| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,263,269 | T→C | 44.9% | Q80Q (CAA→CAG) | rplA ← | 50S ribosomal subunit protein L1 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,263,269 | 0 | T | C | 44.9% | 14.1 / 73.9 | 89 | Q80Q (CAA→CAG) | rplA | 50S ribosomal subunit protein L1 |
| Reads supporting (aligned to +/- strand): ref base T (24/25); new base C (19/21); total (43/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.32e-01 | |||||||||||
CAGCCAGATCTTCCATACCTACCAGTTCTGCGCCTGCAGCTTTAGCAGCTTCAGCGTTTGCACCTTGGGTAAATACGGCTACGCGAACGGAACGGCCAGTACCGTGCGGCAGTACAGTTGCACCACG > minE/2263205‑2263331 | cAGCCAGATCTTCCATACCTACCAGTTCTGCGCCTGCAGCTTTAGCAGCTTCAGCGTTTGCACCTTgg > 1:3060086/1‑68 (MQ=255)cAGCCAGATCTTCCATACCTACCAGTTCTGCGCCTGCAGCTTTAGCAGCTTCAGCGTTTGCACCTTgg > 1:3228753/1‑68 (MQ=255)cAGCCAGATCTTCCATACCTACCAGTTCTGCGCCTGCAGCTTTAGCAGCTTCAGCGTTTGCACCTTgg > 1:501751/1‑68 (MQ=255)cAGCCAGATCTTCCATACCTACCAGTTCTGCGCCTGCAGCTTTAGCAGCTTCAGCGTTTGCACCTTgg > 1:1722264/1‑68 (MQ=255)cAGCCAGATCTTCCATACCTACCAGTTCTGCGCCTGCAGCTTTAGCAGCTTCAGCGTTTGCACCTTgg > 1:1642942/1‑68 (MQ=255)cAGCCAGATCTTCCATACCTACCAGTTCTGCGCCTGCAGCTTTAGCAGCTTCAGCGTTTGCACCTTgg > 1:1377808/1‑68 (MQ=255)cAGCCAGATCTTCCATACCTACCAGTTCTGCGCCTGCAGCTTTAGCAGCTTCAGCGTTTGCACCTTgg > 1:1227692/1‑68 (MQ=255)cAGCCAGATCTTCCATACCTACCAGTTCTGCGCCTGCAGCTTTAGCAGCTTCAGCGTTTGCACCTTg > 1:1050826/1‑67 (MQ=255)cAGCCAGATCTTCCATACCTACCAGTTCTGCGCCTGCAGCTTTAGCAGCTTCAGCGTTTGCACCTTg > 1:353336/1‑67 (MQ=255)cAGCCAGATCTTCCATACCTACCAGTTCTGCGCCTGCAGCTTTAGCAGCTTCAGAGTTTGCACCTTgg > 1:1090727/1‑68 (MQ=255) ccAGATCTTCCATACCTACCAGTTCTGCGCCTGCAGCTTTAGCAGCTTCAGCGTTTGCACCTTGGGTa < 1:634333/68‑1 (MQ=255) cAGATCTTCCATACCTACCAGTTCTGCGCCTGCAGCTTTAGCAGCTTCAGCGTTTGCACCTTGGGTaaa < 1:93831/69‑1 (MQ=255) cAGATCTTCCATACCTACCAGTTCTGCGCCTGCAGCTTTAGCAGCTTCAGCGTTTGCACCTTGGGTaaa < 1:556559/69‑1 (MQ=255) cAGATCTTCCATACCTACCAGTTCTGCGCCTGCAGCTTTAGCAGCTTCAGCGTTTGCACCTTGGGTaaa < 1:599145/69‑1 (MQ=255) cAGATCTTCCATACCTACCAGTTCTGCGCCTGCAGCTTTAGCAGCTTCAGCGTTTGCACCTTGGGTaaa < 1:2322458/69‑1 (MQ=255) cAGATCTTCCATACCTACCAGTTCTGCGCCTGCAGCTTTAGCAGCTTCAGCGTTTGCACCTTGGGTaaa < 1:1792361/69‑1 (MQ=255) cAGATCTTCCATACCTACCAGTTCTGCGCCTGCAGCTTTAGCAGCTTCAGCGTTTGCACCTTGGGTaaa < 1:1871894/69‑1 (MQ=255) cAGATCTTCCATACCTACCAGTTCTGCGCCTGCAGCTTTAGCAGCTTCAGCGTTTGCACCTTGGGTaaa < 1:1381561/69‑1 (MQ=255) cAGATCTTCCATACCTACCAGTTCTGCGCCTGCAGCTTTAGCAGCTTCAGCGTTTGCACCTTGGGTaaa < 1:3244365/69‑1 (MQ=255) cAGATCTTCCATACCTACCAGTTCTGCGCCTGCAGCTTTAGCAGCTTCAGCGTTTGCACCTTGGGTaaa < 1:822340/69‑1 (MQ=255) aGATCTTCCATACCTACCAGTTCTGCGCCTGCAGCTTTAGCAGCTTCAGCGTTTGCACCTTGTGTa < 1:381432/66‑1 (MQ=255) tCTTCCATACCTACCAGTTCTGCGCCTGCAGCTTTAGCAGCTTCAGCGTTTGCACCTTGGGTAAATACg > 1:3395368/1‑69 (MQ=255) ccATACCTACCAGTTCAGCACCTGCAGCTTTAGCAGCTTCAGCGTTTGCACCCTGGGTAAATACgg > 1:1954318/1‑66 (MQ=38) ccATACCTACCAGTTCAGCACCTGCAGCTTTAGCAGCTTCAGCGTTTGCACCCTGGGTAAATACgg > 1:527564/1‑66 (MQ=38) ccATACCTACCAGTTCAGCACCTGCAGCTTTAGCAGCTTCAGCGTTTGCACCCTGGGTAAATACgg > 1:1287770/1‑66 (MQ=38) ccATACCTACCAGTTCAGCACCTGCAGCTTTAGCAGCTTCAGCGTTTGCACCCTGGGTAAATACgg > 1:1974849/1‑66 (MQ=38) ccATACCTACCAGTTCAGCACCTGCAGCTTTAGCAGCTTCAGCGTTTGCACCCTGGGTAAATACgg > 1:1174630/1‑66 (MQ=38) ccATACCTACCAGTTCAGCACCTGCAGCTTTAGCAGCTTCAGCGTTTGCACCCTGGGTAAATACgg > 1:2326567/1‑66 (MQ=38) ccATACCTACCAGTTCAGCACCTGCAGCTTTAGCAGCTTCAGCGTTTGCACCCTGGGTAAATACgg > 1:2381610/1‑66 (MQ=38) ccATACCTACCAGTTCAGCACCTGCAGCTTTAGCAGCTTCAGCGTTTGCACCCTGGGTAAATACgg > 1:2518060/1‑66 (MQ=38) ccATACCTACCAGTTCAGCACCTGCAGCTTTAGCAGCTTCAGCGTTTGCACCCTGGGTAAATACgg > 1:351930/1‑66 (MQ=38) ccATACCTACCAGTTCAGCACCTGCAGCTTTAGCAGCTTCAGCGTTTGCACCCTGGGTAAATACgg > 1:3351404/1‑66 (MQ=38) ccATACCTACCAGTTCAGCACCTGCAGCTTTAGCAGCTTCAGCGTTTGCACCCTGGGTAAATACgg > 1:1947676/1‑66 (MQ=38) ccATACCTACCAGTTCAGCACCTGCAGCTTTAGCAGCTTCAGCGTTTGCACCCTGGGTAAATACgg > 1:1341713/1‑66 (MQ=38) ccATACCTACCAGTTCAGCACCTGCAGCTTTAGCAGCTTCAGCGTTTGCACCCTGGGTAAATACgg > 1:822361/1‑66 (MQ=38) ccATACCTACCAGTTCAGCACCTGCAGCTTTAGCAGCTTCAGCGTTTGCACCCTGGGTAAATACgg > 1:1406386/1‑66 (MQ=38) ccATACCTACCAGTTCAGCACCTGCAGCTTTAGCAGCTTCAGCGTTTGCACCCTGGGTAAATACgg > 1:819446/1‑66 (MQ=38) ccATACCTACCAGTTCAGCACCTGCAGCTTTAGCAGCTTCAGCGTTTGCACCCTGGGTAAATACgg > 1:782693/1‑66 (MQ=38) ccATACCTACCAGTTCAGCACCTGCAGCTTTAGCAGCTTCAGCGTTTGCACCCTGGGTAAATACgg > 1:1510994/1‑66 (MQ=38) ccATACCTACCAGTTCAGCACCTGCAGCTTTAGCAGCTTCAGCGTTTGCACCCTGGGTAAATACgg > 1:1197461/1‑66 (MQ=38) ccATACATACCAGTTCAGCACCTGCAGCTTTAGCAGCTTCAGCGTTTGCACCCTGGGTAAATACgg > 1:4728/1‑66 (MQ=37) cctaccAGTTCTGCGCCTGCAGCTTTAGCAGCTTCAGCGTTTGCACCTTGGGTAAATACGGCTACGCga > 1:2338463/1‑69 (MQ=255) cctaccAGTTCTGCGCCTGCAGCTTTAGCAGCTTCAGCGTTTGCACCTTGGGTAAATACGGCTACGCga > 1:235917/1‑69 (MQ=255) cctaccAGTTCTGCGCCTGCAGCTTTAGCAGCTTCAGCGTTTGCACCTTGGGTAAATACGGCTACGCga > 1:3020236/1‑69 (MQ=255) cctaccAGTTCTGCGCCTGCAGCTTTAGCAGCTTCAGCGTTTGCACCTTGGGTAAATACGGCTACGCga > 1:2417953/1‑69 (MQ=255) cctaccAGTTCTGCGCCTGCAGCTTTAGCAGCTTCAGCGTTTGCACCTTGGGTAAATACGGCTACGCga > 1:2970115/1‑69 (MQ=255) cctaccAGTTCTGCGCCTGCAGCTTTAGCAGCTTCAGCGTTTGCACCTTGGGTAAATACGGCTACGCga > 1:1029714/1‑69 (MQ=255) cctaccAGTTCTGCGCCTGCAGCTTTAGCAGCTTCAGCGTTTGCACCTTGGGTAAATACGGCTACGCga > 1:1639735/1‑69 (MQ=255) cctaccAGTTCTGCGCCTGCAGCTTTAGCAGCTTCAGCGTTTGCACCTTGGGTAAATACGGCTACGCga > 1:1699078/1‑69 (MQ=255) cctaccAGTTCTGCGCCTGCAGCTTTAGCAGCTTCAGCGTTTGCACCTTGGGTAAATACGGCTACGCga > 1:2589711/1‑69 (MQ=255) cctaccAGTTCTGCGCCTGCAGCTTTAGCAGCTTCAGCGTTTGCACCTTGGGTAAATACGGCTACGCga > 1:321082/1‑69 (MQ=255) cctaccAGTTCTGCGCCTGCAGCTTTAGCAGCTTCAGCGTTTGCACCTTGGGTAAATACGGCTACGCga > 1:1790761/1‑69 (MQ=255) cctaccAGTTCTGCGCCTGCAGCTTTAGCAGCTTCAGCGTTTGCACCTTGGGTAAATACGGCTACGCga > 1:1898423/1‑69 (MQ=255) aGTTCTGCGCCTGCAGCTTTAGCAGCTTCAGCGTTTGCACCTTGGGTAAATACGGCTACGCgaacgg < 1:193650/67‑1 (MQ=255) aGTTCTGCGCCTGCAGCTTTAGCAGCTTCAGCGTTTGCACCTTGGGTAAATACGGCTACGCgaacgg < 1:2730652/67‑1 (MQ=255) aGTTCTGCGCCTGCAGCTTTAGCAGCTTCAGCGTTTGCACCTTGGGTAAATACGGCTACGCgaacgg < 1:1206960/67‑1 (MQ=255) aGTTCTGCGCCTGCAGCTTTAGCAGCTTCAGCGTTTGCACCTTGGGTAAATACGGCTACGCgaacgg < 1:1667336/67‑1 (MQ=255) gcgcCTGCAGCTTTAGCAGCTTCAGCGTTTGCACCTTGGGTAAATACGGCTACGCGAACGGAACGGcc < 1:2482362/68‑1 (MQ=255) gcgcCTGCAGCTTTAGCAGCTTCAGCGTTTGCACCTTGGGTAAATACGGCTACGCGAACGGAACGGcc < 1:1321310/68‑1 (MQ=255) gcgcCTGCAGCTTTAGCAGCTTCAGCGTTTGCACCTTGGGTAAATACGGCTACGCGAACGGAACGGcc < 1:2208872/68‑1 (MQ=255) tCAGCGTTTGCACCCTGGGTAAATACGGCTACGCGAACGGAACGGCCAGTACCGTGCGGCAGTACAGtt < 1:3114096/69‑1 (MQ=255) tCAGCGTTTGCACCCTGGGTAAATACGGCTACGCGAACGGAACGGCCAGTACCGTGCGGCAGTACAGtt < 1:1170789/69‑1 (MQ=255) tCAGCGTTTGCACCCTGGGTAAATACGGCTACGCGAACGGAACGGCCAGTACCGTGCGGCAGTACAGtt < 1:829836/69‑1 (MQ=255) tCAGCGTTTGCACCCTGGGTAAATACGGCTACGCGAACGGAACGGCCAGTACCGTGCGGCAGTACAGtt < 1:649385/69‑1 (MQ=255) tCAGCGTTTGCACCCTGGGTAAATACGGCTACGCGAACGGAACGGCCAGTACCGTGCGGCAGTACAGtt < 1:16967/69‑1 (MQ=255) tCAGCGTTTGCACCCTGGGTAAATACGGCTACGCGAACGGAACGGCCAGTACCGTGCGGCAGTACAGtt < 1:1740841/69‑1 (MQ=255) tCAGCGTTTGCACCCTGGGTAAATACGGCTACGCGAACGGAACGGCCAGTACCGTGCGGCAGTACAGtt < 1:207667/69‑1 (MQ=255) tCAGCGTTTGCACCCTGGGTAAATACGGCTACGCGAACGGAACGGCCAGTACCGTGCGGCAGTACAGtt < 1:513702/69‑1 (MQ=255) tCAGCGTTTGCACCCTGGGTAAATACGGCTACGCGAACGGAACGGCCAGTACCGTGCGGCAGTACAGtt < 1:2189332/69‑1 (MQ=255) tCAGCGTTTGCACCCTGGGTAAATACGGCTACGCGAACGGAACGGCCAGTACCGTGCGGCAGTACAGtt < 1:432679/69‑1 (MQ=255) tCAGCGTTTGCACCCTGGGTAAATACGGCTACGCGAACGGAACGGCCAGTACCGTGCGGCAGTACAGtt < 1:39614/69‑1 (MQ=255) tCAGCGTTTGCACCCTGGGTAAATACGGCTACGCGAACGGAACGGCCAGTACCGTGCGGCAGTACAGtt < 1:2669841/69‑1 (MQ=255) tCAGCGTTTGCACCCTGGGTAAATACGGCTACGCGAACGGAACGGCCAGTACCGTGCGGCAGTACAGtt < 1:2869215/69‑1 (MQ=255) tCAGCGTTTGCACCCTGGGTAAATACGGCTACGCGAACGGAACGGCCAGTACCGTGCGGCAGTACAGtt < 1:2877345/69‑1 (MQ=255) tCAGCGTTTGCACCCTGGGTAAATACGGCTACGCGAACGGAACGGCCAGTACCGTGCGGCAGTACAGtt < 1:3345973/69‑1 (MQ=255) tCAGCGTTTGCACCCTGGGTAAATACGGCTACGCGAACGGAACGGCCAGTACCGTGCGGCAGTACAGtt < 1:3066005/69‑1 (MQ=255) tCAGCGTTTGCACCCTGGGTAAATACGGCTACGCGAACGGAACGGCCAGTACCGTGCGGCAGTACAGtt < 1:3187385/69‑1 (MQ=255) tCAGCGTTTGCACCCTGGGTAAATACGGCTACGCGAACGGAACGGCCAGTACCGTGCGGCAGTACAGtt < 1:3264117/69‑1 (MQ=255) tCAGCGTTTGCACCCTGGGTAAATACGGCTACGCGAACGGAACGGCCAGTACCGTGCGGCAGTACAGtt < 1:2906126/69‑1 (MQ=255) tCAGCGTTTGCACCCTGGGTAAATACGGCTACGCGAACGGAACGGCCAGTACCGTGCGGCAGTACAGtt < 1:2924595/69‑1 (MQ=255) tCAGCGTTTGCACCCTGGGTAAATACGGCTACGCGAACGGAACGGCCAGCACCGTGCGGCAGTACAGtt < 1:2472345/69‑1 (MQ=255) gCGTTTGCACCTTGGGTAAATACGGCTACGCGAACGGAACGGCCAGTACCGTGCGGCAGTACAGTTg < 1:1496767/67‑1 (MQ=255) tttGCACCTTGGGTAAATACGGCTACGCGAACGGAACGGCCAGTACCGTGCGGCAGTACAGTTGcacca < 1:2899949/69‑1 (MQ=255) tttGCACCTTGGGTAAATACGGCTACGCGAACGGAACGGCCAGTACCGTGCGGCAGTACAGTTGcacca < 1:972567/69‑1 (MQ=255) tttGCACCTTGGGTAAATACGGCTACGCGAACGGAACGGCCAGTACCGTGCGGCAGTACAGTTGcacca < 1:1211087/69‑1 (MQ=255) tttGCACCTTGGGTAAATACGGCTACGCGAACGGAACGGCCAGTACCGTGCGGCAGTACAGTTGcacca < 1:556595/69‑1 (MQ=255) tttGCACCTTGGGTAAATACGGCTACGCGAACGGAACGGCCAGTACCGTGCGGCAGTACAGTTGcacca < 1:2579434/69‑1 (MQ=255) ttGCACCTTGGGTAAATACGGCTACGCGAACGGAACGGCCAGTACCGTGCGGCAGTACAGTTGcacca < 1:1472681/68‑1 (MQ=255) tGCACCTTGGGTAAATACGGCTACGCGAACGGAACGGCCAGTACAGTGCGGCAGTACAGTTGCACCacg > 1:1768427/1‑69 (MQ=255) | CAGCCAGATCTTCCATACCTACCAGTTCTGCGCCTGCAGCTTTAGCAGCTTCAGCGTTTGCACCTTGGGTAAATACGGCTACGCGAACGGAACGGCCAGTACCGTGCGGCAGTACAGTTGCACCACG > minE/2263205‑2263331 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |