| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,269,128 | G→A | 24.6% | L60L (CTG→TTG) | birA ← | bifunctional biotin‑[acetylCoA carboxylase] holoenzyme synthetase and DNA‑binding transcriptional repressor, bio‑5'‑AMP‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,269,128 | 0 | G | A | 24.6% | 139.9 / 37.3 | 109 | L60L (CTG→TTG) | birA | bifunctional biotin‑[acetylCoA carboxylase] holoenzyme synthetase and DNA‑binding transcriptional repressor, bio‑5'‑AMP‑binding |
| Reads supporting (aligned to +/- strand): ref base G (44/38); new base A (21/6); total (65/44) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.07e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.13e-01 | |||||||||||
Only 100 of 112 total aligned reads displayed.
ACACTACCGCCATCCAGCTGACCCAATATCTGTTTAGCATTAAGTAACTGGATAGGCTCAGGCAGGCTGTATCCTTTACCCGGAACGGTAAAGACATCAACGCCCCAGTCACGCAGTGTCTGAATGTGTT > minE/2269064‑2269193 | acacTACCGCCATCCAGCTGACCCAATATCTGTTTAGCATTAAGTAACTGGATAGGCTCAGGCAGGCTg < 1:106467/69‑1 (MQ=255)acacTACCGCCATCCAGCTGACCCAATATCTGTTTAGCATTAAGTAACTGGATAGGCTCAGGCAGGCTg < 1:3200387/69‑1 (MQ=255)acacTACCGCCATCCAGCTGACCCAATATCTGTTTAGCATTAAGTAACTGGATAGGCTCAGGCAGGCTg < 1:764333/69‑1 (MQ=255)acacTACCGCCATCCAGCTGACCCAATATCTGTTTAGCATTAAGTAACTGGATAGGCTCAGGCAGGCTg < 1:50261/69‑1 (MQ=255)acacTACCGCCATCCAGCTGACCCAATATCTGTTTAGCATTAAGTAACTGGATAGGCTCAGGCAGGCTg < 1:3097860/69‑1 (MQ=255)acacTACCGCCATCCAGCTGACCCAATATCTGTTTAGCATTAAGTAACTGGATAGGCTCAGGCAGGCTg < 1:1241138/69‑1 (MQ=255)acacTACCGCCATCCAGCTGACCCAATATCTGTTTAGCATTAAGTAACTGGATAGGCTCAGGCAGGCTg < 1:1266194/69‑1 (MQ=255) cTACCGCCATCCAGCTGACCCAATATCTGTTTAGCATTAAGTAACTGGATAGGCTCAGGCAGGCTGTAt > 1:207912/1‑69 (MQ=255) cTACCGCCATCCAGCTGACCCAATATCTGTTTAGCATTAAGTAACTGGATAGGCTCAGGCAGGCTGTAt > 1:1911981/1‑69 (MQ=255) cTACCGCCATCCAGCTGACCCAATATCTGTTTAGCATTAAGTAACTGGATAGGCTCAGGCAGGCTGTAt > 1:3245272/1‑69 (MQ=255) aCCGCCATCCAGCTGACCCAATATCTGTTTAGCATTAAGTAACTGGATAGGCTCAGGCAGGCTGTATcc > 1:2880623/1‑69 (MQ=255) aCCGCCATCCAGCTGACCCAATATCTGTTTAGCATTAAGTAACTGGATAGGCTCAGGCAGGCTGTATcc > 1:549349/1‑69 (MQ=255) aCCGCCATCCAGCTGACCCAATATCTGTTTAGCATTAAGTAACTGGATAGGCTCAGGCAGGCTGTATcc > 1:1281618/1‑69 (MQ=255) aCCGCCATCCAGCTGACCCAATATCTGTTTAGCATTAAGTAACTGGATAGGCTCAGGCAGGCTGTATcc > 1:2616405/1‑69 (MQ=255) aCCGCCATCCAGCTGACCCAATATCTGTTTAGCATTAAGTAACTGGATAGGCTCAGGCAGGCTGTATcc > 1:2750272/1‑69 (MQ=255) ccAGCTGACCCAATATCTGTTTAGCATTAAGTAACTGGATAGGCTCAGGCAGGCTg < 1:1321519/56‑1 (MQ=255) cAGCTGACCCAATATCTGTTTAGCATTAAGTAACTGGATAGGCTCAGGCAGGCTGTATCCTTTACCCgg < 1:1223618/69‑1 (MQ=255) cAGCTGACCCAATATCTGTTTAGCATTAAGTAACTGGATAGGCTCAGGCAGGCTGTATCCTTTACCCgg < 1:1171572/69‑1 (MQ=255) cAGCTGACCCAATATCTGTTTAGCATTAAGTAACTGGATAGGCTCAGGCAGGCTGTATCCTTTACCCgg < 1:2076700/69‑1 (MQ=255) cAGCTGACCCAATATCTGTTTAGCATTAAGTAACTGGATAGGCTCAGGCAGGCTGTATCCTTTACCCgg < 1:2129430/69‑1 (MQ=255) cAGCTGACCCAATATCTGTTTAGCATTAAGTAACTGGATAGGCTCAGGCAGGCTGTATCCTTTACCCgg < 1:533760/69‑1 (MQ=255) aaTATCTTTTCGGCATTAAGTAACTGGATTGGCTCGGGCAAGCTGTATCCTTTa > 1:1463163/1‑54 (MQ=255) aaTATCTTTTCGGCATTAAGTAACTGGATTGGCTCGGGCAAGCTGTATCCTTTACCCGGAACAGTaa > 1:1845793/1‑67 (MQ=255) aaTATCTTTTCGGCATTAAGTAACTGGATTGGCTCGGGCAAGCTGTATCCTTTACCCGGAACAGTaa > 1:428389/1‑67 (MQ=255) aaTATCTTTTCGGCATTAAGTAACTGGATTGGCTCGGGCAAGCTGTATCCTTTACCCGGAACAGTaa > 1:2420829/1‑67 (MQ=255) aaTATCTTTTCGGCATTAAGTAACTGGATTGGCTCGGGCAAGCTGTATCCTTTACCCGGAACAGTaa > 1:2517044/1‑67 (MQ=255) aaTATCTTTTCGGCATTAAGTAACTGGATTGGCTCGGGCAAGCTGTATCCTTTACCCGGAACAGTaa > 1:2656005/1‑67 (MQ=255) aaTATCTTTTCGGCATTAAGTAACTGGATTGGCTCGGGCAAGCTGTATCCTTTACCCGGAACAGTaa > 1:299926/1‑67 (MQ=255) aaTATCTTTTCGGCATTAAGTAACTGGATTGGCTCGGGCAAGCTGTATCCTTTACCCGGAACAGTaa > 1:2959785/1‑67 (MQ=255) aaTATCTTTTCGGCATTAAGTAACTGGATTGGCTCGGGCAAGCTGTATCCTTTACCCGGAACAGTaa > 1:736102/1‑67 (MQ=255) aaTATCTTTTCGGCATTAAGTAACTGGATTGGCTCGGGCAAGCTGTATCCTTTACCCGGAACAGTaa > 1:1036552/1‑67 (MQ=255) aaTATCTTTTCGGCATTAAGTAACTGGATTGGCTCGGGCAAGCTGTATCCTTTACCCGGAACAGTaa > 1:1178682/1‑67 (MQ=255) aaTATCTTTTCGGCATTAAGTAACTGGATTGGCTCGGGCAAGCTGTATCCTTTACCCGGAACAGTaa > 1:1452602/1‑67 (MQ=255) aaTATCTTTTCGGCATTAAGTAACTGGATTGGCTCGGGCAAGCTGTATCCTTTACCCGGAACAGTaa > 1:528529/1‑67 (MQ=255) aaTATCTTTTCGGCATTAAGTAACTGGATTGGCTCGGGCAAGCTGTATCCTTTACCCGGAACAGTaa > 1:1739309/1‑67 (MQ=255) aaTATCTTTTCGGCATTAAGTAACTGGATTGGCTCGGGCAAGCTGTATCCTTTACCCGGAACAGTa > 1:2010183/1‑66 (MQ=255) aaTATCTTTTCGGCATTAAGTAACTGGATTGGCTCGGGCAAGCTGTATCCTTTACCCGGAACAGTa > 1:846025/1‑66 (MQ=255) aaTATCTTTTCGGCATTAAGTAACTGGATTGGCTCGGGCAAGCTGTATCCTTTACCCGGAACAGTa > 1:2996610/1‑66 (MQ=255) aaTATCTTTTCGGCATTAAGTAACTGGATTGGCTCGGGCAAGCTGTATCCTTTACCCGGAACAGTa > 1:3072797/1‑66 (MQ=255) aaTATCTTTTCGGCATTAAGTAACTGGATTGGCTCGGGCAAGCTGTATCCTTTACCCGGAACAGTa > 1:807507/1‑66 (MQ=255) tCTGTTTAGCATTAAGTAACTGGATAGGCTCAGGCAGTCTGTATCCTTTACCCGGAACGGTAAAGa > 1:1156613/1‑66 (MQ=255) tCTGTTTAGCATTAAGTAACTGGATAGGCTCAGGCAGGCTTTATCCTTTACCAGGAAc > 1:845339/1‑58 (MQ=255) tCTGTTTAGCATTAAGTAACTGGATAGGCTCAGGCAGGCTGTATCCTTTACCCGGAACGGTAAAGa > 1:2300763/1‑66 (MQ=255) tCTGTTTAGCATTAAGTAACTGGATAGGCTCAGGCAGGCTGTATCCTTTACCCGGAACGGTAAAGa > 1:711975/1‑66 (MQ=255) tCTGTTTAGCATTAAGTAACTGGATAGGCTCAGGCAGGCTGTATCCTTTACCCGGAACGGTAAAGa > 1:3385530/1‑66 (MQ=255) tCTGTTTAGCATTAAGTAACTGGATAGGCTCAGGCAGGCTGTATCCTTTACCCGGAACGGTAAAGa > 1:3171324/1‑66 (MQ=255) tCTGTTTAGCATTAAGTAACTGGATAGGCTCAGGCAGGCTGTATCCTTTACCCGGAACGGTAAAGa > 1:120788/1‑66 (MQ=255) tCTGTTTAGCATTAAGTAACTGGATAGGCTCAGGCAGGCTGTATCCTTTACCCGGAACGGTAAAGa > 1:1738614/1‑66 (MQ=255) tCTGTTTAGCATTAAGTAACTGGATAGGCTCAGGCAGGCTGTATCCTTTACCCGGAACGGTAAAGa > 1:304790/1‑66 (MQ=255) tCTGTTTAGCATTAAGTAACTGGATAGGCTCAGGCAGGCTGTATCCTTTACCCGGAACGGTAAAGa > 1:1803552/1‑66 (MQ=255) tCTGTTTAGCATTAAGTAACTGGATAGGCTCAGGCAGGCTGTATCCTTTACCCGGAACGGTAAAGa > 1:1126352/1‑66 (MQ=255) tCTGTTTAGCATTAAGTAACTGGATAGGCTCAGGCAGGCTGTATCCTTTACCCGGAACGGTAAAGACa > 1:2760100/1‑68 (MQ=255) tCTGTTTAGCATTAAGTAACTGGATAGGCTCAGGCAGGCTGTATCCTTTACCCGGAACGGTAAAGACa > 1:295026/1‑68 (MQ=255) tCTGTTTAGCATTAAGTAACTGGATAGGCTCAGGCAGGCTGTATCCTTTACCCGGAACGGTAAAGACa > 1:3352118/1‑68 (MQ=255) cTGTTTAGCATTAAGTAACTGGATAGGCTCAGGCAGGCTGTATCCTTTACCCGGAACGGTAAAGACATc > 1:188621/1‑69 (MQ=255) cTGTTTAGCATTAAGTAACTGGATAGGCTCAGGCAGGCTGTATCCTTTACCCGGAACGGTAAAGACATc > 1:3210307/1‑69 (MQ=255) tGTTTAGCATTAAGTAACTGGATAGGCTCAGGCAGGCTGTATCCTTTACCCGGAACGGTAAAGa < 1:416781/64‑1 (MQ=255) tGTTTAGCATTAAGTAACTGGATAGGCTCAGGCAGGCTGTATCCTTTACCCGGAACGGTAAAGa < 1:2642416/64‑1 (MQ=255) tGTTTAGCATTAAGTAACTGGATAGGCTCAGGCAGGCTGTATCCTTTACCCGGAACGGTAAAGa < 1:668437/64‑1 (MQ=255) tGTTTAGCATTAAGTAACTGGATAGGCTCAGGCAGGCTGTATCCTTTACCCGGAACGGTAAAGa < 1:1356086/64‑1 (MQ=255) tGTTTAGCATTAAGTAACTGGATAGGCTCAGGCAGGCTGTATCCTTTACCCGGAACGGTAAAGa < 1:1536519/64‑1 (MQ=255) tGTTTAGCATTAAGTAACTGGATAGGCTCAGGCAGGCTGTATCCTTTACCCGGAACGGTAAAGa < 1:1528698/64‑1 (MQ=255) tGTTTAGCATTAAGTAACTGGATAGGCTCAGGCAGGCTGTATCCTTTACCCGGAACGGTAAAGACATc < 1:1911044/68‑1 (MQ=255) tAGCATTAAGTAACTGGATAGGCTCAGGCAGGCTGTATCCTTTACCCGGAACGGTAAAGa > 1:2008346/1‑60 (MQ=255) tAGCATTAAGTAACTGGATAGGCTCAGGCAGGCTGTATCCTTTACCCGGAACGGTAAAGACATCAACGc > 1:2064127/1‑69 (MQ=255) gCATTAAGTAACTGGATAGGCTCAGGCAGGCTGTATCCTTTACCCGGAACGGTAAAGACATCAACGcc < 1:1971956/68‑1 (MQ=255) gCATTAAGTAACTGGATAGGCTCAGGCAGGCTGTATCCTTTACCCGGAACGGTAAAGACATCAACGcc < 1:3328072/68‑1 (MQ=255) gCATTAAGTAACTGGATAGGCTCAGGCAGGCTGTATCCTTTACCCGGAACGGTAAAGACATCAACGcc < 1:1402068/68‑1 (MQ=255) gCATTAAGTAACTGGATAGGCTCAGGCAGGCTGTATACTTTACCCGGAACGGTAAAGACATCAACGcc < 1:3203999/68‑1 (MQ=255) ttAAGTAACTGGATAGGCTCAGGCAGGCTGTATCCTTTACCCGGAACGGTa > 1:2733955/1‑51 (MQ=255) ttAAGTAACTGGATAGGCTCAGGCAGGCTGTATCCTTTACCCGGAACGGTAAAGACATCAACGCCCCa > 1:1029377/1‑68 (MQ=255) aaGTAACTGGATAGGCTCAGGCAGGCTGTATCCTTTACCCGGAACGGTAAAGACATCAACGCCCCAGTc > 1:1504353/1‑69 (MQ=255) aaGTAACTGGATAGGCTCAGGCAGGCTGTATCCTTTACCCGGAACGGTAAAGACATCAACGCCCCAGTc > 1:1727879/1‑69 (MQ=255) aaGTAACTGGATAGGCTCAGGCAGGCTGTATCCTTTACCCGGAACGGTAAAGACATCAACGCCCCAGTc > 1:1202521/1‑69 (MQ=255) tAACTGGATTGGCTCGGGCAAGCTGTATCCTTTACCCGGAACAGTAAATACATCAACGCCCCAGTCAc < 1:67164/68‑1 (MQ=255) tAACTGGATTGGCTCGGGCAAGCTGTATCCTTTACCCGGAACAGTAAATACATCAACGCCCCAGTCAc < 1:897581/68‑1 (MQ=255) tAACTGGATTGGCTCGGGCAAGCTGTATCCTTTACCCGGAACAGTAAATACATCAACGCCCCAGTCAc < 1:905108/68‑1 (MQ=255) tAACTGGATTGGCTCGGGCAAGCTGTATCCTTTACCCGGAACAGTAAATACATCAACGCCCCAGTCAc < 1:2955934/68‑1 (MQ=255) tAACTGGATTGGCTCGGGCAAGCTGTATCCTTTACCCGGAACAGTAAATACATCAACGCCCCAGTCAc < 1:2702973/68‑1 (MQ=255) tAACTGGATTGGCTCGGGCAAGCTGTATCCTTTACCCGGAACAGTAAATACATCAACGCCCCAGTCAc < 1:2450390/68‑1 (MQ=255) ggATAGGCTCAGGCAGGCTTTATCCTTTACCCGGAACGGTAAAGACATCAACGCCCCAGTCACGCAgtg < 1:2620037/69‑1 (MQ=255) ggATAGGCTCAGGCAGGCTGTATCCTTTACCCGGAACGGTAAAGACAt > 1:1318542/1‑48 (MQ=255) ggATAGGCTCAGGCAGGCTGTATCCTTTACCCGGAACGGTAAAGACATc > 1:335700/1‑49 (MQ=255) ggATAGGCTCAGGCAGGCTGTATCCTTTACCCGGAACGGTAAAGACATc > 1:889826/1‑49 (MQ=255) ggATAGGCTCAGGCAGGCTGTATCCTTTACCCGGAACGGTAAAGACATc > 1:464187/1‑49 (MQ=255) ggATAGGCTCAGGCAGGCTGTATCCTTTACCCGGAACGGTAAAGACATc > 1:2153685/1‑49 (MQ=255) ggATAGGCTCAGGCAGGCTGTATCCTTTACCCGGAACGGTAAAGACATc > 1:2566918/1‑49 (MQ=255) ggATAGGCTCAGGCAGGCTGTATCCTTTACCCGGAACGGTAAAGACATc > 1:3043532/1‑49 (MQ=255) ggATAGGCTCAGGCAGGCTGTATCCTTTACCCGGAACGGTAAAGACATCAACGCCCCAGTCACGCAgtg < 1:2727903/69‑1 (MQ=255) ggATAGGCTCAGGCAGGCTGTATCCTTTACCCGGAACGGTAAAGACATCAACGCCCCAGTCACGCAgtg < 1:3013579/69‑1 (MQ=255) ggATAGGCTCAGGCAGGCTGTATCCTTTACCCGGAACGGTAAAGACATCAACGCCCCAGTCACGCAgtg < 1:1363424/69‑1 (MQ=255) ggATAGGCTCAGGCAGGCTGTATCCTTTACCCGGAACGGTAAAGACATCAACGCCCCAGTCACGCAgtg < 1:2024964/69‑1 (MQ=255) gATAGGCTCAGGCAGGCTGTATCCTTTACCCGGAACGGTAAAGACATCAACGCCCCAGTCACGCAgtg < 1:899145/68‑1 (MQ=255) cTCAGGCAGGCTGTATCCTTTACCCGGAACGGTAAAGACATCAACGCCCCAGTCACGCAGTGTCTGaa < 1:3252024/68‑1 (MQ=255) cTCAGGCAGGCTGTATCCTTTACCCGGAACGGTAAAGACATCAACGCCCCAGTCACGCAGTGTCTGaa < 1:2669189/68‑1 (MQ=255) cTCAGGCAGGCTGTATCCTTTACCCGGAACGGTAAAGACATCAACGCCCCAGTCACGCAGTGTCTGaa < 1:1125677/68‑1 (MQ=255) cTCAGGCAGGCTGTATCCTTTACCCGGAACGGTAAAGACATCAACGCCCCAGTCACGCAGTGTCTGaa < 1:3400513/68‑1 (MQ=255) gcaggcTGTATCCTTTACCCGGAACGGTAAAGACATCAACGCCCCAGTCACGCAGTGTCTGAATGTGtt < 1:1424113/69‑1 (MQ=255) gcaggcTGTATCCTTTACCCGGAACGGTAAAGACATCAACGCCCCAGTCACGCAGTGTCTGAATGTGtt < 1:2334599/69‑1 (MQ=255) caggcTGTATCCTTTACCCGGAACGGTAAAGACATCAACGCCCCAGTCACGCAGTGTCTGAAtgtg < 1:3404009/66‑1 (MQ=255) | ACACTACCGCCATCCAGCTGACCCAATATCTGTTTAGCATTAAGTAACTGGATAGGCTCAGGCAGGCTGTATCCTTTACCCGGAACGGTAAAGACATCAACGCCCCAGTCACGCAGTGTCTGAATGTGTT > minE/2269064‑2269193 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |