| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,260,636 | T→C | 26.7% | K169K (AAA→AAG) | rpoB ← | RNA polymerase, beta subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,260,636 | 0 | T | C | 26.7% | 89.7 / 31.8 | 82 | K169K (AAA→AAG) | rpoB | RNA polymerase, beta subunit |
| Reads supporting (aligned to +/- strand): ref base T (38/22); new base C (3/19); total (41/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.12e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TCGGATCGAATTCGAAGTCCAGCCAGGAACCACGGTAAGGGATGATACGCGCGTTATACAGCACTTTACCCGAAGAGTGGGTTTTACCTTTGTCGGAGTCAAAGAAGACGCCCGGACTAC > minE/2260572‑2260691 | tCGGATCGAATTCGAAGTCCAGCCAGGAACCACGGTAAGGGATGATACGCGCGTTATACAGCACTTTa < 1:479763/68‑1 (MQ=255) cGGATCGAATTCGAAGTCCAGCCAGGAACCACGGTAAGGGATGTTACGCGCGTTATACAGCACTTTAc > 1:2984454/1‑68 (MQ=255) cGGATCGAATTCGAAGTCCAGCCAGGAACCACGGTAAGGGATGATACGCGCGTTATACAGCACTTTa < 1:2858908/67‑1 (MQ=255) cGGATCGAATTCGAAGTCCAGCCAGGAACCACGGTAAGGGATGATACGCGCGTTATACAGCACTTTAc > 1:2363534/1‑68 (MQ=255) cGGATCGAATTCGAAGTCCAGCCAGGAACCACGGTAAGGGATGATACGCGCGTTATACAGCACTTTAc > 1:2071094/1‑68 (MQ=255) cGGATCGAATTCGAAGTCCAGCCAGGAACCACGGTAAGGGATGATACGCGCGTTATACAGCACTTTAc > 1:2010986/1‑68 (MQ=255) cGGATCGAATTCGAAGTCCAGCCAGGAACCACGGTAAGGGATGATACGCGCGTTATACAGCACTTTAc > 1:1718903/1‑68 (MQ=255) cGGATCGAATTCGAAGTCCAGCCAGGAACCACGGTAAGGGATGATACGCGCGTTATACAGCACTTTAc > 1:1416518/1‑68 (MQ=255) cGGATCGAATTCGAAGTCCAGCCAGGAACCACGGTAAGGGATGATACGCGCGTTATACAGCACTTTAc > 1:452142/1‑68 (MQ=255) cGGATCGAATTCGAAGTCCAGCCAGGAACCA‑GGTAAGGGATGATACGAGCGTTATACAGCACTTTAcc > 1:1913209/1‑68 (MQ=255) tCGAATTCGAAGTCCAGCCAGGAACCACGGTAAGGGATGATACGCGCGTTATACAGCACTTTACCCg > 1:1753979/1‑67 (MQ=255) tCGAATTCGAAGTCCAGCCAGGAACCACGGTAAGGGATGATACGCGCGTTATACAGCACTTTACCCg > 1:3080045/1‑67 (MQ=255) cGAATTCGAAGTCCAGCCAGGAACCACGGTAAGGGATGATACGCGCGTTATACAGCACTTTACCCGAag < 1:2266495/69‑1 (MQ=255) cGAATTCGAAGTCCAGCCAGGAACCACGGTAAGGGATGATACGCGCGTTATACAGCACTTTACCCGAag < 1:2036955/69‑1 (MQ=255) cGAATTCGAAGTCCAGCCAGGAACCACGGTAAGGGATGATACGCGCGTTATACAGCACTTTACCCGAag < 1:1134619/69‑1 (MQ=255) cGAATTCGAAGTCCAGCCAGGAACCACGGTAAGGGATGATACGCGCGTTATACAGCACTTTACCCGAag < 1:829252/69‑1 (MQ=255) cGAATTCGAAGTCCAGCCAGGAACCACGGTAAGGGATGATACGCGCGTTATACAGCACTTTACCCGAag < 1:1533564/69‑1 (MQ=255) cGAATTCGAAGTCCAGCCAGGAACCACGGTAAGGGATGATACGCGCGTTATACAGCACTTTACCCGAag < 1:3166657/69‑1 (MQ=255) tCCAGCCAGGAACCACGGTAAGGGATGATACGCGCGTTATACAGCACTTTACCCGAAGAGTGGGTTTTa > 1:2893758/1‑69 (MQ=255) tCCAGCCAGGAACCACGGTAAGGGATGATACGCGCGTTATACAGCACTTTACCCGAAGAGTGGGTTTTa > 1:3143648/1‑69 (MQ=255) tCCAGCCAGGAACCACGGTAAGGGATGATACGCGCGTTATACAGCACTTTACCCGAAGAGTGGGTTTTa > 1:71415/1‑69 (MQ=255) tCCAGCCAGGAACCACGGTAAGGGATGATACGCGCGTTATACAGCACTTTACCCGAAGAGTGGGTTTTa > 1:249777/1‑69 (MQ=255) tCCAGCCAGGAACCACGGTAAGGGATGATACGCGCGTTATACAGCACTTTACCCGAAGAGTGGGTTTTa > 1:2767399/1‑69 (MQ=255) cagccagGAACCACGGTAAGGGATGATGCGCGCGTTATACAGCACCTTACCCg > 1:145575/1‑53 (MQ=255) cagccagGAACCACGGTAAGGGATGATGCGCGCGTTATACAGCACCTTACCCg > 1:786393/1‑53 (MQ=255) cagccagGAACCACGGTAAGGGATGATGCGCGCGTTATACAGCACCTTACCCg > 1:2823206/1‑53 (MQ=255) cagccagGAACCACGGTAAGGGATGATACGCGCGTTATACAGCACTTTACCCGAAGAGTGGGTTTTAcc > 1:2843753/1‑69 (MQ=255) cagccagGAACCACGGTAAGGGATGATACGCGCGTTATACAGCACTTTACCCGAAGAGTGGGTTTTAcc > 1:1879736/1‑69 (MQ=255) cagccagGAACCACGGTAAGGGATGATACGCGCGTTATACAGCACTTTACCCGAAGAGTGGGTTTTAcc > 1:3240412/1‑69 (MQ=255) cagccagGAACCACGGTAAGGGATGATACGCGCGTTATACAGCACTTTACCCGAAGAGTGGGTTTTAcc > 1:1944012/1‑69 (MQ=255) cagccagGAACCACGGTAAGGGATGATACGCGCGTTATACAGCACTTTACCCGAAGAGTGGGTTTTAcc > 1:2785905/1‑69 (MQ=255) aaCCTCGGTAAGGGATGATTCGCGCGTTATACAGCACTTTACCCGAAGAGTGGGTTTTACCTTTGTCgg < 1:3341872/69‑1 (MQ=255) aaCCACGGTAAGGGATGATACGCGCGTTATACAGCACTTTACCCGAAGAGTGGGTTTTACCTTTGTCgg < 1:953074/69‑1 (MQ=255) aaCCACGGTAAGGGATGATACGCGCGTTATACAGCACTTTACCCGAAGAGTGGGTTTTACCTTTGTCgg < 1:83697/69‑1 (MQ=255) aaCCACGGTAAGGGATGATACGCGCGTTATACAGCACTTTACCCGAAGAGTGGGTTTTACCTTTGTCgg < 1:2836125/69‑1 (MQ=255) aaCCACGGTAAGGGATGATACGCGCGTTATACAGCACTTTACCCGAAGAGTGGGTTTTACCTTTGTCgg > 1:534293/1‑69 (MQ=255) aaCCACGGTAAGGGATGATACGCGCGTTATACAGCACTTTACCCGAAGAGTGGGTTTTACCTTTGTCgg < 1:2459707/69‑1 (MQ=255) aaCCACGGTAAGGGATGATACGCGCGTTATACAGCACTTTACCCGAAGAGTGGGTTTTACCTTTGTCgg < 1:1050198/69‑1 (MQ=255) aaCCACGGTAAGGGATGATACGCGCGTTATACAGCACTTTACCCGAAGAGTGGGTTTTACCTTTGTCgg > 1:1626785/1‑69 (MQ=255) aaCCACGGTAAGGGATGATACGCGCGTTATACAGCACTTTACCCGAAGAGTGGGTTTTACCTTTGTCgg < 1:2257576/69‑1 (MQ=255) cGGTAAGGGATGATACGCGCGTTATACAGCACTTTACCCGAAGAGTGGGTTTTACCTTTGTCGGAGt > 1:1428141/1‑67 (MQ=255) cGGTAAGGGATGATACGCGCGTTATACAGCACTTTACCCGAAGAGTGGGTTTTACCTTTGTCGGAGt > 1:312213/1‑67 (MQ=255) cGGTAAGGGATGATACGCGCGTTATACAGCACTTTACCCGAAGAGTGGGTTTTACCTTTGTCGGAGt > 1:308086/1‑67 (MQ=255) cGGTAAGGGATGATACGCGCGTTATACAGCACTTTACCCGAAGAGTGGGTTTTACCTTTGTCGGAGt > 1:1686972/1‑67 (MQ=255) cGGTAAGGGATGATACGCGCGTTATACAGCACTTTACCCGAAGAGTGGGTTTTACCTTTGTCGGAGt > 1:1803914/1‑67 (MQ=255) cGGTAAGGGATGATACGCGCGTTATACAGCACTTTACCCGAAGAGTGGGTTTTACCTTTGTCGGAGt > 1:2007407/1‑67 (MQ=255) cGGTAAGGGATGATACGCGCGTTATACAGCACTTTACCCAAAGAGTGGGTTTTACCTTTGTCgg < 1:1447147/64‑1 (MQ=255) cGGTAAGGGATGATACGCGCGTTATAAAGCACTTTACCCGAAGAGTGGGTTTTACCTTTGTCGGAGt > 1:2389986/1‑67 (MQ=255) atgatACGCGCGTTATACAGCACTTTACCCGAAGAGTGGGTTTTACCTTTGTCGGAGTCAAAGAAGACg < 1:1276358/69‑1 (MQ=255) atgatACGCGCGTTATACAGCACTTTACCCGAAGAGTGGGTTTTACCTTTGTCGGAGTCAAAGAAGACg < 1:1047991/69‑1 (MQ=255) atgatACGCGCGTTATACAGCACTTTACCCGAAGAGTGGGTTTTACCTTTGTCGGAGTCAAAGAAGACg < 1:1263595/69‑1 (MQ=255) atgatACGCGCGTTATACAGCACTTTACCCGAAGAGTGGGTTATACCTTTGTCGGAGTCAAAGAAGACg < 1:1694543/69‑1 (MQ=255) atACGCGCGTTATACAGCACTTTACCCGAAGAGTGGGTTTTACCTTTGTCGGAGTCAAAGAAGACGcc > 1:492847/1‑68 (MQ=255) atACGCGCGTTATACAGCACTTTACCCGAAGAGTGGGTTTTACCTTTGTCGGAGTCAAAGAAGACGcc > 1:3097242/1‑68 (MQ=255) tACGCGCGTTATACAGCACTTTACCCGAAGAGTGGGTTTTACCTTTGTCGGAGTCAAAGAAGACGCCCg > 1:2321472/1‑69 (MQ=255) aCGCGCGTTATACAGCACTTTACCCGAAGAGTGGGTTTTACCTTTGTCGGAGTCAAAGAAGACGCCCg < 1:536091/68‑1 (MQ=255) cgcgcgTTATACAGCACTTTACCCGAAGAGTGGGTTTTACCTTTGTCGGAGTCAAAGAAGACGCCCgg > 1:2168724/1‑68 (MQ=255) cgcgcgTTATACAGCACTTTACCCGAAGAGTGGGTTTTACCTTTGTCGGAGTCAAAGAAGACGCCCgg > 1:829868/1‑68 (MQ=255) cgcgcgTTATACAGCACTTTACCCGAAGAGTGGGTTTTACCTTTGTCGGAGTCAAAGAAGACGCCCgg > 1:3318697/1‑68 (MQ=255) tataCAGCACCTTACCCGAAGAGTGGGTTTTACCTTTGTc < 1:1290029/40‑1 (MQ=255) tataCAGCACCTTACCCGAAGAGTGGGTTTTACCTTTGTc < 1:227135/40‑1 (MQ=255) tataCAGCACCTTACCCGAAGAGTGGGTTTTACCTTTGTCGGa < 1:570548/43‑1 (MQ=255) tataCAGCACCTTACCCGAAGAGTGGGTTTTACCTTTGTCGGa < 1:1231644/43‑1 (MQ=255) tataCAGCACCTTACCCGAAGAGTGGGTTTTACCTTTGTCGGa < 1:1209283/43‑1 (MQ=255) tataCAGCACCTTACCCGAAGAGTGGGTTTTACCTTTGTCGGa < 1:1111288/43‑1 (MQ=255) tataCAGCACCTTACCCGAAGAGTGGGTTTTACCTTTGTCGGa < 1:948195/43‑1 (MQ=255) tataCAGCACCTTACCCGAAGAGTGGGTTTTACCTTTGTCGGa < 1:476496/43‑1 (MQ=255) tataCAGCACCTTACCCGAAGAGTGGGTTTTACCTTTGTCGGa < 1:213005/43‑1 (MQ=255) tataCAGCACCTTACCCGAAGAGTGGGTTTTACCTTTGTCGGa < 1:384846/43‑1 (MQ=255) tataCAGCACCTTACCCGAAGAGTGGGTTTTACCTTTGTCGGa < 1:1350746/43‑1 (MQ=255) tataCAGCACCTTACCCGAAGAGTGGGTTTTACCTTTGTCGGa < 1:3282639/43‑1 (MQ=255) tataCAGCACCTTACCCGAAGAGTGGGTTTTACCTTTGTCGGa < 1:1411543/43‑1 (MQ=255) tataCAGCACCTTACCCGAAGAGTGGGTTTTACCTTTGTCGGa < 1:3193621/43‑1 (MQ=255) tataCAGCACCTTACCCGAAGAGTGGGTTTTACCTTTGTCGGa < 1:2312610/43‑1 (MQ=255) tataCAGCACCTTACCCGAAGAGTGGGTTTTACCTTTGTCGGa < 1:2591580/43‑1 (MQ=255) tataCAGCACCTTACCCGAAGAGTGGGTTTTACCTTTGTCGGa < 1:1788678/43‑1 (MQ=255) tataCAGCACCTTACCCGAAGAGTGGGTTTTACCTTTGTCGGa < 1:1837464/43‑1 (MQ=255) tataCAGCACCTTACCCGAAGAGTGGGTTTTACCTTTGTCGGa < 1:2794682/43‑1 (MQ=255) gCACTTTACCCGAAGAGTGGGTTTTACCTTTGTCGGAGTCAaagaa > 1:3320199/1‑46 (MQ=255) gCACTTTACCCGAAGAGTGGGTTTTACCTTTGTCGGAGTCAaagaa > 1:796255/1‑46 (MQ=255) gCACTTTACCCGAAGAGTGGGTTTTACCTTTGTCGGAGTCAaagaa > 1:1137169/1‑46 (MQ=255) gCACTTTACCCGAAGAGTGGGTTTTACCTTTGTCGGAGTCAAAGAAGACGCCCGGACTAc < 1:2879868/60‑1 (MQ=255) | TCGGATCGAATTCGAAGTCCAGCCAGGAACCACGGTAAGGGATGATACGCGCGTTATACAGCACTTTACCCGAAGAGTGGGTTTTACCTTTGTCGGAGTCAAAGAAGACGCCCGGACTAC > minE/2260572‑2260691 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |