| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 190,114 | T→C | 62.6% | F537F (TTT→TTC) | ldcC → | lysine decarboxylase 2, constitutive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 190,114 | 0 | T | C | 62.6% | 48.7 / 70.0 | 112 | F537F (TTT→TTC) | ldcC | lysine decarboxylase 2, constitutive |
| Reads supporting (aligned to +/- strand): ref base T (17/25); new base C (45/25); total (62/50) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.86e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01 | |||||||||||
Only 100 of 113 total aligned reads displayed.
GCAAAATTCCTCGACGAACGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTGGGTTGACGGAATTCAAACGC > minE/190046‑190177 | gCAAAATTCCTCGACGAACGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCttt < 1:2359602/69‑1 (MQ=255) aaTACCTCGACGCACATGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAg > 1:3303229/1‑67 (MQ=255) aaTACCTCGACGAACATTGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAgca < 1:206573/69‑3 (MQ=255) aaTACCTCGACGAACATGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAgca < 1:1468180/69‑3 (MQ=255) aaTACCTCGACGAACATGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAgca < 1:1904420/69‑3 (MQ=255) aaTACCTCGACGAACATGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAgca < 1:1888849/69‑3 (MQ=255) aaTACCTCGACGAACATGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAgca < 1:1846561/69‑3 (MQ=255) aaTACCTCGACGAACATGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAgca < 1:2072512/69‑3 (MQ=255) aaTACCTCGACGAACATGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAgca < 1:3077589/69‑3 (MQ=255) aaTACCTCGACGAACATGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAgca < 1:2926966/69‑3 (MQ=255) aaTACCTCGACGAACATGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAgca < 1:1607158/69‑3 (MQ=255) aaTACCTCGACGAACATGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAgca < 1:3224580/69‑3 (MQ=255) aaTACCTCGACGAACATGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAgca < 1:2151660/69‑3 (MQ=255) aaTACCTCGACGAACATGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAgca < 1:3314474/69‑3 (MQ=255) aaTACCTCGACGAACATGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAgca < 1:953241/69‑3 (MQ=255) aaTACCTCGACGAACATGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAgca < 1:3352934/69‑3 (MQ=255) aaTACCTCGACGAACATGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAgca < 1:1419785/69‑3 (MQ=255) aaTACCTCGACGAACATGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAgca < 1:2854087/69‑3 (MQ=255) aaTACCTCGACGAACATGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAgca < 1:2842915/69‑3 (MQ=255) aaTACCTCGACGAACATGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAgca < 1:2403666/69‑3 (MQ=255) aaTACCTCGACGAACATGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAgca < 1:870975/69‑3 (MQ=255) aaTACCTCGACGAACATGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAgca < 1:1233416/69‑3 (MQ=255) aaTACCTCGACGAACATGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAgca < 1:592151/69‑3 (MQ=255) aaTACCTCGACGAACATGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAgca < 1:616781/69‑3 (MQ=255) aaTACCTCGACGAACATGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAgca < 1:2668939/69‑3 (MQ=255) aaTACCTCGACGAACATGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAgca < 1:2663649/69‑3 (MQ=255) aaTACCTCGACGAACATGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAg > 1:209702/1‑67 (MQ=255) aaTACCTCGACGAACATGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAg > 1:2017844/1‑67 (MQ=255) aaTACCTCGACGAACATGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAg > 1:2225287/1‑67 (MQ=255) aaTACCTCGACGAACATGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAg > 1:211024/1‑67 (MQ=255) aaTACCTCGACGAACATGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAg > 1:2485818/1‑67 (MQ=255) aaTACCTCGACGAACATGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAg > 1:3028215/1‑67 (MQ=255) aaTACCTCGACGAACATGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAg > 1:1791176/1‑67 (MQ=255) aaTACCTCGACGAACATGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAg > 1:3183370/1‑67 (MQ=255) aaTACCTCGACGAACATGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAg > 1:3243803/1‑67 (MQ=255) aaTACCTCGACGAACATGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAg > 1:1528336/1‑67 (MQ=255) aaTACCTCGACGAACATGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAg > 1:3317817/1‑67 (MQ=255) aaTACCTCGACGAACATGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAg > 1:397673/1‑67 (MQ=255) aaTACCTCGACGAACATGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAg > 1:1307497/1‑67 (MQ=255) aaTACCTCGACGAACATGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAg > 1:1278889/1‑67 (MQ=255) aaTACCTCGACGAACATGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAg > 1:838202/1‑67 (MQ=255) aaTACCTCGACGAACATGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAg > 1:114925/1‑67 (MQ=255) agagAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGc < 1:3073395/63‑1 (MQ=255) agagAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGc < 1:2927428/63‑1 (MQ=255) agagAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGc < 1:3201208/63‑1 (MQ=255) agagAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGc < 1:3422104/63‑1 (MQ=255) agagAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGc < 1:1040917/63‑1 (MQ=255) agagAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGc < 1:1097696/63‑1 (MQ=255) agagAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGc < 1:152510/63‑1 (MQ=255) gagaAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATggg > 1:849883/1‑68 (MQ=255) gagaAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATggg > 1:1655277/1‑68 (MQ=255) gagaAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATggg > 1:65620/1‑68 (MQ=255) gagaAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATggg > 1:1583036/1‑68 (MQ=255) gagaAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATggg > 1:580706/1‑68 (MQ=255) gaAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGat < 1:3028590/68‑1 (MQ=255) taACCTGCTGTTCCTGTTCAGCATCGGTATCGATAAGACCaa > 1:52375/1‑42 (MQ=12) taACCTGCTGTTCCTGTTCAGCATCGGTATCGATAAGACCaa > 1:3093203/1‑42 (MQ=12) taACCTGCTGTTCCTGTTCAGCATCGGTATCGATAAGACCaa > 1:1729141/1‑42 (MQ=12) taACCTGCTGTTCCTGTTCAGCATCGGTATCGATAAGACCaa > 1:1571990/1‑42 (MQ=12) taACCTGCTGTTCCTGTTCAGCATCGGTATCGATAAGACCaa > 1:1459043/1‑42 (MQ=12) taACCTGCTGTTCCTGTTCAGCATCGGTATCGATAAGACCaa > 1:1443292/1‑42 (MQ=12) taACCTGCTGTTCCTGTTCAGCATCGGTATCGATAAGACCaa > 1:1024320/1‑42 (MQ=12) taACCTGCTGTTCCTGTTCAGCATCGGTATCGATAAGACCaa > 1:119022/1‑42 (MQ=12) taACCTGCTGTTCCTGTTCAGCATCGGTATCGATAAGACCaa > 1:1169000/1‑42 (MQ=12) taACCTGCTGTTCCTGTTCAGCATCGGTATCGATAAGACCaa > 1:726003/1‑42 (MQ=12) taACCTGCTGTTCCTGTTCAGCATCGGTATCGATAAGACCaa > 1:1152916/1‑42 (MQ=12) taACCTGCTGTTCCTGTTCAGCATCGGTATCGATAAGACCaa > 1:2508104/1‑42 (MQ=12) taACCTGCTGTTCCTGTTCAGCATCGGTATCGATAAGACCaa > 1:1851622/1‑42 (MQ=12) taACCTGCTGTTCCTGTTCAGCATCGGTATCGATAAGACCaa > 1:2631109/1‑42 (MQ=12) taACCTGCTGTTCCTGTTCAGCATCGGTATCGATAAGACCaa > 1:2724801/1‑42 (MQ=12) taACCTGCTGTTCCTGTTCAGCATCGGTATCGATAAGACCaa > 1:2336588/1‑42 (MQ=12) taACCTGCTGTTCCTGTTCAGCATCGGTATCGATAAGACCaa > 1:2889633/1‑42 (MQ=12) taACCTGCTGTTCCTGTTCAGCATCGGTATCGATAAGACCaa > 1:1898445/1‑42 (MQ=12) taACCTGCTGTTCCTGTTCAGCATCGGTATCGATAAGACCaa > 1:2076834/1‑42 (MQ=12) gTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTGGGTTGACGGAATTCaa > 1:457121/1‑69 (MQ=255) tctcTTTAGTATTGGCATCGATAAAACCAAAGCAATggg < 1:2389604/39‑1 (MQ=255) tctcTTTAGTATTGGCATCGATAAAACCAAAGCAATggg < 1:2519936/39‑1 (MQ=255) tctcTTTAGTATTGGCATCGATAAAACCAAAGCAATggg < 1:880346/39‑1 (MQ=255) tctcTTTAGTATTGGCATCGATAAAACCAAAGCAATggg < 1:3061172/39‑1 (MQ=255) tctcTTTAGTATTGGCATCGATAAAACCAAAGCAATggg < 1:844963/39‑1 (MQ=255) tctcTTTAGTATTGGCATCGATAAAACCAAAGCAATggg < 1:720310/39‑1 (MQ=255) tctcTTTAGTATTGGCATCGATAAAACCAAAGCAATggg < 1:208881/39‑1 (MQ=255) tctcTTTAGTATTGGCATCGATAAAACCAAAGCAATggg < 1:1606807/39‑1 (MQ=255) tctcTTTAGTATTGGCATCGATAAAACCAAAGCAATggg < 1:1350954/39‑1 (MQ=255) tctcTTTAGTATTGGCATCGATAAAACCAAAGCAATggg < 1:2911369/39‑1 (MQ=255) tctcTTTAGTATTGGCATCGATAAAACCAAAGCAATggg < 1:3227026/39‑1 (MQ=255) tctcTTTAGTATTGGCATCGATAAAACCAAAGCAATggg < 1:3401470/39‑1 (MQ=255) tctcTTTAGTATTGGCATCGATAAAACCAAAGCAATggg < 1:1767828/39‑1 (MQ=255) tctcTTTAGTATTGGCATCGATAAAACCAAAGCAATggg < 1:375301/39‑1 (MQ=255) tctcTTTAGTATTGGCATCGATAAAACCAAAGCAATggg < 1:1321500/39‑1 (MQ=255) tctcTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTg > 1:2413438/1‑50 (MQ=255) tctcTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTg > 1:680247/1‑50 (MQ=255) tctcTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTg > 1:825791/1‑50 (MQ=255) tctcTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTg > 1:3386938/1‑50 (MQ=255) ctctTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTGGGTTGACGGAATTCAAACGc > 1:3055574/1‑69 (MQ=255) ctctTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTGGGTTGACGGAATTCAAACGc > 1:2423757/1‑69 (MQ=255) ctctTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTGGGTTGACGGAATTCAAACGc > 1:59188/1‑69 (MQ=255) ctctTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTGGGTTGACGGAATTCAAACGc > 1:2613932/1‑69 (MQ=255) ctctTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTGGGTTGACGGAATTCAAACGc > 1:2884850/1‑69 (MQ=255) ctctTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTCGGTTGACGGAATTCAAACGc > 1:2652992/1‑69 (MQ=255) | GCAAAATTCCTCGACGAACGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTGGGTTGACGGAATTCAAACGC > minE/190046‑190177 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |