| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,255,856 | A→G | 36.5% | I394I (ATT→ATC) | rpoC ← | RNA polymerase, beta prime subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,255,856 | 0 | A | G | 36.5% | 46.9 / 49.9 | 82 | I394I (ATT→ATC) | rpoC | RNA polymerase, beta prime subunit |
| Reads supporting (aligned to +/- strand): ref base A (26/26); new base G (23/7); total (49/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.08e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.30e-01 | |||||||||||
GATAACTTCGTCCAGGATATCCCAAACGACAGCTTCTTCGCGCTCAACCATTTTCTTCGCAGCTTTAATGGTGGTAGCAAGACCACGCAGTTCCAGCTTGCCGTAGATGAACGGTTTGAACAGC > minE/2255790‑2255913 | gATAACTTCGTCCAGGATATCCCAAACGACAGCTTCTTCGCGCTCAACCATTTTCTTCGCAGCTTTAAt > 1:1000078/1‑69 (MQ=255)gATAACTTCGTCCAGGATATCCCAAACGACAGCTTCTTCGCGCTCAACCATTTTCTTCGCAGCTTTAAt > 1:2250412/1‑69 (MQ=255) tCCAGGATATCCCAAACGACAGCTTCTTCGCGCTCAACCATTTTCTTCGCAGCTTTAATGGTGGTAGc < 1:199175/68‑1 (MQ=255) tCCAGGATATCCCAAACGACAGCTTCTTCGCGCTCAACCATTTTCTTCGCAGCTTTAATGGTGGTAGc < 1:1291080/68‑1 (MQ=255) ccAGGATATCCCAAACGACAGCTTCTTCGCGCTCAACCATTTTCTTCGCAGCTTTAATGGTGGTAGc < 1:1527188/67‑1 (MQ=255) ggATATCCCAAACGACAGCTTCTTCGCGCTCAACCATTTTCTTCGCAGCTTTAATGGTGGTAGCAAGAc < 1:2419753/69‑1 (MQ=255) gATATCCCAAACGACAGCTTCTTCGCGCTCAACCATTTTCTTCGCAGCTTTGATGGTGGTAGCaga > 1:168870/1‑64 (MQ=255) gATATCCCAAACGACAGCTTCTTCGCGCTCAACCATTTTCTTCGCAGCTTTGATGGTGGTAGCaa > 1:2788377/1‑65 (MQ=255) gATATCCCAAACGACAGCTTCTTCGCGCTCAACCATTTTCTTCGCAGCTTTGATGGTGGTAGCAAg > 1:1413260/1‑66 (MQ=255) gATATCCCAAACGACAGCTTCTTCGCGCTCAACCATTTTCTTCGCAGCTTTGATGGTGGTAGCAAGAcc > 1:2789753/1‑69 (MQ=255) gATATCCCAAACGACAGCTTCTTCGCGCTCAACCATTTTCTTCGCAGCTTTGATGGTGGTAGCAAGAcc > 1:3287340/1‑69 (MQ=255) gATATCCCAAACGACAGCTTCTTCGCGCTCAACCATTTTCTTCGCAGCTTTGATGGTGGTAGCAAGAcc > 1:1006534/1‑69 (MQ=255) gATATCCCAAACGACAGCTTCTTCGCGCTCAACCATTTTCTTCGCAGCTTTGATGGTGGTAGCAAGAcc > 1:2532488/1‑69 (MQ=255) gATATCCCAAACGACAGCTTCTTCGCGCTCAACCATTTTCTTCGCAGCTTTGATGGTGGTAGCAAGAcc > 1:2487846/1‑69 (MQ=255) gATATCCCAAACGACAGCTTCTTCGCGCTCAACCATTTTCTTCGCAGCTTTGATGGTGGTAGCAAGAcc > 1:380872/1‑69 (MQ=255) gATATCCCAAACGACAGCTTCTTCGCGCTCAACCATTTTCTTCGCAGCTTTGATGGTGGTAGCAAGAcc > 1:533891/1‑69 (MQ=255) gATATCCCAAACGACAGCTTCTTCGCGCTCAACCATTTTCTTCGCAGCTTTGATGGTGGTAGCAAGAcc > 1:606193/1‑69 (MQ=255) gATATCCCAAACGACAGCTTCTTCGCGCTCAACCATTTTCTTCGCAGCTTTGATGGTGGTAGCAAGAcc > 1:2146826/1‑69 (MQ=255) gATATCCCAAACGACAGCTTCTTCGCGCTCAACCATTTTCTTCGCAGCTTTGATGGTGGTAGCAAGAcc > 1:691362/1‑69 (MQ=255) gATATCCCAAACGACAGCTTCTTCGCGCTCAACCATTTTCTTCGCAGCTTTGATGGTGGTAGCAAGAcc > 1:985025/1‑69 (MQ=255) gATATCCCAAACGACAGCTTCTTCGCGCTCAACCATTTTCTTCGCAGCTTTGATGGTGGTAGCAAGAcc > 1:1973492/1‑69 (MQ=255) gATATCCCAAACGACAGCTTCTTCGCGCTCAACCATTTTCTTCGCAGCTTTGATGGTGGTAGCAAGAcc > 1:179047/1‑69 (MQ=255) gATATCCCAAACGACAGCTTCTTCGCGCTCAACCATTTTCTTCGCAGCTTTGATGGTGGTAGCAAGAcc > 1:1748191/1‑69 (MQ=255) gATATCCCAAACGACAGCTTCTTCGCGCTCAACCATTTTCTTCGCAGCTTTGATGGTGGTAGCAAGAcc > 1:1237671/1‑69 (MQ=255) gATATCCCAAACGACAGCTTCTTCGCGCTCAACCATTTTCTTCGCAGCTTTGATGGTGGTAGCAAGAc > 1:3108498/1‑68 (MQ=255) gATATCCCAAACGACAGCTTCTTCGCGCTCAACCATTTTCTTCGCAGCTTTGATGGTGGTAGCAAGAc > 1:3069852/1‑68 (MQ=255) gATATCCCAAACGACAGCTTCTTCGCGCTCAACCATTTTCTTCGCAGCTTTGATGGTGGTAGCAAGAc > 1:1394460/1‑68 (MQ=255) gATATCCCAAACGACAGCTTCTTCGCGCTCAACCATTTTCTTCGCAGCTTTGATGGTGGTAGCAAGAc > 1:667045/1‑68 (MQ=255) gATATCCCAAACGACAGCTTCTTCGCGCTCAACCATTTTCTTCGCAGCTTTGATGGTGGTAGCAAGAc > 1:939303/1‑68 (MQ=255) tatCCCAAACGACAGCTTCTTCGCGCTCAACCATTTTCTTCGCAGCTTTAATGGTGGTAGCAAGACCa > 1:3060649/1‑68 (MQ=255) tatCCCAAACGACAGCTTCTTCGCGCTCAACCATTTTCTTCGCAGCTTTAATGGTGGTAGCAAGACCa > 1:401663/1‑68 (MQ=255) tCCCAAACGACAGCTTCTTCGCGCTCAACCATTTTCTTCGCAGCTTTAATGGTGGTAGCAAGACCACGc > 1:831391/1‑69 (MQ=255) tCCCAAACGACAGCTTCTTCGCGCTCAACCATTTTCTTCGCAGCTTTAATGGTGGTAGCAAGACCACGc > 1:33781/1‑69 (MQ=255) tCCCAAACGACAGCTTCTTCGCGCTCAACCATTTTCTTCGCAGCTTTAATGGTGGTAGCAAGACCACGc > 1:3400056/1‑69 (MQ=255) tCCCAAACGACAGCTTCTTCGCGCTCAACCATTTTCTTCGCAGCTTTAATGGTGGTAGCAAGACCACGc > 1:1741751/1‑69 (MQ=255) tCCCAAACGACAGCTTCTTCGCGCTCAACCATTTTCTTCGCAGCTTTAATGGTGGTAGCAAGACCACGc > 1:2600326/1‑69 (MQ=255) tCCCAAACGACAGCTTCTTCGCGCTCAACCATTTTCTTCGCAGCTTTAATGGTGGTAGCAAGACCACGc > 1:2813935/1‑69 (MQ=255) tCCCAAACGACAGCTTCTTCGCGCTCAACCATTTTCTTCGCAGCTTTAATGGTGGTAGCAAGACCACGc > 1:2066640/1‑69 (MQ=255) tCCCAAACGACAGCTTCTTCGCGCTCAACCATTTTCTTCGCAGCTTTAATGGTGGTAGCAAGACCACGc > 1:2969517/1‑69 (MQ=255) tCCCAAACGACAGCTTCTTCGCGCTCAACCATTTTCTTCGCAGCTTTAATGGTGGTAGCAAGACCACGc > 1:1153051/1‑69 (MQ=255) tCCCAAACGACAGCTTCTTCGCGCTCAACCATTTTCTTCGCAGCTTTAATGGTGGTAGCAAGACCACGc > 1:573848/1‑69 (MQ=255) tCCCAAACGACAGCTTCTTCGCGCTCAACCATTTTCTTCGCAGCTTTAATGGTGGTAGCAAGACCACGc > 1:1079362/1‑69 (MQ=255) tCCCAAACGACAGCTTCTTCGCGCTCAACCATTTTCTTCGCAGCTTTAATGGTGGTAGCAAGACCACGc > 1:2094735/1‑69 (MQ=255) cccAAACGACAGCTTCTTCGCGCTCAACCATTTTCTTCGCAGCTTTAATGGTGGTAGc < 1:3335646/58‑1 (MQ=255) ccAAACGACAGCTTCTTCGCGCTCAACCATTTTCTTCGCAGCTTTAATGGTGGTAGCAAGACCACGCa > 1:2426932/1‑68 (MQ=255) ccAAACGACAGCTTCTTCGCGCTCAACCATTTTCTTCGCAGCTTTAATGGTGGTAGCAAGACCACGCa > 1:2442464/1‑68 (MQ=255) ccAAACGACAGCTTCTTCGCGCTCAACCATTTTCTTCGCAGCTTTAATGGTGGTAGCAAGACCACGCAg > 1:2403270/1‑69 (MQ=255) ccAAACGACAGCTTCTTCGCGCTCAACCATTTTCTTCGCAGCTTTAATGGTGGTAGCAAGACCACGCAg > 1:3240891/1‑69 (MQ=255) tcGCGCTCAACCATTTTCTTCGCAGCTTTAATGGTGGTAGCAAGACCACTCAGTTCCAGCTTGCCGTa < 1:2671392/68‑1 (MQ=255) tcGCGCTCAACCATTTTCTTCGCAGCTTTAATGGTGGTAGCAAGACCACGCAGTTCCAGCTTGCCGTa < 1:1519348/68‑1 (MQ=255) tcGCGCTCAACCATTTTCTTCGCAGCTTTAATGGTGGTAGCAAGACCACGCAGTTCCAGCTTGCCGTa < 1:2698822/68‑1 (MQ=255) tcGCGCTCAACCATTTTCTTCGCAGCTTTAATGGTGGTAGCAAGACCACGCAGTTCCAGCTTGCCGTa < 1:1986307/68‑1 (MQ=255) tcGCGCTCAACCATTTTCTTCGCAGCTTTAATGGTGGTAGCAAGACCACGCAGTTCCAGCTTGCCGTa < 1:397167/68‑1 (MQ=255) tcGCGCTCAACCATTTTCTTCGCAGCTTTAATGGTGGTAGCAAGACCACGCAGTTCCAGCTTGCCGTa < 1:1583934/68‑1 (MQ=255) tcGCGCTCAACCATTTTCTTCGCAGCTTTAATGGTGGTAGCAAGACCACGCAGTTCCAGCTTGCCGTa < 1:454481/68‑1 (MQ=255) tcGCGCTCAACCATTTTCTTCGCAGCTTTAATGGTGGTAGCAAGACCACGCAGTTCCAGCTTGCCGTa < 1:1673875/68‑1 (MQ=255) aCCATTTTCTTCGCAGCTTTAATGGTGGTAGCAAGACCACGCAGTTCCAGCTTGCCGTa < 1:872129/59‑1 (MQ=255) aTTTTCTTCGCAGCTTTAATGGTGGTAGCAAGACCACGCAGTTCCAGCTTGCCGTAGATGAACGGTTTg < 1:1048150/69‑1 (MQ=255) aTTTTCTTCGCAGCTTTAATGGTGGTAGCAAGACCACGCAGTTCCAGCTTGCCGTAGATGAACGGTTTg < 1:1673942/69‑1 (MQ=255) aTTTTCTTCGCAGCTTTAATGGTGGTAGCAAGACCACGCAGTTCCAGCTTGCCGTAGATGAACGGTTTg < 1:2385503/69‑1 (MQ=255) aTTTTCTTCGCAGCTTTAATGGTGGTAGCAAGACCACGCAGTTCCAGCTTGCCGTAGATGAACGGTTTg < 1:1405202/69‑1 (MQ=255) aTTTTCTTCGCAGCTTTAATGGGGGTAGCAAGACCACGCAGTTCCAGCTTGCCGTAGATGAACGGTTTg < 1:124873/69‑1 (MQ=255) ttcttcGCAGCTTTAATGGTGGTAGCAAGACCACGCAGTTCCAGCTTGCCGTAGATGAACGGTTTg > 1:2191682/1‑66 (MQ=255) ttcttcGCAGCTTTAATGGTGGTAGCAAGACCACGCAGTTCCAGCTTGCCGTAGATGAACGGTTTg > 1:2440243/1‑66 (MQ=255) ttcttcGCAGCTTTAATGGTGGTAGCAAGACCACGCAGTTCCAGCTTGCCGTAGATGAACGGTTTGAAc > 1:1454908/1‑69 (MQ=255) ttcttcGCAGCTTTAATGGTGGTAGCAAGACCACGCAGTTCCAGCTTGCCGTAGATGAACGGTTTGAAc > 1:3282640/1‑69 (MQ=255) ttcttcGCAGCTTTAATGGTGGTAGCAAGACCACGCAGTTCCAGCTTGCCGTAGATGAACGGTTTGAAc > 1:1665217/1‑69 (MQ=255) ttcttcGCAGCTTTAATGGTGGTAGCAAGACCACGCAGTTCCAGCTTGCCGTAGATGAACGGTTTGAAc > 1:752922/1‑69 (MQ=255) cttcGCAGCTTTAATGGTGGTAGCAAGACCACGCAGTTCCAGCTTGCCGTAGATGAACGGTTTGAACa < 1:18131/68‑1 (MQ=255) cttcGCAGCTTTAATGGTGGTAGCAAGACCACGCAGTTCCAGCTTGCCGTAGATGAACGGTTTGAACa < 1:2856760/68‑1 (MQ=255) cttcGCAGCTTTAATGGTGGTAGCAAGACCACGCAGTTCCAGCTTGCCGTAGATGAACGGTTTGAACa < 1:418857/68‑1 (MQ=255) cttcGCAGCTTTAATGGTGGTAGCAAGACCACGCAGTTCCAGCTTGCCGTAGATGAACGGTTTGAACa < 1:2360317/68‑1 (MQ=255) cttcGCAGCTTTAATGGTGGTAGCAAGACCACGCAGTTCCAGCTTGCCGTAGATGAACGGTTTGAACa < 1:2202061/68‑1 (MQ=255) tcGCAGCTTTGATGGTGGTAGCAAGACCACGCAGTTCCAGCTTGCCGTAGATGAACGGTTTGAACAGc < 1:888719/68‑1 (MQ=255) tcGCAGCTTTGATGGTGGTAGCAAGACCACGCAGTTCCAGCTTGCCGTAGATGAACGGTTTGAACAGc < 1:1772533/68‑1 (MQ=255) tcGCAGCTTTGATGGTGGTAGCAAGACCACGCAGTTCCAGCTTGCCGTAGATGAACGGTTTGAACAGc < 1:2299378/68‑1 (MQ=255) tcGCAGCTTTGATGGTGGTAGCAAGACCACGCAGTTCCAGCTTGCCGTAGATGAACGGTTTGAACAGc < 1:1098889/68‑1 (MQ=255) tcGCAGCTTTGATGGTGGTAGCAAGACCACGCAGTTCCAGCTTGCCGTAGATGAACGGTTTGAACAGc < 1:3157570/68‑1 (MQ=255) tcGCAGCTTTGATGGTGGTAGCAAGACCACGCAGTTCCAGCTTGCCGTAGATGAACGGTTTGAACAGc < 1:2758899/68‑1 (MQ=255) tcGCAGCTTTGATGGTGGTAGCAAGACCACGCAGTTCCAGCTTGCCGTAGATGAACGGTTTGAACAGc < 1:294623/68‑1 (MQ=255) tcGCAGCTTTAATGGTGGTAGCAAGACCACGCAGTTCCAGCTTGCCGTAGATGAACGGTTTGAACAGc < 1:659113/68‑1 (MQ=255) cGCAGCTTTAATGGTGGTAGCAAGACCACGCAGTTCCAGCTTGCCGTAGATGAACGGTTTGAACAGc < 1:2084753/67‑1 (MQ=255) | GATAACTTCGTCCAGGATATCCCAAACGACAGCTTCTTCGCGCTCAACCATTTTCTTCGCAGCTTTAATGGTGGTAGCAAGACCACGCAGTTCCAGCTTGCCGTAGATGAACGGTTTGAACAGC > minE/2255790‑2255913 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |