| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,276,970 | A→G | 21.1% | Y593Y (TAT→TAC) | btuB ← | vitamin B12/cobalamin outer membrane transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,276,970 | 0 | A | G | 21.1% | 234.7 / 49.0 | 165 | Y593Y (TAT→TAC) | btuB | vitamin B12/cobalamin outer membrane transporter |
| Reads supporting (aligned to +/- strand): ref base A (54/76); new base G (19/16); total (73/92) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.86e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 165 total aligned reads displayed.
GCCAGACAAGGTGTATTCCCGTCCTGCAGTTTGGTAGCCATAGACTGTCTCATAATCTTTGTCGAACAGGTTGGCTATTTTACCACGAACTGTCAGGTGAGAGGTGACCGGATA > minE/2276919‑2277032 | gCCAGACAAGGTGTATTCCCGTCCTGCAGTTTGGTAGCCATAGACTGTCTCATAATCttt > 1:100820/1‑60 (MQ=255)gCCAGACAAGGTGTATTCCCGTCCTGCAGTTTGGTAGCCATAGACTGTCTCATAATCTTTGTCGAACAg > 1:1083394/1‑69 (MQ=255)gCCAGACAAGGTGTATTCCCGTCCTGCAGTTTGGTAGCCATAGACTGTCTCATAATCTTTGTCGAACAg > 1:2603710/1‑69 (MQ=255)gCCAGACAAGGTGTATTCCCGTCCTGCAGTTTGGTAGCCATAGACTGTCTCATAATCTTTGTCGAACAg > 1:1274382/1‑69 (MQ=255)gCCAGACAAGGTGTATTCCCGTCCTGCAGTTTGGTAGCCATAGACTGTCTCATAATCTTTGTCGAACAg > 1:1480313/1‑69 (MQ=255)gCCAGACAAGGTGTATTCCCGTCCTGCAGTTTGGTAGCCATAGACTGTCTCATAATCTTTGTCGAACAg > 1:2304805/1‑69 (MQ=255) cAAGGTGTATTCCCGTCCTGCAGTTTGGTAGCCATAGACTGTCTCATAATCTTTGTCGAACAGGTTGGc < 1:1036162/69‑1 (MQ=255) cAAGGTGTATTCCCGTCCTGCAGTTTGGTAGCCATAGACTGTCTCATAATCTTTGTCGAACAGGTTGGc < 1:713391/69‑1 (MQ=255) cAAGGTGTATTCCCGTCCTGCAGTTTGGTAGCCATAGACTGTCTCATAATCTTTGTCGAACAGGTTGGc < 1:937805/69‑1 (MQ=255) cAAGGTGTATTCCCGTCCTGCAGTTTGGTAGCCATAGACTGTCTCATAATCTTTGTCGAACAGGTTGGc > 1:1652563/1‑69 (MQ=255) cAAGGTGTATTCCCGTCCTGCAGTTTGGTAGCCATAGACTGTCTCATAATCTTTGTCGAACACGTTGGc < 1:441992/69‑1 (MQ=255) aaGGTATATTCCCGTCCTGCAGTTTGGTAGCCATAGACTGTCTCGTAATCTTTGTCGAACAGGTTGGCt < 1:794261/69‑1 (MQ=255) aaGGTATATTCCCGTCCTGCAGTTTGGTAGCCATAGACTGTCTCGTAATCTTTGTCGAACAGGTTGGCt < 1:1753652/69‑1 (MQ=255) aaGGTATATTCCCGTCCTGCAGTTTGGTAGCCATAGACTGTCTCGTAATCTTTGTCGAACAGGTTGGCt < 1:1699328/69‑1 (MQ=255) aaGGTATATTCCCGTCCTGCAGTTTGGTAGCCATAGACTGTCTCGTAATCTTTGTCGAACAGGTTGGCt < 1:2734112/69‑1 (MQ=255) aaGGTATATTCCCGTCCTGCAGTTTGGTAGCCATAGACTGTCTCGTAATCTTTGTCGAACAGGTTCGCt < 1:1769107/69‑1 (MQ=255) aGGTGTATTCCCGTCCTGCAGTTTGGTAGCCATAGACTGTCTCATAATCTTTGTCGAACAGGTTGGc < 1:3378428/67‑1 (MQ=255) aGGTGTATTCCCGTCCTGCAGTTTGGTAGCCATAGACTGTCTCATAATCTTTGTCGAACAGGTTGGc < 1:290676/67‑1 (MQ=255) tgtATTCCCGTCCTGCAGTTTGGTAGCCATAGACTGTCTCATAATCTTTGTCGAACAGGTTGGCTAttt < 1:2238085/69‑1 (MQ=255) tgtATTCCCGTCCTGCAGTTTGGTAGCCATAGACTGTCTCATAATCTTTGTCGAACAGGTTGGCTAttt < 1:637616/69‑1 (MQ=255) tatATTCCCGTCCTGCAGTTTGGTAGCCATAGACTGTCTGGTAATCTTTGTCGAACAGGTTGGCTAttt > 1:164413/3‑69 (MQ=255) tatATTCCCGTCCTGCAGTTTGGTAGCCATAGACTGTCTCGTAATCTTTGTCGAACAGGTTGGCTAttt > 1:2692269/3‑69 (MQ=255) tatATTCCCGTCCTGCAGTTTGGTAGCCATAGACTGTCTCGTAATCTTTGTCGAACAGGTTGGCTAttt > 1:2288236/3‑69 (MQ=255) tatATTCCCGTCCTGCAGTTTGGTAGCCATAGACTGTCTCGTAATCTTTGTCGAACAGGTTGGCTAttt > 1:2241630/3‑69 (MQ=255) tatATTCCCGTCCTGCAGTTTGGTAGCCATAGACTGTCTCGTAATCTTTGTCGAACAGGTTGGCTAttt > 1:360256/3‑69 (MQ=255) tatATTCCCGTCCTGCAGTTTGGTAGCCATAGACTGTCTCGTAATCTTTGTCGAACAGGTTGGCTAttt > 1:3031140/3‑69 (MQ=255) tatATTCCCGTCCTGCAGTTTGGTAGCCATAGACTGTCTCGTAATCTTTGTCGAACAGGTTGGCTAttt > 1:3015060/3‑69 (MQ=255) tatATTCCCGTCCTGCAGTTTGGTAGCCATAGACTGTCTCGTAATCTTTGTCGAACAGGTTGGCTAttt > 1:2150824/3‑69 (MQ=255) tatATTCCCGTCCTGCAGTTTGGTAGCCATAGACTGTCTCGTAATCTTTGTCGAACAGGTTGGCTAttt > 1:2599597/3‑69 (MQ=255) tatATTCCCGTCCTGCAGTTTGGTAGCCATAGACTGTCTCGTAATCTTTGTCGAACAGGTTGGCTAttt > 1:693966/3‑69 (MQ=255) tatATTCCCGTCCTGCAGTTTGGTAGCCATAGACTGTCTCGTAATCTTTGTCGAACAGGTTGGCTAttt > 1:1231730/3‑69 (MQ=255) tatATTCCCGTCCTGCAGTTTGGTAGCCATAGACTGTCTCGTAATCTTTGTCGAACAGGTTGGCTAttt > 1:1725366/3‑69 (MQ=255) ttCCCGTCCTGCAGTTTGGTAGCCATAGACTGTCTCATAATCTTTGTCGAACAGGTTGGCTATTTTAcc < 1:878659/69‑1 (MQ=255) ttCCCGTCCTGCAGTTTGGTAGCCATAGACTGTCTCATAATCTTTGTCGAACAGGTTGGCTATTTTAcc < 1:2412319/69‑1 (MQ=255) ttCCCGTCCTGCAGTTTGGTAGCCATAGACTGTCTCATAATCTTTGTCGAACAGGTTGGCTATTTTAcc < 1:1582887/69‑1 (MQ=255) ttCCCGTCCTGCAGTTTGGTAGCCATAGACTGTCTCATAATCTTTGTCGAACAGGTTGGCTATTTTAcc < 1:2216880/69‑1 (MQ=255) ttCCCGTCCTGCAGTTTGGTAGCCATAGACTGTCTCATAATCTTTGTCGAACAGGTTGGCTATTTTAcc < 1:536026/69‑1 (MQ=255) ttCCCGTCCTCCAGTTTGGTAGCCATAGACTGTCTCATAATCTTTGTCGAACAGGTTGGCTATTTTAcc < 1:3398184/69‑1 (MQ=255) cccGTCCTGCAGTTTGGTAGCCATAGACTGTCTCATAATCTTTGTCGAACAGGTTGGCTATTTTACCa > 1:2188667/1‑68 (MQ=255) cccGTCCTGCAGTTTGGTAGCCATAGACTGTCTCATAATCTTTGTCGAACAGGTTGGCTATTTTACCa > 1:1176366/1‑68 (MQ=255) cccGTCCTGCAGTTTGGTAGCCATAGACTGTCTCATAATCTTTGTCGAACAGGTTGGCTATTTTACCa > 1:92848/1‑68 (MQ=255) cccGTCCTGCAGTTTGGTAGCCATAGACTGTCTCATAATCTTTGTCGAACAGGTTGGCTATTTTACCa > 1:3182352/1‑68 (MQ=255) cccGTCCTGCAGTTTGGTAGCCATAGACTGTCTCATAATCTTTGTCGAACAGGTTGGCTATTTTACCa > 1:497454/1‑68 (MQ=255) cccGTCCTGCAGTTTGGTAGCCATAGACTGTCTCATAATCTTTGTCGAACAGGTTGGCTATTTTACCa > 1:2341145/1‑68 (MQ=255) cccGTCCTGCAGTTTGGTAGCCATAGACTGTCTCATAATCTTTGTCGAACAGGTTGGCTATTTTACCa > 1:1451209/1‑68 (MQ=255) cccGTCCTGCAGTTTGGTAGCCATAGACTGTCTCATAATCTTTGTCGAACAGGTTGGCTATTTTACCa > 1:2034433/1‑68 (MQ=255) cccGTCCTGCAGTTTGGTAGCCATAGACTGTCTCATAATCTTTGTCGAACAGGTTGGCTATTTTACCa > 1:1706081/1‑68 (MQ=255) cGTCCTGCAGTTTGGTAGCCATAGACTGTCTCATAATCTTTGTCGAACAGGTTGGCTATTTTACCACg < 1:1853324/68‑1 (MQ=255) ccTGCAGTTTGGTAGCCATTGACTGTCTCATAATCTTTGTCGAACAGGTTGGCTATTTTACCTCGAACt < 1:7455/69‑1 (MQ=255) ccTGCAGTTTGGTAGCCATAGACTGTCTCATAATCTTTGTCGAACAGGTTGGCTATTTTACCACGAACt < 1:2401947/69‑1 (MQ=255) ccTGCAGTTTGGTAGCCATAGACTGTCTCATAATCTTTGTCGAACAGGTTGGCTATTTTACCACGAACt < 1:748627/69‑1 (MQ=255) ccTGCAGTTTGGTAGCCATAGACTGTCTCATAATCTTTGTCGAACAGGTTGGCTATTTTACCACGAACt < 1:1293952/69‑1 (MQ=255) ccTGCAGTTTGGTAGCCATAGACTGTCTCATAATCTTTGTCGAACAGGTTGGCTATTTTACCACGAACt < 1:374610/69‑1 (MQ=255) ccTGCAGTTTGGTAGCCATAGACTGTCTCATAATCTTTGTCGAACAGGTTGGCTATTTTACCACGAACt < 1:1170755/69‑1 (MQ=255) ccTGCAGTTTGGTAGCCATAGACTGTCTCATAATCTTTGTCGAACAGGTTGGCTATTTTACCACGAACt < 1:1067743/69‑1 (MQ=255) gCAGTTTGGTAGCCATAGACTGTCTCATAATCTTTGTCGAACAGGTTGGCTATTTTACCACGAACTGTc < 1:917479/69‑1 (MQ=255) gCAGTTTGGTAGCCATAGACTGTCTCATAATCTTTGTCGAACAGGTTGGCTATTTTACCACGAACTGTc < 1:985830/69‑1 (MQ=255) gCAGTTTGGTAGCCATAGACTGTCTCATAATCTTTGTCGAACAGGTTGGCTATTTTACCACGAACTGTc < 1:1834395/69‑1 (MQ=255) gTTTGGTAGCCATAGACTGTCTCATAATCTTTGTCGAACAGGTTGGCTATTTTACCACGAACTGTCAg < 1:2877025/68‑1 (MQ=255) gTTTGGTAGCCATAGACTGTCTCATAATCTTTGTCGAACAGGTTGGCTATTTTACCACGAACTGTCAg < 1:3411047/68‑1 (MQ=255) gTTTGGTAGCCATAGACTGTCTCATAATCTTTGTCGAACAGGTTGGCTATTTTACCACGAACTGTCAg < 1:310865/68‑1 (MQ=255) gTTTGGTAGCCATAGACTGTCTCATAATCTTTGTCGAACAGGTTGGCTATTTTACCACGAACTGTCAg < 1:2533109/68‑1 (MQ=255) gTTTGGTAGCCATAGACTGTCTCATAATCTTTGTCGAACAGGTTGGCTATTTTACCACGAACTGTCAg < 1:2983163/68‑1 (MQ=255) ttGGTAGGCTTAGACTGTCTCATAATCTTTGTCGAACAGGTTGGCTATTTTACCAAGAACTGTCAg < 1:1251310/66‑1 (MQ=255) ttGGTAGCCATAGACTGTCTCATAATCTTTGTCGAACAGGTTGGCTAtttt < 1:2622557/51‑1 (MQ=255) ttGGTAGCCATAGACTGTCTCATAATCTTTGTCGAACAGGTTGGCTAtttt < 1:143573/51‑1 (MQ=255) ttGGTAGCCATAGACTGTCTCATAATCTTTGTCGAACAGGTTGGCTAtttt < 1:426828/51‑1 (MQ=255) ttGGTAGCCATAGACTGTCTCATAATCTTTGTCGAACAGGTTGGCTAtttt < 1:2063280/51‑1 (MQ=255) ttGGTAGCCATAGACTGTCTCATAATCTTTGTCGAACAGGTTGGCTAtttt < 1:2673604/51‑1 (MQ=255) tGGTAGCCATAGACTGTCTCATAATCTTTGTCGAACAGGTTGGCTATTTTACCACGAACTGTCAGGt > 1:843269/1‑67 (MQ=255) tGGTAGCCATAGACTGTCTCATAATCTTTGTCGAACAGGTTGGCTATTTTACCACGAACTGTCAGGt > 1:1362692/1‑67 (MQ=255) tGGTAGCCATAGACTGTCTCATAATCTTTGTCGAACAGGTTGGCTATTTTACCACGAACTGTCAGGt > 1:2897766/1‑67 (MQ=255) tGGTAGCCATAGACTGTCTCATAATCTTTGTCGAACAGGTTGGCTATTTTACCACGAACTGTCAGGt > 1:2846336/1‑67 (MQ=255) gCCATAGACTGTCTCGTAATCTTTGTCGAACAGGTTGGCt < 1:1057268/40‑1 (MQ=255) cATAGACTGTCTCATAATCTTTGTCGAACAGGTTGGc < 1:1204813/37‑1 (MQ=255) cATAGACTGTCTCATAATCTTTGTCGAACAGGTTGGc < 1:1334867/37‑1 (MQ=255) cATAGACTGTCTCATAATCTTTGTCGAACAGGTTGGc < 1:2489708/37‑1 (MQ=255) cATAGACTGTCTCATAATCTTTGTCGAACAGGTTGGc < 1:1536362/37‑1 (MQ=255) cATAGACTGTCTCATAATCTTTGTCGAACAGGTTGGc < 1:2878931/37‑1 (MQ=255) cATAGACTGTCTCATAATCTTTGTCGAACAGGTTGGc < 1:587781/37‑1 (MQ=255) cATAGACTGTCTCATAATCTTTGTCGAACAGGTTGGc < 1:1904589/37‑1 (MQ=255) tAGACTGTCTCATAATCTTTGTCGAACAGGTTGGc < 1:2910373/35‑1 (MQ=255) gACTGTCTCATAATCTTTGTCGAACAGGTTGGCTATTTTACCACGAACTGTCAGGTGAGAGGTGACCg > 1:1888004/1‑68 (MQ=255) gACTGTCTCATAATCTTTGTCGAACAGGTTGGCTATTTTACCACGAACTGTCAGGTGAGAGGTGACCg > 1:2575952/1‑68 (MQ=255) gACTGTCTCATAATCTTTGTCGAACAGGTTGGCTATTTTACCACGAACTGTCAGGTGAGAGGTGACCg > 1:2621861/1‑68 (MQ=255) gACTGTCTCATAATCTTTGTCGAACAGGTTGGCTATTTTACCACGAACTGTCAGGTGAGAGGTGACCg > 1:2514563/1‑68 (MQ=255) gACTGTCTCATAATCTTTGTCGAACAGGTTGGCTATTTTACCACGAACTGTCAGGTGAGAGGTGACCg > 1:325679/1‑68 (MQ=255) gACTGTCTCATAATCTTTGTCGAACAGGTTGGCTATTTTACCACGAACTGTCAGGTGAGAGGTGACCg > 1:2510680/1‑68 (MQ=255) gACTGTCTCATAATCTTTGTCGAACAGGTTGGCTATTTTACCACGAACTGTCAGGTGAGAGGTGACCg > 1:1841964/1‑68 (MQ=255) gACTGTCTCATAATCTTTGTCGAACAGGTTGGCTATTTTACCACGAACTGTCAGGTGAGAGGTGACCg > 1:1879557/1‑68 (MQ=255) gACTGTCTCATAATCTTTGTCGAACAGGTTGGCTATTTTACCACGAACTGTCAGGTGAGAGGTGACCg > 1:2049211/1‑68 (MQ=255) gACTGTCTCATAATCTTTGTCGAACAGGTTGGCTATTTTACCACGAACTGTCAGGTGAGAGGTGACCg > 1:584057/1‑68 (MQ=255) gACTGTCTCATAATCTTTGTCGAACAGGTTGGCTATTTTACCACGAACTGTCAGGTGAGAGGTGACCg > 1:549435/1‑68 (MQ=255) gACTGTCTCATAATCTTTGTAGAACAGGTTGGCTATTTTACCACGAACTGTCAGGTGAGAGGTGACCg > 1:222805/1‑68 (MQ=255) cTGTCTCATAATCTTTGTCGAACAGGTTGGCTATTTTACCACGAACTGTCAGGTGAGAGGTGACCGGa < 1:1224586/68‑1 (MQ=255) tGTCTCATAATCTTTGTCGAACAGGTTGGCTATTTTACCACGAACTGTCAGGTGAGAGGTGACCGGa < 1:2847822/67‑1 (MQ=255) tGTCTCATAATCTTTGTCGAACAGGTTGGCTATTTTACCACGAACTGTCAGGTGAGAGGTGACCGGATa > 1:663214/1‑69 (MQ=255) tGTCTCATAATCTTTGTCGAACAGGTTGGCTATTTTACCACGAACTGTCAGGTGAGAGGTGACCGGATa > 1:2777700/1‑69 (MQ=255) tGTCTCATAATCTTTGTCGAACAGGTTGGCTATTTTACCACGAACTGTCAGGTGAGAGGTGACCGGATa > 1:317308/1‑69 (MQ=255) tGTCTCATAATCTTTGTCGAACAGGTTGGCTATTTTACCACGAACTGTCAGGTGAGAGGTGACCGGATa > 1:52056/1‑69 (MQ=255) | GCCAGACAAGGTGTATTCCCGTCCTGCAGTTTGGTAGCCATAGACTGTCTCATAATCTTTGTCGAACAGGTTGGCTATTTTACCACGAACTGTCAGGTGAGAGGTGACCGGATA > minE/2276919‑2277032 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |