| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,277,651 | T→C | 17.4% | Q366Q (CAA→CAG) | btuB ← | vitamin B12/cobalamin outer membrane transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,277,651 | 0 | T | C | 17.4% | 114.4 / 14.0 | 74 | Q366Q (CAA→CAG) | btuB | vitamin B12/cobalamin outer membrane transporter |
| Reads supporting (aligned to +/- strand): ref base T (36/25); new base C (7/6); total (43/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.65e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.54e-01 | |||||||||||
ACGACCAAACTGTGAGTTATCGTCACTGCGTGCTGCGCCTTCAAAGGTAAAATCGCCGACTTGTTGCAGCCCGGTCAGATAGATGCCGGTATTACGTTGATCATATCCATCCTCAACATAACCTG > minE/2277588‑2277712 | acgacCAAACTGTGAGTTATCGTCACTGCGTGCTGCGCCTTCAAAGGTAAAATCGCCGACTTGTTGCAg > 1:2220327/1‑69 (MQ=255) cgacCAAACTGAGAATTATCGTCGCTGCGTCCTGCGCCTTCAAAAGTAAAATCGCCGACTTGCTGCAg > 1:1898742/1‑68 (MQ=255) cgacCAAACTGAGAATTATCGTCGCTGCGTCCTGCGCCTTCAAAAGTAAAATCGCCGACTTGCTGCAg > 1:2652026/1‑68 (MQ=255) cgacCAAACTGAGAATTATCGTCGCTGCGTCCTGCGCCTTCAAAAGTAAAATCGCCGACTTGCTGCAg > 1:1140641/1‑68 (MQ=255) cgacCAAACTGAGAATTATCGTCGCTGCGTCCTGCGCCTTCAAAAGTAAAATCGCCGACTTGCTGCAg > 1:442789/1‑68 (MQ=255) cgacCAAACTGAGAATTATCGTCGCTGCGTCCTGCGCCTTCAAAAGTAAAATCGCCGACTTGCTGCAg > 1:358266/1‑68 (MQ=255) cgacCAAACTGAGAATTATCGTCGCTGCGTCCTGCGCCTTCAAAAGTAAAATCGCCGACTCGCTGCAg > 1:387305/1‑68 (MQ=255) cgacCAAACTGAGAATTATCGTCGCAGCGTCCTGCGCCTTCAAAAGTAAAATCGCCGACTTGCTGCAg > 1:861381/1‑68 (MQ=255) aCTGTGAGTTATCGTCACTGCGTGCTGCGCCTTCAAAGGTAAAATCGCCGACTTGTTGCAGCCCGGTCa < 1:323795/69‑1 (MQ=255) aCTGTGAGTTATCGTCACTGCGTGCTGCGCCTTCAAAGGTAAAATCGCCGACTTGTTGCAGCCCGGTCa < 1:416026/69‑1 (MQ=255) aCTGTGAGTTATCGTCACTGCGTGCTGCGCCTTCAAAGGTAAAATCGCCGACTTGTTGCAGCCCGGTCa < 1:1543739/69‑1 (MQ=255) tgtgAGTTATCGTCACTGCGTGCTGCGCCTTCAAAGGTAAAATCGCCGACTTGTTGCAGCCCGGTCaga < 1:1835946/69‑1 (MQ=255) tgtgAGTTATCGTCACTGCGTGCTGCGCCTTCAAAGGTAAAATCGCCGACTTGTTGCAGCCCGGTCaga < 1:3300946/69‑1 (MQ=255) tgtgAGTTATCGTCACTGCGTGCTGCGCCTTCAAAGGTAAAATCGCCGACTTGTTGCAGCCCGGTCaga < 1:1669890/69‑1 (MQ=255) aGTTATCGTCACTGCTTGCTGCGCCTTCAAAGGTAAAATCGCCGACTTGTTGCAGCCCGGTCagata < 1:2651043/67‑1 (MQ=255) aGTTATCGTCACTGCGTGCTGCGCCTTCAAAGGTAAAATCGCCGACTTGTTGCAGCCCGGTCagataga > 1:2376560/1‑69 (MQ=255) aGTTATCGTCACTGCGTGCTGCGCCTTCAAAGGTAAAATCGCCGACTTGTTGCAGCCCGGTCagataga > 1:1108553/1‑69 (MQ=255) aGTTATCGTCACTGCGTGCTGCGCCTTCAAAGGTAAAATCGCCGACTTGTTGCAGCCCGGTCagata < 1:2777580/67‑1 (MQ=255) attATCGTCGCTGCGTCCTGCGCCTTCAAAAGTAAAATCGCCGACTTGCTGCAGCCCAGTCagatagat < 1:2314032/68‑1 (MQ=255) attATCGTCGCTGCGTCCTGCGCCTTCAAAAGTAAAATCGCCGACTTGCTGCAGCCCAGTCagatagat < 1:1743977/68‑1 (MQ=255) attATCGTCGCTGCGTCCTGCGCCTTCAAAAGTAAAATCGCCGACTTGCTGCAGCCCAGTCagatagat < 1:1826605/68‑1 (MQ=255) attATCGTCGCTGCGTCCTGCGCCTTCAAAAGTAAAATCGCCGACTTGCTGCAGCCCAGTCagatagat < 1:448117/68‑1 (MQ=255) attATCGTCGCTGCGTCCTGCGCCTTCAAAAGTAAAATCGCCGACTTGCTGCAGCCCAGTCagatagat < 1:1990297/68‑1 (MQ=255) attATCGTCGCTGCGTCCTGCGCCTTCAAAAGTAAAATCGCCGACTTGCTGCAGCCCAGTCagatagat < 1:1994254/68‑1 (MQ=255) aTCGTCACTGCGTGCTGCGCCTTTAAAGGTAAAATCGCCGACTTGTTGCAGCCCGGTCAGATAGATGcc > 1:1224645/1‑69 (MQ=255) aTCGTCACTGCGTGCTGCGCCTTCAAAGGTAAAATCGCCGACTTGTTGCAGCCCGGTCa > 1:2820658/1‑59 (MQ=255) aTCGTCACTGCGTGCTGCGCCTTCAAAGGTAAAATCGCCGACTTGTTGCAGCCCGGTCa > 1:2971345/1‑59 (MQ=255) aTCGTCACTGCGTGCTGCGCCTTCAAAGGTAAAATCGCCGACTTGTTGCAGCCCGGTCa > 1:331614/1‑59 (MQ=255) aTCGTCACTGCGTGCTGCGCCTTCAAAGGTAAAATCGCCGACTTGTTGCAGCCCGGTCa > 1:3329377/1‑59 (MQ=255) aTCGTCACTGCGTGCTGCGCCTTCAAAGGTAAAATCGCCGACTTGTTGCAGCCCGGTCa > 1:1497401/1‑59 (MQ=255) aTCGTCACTGCGTGCTGCGCCTTCAAAGGTAAAATCGCCGACTTGTTGCAGCCCGGTCa > 1:1307019/1‑59 (MQ=255) aTCGTCACTGCGTGCTGCGCCTTCAAAGGTAAAATCGCCGACTTGTTGCAGCCCGGTCAGATGGATGcc > 1:1805626/1‑69 (MQ=255) aTCGTCACTGCGTGCTGCGCCTTCAAAGGTAAAATCGCCGACTTGTTGCAGCCCGGTCAGATAGATGcc > 1:2566580/1‑69 (MQ=255) aTCGTCACTGCGTGCTGCGCCTTCAAAGGTAAAATCGCCGACTTGTTGCAGCCCGGTCAGATAGATGcc > 1:2783055/1‑69 (MQ=255) aTCGTCACTGCGTGCTGCGCCTTCAAAGGTAAAATCGCCGACTTGTTGCAGCCCGGTCAGATAGATGcc > 1:385943/1‑69 (MQ=255) aTCGTCACTGCGTGCTGCGCCTTCAAAGGTAAAATCGCCGACTTGTTGCAGCCCGGTCAGATAGATGcc > 1:2764923/1‑69 (MQ=255) aTCGTCACTGCGTGCTGCGCCTTCAAAGGTAAAATCGCCGACTTGTTGCAGCCCGGTCAGATAGATGcc > 1:447381/1‑69 (MQ=255) aTCGTCACTGCGTGCTGCGCCTTCAAAGGTAAAATCGCCGACTTGTTGCAGCCCGGTCAGATAGATGcc > 1:2651802/1‑69 (MQ=255) aTCGTCACTGCGTGCTGCGCCTTCAAAGGTAAAATCGCCGACTTGTTGCAGCCCGGTCAGATAGATGcc > 1:963159/1‑69 (MQ=255) aTCGTCACTGCGTGCTGCGCCTTCAAAGGTAAAATCGCCGACTTGTTGCAGCCCGGTCAGATAGATGcc > 1:1087534/1‑69 (MQ=255) aTCGTCACTGCGTGCTGCGCCTTCAAAGGTAAAATCGCCGACTTGTTGCAGCCCGGTCAGATAGATGcc > 1:1287081/1‑69 (MQ=255) aTCGTCACTGCGTGCTGCGCCTTCAAAGGTAAAATCGCCGACTTGTTGCAGCCCGGTCAGATAGATGcc > 1:2409963/1‑69 (MQ=255) aTCGTCACTGCGTGCTGCGCCTTCAAAGGTAAAATCGCCGACTTGTTGCAGCCCGGTCAGATAGATGcc > 1:1364270/1‑69 (MQ=255) aTCGTCACTGCGTGCTGCGCCTTCAAAGGTAAAATCGCCGACTTGTTGCAGCCCGGTCAGATAGATGcc > 1:1765728/1‑69 (MQ=255) aTCGTCACTGCGTACTGCGCCTTCAAAGGTAAAATCGCCGACTTGTTGCAGCCCGGTCAGATAGATGcc > 1:28792/1‑69 (MQ=255) gTCACTGCGTGCTGCGCCTTCAAAGGTAAAATCGCCGACTTGTTGCAGCCCGGTCAGATAGATGCCgg < 1:1088309/68‑1 (MQ=255) gTCACTGCGTGCTGCGCCTTCAAAGGTAAAATCGCCGACTTGTTGCAGCCCGGTCAGATAGATGCCGGt > 1:1008764/1‑69 (MQ=255) gTCACTGCGTGCTGCGCCTTCAAAGGTAAAATCGCCGACTTGTTGCAGCCCGGTCAGATAGATGCCGGt > 1:59919/1‑69 (MQ=255) gTCACTGCGTGCTGCGCCTTCAAAGGTAAAATCGCCGACTTGTTGCAGCCCGGTCAGATAGATGCCGGt > 1:2892193/1‑69 (MQ=255) gTCACTGCGTGCTGCGCCTTCAAAGGTAAAATCGCCGACTTGTTGCAGCCCGGTCAGATAGATGCCGGt > 1:2039455/1‑69 (MQ=255) cGTGCTGCGCCTTCAAAGGTAAAATCGCCGACTTGTTGCAGCCCGGTCAGATAGATGCCGGTATTACg > 1:671523/1‑68 (MQ=255) cGTGCTGCGCCTTCAAAGGTAAAATCGCCGACTTGTTGCAGCCCGGTCAGATAGATGCCGGTATTACg > 1:501596/1‑68 (MQ=255) tgctgcGCCTTCAAAGGTAAAATCGCCGACTTGTTGCAGCCCTGTCagataga > 1:1042312/1‑53 (MQ=255) tgctgcGCCTTCAAAGGTAAAATCGCCGACTTGTTGCAGCCCGGTCagataga > 1:2663400/1‑53 (MQ=255) tgctgcGCCTTCAAAGGTAAAATCGCCGACTTGTTGCAGCCCGGTCagatag > 1:2695397/1‑52 (MQ=255) tgctgcGCCTTCAAAGGTAAAATCGCCGACTTGTTGCAGCCCGGTCaga > 1:1720942/1‑49 (MQ=255) cgcCTTCAAAGGTAAAATCGCCGACTTGTTGCAGCCCGGTCAGATAGATGCCGGTAt < 1:2522180/57‑1 (MQ=255) cTTCAAAGGTAAAATCGCCGACTTGTTGCAGCCCGGTCAGATAGATGCCGGTATTACGTTGATCATatc < 1:2550816/69‑1 (MQ=255) cTTCAAAGGTAAAATCGCCGACTTGTTGCAGCCCGGTCAGATAGATGCCGGTATTACGTTGATCATatc < 1:598140/69‑1 (MQ=255) ttCAAAGGTAAAATCGCCGACTTGTTGCAGCCCGGTCAGATAGATGCCGGTATTACGTTGATCATatc < 1:2905848/68‑1 (MQ=255) tCAAAGGTAAAATCGCCGACTTGTTGCAGCCCGGTCAGATAGATGCCGGTATTACGTTGATCATatc < 1:2676456/67‑1 (MQ=255) tAAAATCGCCGACTTGTTGCAGCCCGGTCAGATAGATGCCGGTATTACGTTGATCATATCCATCCTCaa < 1:433451/69‑1 (MQ=255) tAAAATCGCCGACTTGTTGCAGCCCGGTCAGATAGATGCCGGTATTACGTTGATCATATCCATCCTCaa < 1:54765/69‑1 (MQ=255) tAAAATCGCCGACTTGTTGCAGCCCGGTCAGATAGATGCCGGTATTACGTTGATCATATCCATCCTCaa < 1:1226990/69‑1 (MQ=255) tAAAATCGCCGACTTGTTGCAGCCCGGTCAGATAGATGCCGGTATTACGTTGATCATATCCATCCTCaa < 1:2253620/69‑1 (MQ=255) tAAAATCGCCGACTTGTTGCAGCCCGGTCAGATAGATGCCGGTATTACGTTGATCATATCCATCCTCaa < 1:223648/69‑1 (MQ=255) tAAAATCGCCGACTTGTTGCAGCCCGGTCAGATAGATGCCGGTATTACGTTGATCATATCCATCCTCaa < 1:306603/69‑1 (MQ=255) aaaaTCGCCGACTTGTTGCAGCCCGGTCAGATAGATGCCGGTATTACGTTGATCATATCCATCCTCaa > 1:2421316/1‑68 (MQ=255) aaaaTCGCCGACTTGTTGCAGCCCGGTCAGATAGATGCCGGTATTACGTTGATCATATCCATCCTCaa < 1:491933/68‑1 (MQ=255) aaaaTCGCCGACTTGTTGCAGCCCGGTCAGATAGATGCCGGTATTACGTTGATCATATCCATCCTCaa > 1:1824702/1‑68 (MQ=255) gCCGACTTGTTGCAGCCCGGTCAGATAGATGCCGGTATTACGTTGATCATATCCATCCTCAACATAAcc < 1:2498274/69‑1 (MQ=255) gCCGACTTGTTGCAGCCCGGTCAGATAGATGCCGGTATTACGTTGATCATATCCATCCTCAACATAAcc < 1:3020572/69‑1 (MQ=255) ccGACTTGTTGCAGCCCGGTCAGATAGATGCCCGTATTACGTTGATCATATCCATCCTCAACATAAcc < 1:682958/68‑1 (MQ=255) cGACTTGTTGCAGCCCGGTCAGATAGATGCCGGTATTACGTTGATCATATCCATCCTCAACATAACCTg < 1:3435482/69‑1 (MQ=255) gttgCAGCCCGGTCAGATAGATGCCGGTATTACGTTGATCATATCCATCCTCAACATAACCTg > 1:1315447/1‑63 (MQ=255) gttgCAGCCCGGTCAGATAGATGCCGGTATTACGTTGATCATATCCATCCTCAACATAACCTg > 1:2170319/1‑63 (MQ=255) gttgCAGCCCGGTCAGATAGATGCCGGTATTACGTTGATCATATCCATCCTCAACATAACCTg > 1:1163961/1‑63 (MQ=255) gttgCAGCCCGGTCAGATAGATGCCGGTATTACGTTGATCATATCCATCCTCAACATAACCTg > 1:1103125/1‑63 (MQ=255) gttgCAGCCCGGTCAGATAGATGCCGGTATTACGTTGATCATATCCATCCTCAACATAACCTg > 1:3247982/1‑63 (MQ=255) | ACGACCAAACTGTGAGTTATCGTCACTGCGTGCTGCGCCTTCAAAGGTAAAATCGCCGACTTGTTGCAGCCCGGTCAGATAGATGCCGGTATTACGTTGATCATATCCATCCTCAACATAACCTG > minE/2277588‑2277712 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |