCGCTACGGAAGATGAAAAACGCAAGGTTGTTGAAAGCGTTGTGTTTTTTTATGGt < 1:941997/69‑1 (MQ=255) aaTTTATTCCGAGCCGCTACGGAAGATGAAAAACGCAAGGTTGTTGAAAGCGTTGTGTTTTTTTATGGt < 1:2875255/69‑1 (MQ=255) aaTTTATTCCGAGCCGCTACGGAAGATGAAAAACGCAAGGTTGTTGAAAGCGTTGTGTTTTTTTATGGt < 1:1119613/69‑1 (MQ=255) aaTTTATTCCGAGCCGCTACGGAAGATGAAAAACGCAAGGTTGTTGAAAGCGTTGTGTTTTTTTATGGt < 1:2633494/69‑1 (MQ=255) aTTTATTCCGAGCCGCTACGGAAGATGAAAAACGCAAGGTTGTTGAAAGCGTTGTGTTTTTTTATGGt < 1:3002203/68‑1 (MQ=255) gCTACGGAAGATGAAAAACGCAAGGTTGTTGAAAGCGTTGTGTTTTTTTATGGTAGTGCCGTCAGCAgg < 1:582770/69‑1 (MQ=255) gCTACGGAAGATGAAAAACGCAAGGTTGTTGAAAGCGTTGTGTTTTTTTATGGTAGTGCCGTCAGCAgg < 1:2554401/69‑1 (MQ=255) | CGCCTCGCAAAGACTATGAGACGGAATGTAAGAACGTAAATTTATTCCGAGCCGCTACGGAAGATGAAAAACGCAAGGTTGTTGAAAGCGTTGTGTTTTTTTATGGTAGTGCCGTCAGCAGG > minE/2251634‑2251755 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |