| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,269,258 | G→A | 21.9% | N16N (AAC→AAT) | birA ← | bifunctional biotin‑[acetylCoA carboxylase] holoenzyme synthetase and DNA‑binding transcriptional repressor, bio‑5'‑AMP‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,269,258 | 0 | G | A | 21.9% | 107.8 / 20.6 | 77 | N16N (AAC→AAT) | birA | bifunctional biotin‑[acetylCoA carboxylase] holoenzyme synthetase and DNA‑binding transcriptional repressor, bio‑5'‑AMP‑binding |
| Reads supporting (aligned to +/- strand): ref base G (31/29); new base A (5/12); total (36/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.68e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGTTTATTAATAGCCGCCCGGCTCATTCCCAGCGTTTCACCCAACTGCTCGCCAGAGTGAAATTCACCGTTCGCTAACAGGGCAATCAATTTCAGTGGCACGGTGTTATCCTTCATGAAATTGTCTCCAC > minE/2269190‑2269319 | tgtTTATTAATAGCCGCCCGGCTCATTCCCAGCGTTTCACCCAACTGCTCGCCAGAGTGAAATTCACCg > 1:826258/1‑69 (MQ=255) gccgccCGGCTCATTCCCAGCGTTTCACCCAACTGCTCGCCAGAGTGAAATTCACCGTTCGCt > 1:2343390/1‑63 (MQ=255) gccgccCGGCTCATTCCCAGCGTTTCACCCAACTGCTCGCCAGAGTGAAATTCACCGTTCGCt > 1:2060018/1‑63 (MQ=255) gccgccCGGCTCATTCCCAGCGTTTCACCCAACTGCTCGCCAGAGTGAAATTCACCGTTCGCt > 1:413533/1‑63 (MQ=255) gccgccCGGCTCATTCCCAGCGTTTCACCCAACTGCTCGCCAGAGTGAAATTCACCGTTCGCt > 1:721461/1‑63 (MQ=255) gccgccCGGCTCATTCCCAGCGTTTCACCCAACTGCTCGCCAGAGTGAAATTCACCGTCCGCt > 1:2962367/1‑63 (MQ=255) cTCATTCCCAGCGTTTCACCCAACTGCTCGCCAGAGTGAAATTCACCGTTCGCt < 1:2185855/54‑1 (MQ=255) cTCATTCCCAGCGTTTCACCCAACTGCTCGCCAGAGTGAAATTCACCGTTCGCt < 1:1084405/54‑1 (MQ=255) cTCATTCCCAGCGTTTCACCCAACTGCTCGCCAGAGTGAAATTCACCGTTCGCt < 1:251263/54‑1 (MQ=255) cTCATTCCCAGCGTTTCACCCAACTGCTCGCCAGAGTGAAATTCACCGTTCGCt < 1:200498/54‑1 (MQ=255) tCCCAGCGTTTCACCCAACTGCTCGCCAGAGTGAAATTCACCGTTCGCTAACAGGGCAATCAATTTCAg < 1:653288/69‑1 (MQ=255) cccAGCGTTTCACCCAACTGCTCGCCAGAGTGAAATTCACCGTTCGCTAACAGGGCAATCAATTTCAGt > 1:952544/1‑69 (MQ=255) cccAGCGTTTCACCCAACTGCTCGCCAGAGTGAAATTCACCGTTCGCTAACAGGGCAATCAATTTCAGt > 1:2772770/1‑69 (MQ=255) cccAGCGTTTCACCCAACTGCTCGCCAGAGTGAAATTCACCGTTCGCTAACAGGGCAATCAATTTCAGt > 1:1777662/1‑69 (MQ=255) cccAGCGTTTCACCCAACTGCTCGCCAGAGTGAAATTCACCGTTCGCTAACAGGGCAATCAATTTCAGt > 1:728670/1‑69 (MQ=255) cccAGCGTTTCACCCAACTGCTCGCCAGAGTGAAATTCACCGTTCGCTAACAGGGCAATCAATTTCAGt > 1:884603/1‑69 (MQ=255) aGCGTTTCACCCAACTGCTCGCCAGAGTGAAATTCACCGTTCGCTAACAGGGCAATCAATTTCAGTgg < 1:42059/68‑1 (MQ=255) aGCGTTTCACCCAACTGCTCGCCAGAGTGAAATTCACCGTTCGCTAACAGGGCAATCAATTTCAGTgg < 1:2287948/68‑1 (MQ=255) aGCGTTTCACCCAACTGCTCGCCAGAGTGAAATTCACCGTTCGCTAACAGGGCAATCAATTTCAGTgg < 1:2818487/68‑1 (MQ=255) aGCGTTTCACCCAACTGCTCGCCAGAGTGAAATTCACCGTTCGCTAACAGGGCAATCAATTTCAGTgg < 1:2050086/68‑1 (MQ=255) aGCGTTTCACCCAACTGCTCGCCAGAGTGAAATTCACCGTTCGCTAACAGGGCAATCAATTTCAGTgg < 1:3392865/68‑1 (MQ=255) tCACCCAACTGCTCGCCAGAGTGAAATTCACCGTTCGCTAACAGGGcaatc > 1:759568/1‑51 (MQ=255) tCACCCAACTGCTCGCCAGAGTGAAATTCACCGTTCGCTAACAGGGcaatc > 1:1409837/1‑51 (MQ=255) tCACCCAACTGCTCGCCAGAGTGAAATTCACCGTTCGCTAACAGGGcaatc > 1:2879322/1‑51 (MQ=255) tCACCCAACTGCTCGCCAGAGTGAAATTCACCGTTCGCTAACAGGGcaatc > 1:846617/1‑51 (MQ=255) tCACCCAACTGCTCGCCAGAGTGAAATTCACCGTTCGCTAACAGGGcaatc > 1:184015/1‑51 (MQ=255) tCACCCAACTGCTCGCCAGAGTGAAATTCACCGTTCGCTAACAGGGcaatc > 1:1575645/1‑51 (MQ=255) tCACCCAACTGCTCGCCAGAGTGAAATTCACCGTTCGCTAACAGGGcaatc > 1:892279/1‑51 (MQ=255) tCACCCAACTGCTCGCCAGAGTGAAATTCACCGTTCGCTAACAGGGcaatc > 1:167284/1‑51 (MQ=255) aCCCAACTGCTCGCCAGAGTGAAATTCACCGTTCGCTAACAGGGCAATCAATTTCAGTgg < 1:1087752/60‑1 (MQ=255) cGCCAGAGTGAAATTCGCCATTCGCTAACAGGGCGATCAATTTCAGTGGCACGGTGTTATCCTTCAt < 1:327714/67‑1 (MQ=255) cGCCAGAGTGAAATTCGCCATTCGCTAACAGGGCGATCAATTTCAGTGGCACGGTGTTATCCTTCAt < 1:968333/67‑1 (MQ=255) cGCCAGAGTGAAATTCGCCATTCGCTAACAGGGCGATCAATTTCAGTGGCACGGTGTTATCCTTCAt < 1:2271826/67‑1 (MQ=255) gagTGAAATTCACCGTTCGCTAACAGGGCAATCAATTTCAGTGGCACGGTGTTATCCTTCATGAAATTg < 1:1416126/69‑1 (MQ=255) gagTGAAATTCACCGTTCGCTAACAGGGCAATCAATTTCAGTGGCACGGTGTTATCCTTCATGAAATTg < 1:3281668/69‑1 (MQ=255) gagTGAAATTCACCGTTCGCTAACAGGGCAATCAATTTCAGTGGCACGGTGTTATCCTTCATGAAATTg < 1:3212610/69‑1 (MQ=255) gagTGAAATTCACCGTTCGCTAACAGGGCAATCAATTTCAGTGGCACGGTGTTATCCTTCATGAAATTg < 1:2587799/69‑1 (MQ=255) gTGAAATTCGCCATTCGCTAACAGGGCGATCAATTTCAGTGGCACGGTGTTATCCTTCATGAAATTGtc < 1:1016201/69‑1 (MQ=255) gTGAAATTCGCCATTCGCTAACAGGGCGATCAATTTCAGTGGCACGGTGTTATCCTTCATGAAATTGtc < 1:896209/69‑1 (MQ=255) gTGAAATTCGCCATTCGCTAACAGGGCGATCAATTTCAGTGGCACGGTGTTATCCTTCATGAAATTGtc < 1:500443/69‑1 (MQ=255) gTGAAATTCGCCATTCGCTAACAGGGCGATCAATTTCAGTGGCACGGTGTTATCCTTCATGAAATTGtc < 1:3083581/69‑1 (MQ=255) gTGAAATTCGCCATTCGCTAACAGGGCGATCAATTTCAGTGGCACGGTGTTATCCTTCATGAAATTGtc < 1:2734258/69‑1 (MQ=255) gTGAAATTCGCCATTCGCTAACAGGGCGATCAATTTCAGTGGCACGGTGTTATCCTTCATGAAATTGtc < 1:2577900/69‑1 (MQ=255) gTGAAATTCGCCATTCGCTAACAGGGCGATCAATTTCAGTGGCACGGTGTTATCCTTCATGAAATTGtc < 1:2327272/69‑1 (MQ=255) gTGAAATTCGCCATTCGCTAACAGGGCGATCAATTTCAGTGGCACGGTGTTATCCTTCATGAAATTGtc < 1:2331205/69‑1 (MQ=255) gTGAAATTCACCGTTCGCTAACAGGGCAATCAATTTCAGTGGCACGGTGTTATCCTTCATGAAATTGtg > 1:3031970/1‑68 (MQ=255) gTGAAATTCACCGTTCGCTAACAGGGCAATCAATTTCAGTGGCACGGTGTTATCCTTCATGAAATTGtc > 1:2788469/1‑69 (MQ=255) gTGAAATTCACCGTTCGCTAACAGGGCAATCAATTTCAGTGGCACGGTGTTATCCTTCATGAAATTGtc < 1:2019777/69‑1 (MQ=255) gTGAAATTCACCGTTCGCTAACAGGGCAATCAATTTCAGTGGCACGGTGTTATCCTTCATGAAATTGtc > 1:421142/1‑69 (MQ=255) gTGAAATTCACCGTTCGCTAACAGGGCAATCAATTTCAGTGGCACGGTGTTATCCTTCATGAAATTGtc > 1:189070/1‑69 (MQ=255) tGAAATTCGCCATTCGCTAACAGGGCGATCAATTTCAGTGGCACGGTGTTATACTTCATGAAATTGtc < 1:2097950/68‑1 (MQ=255) tGAAATTCACCGTTCGCTAACAGGGCAATCAATTTCAGTGGCACGGTGTTATCCTTCATGAAATTGtc > 1:257727/1‑68 (MQ=255) tGAAATTCACCGTTCGCTAACAGGGCAATCAATTTCAGTGGCACGGTGTTATCCTTCATGAAATTGtc > 1:2280857/1‑68 (MQ=255) tGAAATTCACCGTTCGCTAACAGGGCAATCAATTTCAGTGGCACGGTGTTATCCTTCATGAAATTGtc > 1:1070823/1‑68 (MQ=255) tGAAATTCACCGTTCGCTAACAGGGCAATCAATTTCAGTGGCACGGTGTTATCCTTCATGAAATTGtc > 1:1239524/1‑68 (MQ=255) tGAAATTCACCGTTCGCTAACAGGGCAATCAATTTCAGTGGCACGGTGTTATCCTTCATGAAATTGtc > 1:2133218/1‑68 (MQ=255) tGAAATTCACCGTTCGCTAACAGGGCAATCAATTTCAGTGGCACGGTGTTATCCTTCATGAAATTGtc > 1:2860592/1‑68 (MQ=255) tGAAATTCACCGTTCGCTAACAGGGCAATCAATTTCAGTGGCACGGTGTTATCCTTCATGAAATTGtc > 1:1986830/1‑68 (MQ=255) aaaTTCACCGTTCGCTAACAGGGCAATCAATTTCAGTGGCACGGTGTTa > 1:1029879/1‑49 (MQ=255) aaaTTCACCGTTCGCTAACAGGGCAATCAATTTCAGTGGCACGGTGTTa > 1:796557/1‑49 (MQ=255) aTTCACCGTTCGCTAACAGGGCAATCAATTTCAGTGGCACGGTGTTATCCTTCATGAAATTGTCTCCAc < 1:168927/69‑1 (MQ=255) aTTCACCGTTCGCTAACAGGGCAATCAATTTCAGTGGCACGGTGTTATCCTTCATGAAATTGTCTCCAc < 1:1104584/69‑1 (MQ=255) aTTCACCGTTCGCTAACAGGGCAATCAATTTCAGTGGCACGGTGTTATCCTTCATGAAATTGTCTCCAc < 1:1152416/69‑1 (MQ=255) aTTCACCGTTCGCTAACAGGGCAATCAATTTCAGTGGCACGGTGTTATCCTTCATGAAATTGTCTCCAc < 1:3112750/69‑1 (MQ=255) aTTCACCGTTCGCTAACAGGGCAATCAATTTCAGTGGCACGGTGTTATCCTTCATGAAATTGTCTCCAc < 1:1691266/69‑1 (MQ=255) aTTCACCGTTCGCTAACAGGGCAATCAATTTCAGTGGCACGGTGTTATCCTTCATGAAATTGTCTCCAc < 1:2206439/69‑1 (MQ=255) aTTCACCGTTCGCTAACAGGGCAATCAATTTCAGTGGCACGGTGTTATCCTTCATGAAATTGTCTCCAc < 1:3108909/69‑1 (MQ=255) aTTCACCGTTCGCTAACAGGGCAATCAATTTCAGTGGCACGGTGTTATCCTTCATGAAATTGTCTCCAc < 1:191345/69‑1 (MQ=255) aTTCACCGTTCGCTAACAGGGCAATCAATTTCAGTGGCACGGTGTTATCCTTCATGAAATTGTCTCCAc < 1:2163263/69‑1 (MQ=255) ttCGCCATTCGCTAACAGGGCGATCAATTTCAGTGGCACg > 1:2302347/1‑40 (MQ=37) ttCGCCATTCGCTAACAGGGCGATCAATTTCAGTGGCACg > 1:777402/1‑40 (MQ=37) ttCGCCATTCGCTAACAGGGCGATCAATTTCAGTGGCACGGTGTTATCCTTCATGaagga > 1:2295394/1‑57 (MQ=255) ttCGCCATTCGCTAACAGGGCGATCAATTTCAGTGGCACGGTGTTATCCTTCATGaagga > 1:3045782/1‑57 (MQ=255) ttCGCCATTCGCTAACAGCGCGATCAATTTCAGAGGCACGGTGTTATCCt > 1:1611131/1‑50 (MQ=25) ttCACCGTTCGCTAACAGGGCAATCAATTTCAGTGGCACGGTGTTATCCTTCATGAAATTGTCTCCAc < 1:164792/68‑1 (MQ=255) ttCACCGTTCGCTAACAGGGCAATCAATTTCAGTGGCACGGTGTTATCCTTCATGAAATTGTCTCCAc < 1:464960/68‑1 (MQ=255) ttCACCGTTCGCTAACAGGGCAATCAATTTCAGTGGCACGGTGTTATCCTTCATGAAATTGTCTCCAc < 1:1773706/68‑1 (MQ=255) ttCACCGTTCGCTAACAGGGCAATCAATTTCAGTGGCACGGTGTTATCCTTCATGAAATTGTCTCCAc < 1:234338/68‑1 (MQ=255) | TGTTTATTAATAGCCGCCCGGCTCATTCCCAGCGTTTCACCCAACTGCTCGCCAGAGTGAAATTCACCGTTCGCTAACAGGGCAATCAATTTCAGTGGCACGGTGTTATCCTTCATGAAATTGTCTCCAC > minE/2269190‑2269319 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |