| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,277,216 | G→A | 26.8% | T511T (ACC→ACT) | btuB ← | vitamin B12/cobalamin outer membrane transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,277,216 | 0 | G | A | 26.8% | 147.9 / 49.4 | 126 | T511T (ACC→ACT) | btuB | vitamin B12/cobalamin outer membrane transporter |
| Reads supporting (aligned to +/- strand): ref base G (42/50); new base A (27/7); total (69/57) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.07e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.11e-01 | |||||||||||
Only 100 of 126 total aligned reads displayed.
ATACAACTGCCAGTCGAGCTGGTATTTCACCTGCTGTTTAGCACGGCGTAACAACGGCGTGTCGGTAATCGCATTGCGCGCATCGACATAATCATAACTCACAGTATGCGTCAGTGGTCCGG > minE/2277153‑2277274 | aTACAACTGCCAGTCGAGCTGGTATTTCACCTGCTGTTTAGCACGGCGTAACAACGGCGTGTCGGTAAt > 1:910881/1‑69 (MQ=255)aTACAACTGCCAGTCGAGCTGGTATTTCACCTGCTGTTTAGCACGGCGTAACAACGGCGTGTCGGTAAt > 1:2014359/1‑69 (MQ=255)aTACAACTGCCAGTCGAGCTGGTATTTCACCTGCTGTTTAGCACGGCGTAACAACGGCGTGTCGGTAAt > 1:252356/1‑69 (MQ=255)aTACAACTGCCAGTCGAGCTGGTATTTCACCTGCTGTTTAGCACGGCGTAACAACGGCGTGTCGGTAAt > 1:1534245/1‑69 (MQ=255)aTACAACTGCCAGTCGAGCTGGTATTTCACCTGCTGTTTAGCACGGCGTAACAACGGCGTGTCGGTAAt > 1:1533379/1‑69 (MQ=255)aTACAACTGCCAGTCGAGCTGGTATTTCACCTGCTGTTTAGCACGGCGTAACAACGGCGTGTCGGTAAt > 1:1518527/1‑69 (MQ=255)aTACAACTGCCAGTCGAGCTGGTATTTCACCTGCTGTTTAGCACGGCGTAACAACGGCGTGTCGGTAAt > 1:3407860/1‑69 (MQ=255)aTACAACTGCCAGTCGAGCTGGTATTTCACCTGCTGTTTAGCACGGCGTAACAACGGCGTGTCGGTAAt > 1:894200/1‑69 (MQ=255) aCAACTGCCAGTCGAGCTGGTATTTCACCTGCTGTTTAGCACGGCGTAACAACGGCGTGTCGGTAATCg < 1:3405583/69‑1 (MQ=255) aCAACTGCCAGTCGAGCTGGTATTTCACCTGCTGTTTAGCACGGCGTAACAACGGCGTGTCGGTAATCg < 1:188205/69‑1 (MQ=255) aCAACTGCCAGTCGAGCTGGTATTTCACCTGCTGTTTAGCACGGCGTAACAACGGCGTGTCGGTAATCg < 1:2751965/69‑1 (MQ=255) aCAACTGCCAGTCGAGCTGGTATTTCACCTGCTGTTTAGCACGGCGTAACAACGGCGTGTCGGTAATCg < 1:2562562/69‑1 (MQ=255) aCAACTGCCAGTCGAGCTGGTATTTCACCTGCTGTTTAGCACGGCGTAACAACGGCGTGTCGGTAATCg < 1:2125548/69‑1 (MQ=255) cAACTGCCAGTCGAGCTGGTATTTCACCTGCTGTTTAGCACGGCGTAACAACGGCGTGTCGGTAATCGc > 1:1820381/1‑69 (MQ=255) cAACTGCCAGTCGAGCTGGTATTTCACCTGCTGTTTAGCACGGCGTAACAACGGCGTGTCGGTAATCGc > 1:2870250/1‑69 (MQ=255) tGCCAGTCGTGCTGGTATTTCACCTGCTGTTTAGCACGGCGTAACAACGGCGTGTCGGTAATCGCATTg < 1:499796/69‑1 (MQ=255) tGCCAGTCGAGCTGGTATTTGACCTGCTGTTTAGCACGGCGTAACAACGGCGTGTCGGTAATCGCATTg < 1:3056550/69‑1 (MQ=255) tGCCAGTCGAGCTGGTATTTCACCTGCTGTTTAGCACGGCGTAACAACGGCGTGTCGGTAATCGCATTg < 1:697624/69‑1 (MQ=255) tGCCAGTCGAGCTGGTATTTCACCTGCTGTTTAGCACGGCGTAACAACGGCGTGTCGGTAATCGCATTg < 1:1886991/69‑1 (MQ=255) tGCCAGTCGAGCTGGTATTTCACCTGCTGTTTAGCACGGCGTAACAACGGCGTGTCGGTAATCGCATTg < 1:2104406/69‑1 (MQ=255) tGCCAGTCGAGCTGGTATTTCACCTGCTGTTTAGCACGGCGTAACAACGGCGTGTCGGTAATCGCATTg < 1:2109679/69‑1 (MQ=255) tGCCAGTCGAGCTGGTATTTCACCTGCTGTTTAGCACGGCGTAACAACGGCGTGTCGGTAATCGCATTg < 1:2206486/69‑1 (MQ=255) tGCCAGTCGAGCTGGTATTTCACCTGCTGTTTAGCACGGCGTAACAACGGCGTGTCGGTAATCGCATTg < 1:292776/69‑1 (MQ=255) tGCCAGTCGAGCTGGTATTTCACCTGCTGTTTAGCACGGCGTAACAACGGCGTGTCGGTAATCGCATTg < 1:1396328/69‑1 (MQ=255) tGCCAGTCGAGCTGGTATTTCACCTGCTGTTTAGCACGGCGTAACAACGGCGTGTCGGTAATCGCATTg < 1:1352377/69‑1 (MQ=255) tGCCAGTCGAGCTGGTATTTCACCTGCTGTTTAGCACGGCGTAACAACGGCGTGTCGGTAATCGCATTg < 1:3109671/69‑1 (MQ=255) aGTCGAGCTGGTATTTCACCTGCTGTTTAGCACGGCGTAACAACGGCGTGTCGGTAATCGCATTgcgcg < 1:830028/69‑1 (MQ=255) aGTCGAGCTGGTATTTCACCTGCTGTTTAGCACGGCGTAACAACGGCGTGTCGGTAATCGCATTgcgcg < 1:2023619/69‑1 (MQ=255) aGTCGAGCTGGTATTTCACCTGCTGTTTAGCACGGCGTAACAACGGCGTGTCGGTAATCGCATTgcgcg < 1:3249442/69‑1 (MQ=255) aGTCGAGCTGGTATTTCACCTGCTGTTTAGCACGGCGTAACAACGGCGTGTCGGTAATCGCATTgcgcg < 1:939625/69‑1 (MQ=255) aGTCGAGCTGGTATTTCACCTGCTGTTTAGCACGGCGTAACAACGGCGTGTCGGTAATCGCATTgcgcg < 1:1493436/69‑1 (MQ=255) aGTCGAGCTGGTATTTCACCTGCTGTTTAGCACGGCGTAACAACGGCGTGTCGGTAATCGCATTgcgcg < 1:1139697/69‑1 (MQ=255) gTCGAGCTGGTATTTCACCTGCTGTTTAGCACGGCGTAACAACGGCGTGTCGGTAATCGCATTgcgcgc < 1:1350429/69‑1 (MQ=255) gTCGAGCTGGTATTTCACCTGCTGTTTAGCACGGCGTAACAACGGCGTGTCGGTAATCGCATTgcgcgc < 1:214806/69‑1 (MQ=255) tCGAGCTGGTATTTCACCTGCTGTTTAGCACGGCGTAACAACGGCGTGTCGGTAATCGCATTGCGCGCa < 1:1522972/69‑1 (MQ=255) tCGAGCTGGTATTTCACCTGCTGTTTAGCACGGCGTAACAACGGCGTGTCAGTAATCGCATTGCGCGCa < 1:101444/69‑1 (MQ=255) tCGAGCTGGTATTTCACCTGCTGTTTAGCACGGCGTAACAACGGCGTGTCAGTAATCGCATTGCGCGCa < 1:315992/69‑1 (MQ=255) tCGAGCTGGTATTTCACCTGCTGTTTAGCACGGCGTAACAACGGCGTGTCAGTAATCGCATTGCGCGCa < 1:329370/69‑1 (MQ=255) tCGAGCTGGTATTTCACCTGCTGTTTAGCACGGCGTAACAACGGCGTGTCAGTAATCGCATTGCGCGCa < 1:2321313/69‑1 (MQ=255) tCGAGCTGGTATTTCACCTGCTGTTTAGCACGGCGTAACAACGGCGTGTCAGTAATCGCATTGCGCGCa < 1:2291887/69‑1 (MQ=255) cGAGCTGGTATTTCACCTGCTGTTTAGCACGGCGTAACAACGGCGTGTCGGTAATCGCATTGCGCGCa > 1:2800587/1‑68 (MQ=255) tCACCTGCTGTTTAGCACGGCGTAACAACGGCGTGTCGGTAATCGCATTGCGCGCAt > 1:1758437/1‑57 (MQ=255) tCACCTGCTGTTTAGCACGGCGTAACAACGGCGTGTCGGTAATCGCATTGCGCGCATCGACATAATCAt < 1:2052307/69‑1 (MQ=255) tCACCTGCTGTTTAGCACGGCGTAACAACGGCGTGTCGGTAATCGCATTGCGCGCATCGACATAATCAt < 1:2028874/69‑1 (MQ=255) tCACCTGCTGTTTAGCACGGCGTAACAACGGCGTGTCGGTAATCGCATTGCGCGCATCGACATAATCAt > 1:1296681/1‑69 (MQ=255) tCACCTGCTGTTTAGCACGGCGTAACAACGGCGTGTCGGTAATCGCATTGCGCGCATCGACATAATCAt > 1:2344246/1‑69 (MQ=255) tCACCTGCTGTTTAGCACGGCGTAACAACGGCGTGTCGGTAATCGCATTGCGCGCATCGACATAATCAt > 1:525660/1‑69 (MQ=255) tCACCTGCTGTTTAGCACGGCGTAACAACGGCGTGTCGGTAATCGCATTGCGCGCATCGACATAATCAt > 1:2384256/1‑69 (MQ=255) tCACCTGCTGTTTAGCACGGCGTAACAACGGCGTGTCGGTAATCGCATTGCGCGCATCGACATAATCAt > 1:3264115/1‑69 (MQ=255) tCACCTGCTGTTTAGCACGGCGTAACAACGGCGTGTCGGTAATCGCATTGCGCGCATCGACATAATCAt > 1:404290/1‑69 (MQ=255) tCACCTGCTGTTTAGCACGGCGTAACAACGGCGTGTCGGTAATCGCATTGCGCGCATCGACATAATCAt > 1:83647/1‑69 (MQ=255) tCACCTGCTGTTTAGCACGGCGTAACAACGGCGTGTCGGTAATCGCATTGCGCGCATCGACATAATCAt > 1:2699858/1‑69 (MQ=255) tCACCTGCTGTTTAGCACGGCGTAACAACGGCGTGTCGGTAATCGCATTGCGCGCATCGACATAATCAt > 1:2588763/1‑69 (MQ=255) tAGGACGGCGTAACAACGGCGTGTCGGTAATCGCATTGCGCGCATCGACATAATCATAACTCACAGTa < 1:353530/68‑1 (MQ=255) tAGCACGGCGTAACAACGGCGTGTCGGTAATCGCATTGCGCGCATCGACATAATCATAACTCACAGTa < 1:289814/68‑1 (MQ=255) tAGCACGGCGTAACAACGGCGTGTCGGTAATCGCATTGCGCGCATCGACATAATCATAACTCACAGTa < 1:2898079/68‑1 (MQ=255) tAGCACGGCGTAACAACGGCGTGTCGGTAATCGCATTGCGCGCATCGACATAATCATAACTCACAGTAt > 1:2899249/1‑69 (MQ=255) tAGCACGGCGTAACAACGGCGTGTCAGTAATCGCAt > 1:1898663/1‑36 (MQ=255) tAGCACGGCGTAACAACGGCGTGTCAGTAATCGCATTGCGCGCATCAACATAATCATa > 1:3156100/1‑58 (MQ=255) tAGCACGGCGTAACAACGGCGTGTCAGTAATCGCATTGCGCGCATCAACATAATCATAACTCACAGTa > 1:1075474/1‑68 (MQ=255) tAGCACGGCGTAACAACGGCGTGTCAGTAATCGCATTGCGCGCATCAACATAATCATAACTCACAGTAt > 1:1958834/1‑69 (MQ=255) tAGCACGGCGTAACAACGGCGTGTCAGTAATCGCATTGCGCGCATCAACATAATCATAACTCACAGTAt > 1:3109617/1‑69 (MQ=255) tAGCACGGCGTAACAACGGCGTGTCAGTAATCGCATTGCGCGCATCAACATAATCATAACTCACAGTAt > 1:3119990/1‑69 (MQ=255) tAGCACGGCGTAACAACGGCGTGTCAGTAATCGCATTGCGCGCATCAACATAATCATAACTCACAGTAt > 1:41867/1‑69 (MQ=255) tAGCACGGCGTAACAACGGCGTGTCAGTAATCGCATTGCGCGCATCAACATAATCATAACTCACAGTAt > 1:567161/1‑69 (MQ=255) tAGCACGGCGTAACAACGGCGTGTCAGTAATCGCATTGCGCGCATCAACATAATCATAACTCACAGTAt > 1:1178652/1‑69 (MQ=255) tAGCACGGCGTAACAACGGCGTGTCAGTAATCGCATTGCGCGCATCAACATAATCATAACTCACAGTAt > 1:764050/1‑69 (MQ=255) tAGCACGGCGTAACAACGGCGTGTCAGTAATCGCATTGCGCGCATCAACATAATCATAACTCACAGTAt > 1:845673/1‑69 (MQ=255) tAGCACGGCGTAACAACGGCGTGTCAGTAATCGCATTGCGCGCATCAACATAATCATAACTCACAGTAt > 1:1694076/1‑69 (MQ=255) tAGCACGGCGTAACAACGGCGTGTCAGTAATCGCATTGCGCGCATCAACATAATCATAACTCACAGTAt > 1:2823901/1‑69 (MQ=255) tAGCACGGCGTAACAACGGCGTGTCAGTAATCGCATTGCGCGCATCAACATAATCATAACTCACAGTAt > 1:2802330/1‑69 (MQ=255) tAGCACGGCGTAACAACGGCGTGTCAGTAATCGCATTGCGCGCATCAACATAATCATAACTCACAGTAt > 1:1668006/1‑69 (MQ=255) tAGCACGGCGTAACAACGGCGTGTCAGTAATCGCATTGCGCGCATCAACATAATCATAACTCACAGTAt > 1:2335862/1‑69 (MQ=255) tAGCACGGCGTAACAACGGCGTGTCAGTAATCGCATTGCGCGCATCAACATAATCATAACTCACAGTAt > 1:2400802/1‑69 (MQ=255) tAGCACGGCGTAACAACGGCGTGTCAGTAATCGCATTGCGCGCATCAACATAATCATAACTCACAGTAt > 1:1598713/1‑69 (MQ=255) tAGCACGGCGTAACAACGGCGTGTCAGTAATCGCATTGCGAGCATCAACATAATCATAACTCACAGTAt > 1:358307/1‑69 (MQ=255) tAGCACGGCGTAACAACGGCGTGTCAGCAATCGCATTGCGCGCATCAACATAATCATAACTCACAGTAt > 1:1486536/1‑69 (MQ=255) gCACGGCGTAACAACGGCGTGTCGGTAATCGCATTGCGCGCATCGACATAATCATAACTCACAGTATg < 1:3312348/68‑1 (MQ=255) gCACGGCGTAACAACGGCGTGTCGGTAATCGCATTGCGCGCATCGACATAATCATAACTCACAGTATg < 1:1088349/68‑1 (MQ=255) gCACGGCGTAACAACGGCGTGTCGGTAATCGCATTGCGCGCATCGACATAATCATAACTCACAGTATg < 1:3276677/68‑1 (MQ=255) gCACGGCGTAACAACGGCGTGTCGGTAATCGCATTGCGCGCATCGACATAATCATAACTCACAGTATg < 1:2369958/68‑1 (MQ=255) acGGCGTGTCGGTAATCGCATTGCGCGCATCGACATAATCATAACTCACAg > 1:120738/1‑51 (MQ=255) acGGCGTGTCGGTAATCGCATTGCGCGCATCGACATAATCATAACTCACAg > 1:1921685/1‑51 (MQ=255) acGGCGTGTCGGTAATCGCATTGCGCGCATCGACATAATCATAACTCACAg > 1:923558/1‑51 (MQ=255) acGGCGTGTCGGTAATCGCATTGCGCGCATCGACATAATCATAACTCACAg > 1:1699369/1‑51 (MQ=255) acGGCGTGTCGGTAATCGCATTGCGCGCATCGACATAATCATAACTCACAg > 1:3102069/1‑51 (MQ=255) acGGCGTGTCGGTAATCGCATTGCGCGCATCGACATAATCATAACTCACAg > 1:2764155/1‑51 (MQ=255) acGGCGTGTCGGTAATCGCATTGCGCGCATCGACATAATCATAACTCACAg > 1:2382041/1‑51 (MQ=255) acGGCGTGTCGGTAATCGCATTGCGCGCATCGACATAATCATAACTCACAg > 1:2713169/1‑51 (MQ=255) acGGCGTGTCGGTAATCGCATTGCGCGCATCGACATAATCATAACTCACAg > 1:2532475/1‑51 (MQ=255) acGGCGTGTCGGTAATCGCATTGCGCGCATCGACATAATCATAACTCACAg > 1:1218428/1‑51 (MQ=255) acGGCGTGTCGGTAATCGCATTGCGCGCATCGACATAATCATAACTCACAg > 1:1252893/1‑51 (MQ=255) acGGCGTGTCGGTAATCGCATTGCGCGCATCGACATAATCATAACTCACAg > 1:3308872/1‑51 (MQ=255) acGGCGTGTCGGTAATCGCATTGCGCGCATCGACATAATCATAACTCACAGTATGCGTCAGTGGTCCgg < 1:1276787/69‑1 (MQ=255) acGGCGTGTCGGTAATCGCATTGCGCGCATCGACATAATCATAACTCACAGTATGCGTCAGTGGTCCgg < 1:1500561/69‑1 (MQ=255) acGGCGTGTCGGTAATCGCATTGCGCGCATCGACATAATCATAACTCACAGTATGCGTCAGTGGTCCgg < 1:3214370/69‑1 (MQ=255) acGGCGTGTCGGTAATCGCATTGCGCGCATCGACATAATCATAACTCACAGTATGCGTCAGTGGTCCgg < 1:390335/69‑1 (MQ=255) acGGCGTGTCGGTAATCGCATTGCGCGCATCGACATAATCATAACTCACAGTATGCGTCAGTGGTCCgg < 1:1818969/69‑1 (MQ=255) acGGCGTGTCGGTAATCGCATTGCGCGCATCGACATAATCATAACTCACAGTATGCGTCAGTGGTCCgg < 1:3319575/69‑1 (MQ=255) cGTGTCGGTAATCGCATTGCGCGCATCGACATAATCATAACTCACAGTATGCGTCAGTGGTCCgg < 1:2014310/65‑1 (MQ=255) | ATACAACTGCCAGTCGAGCTGGTATTTCACCTGCTGTTTAGCACGGCGTAACAACGGCGTGTCGGTAATCGCATTGCGCGCATCGACATAATCATAACTCACAGTATGCGTCAGTGGTCCGG > minE/2277153‑2277274 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |