| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,258,470 | A→G | 50.9% | G891G (GGT→GGC) | rpoB ← | RNA polymerase, beta subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,258,470 | 0 | A | G | 50.9% | ‑1.8 / 47.2 | 55 | G891G (GGT→GGC) | rpoB | RNA polymerase, beta subunit |
| Reads supporting (aligned to +/- strand): ref base A (10/17); new base G (12/16); total (22/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.85e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01 | |||||||||||
GGCTTTCTCACCGAAGATCGCACGCAGCAGTTTTTCTTCTGGGGTCAGCTGAGTTTCACCTTTCGGCGTTACCTTACCAACCAGAATGTCGCCACCGGTCACTTCCGCACCAATGTAAAC > minE/2258413‑2258532 | ggCTTTCTCACCGAAGATCGCACGCAGCAGTTTTTCTTCTGGGGTCAGCTGAGTTTCACCTTTCGGCGt < 1:2030158/69‑1 (MQ=255) ttttCTTCTGGGGTCAGCTGAGTTTCGCCTTTCGGCGTTACCTTACCAACCAGAATGTCGCCACCGGTc > 1:791475/1‑69 (MQ=255) ttttCTTCTGGGGTCAGCTGAGTTTCGCCTTTCGGCGTTACCTTACCAACCAGAATGTCGCCACCGGTc > 1:730928/1‑69 (MQ=255) ttttCTTCTGGGGTCAGCTGAGTTTCGCCTTTCGGCGTTACCTTACCAACCAGAATGTCGCCACCGGTc > 1:1864962/1‑69 (MQ=255) ttttCTTCTGGGGTCAGCTGAGTTTCGCCTTTCGGCGTTACCTTACCAACCAGAATGTCGCCACCGGTc > 1:1546987/1‑69 (MQ=255) ttttCTTCTGGGGTCAGCTGAGTTTCGCCTTTCGGCGTTACCTTACCAACCAGAATGTCGCCACCGGTc > 1:1394935/1‑69 (MQ=255) ttttCTTCTGGGGTCAGCTGAGTTTCGCCTTTCGGCGTTACCTTACCAACCAGAATGTCGCCACCGGTc > 1:2972147/1‑69 (MQ=255) ttttCTTCTGGGGTCAGCTGAGTTTCGCCTTTCGGCGTTACCTTACCAACCAGAATGTCGCCACCGGTc > 1:636142/1‑69 (MQ=255) ttttCTTCTGGGGTCAGCTGAGTTTCGCCTTTCGGCGTTACCTTACCAACCAGAATGTCGCCACCGGTc > 1:1189553/1‑69 (MQ=255) ttttCTTCTGGGGTCAGCTGAGTTTCGCCTTTCGGCGTTACCTTACCAACCAGAATGTCGCCACCGGTc > 1:1169690/1‑69 (MQ=255) ttttCTTCTGGGGTCAGCTGAGTTTCGCCTTTCGGCGTTACCTTACCAACCAGAATGTCGCCACCGGTc > 1:3400202/1‑69 (MQ=255) ttttCTTCTGGGGTCAGCTGAGTTTCGCCTTTCGGCGTTACCTTACCAACCAGAATGTCGCCACCGGTc > 1:952607/1‑69 (MQ=255) ttttCTTCTGGGGTCAGCTGAGTTTCGCCTTTCGGCGTTACCTTACCAACCAGAATGTCGCCACCGGTc > 1:367644/1‑69 (MQ=255) tttCTTCTGGGGTCAGCTGAGTTTCACCTTTCGGCGTTACCTTACCAACCAGAATGTCGCCACCGGTCa > 1:946257/1‑69 (MQ=255) tttCTTCTGGGGTCAGCTGAGTTTCACCTTTCGGCGTTACCTTACCAACCAGAATGTCGCCACCGGTCa > 1:2944380/1‑69 (MQ=255) tttCTTCTGGGGTCAGCTGAGTTTCACCTTTCGGCGTTACCTTACCAACCAGAATGTCGCCACCGGTCa > 1:2765451/1‑69 (MQ=255) tttCTTCTGGGGTCAGCTGAGTTTCACCTTTCGGCGTTACCTTACCAACCAGAATGTCGCCACCGGTCa > 1:589535/1‑69 (MQ=255) ctGGGGTCAGCTGAGTTTCACCTTTCGGCGTTACCTTACCAACCAGAATGTCGCCACCGGt < 1:250389/61‑1 (MQ=255) ctGGGGTCAGCTGAGTTTCACCTTTCGGCGTTACCTTACCAACCAGAATGTCGCCACCGGt < 1:2456291/61‑1 (MQ=255) ctGGGGTCAGCTGAGTTTCACCTTTCGGCGTTACCTTACCAACCAGAATGTCGCCACCGGt < 1:2406835/61‑1 (MQ=255) gTCAGCTGAGTTTCACCTTTCGGCGTTACCTTACCAACCAGAATGTCGCCACCGGt < 1:1008825/56‑1 (MQ=255) gTCAGCTGAGTTTCACCTTTCGGCGTTACCTTACCAACCAGAATGTCGCCACCGGTCACTTCCGCAcc > 1:3114299/1‑68 (MQ=255) gTCAGCTGAGTTTCACCTTTCGGCGTTACCTTACCAACCAGAATGTCGCCACCGGTCACTTCCGCAcc > 1:1554200/1‑68 (MQ=255) gTCAGCTGAGTTTCACCTTTCGGCGTTACCTTACCAACCAGAATGTCGCCACCGGTCACTTCCGCACCa > 1:1924240/1‑69 (MQ=255) gTCAGCTGAGTTTCACCTTTCGGCGTTACCTTACCAACCAGAATGTCGCCACCGGTCACTTCCGCACCa < 1:1056600/69‑1 (MQ=255) gTCAGCTGAGTTTCACCTTTCGGCGTTACCTTACCAACCAGAATGTCGCCACCGGTCACTTCCGCACCa < 1:1226641/69‑1 (MQ=255) gTCAGCTGAGTTTCACCTTTCGGCGTTACCTTACCAACCAGAATGTCGCCACCGGTCACTTCCGCACCa < 1:3206734/69‑1 (MQ=255) gTCAGCTGAGTTTCACCTTTCGGCGTTACCTTACCAACCAGAATGTCGCCACCGGTCACTTCCGCACCa > 1:1723185/1‑69 (MQ=255) gTCAGCTGAGTTTCACCTTTCGGCGTTACCTTACCAACCAGAATGTCGCCACCGGTCACTTCCGCACCa < 1:1861327/69‑1 (MQ=255) tCAGCTGAGTTTCACCTTTCGGCGTTACCTTACCAACCAGAATGTCGCCACCGGTCACTTCCGCACCaa > 1:358460/1‑69 (MQ=255) tCAGCTGAGTTTCACCTTTCGGCGTTACCTTACCAACCAGAATGTCGCCACCGGTCACTTCCGCACCaa > 1:2804611/1‑69 (MQ=255) gAGTTTCGCCTTTCGGCGTTACCTTACCAACCAGAATGTCGCCACCGGTCACTTcc < 1:2682396/56‑1 (MQ=255) gAGTTTCGCCTTTCGGCGTTACCTTACCAACCAGAATGTCGCCACCGGTCACTTcc < 1:1040196/56‑1 (MQ=255) gAGTTTCGCCTTTCGGCGTTACCTTACCAACCAGAATGTCGCCACCGGTCACTTcc < 1:1974317/56‑1 (MQ=255) gAGTTTCGCCTTTCGGCGTTACCTTACCAACCAGAATGTCGCCACCGGTCACTTcc < 1:3386714/56‑1 (MQ=255) gAGTTTCGCCTTTCGGCGTTACCTTACCAACCAGAATGTCGCCACCGGTCACTTcc < 1:2098898/56‑1 (MQ=255) gAGTTTCGCCTTTCGGCGTTACCTTACCAACCAGAATGTCGCCACCGGTCACTTcc < 1:3239640/56‑1 (MQ=255) gAGTTTCGCCTTTCGGCGTTACCTTACCAACCAGAATGTCGCCACCGGTCACTTcc < 1:1843980/56‑1 (MQ=255) gAGTTTCGCCTTTCGGCGTTACCTTACCAACCAGAATGTCGCCACCGGTCACTTcc < 1:3056986/56‑1 (MQ=255) gAGTTTCGCCTTTCGGCGTTACCTTACCAACCAGAATGTCGCCACCGGTCACTTcc < 1:3038398/56‑1 (MQ=255) gAGTTTCGCCTTTCGGCGTTACCTTACCAACCAGAATGTCGCCACCGGTCACTTcc < 1:1233057/56‑1 (MQ=255) gAGTTTCGCCTTTCGGCGTTACCTTACCAACCAGAATGTCGCCACCGGTCACTTcc < 1:69106/56‑1 (MQ=255) gAGTTTCGCCTTTCGGCGTTACCTTACCAACCAGAATGTCGCCACCGGTCACTTcc < 1:730169/56‑1 (MQ=255) gAGTTTCGCCTTTCGGCGTTACCTTACCAACCAGAATGTCGCCACCGGTCACTTcc < 1:1573066/56‑1 (MQ=255) gAGTTTCGCCTTTCGGCGTTACCTTACCAACCAGAATGTCGCCACCGGTCACTTcc < 1:2600320/56‑1 (MQ=255) gAGTTTCGCCTTTCGGCGTTACCTTACCAACCAGAATGTCGCCACCGGTAACTTcc < 1:1026688/56‑1 (MQ=39) gAGTATCGCCATTCGGCGTTACCTTACCAACCAGAATGTCGCCACCGGTCACTTcc < 1:998646/56‑1 (MQ=255) gTTTCACCTTTCGGCGTTACCTTACCAACCAGAATGTCGCCACCGGTCACTTCCGCACCa < 1:2831531/60‑1 (MQ=255) tttCACCTTTCGGCGTTACCTTACCAACCAGAATGTCGCCACCGGTCACTTCCGCACCAATGTAAAc < 1:331264/67‑1 (MQ=255) tttCACCTTTCGGCGTTACCTTACCAACCAGAATGTCGCCACCGGTCACTTCCGCACCAATGTAAAc < 1:2887901/67‑1 (MQ=255) tttCACCTTTCGGCGTTACCTTACCAACCAGAATGTCGCCACCGGTCACTTCCGCACCAATGTAAAc < 1:2045209/67‑1 (MQ=255) tttCACCTTTCGGCGTTACCTTACCAACCAGAATGTCGCCACCGGTCACTTCCGCACCAATGTAAAc < 1:754996/67‑1 (MQ=255) tttCACCTTTCGGCGTTACCTTACCAACCAGAATGTCGCCACCGGTCACTTCCGCACCAATGTAAAc < 1:1394130/67‑1 (MQ=255) tttCACCTTTCGGCGTTACCTTACCAACCAGAATGTCGCCACCGGTCACTTCCGCACCAATGTAAAc < 1:118263/67‑1 (MQ=255) tttCACCTTTCGGCGTTACCTTACCAACCAGAATGTCGCCACCGGTCACTTCCGCACCAATGTAAAc < 1:1045080/67‑1 (MQ=255) | GGCTTTCTCACCGAAGATCGCACGCAGCAGTTTTTCTTCTGGGGTCAGCTGAGTTTCACCTTTCGGCGTTACCTTACCAACCAGAATGTCGCCACCGGTCACTTCCGCACCAATGTAAAC > minE/2258413‑2258532 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |