| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,253,207 | G→A | 43.2% | G1277G (GGC→GGT) | rpoC ← | RNA polymerase, beta prime subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,253,207 | 0 | G | A | 43.2% | 30.7 / 73.1 | 97 | G1277G (GGC→GGT) | rpoC | RNA polymerase, beta prime subunit |
| Reads supporting (aligned to +/- strand): ref base G (34/21); new base A (23/19); total (57/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.36e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01 | |||||||||||
CCCACTTTGCCGTTCGCTTCCAGTTCGCGGTTTGCGATCTTGACGCGAGAGTATTCAACCTGTTCGCCTTCCAGGAAGTCGGAGCTACCCGCGTTAACGATGGTAGCTTTACGCAGCATCTGACGAACGATAA > minE/2253142‑2253274 | cccACTTTGCCGTTCGCTTCCAGTTCGCGGTTTGCGATCTTGACGCGAGAGTATTCAACCTGTTCAcc > 1:1661706/1‑68 (MQ=255)cccACTTTGCCGTTCGCTTCCAGTTCGCGGTTTGCGATCTTGACGCGAGAGTATTCAACCTGTTCACCt > 1:2110956/1‑69 (MQ=255)cccACTTTGCCGTTCGCTTCCAGTTCGCGGTTTGCGATCTTGACGCGAGAGTATTCAACCTGTTCACCt > 1:3295480/1‑69 (MQ=255)cccACTTTGCCGTTCGCTTCCAGTTCGCGGTTTGCGATCTTGACGCGAGAGTATTCAACCTGTTCACCt > 1:101982/1‑69 (MQ=255)cccACTTTGCCGTTCGCTTCCAGTTCGCGGTTTGCGATCTTGACGCGAGAGTATTCAACCTGTTCACCt > 1:1698477/1‑69 (MQ=255)cccACTTTGCCGTTCGCTTCCAGTTCGCGGTTTGCGATCTTGACGCGAGAGTATTCAACCTGTTCACCt > 1:268040/1‑69 (MQ=255)cccACTTTGCCGTTCGCTTCCAGTTCGCGGTTTGCGATCTTGACGCGAGAGTATTCAACCTGTTCACCt > 1:361091/1‑69 (MQ=255)cccACTTTGCCGTTCGCTTCCAGTTCGCGGTTTGCGATCTTGACGCGAGAGTATTCAACCTGTTCACCt > 1:265646/1‑69 (MQ=255)cccACTTTGCCGTTCGCTTCCAGTTCGCGGTTTGCGATCTTGACGCGAGAGTATTCAACCTGTTCACCt > 1:2609436/1‑69 (MQ=255)cccACTTTGCCGTTCGCTTCCAGTTCGCGGTTTGCGATCTTGACGCGAGAGTATTCAACCTGTTCACCt > 1:504073/1‑69 (MQ=255)cccACTTTGCCGTTCGCTTCCAGTTCGCGGTTTGCGATCTTGACGCGAGAGTATTCAACCTGTTCACCt > 1:2429089/1‑69 (MQ=255)cccACTTTGCCGTTCGCTTCCAGTTCGCGGTTTGCGATCTTGACGCGAGAGTATTCAACCTGTTCACCt > 1:690623/1‑69 (MQ=255)cccACTTTGCCGTTCGCTTCCAGTTCGCGGTTTGCGATCTTGACGCGAGAGTATTCAACCTGTTCACCt > 1:850938/1‑69 (MQ=255)cccACTTTGCCGTTCGCTTCCAGTTCGCGGTTTGCGATCTTGACGCGAGAGTATTCAACCTGTTCACCt > 1:2327750/1‑69 (MQ=255)cccACTTTGCCGTTCGCTTCCAGTTCGCGGTTTGCGATCTTGACGCGAGAGTATTCAACCAGTTCACCt > 1:78556/1‑69 (MQ=255) cGCTTCCAGTTCGCGGTTTGCGATCTTGACGCGAGAGTATTCAACCTGTTCGCCTTCCAGGAAGTCGGa > 1:979829/1‑69 (MQ=255) cGCTTCCAGTTCGCGGTTTGCGATCTTGACGCGAGAGTATTCAACCTGTTCGCCTTCCAGGAAGTCGGa > 1:2239652/1‑69 (MQ=255) cGCTTCCAGTTCGCGGTTTGCGATCTTGACGCGAGAGTATTCAACCTGTTCGCCTTCCAGGAAGTCGGa > 1:3317312/1‑69 (MQ=255) cGCTTCCAGTTCGCGGTTTGCGATCTTGACGCGAGAGTATTCAACCTGTTCGCCTTCCAGGAAGTCGGa > 1:1746809/1‑69 (MQ=255) cGCTTCCAGTTCGCGGTTTGCGATCTTGACGCGAGAGTATTCAACCTGTTCGCCTTCCAGGAAGTCGGa > 1:495876/1‑69 (MQ=255) tCGCGGTTTGCGATCTTGACGCGAGAGTATTCAACCTGTTCGCCTTCCAGGAAGTCGGAGCTACCcgc > 1:2374537/1‑68 (MQ=255) cgcgGTTTGCGATCTTGACGCGAGAGTATTCAACCTGTTCGCCTTCCAGGAAGTCGGAGCTACCcgcg > 1:1688756/1‑68 (MQ=255) cgcgGTTTGCGATCTTGACGCGAGAGTATTCAACCTGTTCGCCTTCCAGGAAGTCGGAGCTACCcgcg > 1:488905/1‑68 (MQ=255) cgcgGTTTGCGATCTTGACGCGAGAGTATTCAACCTGTTCGCCTTCCAGGAAGTCGGAGCTACCcgcg > 1:1407522/1‑68 (MQ=255) cgcgGTTTGCGATCTTGACGCGAGAGTATTCAACCTGTTCGCCTTCCAGGAAGTCGGAGCTACCcgcg > 1:1153714/1‑68 (MQ=255) cgcgGTTTGCGATCTTGACGCGAGAGTATTCAACCTGTTCGCCTTCCAGGAAGTCGGAGCTACCcgcg > 1:560290/1‑68 (MQ=255) cgcgGTTTGCGATCTTGACGCGAGAGTATTCAACCTGTTCGCCTTCCAGGAAGTCGGAGCTACCcgcg > 1:2334326/1‑68 (MQ=255) cgcgGTTTGCGATCTTGACGCGAGAGTATTCAACCTGTTCGCATTCCAGGAAGTCGGAGCTACCcgcg > 1:2434128/1‑68 (MQ=255) tGCGATCTTGACGCGAGAGTATTCAACCTGTTCGCCTTCCAGGAAGTCGGAGTTACCCGCGTTAACGAt < 1:3386453/69‑1 (MQ=255) tGCGATCTTGACGCGAGAGTATTCAACCTGTTCGCCTTCCAGGAAGTCGGAGTTACCCGCGTTAACGAt < 1:2700901/69‑1 (MQ=255) tGCGATCTTGACGCGAGAGTATTCAACCTGTTCGCCTTCCAGGAAGTCGGAGTTACCCGCGTTAACGAt < 1:2516896/69‑1 (MQ=255) tGCGATCTTGACGCGAGAGTATTCAACCTGTTCGCCTTCCAGGAAGTCGGAGTTACCCGCGTTAACGAt < 1:2481607/69‑1 (MQ=255) tGCGATCTTGACGCGAGAGTATTCAACCTGTTCGCCTTCCAGGAAGTCGGAGTTACCCGCGTTAACGAt < 1:27303/69‑1 (MQ=255) tGCGATCTTGACGCGAGAGTATTCAACCTGTTCGCCTTCCAGGAAGTCGGAGTTACCCGCGTTAACGAt < 1:2334117/69‑1 (MQ=255) tGCGATCTTGACGCGAGAGTATTCAACCTGTTCGCCTTCCAGGAAGTCGGAGCTACCCGCGTTAACGAt > 1:416136/1‑69 (MQ=255) tGCGATCTTGACGCGAGAGTATTCAACCTGTTCGCCTTCCAGGAAGTCGGAGCTACCCGCGTTAACGAt > 1:567980/1‑69 (MQ=255) tGCGATCTTGACGCGAGAGTATTCAACCTGTTCGCCTTCCAGGAAGTCGGAGCTACCCGCGTTAACGAt > 1:615595/1‑69 (MQ=255) tGCGATCTTGACGCGAGAGTATTCAACCTGTTCGCCTTCCAGGAAGTCGGAGCTACCCGCGTTAACGAt > 1:282217/1‑69 (MQ=255) tGCGATCTTGACGCGAGAGTATTCAACCTGTTCGCCTTCCAGGAAGTCGGAGCTACCCGCGTTAACGAt > 1:2647338/1‑69 (MQ=255) tGCGATCTTGACGCGAGAGTATTCAACCTGTTCGCCTTCCAGGAAGTCGGAGCTACCCGCGTTAACGAt > 1:2087608/1‑69 (MQ=255) tGCGATCTTGACGCGAGAGTATTCAACCTGTTCGCCTTCCAGGAAGTCGGAGCTACCCGCGTTAACGAt > 1:732212/1‑69 (MQ=255) tGCGATCTTGACGCGAGAGTATTCAACCTGTTCACCTTCCAGGAAGTCGGAGCTGCCCGCGTTAACGAt > 1:1650275/1‑69 (MQ=255) tGCGATCTTGACGCGAGAGTATTCAACCTGTTCACCTTCCAGGAAGTCGGAGCTGCCCGCGTTAACGAt > 1:505908/1‑69 (MQ=255) tGCGATCTTGACGCGAGAGTATTCAACCTGTTCACCTTCCAGGAAGTCGGAGCTGCCCGCGTTAACGAt > 1:3255269/1‑69 (MQ=255) tGCGATCTTGACGCGAGAGTATTCAACCTGTTCACCTTCCAGGAAGTCGGAGCTGCCCGCGTTAACGAt > 1:316636/1‑69 (MQ=255) tGCGATCTTGACGCGAGAGTATTCAACCTGTTCACCTTCCAGGAAGTCGGAGCTGCCCGCGTTAACGAt > 1:3054823/1‑69 (MQ=255) tGCGATCTTGACGCGAGAGTATTCAACCTGTTCACCTTCCAGGAAGTCGGAGCTGCCCGCGTTAACGAt > 1:2854605/1‑69 (MQ=255) tGCGATCTTGACGCGAGAGTATTCAACCTGTTCACCTTCCAGGAAGTCGGAGCTGCCCGCGTTAACGAt > 1:2243277/1‑69 (MQ=255) tGCGATCTTGACGCGAGAGTATTCAACCTGTTCACCTTCCAGGAAGTCGGAGCTGCCCGCGTTAACGAt > 1:2255898/1‑69 (MQ=255) gCGATCTTGACGCGAGAGTATTCAACCTGTTCGCCTTCCAGGAAGTCGGAGCTa > 1:2460774/1‑54 (MQ=255) gATCTTGACGCGAGAGTATTCAACCTGTTCGCCTTCCAGGAAGTCGGAGCTACCCGCGTTAACGATgg < 1:967596/68‑1 (MQ=255) cgcgAGAGTATTCAACCTGTTCGCCTTCCAGGAAGTCGGAGCTACCCGCGTTAACGATGGTAg < 1:1279703/63‑1 (MQ=255) cgcgAGAGTATTCAACCTGTTCGCCTTCCAGGAAGTCGGAGCTACCCGCGTTAACGATGGTAg < 1:975334/63‑1 (MQ=255) cgcgAGAGTATTCAACCTGTTCGCCTTCCAGGAAGTCGGAGCTACCCGCGTTAACGATGGTAg < 1:3379493/63‑1 (MQ=255) cgcgAGAGTATTCAACCTGTTCGCCTTCCAGGAAGTCGGAGCTACCCGCGTTAACGATGGTAg < 1:2134794/63‑1 (MQ=255) ttCAACCTGTTCGCCTTCCAGGAAGTCGGAGCTACCCGCGTTAACGATGGTAGCTTTACGCAGCATCTg < 1:2965633/69‑1 (MQ=255) tCAACCTGTTCGCCTTCCAGGAAGTCGGAGCTACCCGCGTTAACGATGGTAGATTTACGCAGCATCTg < 1:2067597/68‑1 (MQ=255) cAACCTGTTCGCCTTCCAGGAAGTCGGAGCTACCCGCGTTAACGATGGTAGCtt > 1:2951690/1‑54 (MQ=255) cAACCTGTTCGCCTTCCAGGAAGTCGGAGCTACCCGCGTTAACGATGGTAGCtt > 1:2939850/1‑54 (MQ=255) cAACCTGTTCGCCTTCCAGGAAGTCGGAGCTACCCGCGTTAACGATGGTAGCtt > 1:305125/1‑54 (MQ=255) cAACCTGTTCGCCTTCCAGGAAGTCGGAGCTACCCGCGTTAACGATGGTAGCtt > 1:935932/1‑54 (MQ=255) cAACCTGTTCGCCTTCCAGGAAGTCGGAGCTACCCGCGTTAACGATGGTAGCtt > 1:2850361/1‑54 (MQ=255) cAACCTGTTCGCCTTCCAGGAAGTCGGAGCTACCCGCGTTAACGATGGTAGCtt > 1:693155/1‑54 (MQ=255) cAACCTGTTCGCCTTCCAGGAAGTCGGAGCTACCCGCGTTAACGATGGTAGCtt > 1:2783725/1‑54 (MQ=255) cAACCTGTTCGCCTTCCAGGAAGTCGGAGCTACCCGCGTTAACGATGGTAGCTTTACGCAGCATCTGa > 1:1093756/1‑68 (MQ=255) cAACCTGTTCGCATTCCAGGAAGTCGGAGCTACCCGCGTTAACGATGGTAGCtt > 1:6863/1‑54 (MQ=255) cAACCTGTTCACCTTCCAGGAAGTCGGAGCTGCCCGCGTTAACGATGGTAGCtt < 1:1643327/54‑1 (MQ=39) cAACCTGTTCACCTTCCAGGAAGTCGGAGCTGCCCGCGTTAACGATGGTAGCtt < 1:1058222/54‑1 (MQ=39) cAACCTGTTCACCTTCCAGGAAGTCGGAGCTGCCCGCGTTAACGATGGTAGCtt < 1:1143887/54‑1 (MQ=39) cAACCTGTTCACCTTCCAGGAAGTCGGAGCTGCCCGCGTTAACGATGGTAGCtt < 1:97345/54‑1 (MQ=39) cAACCTGTTCACCTTCCAGGAAGTCGGAGCTGCCCGCGTTAACGATGGTAGCtt < 1:1112599/54‑1 (MQ=39) cAACCTGTTCACCTTCCAGGAAGTCGGAGCTGCCCGCGTTAACGATGGTAGCtt < 1:723022/54‑1 (MQ=39) cAACCTGTTCACCTTCCAGGAAGTCGGAGCTGCCCGCGTTAACGATGGTAGCtt < 1:2840394/54‑1 (MQ=39) cAACCTGTTCACCTTCCAGGAAGTCGGAGCTGCCCGCGTTAACGATGGTAGCtt < 1:2309609/54‑1 (MQ=39) cAACCTGTTCACCTTCCAGGAAGTCGGAGCTGCCCGCGTTAACGATGGTAGCtt < 1:2210829/54‑1 (MQ=39) cAACCTGTTCACCTTCCAGGAAGTCGGAGCTGCCCGCGTTAACGATGGTAGCtt < 1:2480463/54‑1 (MQ=39) cAACCTGTTCACCTTCCAGGAAGTCGGAGCTGCCCGCGTTAACGATGGTAGCtt < 1:2124489/54‑1 (MQ=39) cAACCTGTTCACCTTCCAGGAAGTCGGAGCTGCCCGCGTTAACGATGGTAGCtt < 1:2055187/54‑1 (MQ=39) cAACCTGTTCACCTTCCAGGAAGTCGGAGCTGCCCGCGTTAACGATGGTAGCtt < 1:296507/54‑1 (MQ=39) cAACCTGTTCACCTTCCAGGAAGTCGGAGCTGCCCGCGTTAACGATGGTAGCtt < 1:3017260/54‑1 (MQ=39) cAACCTGTTCACCTTCCAGGAAGTCGGAGCTGCCCGCGTTAACGATGGTAGCtt < 1:3262883/54‑1 (MQ=39) cAACCTGTTCACCTTCCAGGAAGTCGGAGCTGCCCGCGTTAACGATGGTAGCtt < 1:1864570/54‑1 (MQ=39) cAACCTGTTCACCTTCCAGGAAGTCGGAGCTGCCCGCGTTAACGATGGTAGCtt < 1:1873164/54‑1 (MQ=39) cAACCTGTTCACCTTCCAGGAAGTCGGAGCTGCCCGCGTTAACGATGGTAGCtt < 1:1929001/54‑1 (MQ=39) cAACCTGTTCACCTTCCAGGAAGTCGGAGCTGCCCGCGTTAACGATGGTAGCtt < 1:3059920/54‑1 (MQ=39) cTGTTCGCCTTCCAGGAAGTCGGAGCTACCCGCGtt > 1:2798553/1‑36 (MQ=255) cTGTTCGCCTTCCAGGAAGTCGGAGCTACCCGCGtt > 1:3239455/1‑36 (MQ=255) gTTCGCCTTCCAGGAAGTCGGAGCTACCCGCGTTAACGATGGTAGCTTTACGCAGCATCTGacgaacg > 1:2015141/1‑68 (MQ=255) cGCCTTCCAGGAAGTCGGAGCTACCCGCGTTAACGATGGTAGCTTTACGCAGCATCTGACGAACGAt < 1:730777/67‑1 (MQ=255) cGCCTTCCAGGAAGTCGGAGCTACCCGCGTTAACGATGGTAGCTTTACGCAGCATCTGACGAACGAt < 1:3396499/67‑1 (MQ=255) cGCCTTCCAGGAAGTCGGAGCTACCCGCGTTAACGATGGTAGCTTTACGCAGCATCTGACGAACGAt < 1:1097485/67‑1 (MQ=255) cGCCTTCCAGGAAGTCGGAGCTACCCGCGTTAACGATGGTAGCTTTACGCAGCATCTGACGAACGAt < 1:2110764/67‑1 (MQ=255) cGCCTTCCAGGAAGTCGGAGCTACCCGCGTTAACGATGGTAGCTTTACGCAGCATCTGACGAACGAt < 1:2546757/67‑1 (MQ=255) cGCCTTCCAGGAAGTCGGAGCTACCCGCGTTAACGATGGTAGCTTTACGCAGCATCTGACGAACGAt < 1:942664/67‑1 (MQ=255) cGCCTTCCAGGAAGTCGGAGCTACCCGCGTTAACGATGGTAGCTTTACGCAGCATCTGACGAACGAt < 1:1376385/67‑1 (MQ=255) cGCCTTCCAGGAAGTCGGAGCTACCCGCGTTAACGATGGTAGCTTTACGCAGCATCTGACGAACGAt < 1:654822/67‑1 (MQ=255) cGCCTTCCAGGAAGTCGGAGCTACCCGCGTTAACGATGGTAGCTTTACGCAGCATCTGACGAACGATaa > 1:636537/1‑69 (MQ=255) | CCCACTTTGCCGTTCGCTTCCAGTTCGCGGTTTGCGATCTTGACGCGAGAGTATTCAACCTGTTCGCCTTCCAGGAAGTCGGAGCTACCCGCGTTAACGATGGTAGCTTTACGCAGCATCTGACGAACGATAA > minE/2253142‑2253274 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |