| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 190,273 | A→G | 34.9% | A590A (GCA→GCG) | ldcC → | lysine decarboxylase 2, constitutive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 190,273 | 0 | A | G | 34.9% | 85.9 / 75.9 | 126 | A590A (GCA→GCG) | ldcC | lysine decarboxylase 2, constitutive |
| Reads supporting (aligned to +/- strand): ref base A (52/30); new base G (17/27); total (69/57) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.05e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01 | |||||||||||
Only 100 of 126 total aligned reads displayed.
ATGCTACCCGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTC > minE/190208‑190333 | atGCTACCCGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACaa > 1:400939/1‑69 (MQ=255)atGCTACCCGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACaa > 1:464870/1‑69 (MQ=255)atGCTACCCGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACaa < 1:1518737/69‑1 (MQ=255) tGCTACCCGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACaa < 1:1833731/68‑1 (MQ=255) cTACCCGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAggg < 1:344972/69‑1 (MQ=255) cTACCCGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAggg < 1:2677615/69‑1 (MQ=255) cTACCCGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAggg < 1:1118247/69‑1 (MQ=255) cTACCCGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAggg < 1:2570377/69‑1 (MQ=255) cTACCCGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAggg < 1:2495121/69‑1 (MQ=255) tACCCGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAggg < 1:859711/68‑1 (MQ=255) ctctATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAg > 1:1584889/1‑69 (MQ=255) ctctATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAg > 1:1423621/1‑69 (MQ=255) ctATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCt > 1:2760614/1‑69 (MQ=255) ctATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCt > 1:1847208/1‑69 (MQ=255) ctATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCt > 1:3237454/1‑69 (MQ=255) ctATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCt > 1:3036684/1‑69 (MQ=255) ctATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCt > 1:1446452/1‑69 (MQ=255) ctATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCt > 1:2992233/1‑69 (MQ=255) ctATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCt > 1:1068758/1‑69 (MQ=255) tGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCt < 1:3389400/66‑1 (MQ=255) tGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCt < 1:2676414/66‑1 (MQ=255) tGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCt < 1:2592164/66‑1 (MQ=255) tGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCt < 1:1152762/66‑1 (MQ=255) ccGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAacac > 1:3430005/1‑68 (MQ=255) ccGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAacac > 1:773085/1‑68 (MQ=255) ccGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACg > 1:2340818/1‑69 (MQ=255) ttCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCtt > 1:2269702/1‑69 (MQ=255) ttCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCtt > 1:2129342/1‑69 (MQ=255) cAATATGCGTATTCAGGATCTGGCGCAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGttt > 1:2671164/1‑69 (MQ=255) cAATATGCGTATTCAGGATCTGGCGCAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGttt > 1:2580735/1‑69 (MQ=255) cAATATGCGTATTCAGGATCTGGCGCAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGttt > 1:2372515/1‑69 (MQ=255) cAATATGCGTATTCAGGATCTGGCGCAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGttt > 1:2374597/1‑69 (MQ=255) cAATATGCGTATTCAGGATCTGGCGCAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGttt > 1:2793038/1‑69 (MQ=255) cAATATGCGTATTCAGGATCTGGCGCAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGttt > 1:207220/1‑69 (MQ=255) cAATATGCGTATTCAGGATCTGGCGCAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGttt > 1:1840559/1‑69 (MQ=255) cAATATGCGTATTCAGGATCTGGCGCAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGttt > 1:1840267/1‑69 (MQ=255) cAATATGCGTATTCAGGATCTGGCGCAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGttt > 1:11311/1‑69 (MQ=255) cAATATGCGTATTCAGGATCTGGCGCAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGttt > 1:1203807/1‑69 (MQ=255) cAATATGCGTATTCAGGATCTGGCGCAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGttt > 1:1786230/1‑69 (MQ=255) cAATATGCGTATTCAGGATCTGGCGCAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGttt > 1:1642012/1‑69 (MQ=255) cAATATGCGTATTCAGGATCTGGCGCAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGttt > 1:939919/1‑69 (MQ=255) cAATATGCGTATTCAGGATCTGGCGCAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGttt > 1:1580771/1‑69 (MQ=255) cAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGttt < 1:940172/69‑1 (MQ=255) atatGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGa > 1:3322236/1‑69 (MQ=255) tatGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGAt < 1:3391591/69‑1 (MQ=255) tatGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGAt < 1:2919192/69‑1 (MQ=255) tatGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGAt < 1:74208/69‑1 (MQ=255) tatGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGAt < 1:596605/69‑1 (MQ=255) tatGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGAt < 1:2182004/69‑1 (MQ=255) tatGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGCTTTGAt < 1:438313/69‑1 (MQ=255) tatGCGTATTCAGGATCTATCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGAt < 1:817400/69‑1 (MQ=255) tGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTg > 1:2687959/1‑38 (MQ=255) tGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTaaa > 1:704387/1‑47 (MQ=255) tGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTc > 1:3364905/1‑57 (MQ=255) tGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATg > 1:1093129/1‑68 (MQ=255) tGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATg > 1:2101893/1‑68 (MQ=255) tGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGt > 1:2999728/1‑69 (MQ=255) tGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGt > 1:682406/1‑69 (MQ=255) tGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGt > 1:548204/1‑69 (MQ=255) tGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGt > 1:3245885/1‑69 (MQ=255) tGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGt > 1:2895109/1‑69 (MQ=255) tGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGt > 1:2540216/1‑69 (MQ=255) tGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGt > 1:1999496/1‑69 (MQ=255) tGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGt > 1:1031380/1‑69 (MQ=255) tGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGt > 1:1303644/1‑69 (MQ=255) tGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGt > 1:1410277/1‑69 (MQ=255) tGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGt > 1:1482493/1‑69 (MQ=255) tGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGt > 1:2115210/1‑69 (MQ=255) tGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGt > 1:2130087/1‑69 (MQ=255) tGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGt > 1:2721333/1‑69 (MQ=255) tGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGt > 1:2410641/1‑69 (MQ=255) cGTATTCAGGATCTGGCGCAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTg < 1:1594606/69‑1 (MQ=255) cGTATTCAGGATCTGGCGCAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTg < 1:2252667/69‑1 (MQ=255) cGTATTCAGGATCTGGCGCAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTg < 1:144571/69‑1 (MQ=255) cGTATTCAGGATCTGGCGCAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTg < 1:1297054/69‑1 (MQ=255) cGTATTCAGGATCTGGCGCAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTg < 1:2463030/69‑1 (MQ=255) gTATTCAGGATCTGGCGCAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCTGTTTGATGTTg < 1:2734357/68‑1 (MQ=255) gTATTCAGGATCTGGCGCAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTg < 1:1388090/68‑1 (MQ=255) tATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCg > 1:1493102/1‑69 (MQ=255) gATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTc > 1:2072335/1‑69 (MQ=255) gATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTc > 1:814995/1‑69 (MQ=255) gATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTc > 1:221428/1‑69 (MQ=255) gATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTc > 1:2230185/1‑69 (MQ=255) gATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTc > 1:1463675/1‑69 (MQ=255) tCTGGCGCAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCgg < 1:2900252/48‑1 (MQ=255) tCTGGCGCAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCgg < 1:918428/48‑1 (MQ=255) tCTGGCGCAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCgg < 1:3203002/48‑1 (MQ=255) tCTGGCGCAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCgg < 1:1018527/48‑1 (MQ=255) tCTGGCGCAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCgg < 1:1554158/48‑1 (MQ=255) tCTGGCGCAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCgg < 1:507541/48‑1 (MQ=255) tCTGGCGCAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCgg < 1:1712420/48‑1 (MQ=255) tCTGGCGCAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCgg < 1:1938270/48‑1 (MQ=255) tCTGGCGCAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCgg < 1:2893850/48‑1 (MQ=255) tCTGGCGCAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCgg < 1:3402950/48‑1 (MQ=255) tCTGGCGCAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCgg < 1:3369499/48‑1 (MQ=255) tCTGGCGCAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCgg < 1:3339878/48‑1 (MQ=255) tCTGGCGCAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCgg < 1:3294002/48‑1 (MQ=255) tCTGGCGCAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCgg < 1:305759/48‑1 (MQ=255) tGGCGCAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCgg < 1:476076/46‑1 (MQ=255) ggCACAAGGGATCCATAAGCTGATTCGTAAACAAGATCTTCCCGGTTTGAt < 1:1628340/51‑1 (MQ=255) | ATGCTACCCGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTC > minE/190208‑190333 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |