| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,277,603 | G→A | 42.6% | N382N (AAC→AAT) | btuB ← | vitamin B12/cobalamin outer membrane transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,277,603 | 0 | G | A | 42.6% | 22.7 / 48.8 | 68 | N382N (AAC→AAT) | btuB | vitamin B12/cobalamin outer membrane transporter |
| Reads supporting (aligned to +/- strand): ref base G (11/28); new base A (7/22); total (18/50) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.86e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCGATAACCTTCGATGAATTCCCAACCGGCGCTGGTTTGCCAGGTTCCATGACGACCAAACTGTGAGTTATCGTCACTGCGTGCTGCGCCTTCAAAGGTAAAATCGCCGACTTGTTGCAGCCCGGTCAGATAGA > minE/2277537‑2277670 | gCGATAACCTTCGATGAATTCCCAACCGGCGCTGGTTTGCCAGGTTCCATGACGACCAAACTGTGAGtt < 1:1156755/69‑1 (MQ=255)gCGATAACCTTCGATGAATTCCCAACCGGCGCTGGTTTGCCAGGTTCCATGACGACCAAACTGTGAGtt < 1:2910390/69‑1 (MQ=255) aTAACCTTCGATGAATTCCCAACCGGCTCTGGTTTGCCAGGTTCCATGACGACCAAACTGTGAGTTATc > 1:1156907/1‑69 (MQ=255) aTAACCTTCGATGAATTCCCAACCGGCGCTGGTTTGCCAGGTTCCATGACGACCAAACTGTGAGtt < 1:1718846/66‑1 (MQ=255) aTAACCTTCGATGAATTCCCAACCGGCGCTGGTTTGCCAGGTTCCATGACGACCAAACTGTGAGTTATc > 1:2518156/1‑69 (MQ=255) aTAACCTTCGATGAATTCCCAACCGGCGCTGGTTTGCCAGGTTCCATGACGACCAAACTGTGAGTTATc < 1:2015337/69‑1 (MQ=255) taCCTTCGATGAATTCCCAACCGGCGCTGGTTTGCCAGGTTCCATGACGACCAAACTGTGAGTTATCGt < 1:175132/68‑1 (MQ=255) aaCCTTCGATGAATTCCCAACCGGCGCTGGTTTGCCAGGTTCCATGACGACCAAACTGTGAGTTATCGt < 1:2322560/69‑1 (MQ=255) aaCCTTCGATGAATTCCCAACCGGCGCTGGTTTGCCAGGTTCCATGACGACCAAACTGTGAGTTATCGt > 1:1208041/1‑69 (MQ=255) aaCCTTCGATGAATTCCCAACCGGCGCTGGTTTGCCAGGTTCCATGACGACCAAACTGTGAGTTATCGt < 1:96220/69‑1 (MQ=255) aaCCTTCGATGAATTCCCAACCGGCGCTGGTTTGCCAGGTTCCATGACGACCAAACTGTGAGTTATCGt < 1:189982/69‑1 (MQ=255) aaCCTTCGATGAATTCCCAACCGGCGCTGGTTTGCCAGGTTCCATGACGACCAAACTGTGAGTTATCGt < 1:3136614/69‑1 (MQ=255) aaCCTTCGATGAATTCCCAACCGGCGCTGGTTTGCCAGGTTCCATGACGACCAAACTGTGAGTTATCGt < 1:2272650/69‑1 (MQ=255) aaCCTTCGATGAATTCCCAACCGGCGCTGGTTTGCCAGGTTCCATGACGACCAAACTGTGAGTTATCGt < 1:1822995/69‑1 (MQ=255) aaCCTTCGATGAATTCCCAACCGGCGCTGGTTTGCCAGGTTCCATGACGACCAAACTGTGAGTTATCGt < 1:176609/69‑1 (MQ=255) aaCCTTCGATGAATTCCCAACCGGCGCTGGTTTGCCAGGTTCCATGACGACCAAACTGTGAGTTATCGt < 1:376700/69‑1 (MQ=255) aaCCTTCGATGAATTCCCAACCGGCGCTGGTTTGCCAGGTTCCATGACGACCAAACTGTGAGTTATCGt < 1:1692704/69‑1 (MQ=255) aaCCTTCGATGAATTCCCAACCGGCGCTGGTTTGCCAGGTTCCATGACGACCAAACTGTGAGTTATCGt < 1:163970/69‑1 (MQ=255) aaCCTTCGATGAATTCCCAACCGGCGCTGGTTTGCCAGGTTCCATGACGACCAAACTGTGAGTTATCGt < 1:49696/69‑1 (MQ=255) aaCCTTCGATGAATTCCCAACCGGCGCTGGTTTGCCAGGTTCCATGACGACCAAACTGTGAGTTATCGt < 1:515162/69‑1 (MQ=255) aaCCTTCGATGAATTCCCAACCGGCGCTGGTTTGCCAGGTTCCATGACGACCAAACTGTGAGTTATCGt > 1:636213/1‑69 (MQ=255) aaCCTTCGATGAATTCCCAACCGGCGCTGGTTTGCCAGGTTCCATGACGACCAAACTGTGAGTTATCGt < 1:747021/69‑1 (MQ=255) aCCTTCGATGAATTCCCAACCGGCGCTGGTTTGCCAGGTTCCATGACGACCAAACTGTGAGTTATCGTc > 1:1841791/1‑69 (MQ=255) aCCTTCGATGAATTCCCAACCGGCGCTGGTTTGCCAGGTTCCATGACGACCAAACTGTGAGTTATCGTc > 1:807695/1‑69 (MQ=255) aCCTTCGATGAATTCCCAACCGGCGCTGGTTTGCCAGGTTCCATGACGACCAAACTGTGAGTTATCGTc > 1:2017643/1‑69 (MQ=255) gTTTGCCAGGTACCATGACGACCAAACTGAGAATTATCGTCGCTGCg < 1:2677232/47‑1 (MQ=255) gTTTGCCAGGTACCATGACGACCAAACTGAGAATTATCGTCGCTGCg < 1:994301/47‑1 (MQ=255) gTTTGCCAGGTACCATGACGACCAAACTGAGAATTATCGTCGCTGCg < 1:2675729/47‑1 (MQ=255) gTTTGCCAGGTACCATGACGACCAAACTGAGAATTATCGTCGCTGCg < 1:2805733/47‑1 (MQ=255) gTTTGCCAGGTACCATGACGACCAAACTGAGAATTATCGTCGCTGCg < 1:2830014/47‑1 (MQ=255) gTTTGCCAGGTACCATGACGACCAAACTGAGAATTATCGTCGCTGCg < 1:315295/47‑1 (MQ=255) gTTTGCCAGGTACCATGACGACCAAACTGAGAATTATCGTCGCTGCg < 1:3189382/47‑1 (MQ=255) gTTTGCCAGGTACCATGACGACCAAACTGAGAATTATCGTCGCTGCg < 1:2464005/47‑1 (MQ=255) gTTTGCCAGGTACCATGACGACCAAACTGAGAATTATCGTCGCTGCg < 1:835301/47‑1 (MQ=255) gTTTGCCAGGTACCATGACGACCAAACTGAGAATTATCGTCGCTGCg < 1:407345/47‑1 (MQ=255) gTTTGCCAGGTACCATGACGACCAAACTGAGAATTATCGTCGCTGCg < 1:621038/47‑1 (MQ=255) gTTTGCCAGGTACCATGACGACCAAACTGAGAATTATCGTCGCTGCg < 1:1224927/47‑1 (MQ=255) gTTTGCCAGGTACCATGACGACCAAACTGAGAATTATCGTCGCTGCg < 1:1234133/47‑1 (MQ=255) gTTTGCCAGGTACCATGACGACCAAACTGAGAATTATCGTCGCTGCg < 1:220802/47‑1 (MQ=255) gTTTGCCAGGTACCATGACGACCAAACTGAGAATTATCGTCGCTGCg < 1:2136951/47‑1 (MQ=255) gTTTGCCAGGTACCATGACGACCAAACTGAGAATTATCGTCGCTGCg < 1:1899679/47‑1 (MQ=255) gTTTGCCAGGTACCATGACGACCAAACTGAGAATTATCGTCGCTGCg < 1:1302995/47‑1 (MQ=255) gTTTGCCAGGTACCATGACGACCAAACTGAGAATTATCGTCGCTGCg < 1:1394548/47‑1 (MQ=255) gTTTGCCAGGTACCATGACGACCAAACTGAGAATTATCGTCGCTGCg < 1:142466/47‑1 (MQ=255) gTTTGCCAGGTACCATGACGACCAAACTGAGAATTATCGTCGCTGCg < 1:1471330/47‑1 (MQ=255) gTTTGCCAGGTACCATGACGACCAAACTGAGAATTATCGTCGCTGCg < 1:1518640/47‑1 (MQ=255) ttCCATGACGACCAAACTGTGAGTTATCGTCACTGCGTGCTGCGCCTTCAAAGGTAAAATCGCCGACtt < 1:835398/69‑1 (MQ=255) tCCATGACGACCAAACTGTGAGTTATCGTCACTGCGTGCTGCGCCTTCAAAGGTAAAATCGCCGACttg > 1:1716949/1‑69 (MQ=255) tCCATGACGACCAAACTGTGAGTTATCGTCACTGCGTGCTGCGCCTTCAAAGGTAAAATCGCCGACtt < 1:361020/68‑1 (MQ=255) ccATGACGACCAAACTGAGAATTATCTTCGCTGCg < 1:510142/35‑1 (MQ=19) acgacCAAACTGTGAGTTATCGTCACTGCGTGCTGCGCCTTCAAAGGTAAAATCGCCGACTTGTTGCAg > 1:2220327/1‑69 (MQ=255) cgacCAAACTGAGAATTATCGTCGCTGCGTCCTGCGCCTTCAAAAGTAAAATCGCCGACTTGCTGCAg > 1:2652026/1‑68 (MQ=255) cgacCAAACTGAGAATTATCGTCGCTGCGTCCTGCGCCTTCAAAAGTAAAATCGCCGACTTGCTGCAg > 1:442789/1‑68 (MQ=255) cgacCAAACTGAGAATTATCGTCGCTGCGTCCTGCGCCTTCAAAAGTAAAATCGCCGACTTGCTGCAg > 1:1140641/1‑68 (MQ=255) cgacCAAACTGAGAATTATCGTCGCTGCGTCCTGCGCCTTCAAAAGTAAAATCGCCGACTTGCTGCAg > 1:358266/1‑68 (MQ=255) cgacCAAACTGAGAATTATCGTCGCTGCGTCCTGCGCCTTCAAAAGTAAAATCGCCGACTTGCTGCAg > 1:1898742/1‑68 (MQ=255) cgacCAAACTGAGAATTATCGTCGCTGCGTCCTGCGCCTTCAAAAGTAAAATCGCCGACTCGCTGCAg > 1:387305/1‑68 (MQ=255) cgacCAAACTGAGAATTATCGTCGCAGCGTCCTGCGCCTTCAAAAGTAAAATCGCCGACTTGCTGCAg > 1:861381/1‑68 (MQ=255) aCTGTGAGTTATCGTCACTGCGTGCTGCGCCTTCAAAGGTAAAATCGCCGACTTGTTGCAGCCCGGTCa < 1:416026/69‑1 (MQ=255) aCTGTGAGTTATCGTCACTGCGTGCTGCGCCTTCAAAGGTAAAATCGCCGACTTGTTGCAGCCCGGTCa < 1:1543739/69‑1 (MQ=255) aCTGTGAGTTATCGTCACTGCGTGCTGCGCCTTCAAAGGTAAAATCGCCGACTTGTTGCAGCCCGGTCa < 1:323795/69‑1 (MQ=255) tgtgAGTTATCGTCACTGCGTGCTGCGCCTTCAAAGGTAAAATCGCCGACTTGTTGCAGCCCGGTCaga < 1:1669890/69‑1 (MQ=255) tgtgAGTTATCGTCACTGCGTGCTGCGCCTTCAAAGGTAAAATCGCCGACTTGTTGCAGCCCGGTCaga < 1:3300946/69‑1 (MQ=255) tgtgAGTTATCGTCACTGCGTGCTGCGCCTTCAAAGGTAAAATCGCCGACTTGTTGCAGCCCGGTCaga < 1:1835946/69‑1 (MQ=255) aGTTATCGTCACTGCTTGCTGCGCCTTCAAAGGTAAAATCGCCGACTTGTTGCAGCCCGGTCagata < 1:2651043/67‑1 (MQ=255) aGTTATCGTCACTGCGTGCTGCGCCTTCAAAGGTAAAATCGCCGACTTGTTGCAGCCCGGTCagataga > 1:2376560/1‑69 (MQ=255) aGTTATCGTCACTGCGTGCTGCGCCTTCAAAGGTAAAATCGCCGACTTGTTGCAGCCCGGTCagataga > 1:1108553/1‑69 (MQ=255) aGTTATCGTCACTGCGTGCTGCGCCTTCAAAGGTAAAATCGCCGACTTGTTGCAGCCCGGTCagata < 1:2777580/67‑1 (MQ=255) | GCGATAACCTTCGATGAATTCCCAACCGGCGCTGGTTTGCCAGGTTCCATGACGACCAAACTGTGAGTTATCGTCACTGCGTGCTGCGCCTTCAAAGGTAAAATCGCCGACTTGTTGCAGCCCGGTCAGATAGA > minE/2277537‑2277670 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |