| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,277,534 | G→A | 23.7% | F405F (TTC→TTT) | btuB ← | vitamin B12/cobalamin outer membrane transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,277,534 | 0 | G | A | 23.7% | 86.7 / 19.7 | 67 | F405F (TTC→TTT) | btuB | vitamin B12/cobalamin outer membrane transporter |
| Reads supporting (aligned to +/- strand): ref base G (25/26); new base A (5/11); total (30/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.58e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.29e-01 | |||||||||||
TTCCGTAGAAGCCATACAGTTGCCCCAGATTTGGTGCCTTATAAGATGTCCCGTAGGAAGCAATGAAGCGATAACCTTCGATGAATTCCCAACCGGCGCTGGTTTGCCAGGTTCCAT > minE/2277470‑2277586 | ttCCGTAGAAGCCATACAGTTGCCCCAGATTTGGTGCCTTATAAGATGTCCCGTAGGAAGCAATGAAGc < 1:3027767/69‑1 (MQ=255)ttCCGTAGAAGCCATACAGTTGCCCCAGATTTGGTGCCTTATAAGATGTCCCGTAGGAAGCAATGAAGc < 1:2817289/69‑1 (MQ=255)ttCCGTAGAAGCCATACAGTTGCCCCAGATTTGGTGCCTTATAAGATGTCCCGTAGGAAGCAATGAAGc < 1:2283053/69‑1 (MQ=255) tCCGTAGAAGCCATACAGTTGCCCCAGATTTGGTGCCTTATAAGATGTCCCGTAGGAAGCAATGAAGc < 1:3034139/68‑1 (MQ=255) tAGAAGCCATACAGTTGCCCCAGATTTTGTGCCTTATAAGATGTCCCGTAGGAAGCAATGAAGCGATa < 1:3056488/68‑1 (MQ=255) tAGAAGCCATACAGTTGCCCCAGATTTGGTGCCTTATAAGATGTCCCGTAGGAAGCAATGAAGCGATa < 1:1025027/68‑1 (MQ=255) tAGAAGCCATACAGTTGCCCCAGATTTGGTGCCTTATAAGATGTCCCGTAGGAAGCAATGAAGCGATa < 1:348678/68‑1 (MQ=255) tAGAAGCCATACAGTTGCCCCAGATTTGGTGCCTTATAAGATGTCCCGTAGGAAGCAATGAAGCGATa < 1:2804215/68‑1 (MQ=255) tAGAAGCCATACAGTTGCCCCAGATTTGGTGCCTTATAAGATGTCCCGTAGGAAGCAATGAAGCGATa < 1:2275669/68‑1 (MQ=255) tAGAAGCCATACAGTTGCCCCAGATTTGGTGCCTTATAAGATGTCCCGTAGGAAGCAATGAAGCGATa < 1:1953601/68‑1 (MQ=255) tAGAAGCCATACAGTTGCCCCAGATTTGGTGCCTTATAAGATGTCCCGTAGGAAGCAATGAAGCGATa < 1:160222/68‑1 (MQ=255) tAGAAGCCATACAGTTGCCCCAGATTTGGTGCCTTATAAGATGTCCCGTAGGAAGCAATGAAGCGATa < 1:1400082/68‑1 (MQ=255) gAAGCCATACAGTTGCCCCAGATTTGGTGCCTTATAAGATGTCCCGTAGGAAGCAATGAAGCGATa < 1:2031723/66‑1 (MQ=255) ccATACAGTTGCCCCAGATTTGGTGCCTTATAAGATGTCCCGTAGGAAGCAATGAAGCGATAACCTTc < 1:795379/68‑1 (MQ=255) cATACAGTTGCCCCAGATTTGGTGCCTTATAAGATGTCCCGTAGGAAGCAATGAAGCGATAACCTTCg > 1:2643005/1‑68 (MQ=255) cATACAGTTGCCCCAGATTTGGTGCCTTATAAGATGTCCCGTAGGAAGCAATGAAGCGATAACCTTCg > 1:2700833/1‑68 (MQ=255) cATACAGTTGCCCCAGATTTGGTGCCTTATAAGATGTCCCGTAGGAAGCAATGAAGCGATAACCTTCg > 1:249640/1‑68 (MQ=255) cATACAGTTGCCCCAGATTTGGTGCCTTATAAGATGTCCCGTAGGAAGCAATGAAGCGATAACCTTCg > 1:416305/1‑68 (MQ=255) cATACAGTTGCCCCAGATTTGGTGCCTTATAAGATGTCCCGTAGGAAGCAATGAAGCGATAACCTTCg > 1:1318286/1‑68 (MQ=255) cAGTTGCCCCAGATTTGGTGCCTTATAAGATGTCCCGTAGGAAGCAATGAAGGGATa > 1:1224395/1‑57 (MQ=255) cAGTTGCCCCAGATTTGGTGCCTTATAAGATGTCCCGTAGGAAGCAATGAAGCGAt > 1:787988/1‑56 (MQ=255) cAGTTGCCCCAGATTTGGTGCCTTATAAGATGTCCCGTAGGAAGCAATGAAGCGATa > 1:1687879/1‑57 (MQ=255) cAGTTGCCCCAGATTTGGTGCCTTATAAGATGTCCCGTAGGAAGCAATGAAGCGATa > 1:2976328/1‑57 (MQ=255) cAGTTGCCCCAGATTTGGTGCCTTATAAGATGTCCCGTAGGAAGCAATGAAGCGATa < 1:1850190/57‑1 (MQ=255) cAGTTGCCCCAGATTTGGTGCCTTATAAGATGTCCCGTAGGAAGCAATGAAGCGATa > 1:1537269/1‑57 (MQ=255) cAGTTGCCCCAGATTTGGTGCCTTATAAGATGTCCCGTAGGAAGCAATGAAGCGATa < 1:2296288/57‑1 (MQ=255) cAGTTGCCCCAGATTTGGTGCCTTATAAGATGTCCCGTAGGAAGCAATGAAGCGATa > 1:3063383/1‑57 (MQ=255) cAGTTGCCCCAGATTTGGTGCCTTATAAGATGTCCCGTAGGAAGCAATGAAGCGATa > 1:2432025/1‑57 (MQ=255) cAGTTGCCCCAGATTTGGTGCCTTATAAGATGTCCCGTAGGAAGCAATGAAGCGATa > 1:2435945/1‑57 (MQ=255) cAGTTGCCCCAGATTTGGTGCCTTATAAGATGTCCCGTAGGAAGCAATGAAGCGATAACCTTCGATGaa > 1:2067452/1‑69 (MQ=255) cAGTTGCCCCAGATTTGGTGCCTTATAAGATGTCCCGTAGGAAGCAATGAAGCGATAACCTTCGATGaa > 1:564312/1‑69 (MQ=255) cAGTTGCCCCAGATTTGGTGCCTTATAAGATGTCCCGTAGGAAGCAATGAAGCGATAACCTTCGATGaa > 1:721936/1‑69 (MQ=255) cAGTTGCCCCAGATTTGGTGCCTTATAAGATGTCCCGTAGGAAGCAATGAAGCGATAACCTTCGATGaa > 1:1344595/1‑69 (MQ=255) cAGTTGCCCCAGATTTGGTGCCTTATAAGATGTCCCGTAGGAAGCAATGAAGCGATAACCTTCGATGaa > 1:2135395/1‑69 (MQ=255) gCCCCAGATTTGGTGCCTTATAAGATGTCCCGTAGGAAGCAATGAAGCGATAACCTTCGATGAATTccc < 1:1478500/69‑1 (MQ=255) cccAGATTTGGTGCCTTATAAGATGTCCCGTAGGAAGCAATGAAGCGATAACCTTCGATGAATTCCCa < 1:858752/68‑1 (MQ=255) cccAGATTTGGTGCCTTATAAGATGTCCCGTAGGAAGCAATGAAGCGATAACCTTCGATGAATTCCCa < 1:2651555/68‑1 (MQ=255) cccAGATTTGGTGCCTTATAAGATGTCCCGTAGGAAGCAATGAAGCGATAACCTTCGATGAATTCCCa < 1:3296656/68‑1 (MQ=255) cccAGATTTGGTGCCTTATAAGATGTCCCGTAGGAAGCAATGAAGCGATAACCTTCGATGAATTCCCa < 1:2339538/68‑1 (MQ=255) aaGATTTGGTGCCTTATAAGATGTTCCGTAGGAAGCAATAAAGCGATa > 1:2193694/2‑48 (MQ=255) aaGATTTGGTGCCTTATAAGATGTTCCGTAGGAAGCAATAAAGCGATAACCTTCGATGAACTCCCAAcc > 1:2737387/2‑69 (MQ=255) aaGATTTGGTGCCTTATAAGATGTTCCGTAGGAAGCAATAAAGCGATAACCTTCGATGAACTCCCAAcc > 1:298350/2‑69 (MQ=255) aaGATTTGGTGCCTTATAAGATGTTCCGTAGGAAGCAATAAAGCGATAACCTTCGATGAACTCCCAAcc > 1:1842369/2‑69 (MQ=255) aaGATTTGGTGCCTTATAAGATGTTCCGTAGGAAGCAATAAAGCGATAACCTTCGATGAACTCCCAAc > 1:370574/2‑68 (MQ=255) ttGGTGCCTTATAAGATGTCCCGTAGGAAGCAATGAAGCGATAACCTTCGATGAATTCCCAACCGgcgc > 1:2459959/1‑69 (MQ=255) aTGTCCCGTAGGAAGCAATGAAGCGATAACCTTCGATGAATTCCCAACCGGCGCTGGTTTGCCAGGTTc > 1:558927/1‑69 (MQ=255) aTGTCCCGTAGGAAGCAATGAAGCGATAACCTTCGATGAATTCCCAACCGGCGCTGGTTTGCCAGGTTc > 1:2550440/1‑69 (MQ=255) aTGTCCCGTAGGAAGCAATGAAGCGATAACCTTCGATGAATTCCCAACCGGCGCTGGTTTGCCAGGTTc > 1:2251240/1‑69 (MQ=255) aTGTCCCGTAGGAAGCAATGAAGCGATAACCTTCGATGAATTCCCAACCGGCGCTGGTTTGCCAGGTTc > 1:60552/1‑69 (MQ=255) aTGTCCCGTAGGAAGCAATGAAGCGATAACCTTCGATGAATTCCCAACCGGCGCTGGTTTGCCAGGTTc > 1:2179826/1‑69 (MQ=255) aTGTCCCGTAGGAAGCAATGAAGCGATAACCTTCGATGAATTCCCAACCGGCGCTGGTTTGCCAGGTTc > 1:1599941/1‑69 (MQ=255) gttccGTAGGAAGCAATAAAGCGATAACCTTCGATGAACTCCCAACCGGCGCTGGTTTGc < 1:637438/57‑1 (MQ=255) gttccGTAGGAAGCAATAAAGCGATAACCTTCGATGAACTCCCAACCGGCGCTGGTTTGc < 1:902010/57‑1 (MQ=255) gttccGTAGGAAGCAATAAAGCGATAACCTTCGATGAACTCCCAACCGGCGCTGGTTTGc < 1:307051/57‑1 (MQ=255) gttccGTAGGAAGCAATAAAGCGATAACCTTCGATGAACTCCCAACCGGCGCTGGTTTGc < 1:2993824/57‑1 (MQ=255) gttccGTAGGAAGCAATAAAGCGATAACCTTCGATGAACTCCCAACCGGCGCTGGTTTGc < 1:2958202/57‑1 (MQ=255) gttccGTAGGAAGCAATAAAGCGATAACCTTCGATGAACTCCCAACCGGCGCTGGTTTGc < 1:2607605/57‑1 (MQ=255) gttccGTAGGAAGCAATAAAGCGATAACCTTCGATGAACTCCCAACCGGCGCTGGTTTGc < 1:2551495/57‑1 (MQ=255) gttccGTAGGAAGCAATAAAGCGATAACCTTCGATGAACTCCCAACCGGCGCTGGTTTGc < 1:1829107/57‑1 (MQ=255) gttccGTAGGAAGCAATAAAGCGATAACCTTCGATGAACTCCCAACCGGCGCTGGTTTGc < 1:1797997/57‑1 (MQ=255) gttccGTAGGAAGCAATAAAGCGATAACCTTCGATGAACTCCCAACCGGCGCTGGTTTGc < 1:1599714/57‑1 (MQ=255) gttccGTAGGAAGCAATAAAGCGATAACCTTCGATGAACTCCCAACCAGCGCTGGTTTGc < 1:15644/57‑1 (MQ=255) tCCCGTAGGAAGCAATGAAGCGATAACCTTCGATTAATTCCCAACCGGCGCTGGTTTGCCAGGTTCCAt < 1:657398/69‑1 (MQ=255) aGCAATGAAGCGATAACCTTCGATGAATTCCCAACCGGCGCTGGTTTGCCAGGTTc < 1:502965/56‑1 (MQ=255) aGCAATGAAGCGATAACCTTCGATGAATTCCCAACCGGCGCTGGTTTGCCAGGTTc < 1:218826/56‑1 (MQ=255) aGCAATGAAGCGATAACCTTCGATGAATTCCCAACCGGCGCTGGTTTGCCAGGTTc < 1:1337766/56‑1 (MQ=255) aGCAATGAAGCGATAACCTTCGATGAATTCCCAACCGGCGCTGGTTTGCCAGGTTc < 1:1135569/56‑1 (MQ=255) | TTCCGTAGAAGCCATACAGTTGCCCCAGATTTGGTGCCTTATAAGATGTCCCGTAGGAAGCAATGAAGCGATAACCTTCGATGAATTCCCAACCGGCGCTGGTTTGCCAGGTTCCAT > minE/2277470‑2277586 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |