| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,259,276 | A→G | 27.7% | L623L (TTG→CTG) | rpoB ← | RNA polymerase, beta subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,259,276 | 0 | A | G | 27.7% | 186.3 / 77.3 | 173 | L623L (TTG→CTG) | rpoB | RNA polymerase, beta subunit |
| Reads supporting (aligned to +/- strand): ref base A (73/52); new base G (21/27); total (94/79) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.10e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01 | |||||||||||
Only 100 of 173 total aligned reads displayed.
ACAAGCTGGATTCGCCTTTGCTACGGCAAGTTACCAGGTCTTCTACGAAGTGGCCTTCTTCATCCAAGTTGGAGTTCGCCTGGGCGATAACGTAGTTGCCTTCTTCGATAGCAGACAGGTAGTGAATTTCGTC > minE/2259210‑2259342 | aCAAGCTGGATTCGCCTTTGCTACGGCAAGTTACCAGGTCTTCTACGAAGTGGCCTTCTTCATCCAAGt > 1:164628/1‑69 (MQ=255)aCAAGCTGGATTCGCCTTTGCTACGGCAAGTTACCAGGTCTTCTACGAAGTGGCCTTCTTCATCCAAGt > 1:1835174/1‑69 (MQ=255)aCAAGCTGGATTCGCCTTTGCTACGGCAAGTTAACAGGTCTTCTACGAAGTGGCCTTCTTCATCCAAGt > 1:821919/1‑69 (MQ=255)aCAAGCTGGATTCGCCTTTGCTACGGCAAGTTAACAGGTCTTCTACGAAGTGGCCTTCTTCATCCAAGt > 1:1262736/1‑69 (MQ=255) ccTTTGCTACGGCAAGTTACCAGGTCTTCTACGAAGTGGCCTTCTTCATCCAAGTTGGAGTTCGCCTg < 1:1229284/68‑1 (MQ=255) aCGGCAAGTTACCAGGTCTTCTACGAAGTGGCCTTCTTCATCCAAGTTGGAGTTCGCCTGGGCGATaa < 1:3040028/68‑1 (MQ=255) aCGGCAAGTTACCAGGTCTTCTACGAAGTGGCCTTCTTCATCCAAGTTGGAGTTCGCCTGGGCGATaa < 1:113056/68‑1 (MQ=255) aCGGCAAGTTACCAGGTCTTCTACGAAGTGGCCTTCTTCATCCAAGTTGGAGTTCGCCTGGGCGATaa < 1:1156132/68‑1 (MQ=255) aCGGCAAGTTACCAGGTCTTCTACGAAGTGGCCTTCTTCATCCAAGTTGGAGTTCGCCTGGGCGATaa < 1:1762585/68‑1 (MQ=255) gCAAGTTACCAGGTCTTCTACGAAGTGGCCTTCTTCATCCAAGTTGGAGTTCGCCTGGGCGATAACg < 1:119570/67‑1 (MQ=255) gCAAGTTACCAGGTCTTCTACGAAGTGGCCTTCTTCATCCAAGTTGGAGTTCGCCTGGGCGATAACg < 1:2785594/67‑1 (MQ=255) gCAAGTTACCAGGTCTTCTACGAAGTGGCCTTCTTCATCCAAGTTGGAGTTCGCCTGGGCGATAACg < 1:1805867/67‑1 (MQ=255) gCAAGTTACCAGGTCTTCTACGAAGTGGCCTTCTTCATCCAAGTTGGAGTTCGCCTGGGCGATAACg < 1:2150275/67‑1 (MQ=255) gCAAGTTACCAGGTCTTCTACGAAGTGGCCTTCTTCATCCAAGTTGGAGTTCGCCTGGGCGATAACGTa < 1:1550073/69‑1 (MQ=255) gCAAGTTACCAGGTCTTCTACGAAGTGGCCTTCTTCATCCAAGTTGGAGTTCGCCTGGGCGATAACGTa < 1:1057932/69‑1 (MQ=255) gCAAGTTACCAGGTCTTCTACGAAGTGGCCTTCTTCATCCAAGTTGGAGTTCGCCTGGGCGATAACGTa < 1:2276581/69‑1 (MQ=255) gCAAGTTACCAGGTCTTCTACGAAGTGGCCTTCTTCATCCAAGTTGGAGTTCGCCTGGGCGATAACGTa < 1:2663219/69‑1 (MQ=255) gCAAGTTACCAGGTCTTCTACGAAGTGGCCTTCTTCATCCAAGTTGGAGTTCGCCTGGGCGATAACGTa < 1:2952600/69‑1 (MQ=255) gCAAGTTACCAGGTCTTCTACGAAGTGGCCTTCTTCATCCAAGTTGGAGTTCGCCTGGGCGATAACGTa < 1:989654/69‑1 (MQ=255) gCAAGTTACCAGGTCTTCTACGAAGTGGCCTTCTTCATCCAAGTTGGAGTTCGCCTGGGCGATAACGTa < 1:1474248/69‑1 (MQ=255) gCAAGTTACCAGGTCTTCTACGAAGTGGCCTTCTTCATCCAAGTTGGAGTTCGCCTGGGCGATAACGTa < 1:1534394/69‑1 (MQ=255) gCAAGTTACCAGGTCTTCTACGAAGTGGCCTTCTTCATCCAAGTTGGAGTTCGCCTGGGCGATAACGTa < 1:1117156/69‑1 (MQ=255) gCAAGTTACCAGGTCTTCTACGAAGTGGCCTTCTTCATCCAAGTT‑GAGTTCGCCTGGGCGATAACGTa < 1:601598/68‑1 (MQ=255) aaGTTACCAGGTCTTCTACGAAGTGGCCTTCTTCATCCAAGTTGGAGTTCGccgg < 1:2595406/55‑3 (MQ=255) tACCAGGTCTTCTACGAAGTGGCCTTCTTCATCCAGGTTGGAGtt > 1:201833/1‑45 (MQ=255) tACCAGGTCTTCTACGAAGTGGCCTTCTTCATCCAGGTTGGAGTTCGCCTGGGCGATAACGt > 1:2548647/1‑62 (MQ=255) tACCAGGTCTTCTACGAAGTGGCCTTCTTCATCCAGGTTGGAGTTCGCCTGGGCGATAACGTa > 1:2482373/1‑63 (MQ=255) tACCAGGTCTTCTACGAAGTGGCCTTCTTCATCCAGGTTGGAGTTCGCCTGGGCGATAACGTa > 1:2493574/1‑63 (MQ=255) tACCAGGTCTTCTACGAAGTGGCCTTCTTCATCCAGGTTGGAGTTCGCCTGGGCGATAACGTa > 1:2557462/1‑63 (MQ=255) tACCAGGTCTTCTACGAAGTGGCCTTCTTCATCCAGGTTGGAGTTCGCCTGGGCGATAACGTa > 1:1373826/1‑63 (MQ=255) tACCAGGTCTTCTACGAAGTGGCCTTCTTCATCCAGGTTGGAGTTCGCCTGGGCGATAACGTa > 1:2691177/1‑63 (MQ=255) tACCAGGTCTTCTACGAAGTGGCCTTCTTCATCCAGGTTGGAGTTCGCCTGGGCGATAACGTa > 1:2884511/1‑63 (MQ=255) tACCAGGTCTTCTACGAAGTGGCCTTCTTCATCCAGGTTGGAGTTCGCCTGGGCGATAACGTa > 1:3192783/1‑63 (MQ=255) tACCAGGTCTTCTACGAAGTGGCCTTCTTCATCCAGGTTGGAGTTCGCCTGGGCGATAACGTa > 1:3370389/1‑63 (MQ=255) tACCAGGTCTTCTACGAAGTGGCCTTCTTCATCCAGGTTGGAGTTCGCCTGGGCGATAACGTa > 1:791395/1‑63 (MQ=255) tACCAGGTCTTCTACGAAGTGGCCTTCTTCATCCAGGTTGGAGTTCGCCTGGGCGATAACGTa > 1:915931/1‑63 (MQ=255) tACCAGGTCTTCTACGAAGTGGCCTTCTTCATCCAGGTTGGAGTTCGCCTGGGCGATAACGTa > 1:946590/1‑63 (MQ=255) tACCAGGTCTTCTACGAAGTGGCCTTCTTCATCCAGGTTGGAGTTCGCCTGGGCGATAAAGTa > 1:2717345/1‑63 (MQ=255) tACCAGGTCTTCTACGAAGTGGCCTTCTTCATCCAGGTTGGAGTTCGCCTGGGAGATAACGTa > 1:1491110/1‑63 (MQ=255) tACCAGGTCTTCTACGAAGTGGCCTTCTTCATCCAGGTTGGAGTTCGACTGGGCGATAACGTa > 1:2453049/1‑63 (MQ=255) tACCAGGTCTTCTACGAAGTGGCCTTCTTCATCCAAGTTGGAGTTCGCCTGGGCGATAACGTAGTTGcc > 1:2949942/1‑69 (MQ=255) tACCAGGTCTTCTACGAAGTGGCCTTCTTCATCCAAGTTGGAGTTCGCCTGGGCGATAACGTAGTTGcc > 1:3411550/1‑69 (MQ=255) tACCAGGTCTTCTACGAAGTGGCCTTCTTCATCCAAGTTGGAGTTCGCCTGGGCGATAACGTAGTTGcc > 1:556714/1‑69 (MQ=255) tACCAGGTCTTCTACGAAGTGGCCTTCTTCATCCAAGTTGGAGTTCGCCTGGGCGATAACGTAGTTGcc > 1:2630536/1‑69 (MQ=255) tACCAGGTCTTCTACGAAGTGGCCTTCTTCATCCAAGTTGGAGTTCGCCTGGGCGATAACGTAGTTGcc > 1:967176/1‑69 (MQ=255) tACCAGGTCTTCTACGAAGTGGCCTTCTTCATCCAAGTTGGAGTTCGCCTGGGCGATAACGTAGTTGc > 1:779280/1‑68 (MQ=255) ctAGGAAGTGGCCTTCTTCATCCAAGTTGGAGTTCGCCTGGGCGATAACGTAGTTGCCttcttc > 1:1201223/1‑64 (MQ=255) ctACGAAGTGGCCTTCTTCATCCAAGTTGGAGTTCGCCTGGGCGATAACGTAGTTGCCttcttc > 1:2244631/1‑64 (MQ=255) ctACGAAGTGGCCTTCTTCATCCAAGTTGGAGTTCGCCTGGGCGATAACGTAGTTGCCttcttc > 1:2938073/1‑64 (MQ=255) ctACGAAGTGGCCTTCTTCATCCAAGTTGGAGTTCGCCTGGGCGATAACGTAGTTGCCttcttc > 1:2968995/1‑64 (MQ=255) ctACGAAGTGGCCTTCTTCATCCAAGTTGGAGTTCGCCTGGGCGATAACGTAGTTGCCttcttc > 1:1955453/1‑64 (MQ=255) ctACGAAGTGGCCTTCTTCATCCAAGTTGGAGTTCGCCTGGGCGATAACGTAGTTGCCttcttc > 1:146214/1‑64 (MQ=255) ctACGAAGTGGCCTTCTTCATCCAAGTTGGAGTTCGCCTGGGCGATAACGTAGTTGCCttcttc > 1:3069378/1‑64 (MQ=255) ctACGAAGTGGCCTTCTTCATCCAAGTTGGAGTTCGCCTGGGCGATAACGTAGTTGCCttcttc > 1:934162/1‑64 (MQ=255) ctACGAAGTGGCCTTCTTCATCCAAGTTGGAGTTCGCCTGGGCGATAACGTAGTTGCCttcttc > 1:1375412/1‑64 (MQ=255) ctACGAAGTGGCCTTCTTCATCCAAGTTGGAGTTCGCCTGGGCGATAACGTAGTTGCCttcttc > 1:3330772/1‑64 (MQ=255) ctACGAAGTGGCCTTCTTCATCCAAGTTGGAGTTCGCCTGGGCGATAACGTAGTTGCCttcttc > 1:2335793/1‑64 (MQ=255) ctACGAAGTGGCCTTCTTCATCCAAGTTGGAGTTCGCCTGGGCGATAACGTAGTTGCCttcttc > 1:1627822/1‑64 (MQ=255) ctACGAAGTGGCCTTCTTCATCCAAGTTGGAGTTCGCCTGGGCGATAACGTAGTTGCCttcttc > 1:1860880/1‑64 (MQ=255) ctACGAAGTGGCCTTCTTCAGCCAAGTTGGAGTTCGCCTGGGCGATAACGTAGTTGCCttcttc > 1:3127418/1‑64 (MQ=255) tACGAAGTGGCCTTCTTCATCCAAGTTGGAGTTCGCCTGGGCGATAACGt > 1:2280215/1‑50 (MQ=255) tACGAAGTGGCCTTCTTCATCCAAGTTGGAGTTCGCCTGGGCGATAACGTAGTTGCCTTCTTCGa > 1:2608530/1‑65 (MQ=255) tACGAAGTGGCCTTCTTCATCCAAGTTGGAGTTCGCCTGGGCGATAACGTAGTTGCCTTCTTCGAt > 1:2903101/1‑66 (MQ=255) tACGAAGTGGCCTTCTTCATCCAAGTTGGAGTTCGCCTGGGCGATAACGTAGTTGCCTTCTTCGAt > 1:1448917/1‑66 (MQ=255) tACGAAGTGGCCTTCTTCATCCAAGTTGGAGTTCGCCTGGGCGATAACGTAGTTGCCTTCTTCGAt > 1:611730/1‑66 (MQ=255) tACGAAGTGGCCTTCTTCATCCAAGTTGGAGTTCGCCTGGGCGATAACGTAGTTGCCTTCTTCGAt > 1:1790143/1‑66 (MQ=255) tACGAAGTGGCCTTCTTCATCCAAGTTGGAGTTCGCCTGGGCGATAACGTAGTTGCCTTCTTCGAt > 1:2225828/1‑66 (MQ=255) tACGAAGTGGCCTTCTTCATCCAAGTTGGAGTTCGCCTGGGCGATAACGTAGTTGCCTTCTTCGAt > 1:2568700/1‑66 (MQ=255) tACGAAGTGGCATTCTTCATCCAAGTTGGAGTTCGCCTGGGCGATAACGTAGTTGCCTTCTTCGAt > 1:2585480/1‑66 (MQ=255) aCGAAGTGGCCTTCTTCATCCAGGTTGGAGTTCGCCTGGGCGATAACGTAGTTGCCTTCTTCGATa < 1:643642/66‑1 (MQ=255) aCGAAGTGGCCTTCTTCATCCAGGTTGGAGTTCGCCTGGGCGATAACGTAGTTGCCTTCTTCGATa < 1:212774/66‑1 (MQ=255) aCGAAGTGGCCTTCTTCATCCAGGTTGGAGTTCGCCTGGGCGATAACGTAGTTGCCTTCTTCGATa < 1:317660/66‑1 (MQ=255) aCGAAGTGGCCTTCTTCATCCAGGTTGGAGTTCGCCTGGGCGATAACGTAGTTGCCTTCTTCGATa < 1:3213389/66‑1 (MQ=255) aCGAAGTGGCCTTCTTCATCCAGGTTGGAGTTCGCCTGGGCGATAACGTAGTTGCCTTCTTCGATa < 1:3278828/66‑1 (MQ=255) aCGAAGTGGCCTTCTTCATCCAGGTTGGAGTTCGCCTGGGCGATAACGTAGTTGCCTTCTTCGATa < 1:2530056/66‑1 (MQ=255) aaGTGGCCTTCTTCATCCAGGTTGGAGTTCGCCTGGGCGATAACGTAGTTGCCTTCTTCGATAGCAGa < 1:652018/68‑1 (MQ=255) aaGTGGCCTTCTTCATCCAGGTTGGAGTTCGCCTGGGCGATAACGTAGTTGCCTTCTTCGATAGCAGa < 1:1055293/68‑1 (MQ=255) aaGTGGCCTTCTTCATCCAGGTTGGAGTTCGCCTGGGCGATAACGTAGTTGCCTTCTTCGATAGCAGa < 1:1705606/68‑1 (MQ=255) aaGTGGCCTTCTTCATCCAGGTTGGAGTTCGCCTGGGCGATAACGTAGTTGCCTTCTTCGATAGCAGa < 1:408772/68‑1 (MQ=255) aaGTGGCCTTCTTCATCCAGGTTGGAGTTCGCCTGGGCGATAACGTAGTTGCCTTCTTCGATAGCAGa < 1:2400692/68‑1 (MQ=255) aaGTGGCCTTCTTCATCCAGGTTGGAGTTCGCCTGGGCGATAACGTAGTTGCCTTCTTCGATAGCAGa < 1:3197338/68‑1 (MQ=255) aaGTGGCCTTCTTCATCCAGGTTGGAGTTCGCCTGGGCGATAACGTAGTTGCCTTCTTCGATAGCAGa < 1:2792900/68‑1 (MQ=255) aaGTGGCCTTCTTCATCCAGGTTGGAGTTCGCCTGGGCGATAACGTAGTTGCCTTCTTCGATAGCAGa < 1:2797949/68‑1 (MQ=255) aaGTGGCCTTCTTCATCCAAGTTGGAGTTCGCCTGGGCGATAACGTAGTTGCCTTCTTCGATAGCAGAc < 1:1573357/69‑1 (MQ=255) aGTGGCCTTCTTCATCCAAGTTGGAGTTCGCCTGGGCGATAACGTAGTTGCCTTCTTCGATAGCAGAc < 1:2022359/68‑1 (MQ=255) aGTGGCCTTCTTCATCCAAGTTGGAGTTCGCCTGGGCGATAACGTAGTTGCCTTCTTCGATAGCAGACa < 1:3415605/69‑1 (MQ=255) aGTGGCCTTCTTCATCCAAGTTGGAGTTCGCCTGGGCGATAACGTAGTTGCCTTCTTCGATAGCAGACa < 1:840419/69‑1 (MQ=255) aGTGGCCTTCTTCATCCAAGTTGGAGTTCGCCTGGGCGATAACGTAGTTGCCTTCTTCGATAGCAGACa < 1:2929988/69‑1 (MQ=255) aGTGGCCTTCTTCATCCAAGTTGGAGTTCGCCTGGGCGATAACGTAGTTGCCTTCTTCGATAGCAGACa < 1:1432637/69‑1 (MQ=255) gCCTTCTTCATCCAAGTTGGAGTTCGCCTGGGCGATAACGTAGTTGCCTTCTTCGATAGCAGACAGGTa > 1:1135223/1‑69 (MQ=255) ccTTCTTCATCCAAGTTGGAGTTCGCCTGGGCGATaa < 1:68605/37‑1 (MQ=255) ttcttcATCCAAGTTGGAGTTCGCCTGGTCGATTACGTAGTTGCCTTCTTCGATAGCAGACAGGTAGTg < 1:489003/69‑1 (MQ=255) ttcttcATCCAAGTTGGAGTTCGCCTGGGCGATAACGTAGTTGCCTTCTTCGATAGAAGACAGGTAGTg < 1:2205676/69‑1 (MQ=255) cATCCAAGTTGGAGTTCGCCTGGGCGATAACGTAGTTGCCttcttc > 1:1870798/1‑46 (MQ=255) cATCCAAGTTGGAGTTCGCCTGGGCGATAACGTAGTTGCCttcttc > 1:94879/1‑46 (MQ=255) cATCCAAGTTGGAGTTCGCCTGGGCGATAACGTAGTTGCCttcttc > 1:2440743/1‑46 (MQ=255) cATCCAAGTTGGAGTTCGCCTGGGCGATAACGTAGTTGCCttcttc > 1:1383602/1‑46 (MQ=255) cATCCAAGTTGGAGTTCGCCTGGGCGATAACGTAGTTGCCttcttc > 1:1066781/1‑46 (MQ=255) cATCCAAGTTGGAGTTCGCCTGGGCGATAACGTAGTTGCCttcttc > 1:1904598/1‑46 (MQ=255) cAAGTTGGAGTTCGCCTGGGCGATAACGTAGTTGCCTTCTTCGATAGCAGACAGGTAGTGATTTTCGTc > 1:691523/1‑69 (MQ=255) | ACAAGCTGGATTCGCCTTTGCTACGGCAAGTTACCAGGTCTTCTACGAAGTGGCCTTCTTCATCCAAGTTGGAGTTCGCCTGGGCGATAACGTAGTTGCCTTCTTCGATAGCAGACAGGTAGTGAATTTCGTC > minE/2259210‑2259342 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |