| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,278,398 | G→A | 28.5% | S117S (AGC→AGT) | btuB ← | vitamin B12/cobalamin outer membrane transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,278,398 | 0 | G | A | 28.5% | 85.1 / 32.0 | 82 | S117S (AGC→AGT) | btuB | vitamin B12/cobalamin outer membrane transporter |
| Reads supporting (aligned to +/- strand): ref base G (42/15); new base A (17/6); total (61/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACCATAAACAGCGGAGCGCGGCCCACGGATATATTCAACACGCTGGACAAGCGCAATAGGGAACTGGCTAAGGTCGGCAGAACCACTCACCCCCGCCAGATTCAGGCGTACGCCATCAATTAACACCAACA > minE/2278332‑2278462 | aCCATAAACAGCGGAGCGCGGCCCACGGATATATTCAACACGCTGGACAAGCGCAATAGGGAACTgg > 1:1709006/1‑67 (MQ=255)aCCATAAACAGCGGAGCGCGGCCCACGGATATATTCAACACGCTGGACAAGCGCAATAGGGAACTgg > 1:540276/1‑67 (MQ=255)aCCATAAACAGCGGAGCGCGGCCCACGGATATATTCAACACGCTGGACAAGCGCAATAGGGAACTgg > 1:1720678/1‑67 (MQ=255)aCCATAAACAGCGGAGCGCGGCCCACGGATATATTCAACACGCTGGACAAGCGCAATAGGGAACTgg > 1:2997431/1‑67 (MQ=255)aCCATAAACAGCGGAGCGCGGCCCACGGATATATTCAACACGCTGGACAAGCGCAATAGGGAACTgg > 1:2879070/1‑67 (MQ=255)aCCATAAACAGCGGAGCGCGGCCCACGGATATATTCAACACGCTGGACAAGCGCAATAGGGAACTgg > 1:3224479/1‑67 (MQ=255)aCCATAAACAGCGGAGCGCGGCCCACGGATATATTCAACACGCTGGACAAGCGCAATAGGGAACTgg > 1:2985009/1‑67 (MQ=255)aCCATAAACAGCGGAGCGCGGCCCACGGATATATTCAACACGCTGGACAAGCGCAATAGGGAACTgg > 1:1882917/1‑67 (MQ=255) aTAAACAGCGGAGCGCGGCCCACGGATATATTCAACACGCTGGACAAGCGCAATAGGTAACTGGCTAAg > 1:512236/1‑69 (MQ=255) aTAAACAGCGGAGCGCGGCCCACGGATATATTCAACACGCTGGACAAGCGCAATAGGGAACTGGCTaa > 1:3429119/1‑68 (MQ=255) aTAAACAGCGGAGCGCGGCCCACGGATATATTCAACACGCTGGACAAGCGCAATAGGGAACTGGCTAAg < 1:1227456/69‑1 (MQ=255) aTAAACAGCGGAGCGCGGCCCACGGATATATTCAACACGCTGGACAAGCGCAATAGGGAACTGGCTAAg > 1:2893434/1‑69 (MQ=255) aTAAACAGCGGAGCGCGGCCCACGGATATATTCAACACGCTGGACAAGCGCAATAGGGAACTGGCTAAg > 1:2796826/1‑69 (MQ=255) aTAAACAGCGGAGCGCGGCCCACGGATATATTCAACACGCTGGACAAGCGCAATAGGGAACTGGCTAAg > 1:149357/1‑69 (MQ=255) aTAAACAGCGGAGCGCGGCCCACGGATATATTCAACACGCTGGACAAGCGCAATAGGGAACTGGCTAAg > 1:3048992/1‑69 (MQ=255) aTAAACAGCGGAGCGCGGCCCACGGATATATTCAACACGCTGGACAAGCGCAATAGGGAACTGGCTAAg > 1:2189815/1‑69 (MQ=255) aTAAACAGCGGAGCGCGGCCCACGGATATATTCAACACGCTGGACAAGCGCAATAGGGAACTGGCTAAg > 1:742327/1‑69 (MQ=255) aTAAACAGCGGAGCGCGGCCCACGGATATATTCAACACGCTGGACAAGCGCAATAGGGAACTGGCTAAg > 1:1720340/1‑69 (MQ=255) aTAAACAGCGGAGCGCGGCCCACGGATATATTCAACACGCTGGACAACCGCAATAGGGAACTGGCTAAg > 1:1820072/1‑69 (MQ=255) cAGCGGAGCGCGGCCCACGGATATATTCAACACGCTGGACAAGCGCAATAGGGAACTGGCTAAGGTCgg < 1:1899446/69‑1 (MQ=255) cAGCGGAGCGCGGCCCACGGATATATTCAACACGCTGGACAAGCGCAATAGGGAACTGGCTAAGGTCgg < 1:3427642/69‑1 (MQ=255) cAGCGGAGCGCGGCCCACGGATATATTCAACACGCTGGACAAGCGCAATAGGGAACTGGCTAAGGTCgg < 1:1413371/69‑1 (MQ=255) cGGAGCGCGGCCCACGGATATATTCAACACGCTGGACAAGCGCAATAGGGAACTGGCTAAGGTCGGCAg < 1:1596690/69‑1 (MQ=255) cGGAGCGCGGCCCACGGATATATTCAACACGCTGGACAAGCGCAATAGGGAACTGGCTAAGGTCGGCAg < 1:749399/69‑1 (MQ=255) cgcgGCCCACGGATATATTCAACACGCTGGACAAGCGCAATAGGGAACTGACTAAGATCAGCAGAACCa < 1:561699/69‑1 (MQ=255) cgcgGCCCACGGATATATTCAACACGCTGGACAAGCGCAATAGGGAACTGACTAAGATCAGCAGAACCa < 1:1161841/69‑1 (MQ=255) cgcgGCCCACGGATATATTCAACACGCTGGACAAGCGCAATAGGGAACTGACTAAGATCAGCAGAACCa < 1:2923044/69‑1 (MQ=255) cgcgGCCCACGGATATATTCAACACGCTGGACAAGCGCAATAGGGAACTGACTAAGATCAGCAGAACCa < 1:1904169/69‑1 (MQ=255) cgcgGCCCACGGATATATTCAACACGCTGGACAAGCGCAATAGGGAACTGACTAAGATCAGCAGAACCa < 1:1268772/69‑1 (MQ=255) gcgGCCCACGGATATATTCAACACGCTGGACAAGCGCAATAGGGAACTGACTAAGATCAGCAGAACCa < 1:1611373/68‑1 (MQ=255) tatatTCAACACGCTGGACAAGCGCAATAGGGAACTGGCTATGGTCgg < 1:557064/48‑1 (MQ=255) acacGCTGGACAAGCGCAATAGGGAACTGGCTAAGGTCGGCAGAACCa < 1:1129110/48‑1 (MQ=255) acacGCTGGACAAGCGCAATAGGGAACTGGCTAAGGTCGGCAGAACCa < 1:2612304/48‑1 (MQ=255) acGCTGGACAAGCGCAATAGGGAACTGGCTAAGGTCGGCAGAACCACTCACCCCCGCCAGATTCAGGCg < 1:1681071/69‑1 (MQ=255) acGCTGGACAAGCGCAATAGGGAACTGGCTAAGGTCGGCAGAACCACTCACCCCCGCCAGATTCAGGCg < 1:89359/69‑1 (MQ=255) acGCTGGACAAGCGCAATAGGGAACTGGCTAAGGTCGGCAGAACCACTCACCCCCGCCAGATTCAGGCg < 1:709851/69‑1 (MQ=255) cGCTGGACAAGCGCAATAGGGAACTGGCTAAGGTCGGCAGAACCACTCACCCCCGCCAGATTCAGGCg < 1:1756036/68‑1 (MQ=255) cTGGACAAGCGCAATAGGGAACTGACTAAGATCAGCAGAACCACTCACCCCCGCCAAATTCAGGCGGAc > 1:1697370/1‑69 (MQ=255) cTGGACAAGCGCAATAGGGAACTGACTAAGATCAGCAGAACCACTCACCCCCGCCAAATTCAGGCGGAc > 1:2340097/1‑69 (MQ=255) cTGGACAAGCGCAATAGGGAACTGACTAAGATCAGCAGAACCACTCACCCCCGCCAAATTCAGGCGGAc > 1:267662/1‑69 (MQ=255) cTGGACAAGCGCAATAGGGAACTGACTAAGATCAGCAGAACCACTCACCCCCGCCAAATTCAGGCGGAc > 1:2450457/1‑69 (MQ=255) cTGGACAAGCGCAATAGGGAACTGACTAAGATCAGCAGAACCACTCACCCCCGCCAAATTCAGGCGGAc > 1:3351368/1‑69 (MQ=255) cTGGACAAGCGCAATAGGGAACTGACTAAGATCAGCAGAACCACTCACCCCCGCCAAATTCAGGCGGAc > 1:3263562/1‑69 (MQ=255) cTGGACAAGCGCAATAGGGAACTGACTAAGATCAGCAGAACCACTCACCCCCGCCAAATTCAGGCGGAc > 1:1905095/1‑69 (MQ=255) cTGGACAAGCGCAATAGGGAACTGACTAAGATCAGCAGAACCACTCACCCCCGCCAAATTCAGGCGGAc > 1:575522/1‑69 (MQ=255) cTGGACAAGCGCAATAGGGAACTGACTAAGATCAGCAGAACCACTCACCCCCGCCAAATTCAGGCGGAc > 1:3236452/1‑69 (MQ=255) cgcAATAGGGAACTGTCTAAGGTCGGCAGAACCACTCACCCCCGCCAGATTCAGGCGTACGCCATCaa > 1:1126375/1‑68 (MQ=255) cgcAATAGGGAACTGGCTAAGGTCGGCAGAACCACTCACCCCCGCCAGATTCAGGCGTACGCCATCaa > 1:2746433/1‑68 (MQ=255) cgcAATAGGGAACTGGCTAAGGTCGGCAGAACCACTCACCCCCGCCAGATTCAGGCGTACGCCATCaa > 1:962531/1‑68 (MQ=255) cgcAATAGGGAACTGGCTAAGGTCGGCAGAACCACTCACCCCCGCCAGATTCAGGCGTACGCCATCaa > 1:2484857/1‑68 (MQ=255) cgcAATAGGGAACTGGCTAAGGTCGGCAGAACCACTCACCCCCGCCAGATTCAGGCGTACGCCATCaa > 1:1087193/1‑68 (MQ=255) cgcAATAGGGAACTGCCTAAGATCAGCAGAACCACTCACCCCCGCCAAATTCAGGCGGACGCCAt > 1:3236659/1‑65 (MQ=255) cgcAATAGGGAACTGACTAAGATCAGCAGAACCACt > 1:196886/1‑36 (MQ=25) cgcAATAGGGAACTGACTAAGATCAGCAGAACCACTCACCCCCGCCAAATTCAGGCGGACGCCATCaa > 1:41625/1‑68 (MQ=255) cgcAATAGGGAACTGACTAAGATCAGCAGAACCACTCACCCCCGCCAAATTCAGGCGGACGCCATCaa > 1:1732736/1‑68 (MQ=255) cgcAATAGGGAACTGACTAAGATCAGCAGAACCACTCACCCCCGCCAAATTCAGGCGGACGCCATCaa > 1:1778857/1‑68 (MQ=255) cgcAATAGGGAACTGACTAAGATCAGCAGAACCACTCACCCCCGCCAAATTCAGGCGGACGCCATCaa > 1:1878934/1‑68 (MQ=255) cgcAATAGGGAACTGACTAAGATCAGCAGAACCACTCACCCCCGCCAAATTCAGGCGGACGCCATCaa > 1:1908508/1‑68 (MQ=255) cgcAATAGGGAACTGACTAAGATCAGCAGAACCACTCACCCCCGCCAAATTCAGGCGGACGCCATCaa > 1:2387653/1‑68 (MQ=255) cgcAATAGGGAACTGACTAAGATCAGCAGAACCACTCACCCCCGCCAAATTCAGGCGGACGCCATCaa > 1:2509478/1‑68 (MQ=255) aaTAGGGAACTGGCTAAGGTCGGCAGAACCACTCACCCCCGCCAGATTCAGGCGTACGCCATCAATTaa < 1:2267532/69‑1 (MQ=255) gtgAACTGGCTAAGGTCGGCAGAACCACTCACCCCCGCCAGATTCAGGCGTACGCCATCAATTAACAcc > 1:1396892/3‑69 (MQ=255) gggAACTGGCTAAGGTCGGCAGAACCACTCACCCCCGCCAGATTCAGGCGTACGc > 1:2449884/1‑55 (MQ=255) gggAACTGGCTAAGGTCGGCAGAACCACTCACCCCCGCCAGATTCAGGCGTACGCCATCaaat > 1:1933855/1‑61 (MQ=255) gggAACTGGCTAAGGTCGGCAGAACCACTCACCCCCGCCAGATTCAGGCGTACGCCATCAAtt > 1:1196357/1‑63 (MQ=255) gggAACTGGCTAAGGTCGGCAGAACCACTCACCCCCGCCAGATTCAGGCGTACGCCATCAAtt > 1:765186/1‑63 (MQ=255) gggAACTGGCTAAGGTCGGCAGAACCACTCACCCCCGCCAGATTCAGGCGTACGCCATCAAtt > 1:2439129/1‑63 (MQ=255) gggAACTGGCTAAGGTCGGCAGAACCACTCACCCCCGCCAGATTCAGGCGTACGCCATCAAtt > 1:3034357/1‑63 (MQ=255) gggAACTGGCTAAGGTCGGCAGAACCACTCACCCCCGCCAGATTCAGGCGTACGCCATCAAtt > 1:1874111/1‑63 (MQ=255) gggAACTGGCTAAGGTCGGCAGAACCACTCACCCCCGCCAGATTCAGGCGTACGCCATCAAtt > 1:1941097/1‑63 (MQ=255) gggAACTGGCTAAGGTCGGCAGAACCACTCACCCCCGCCAGATTCAGGCGTACGCCATCAAtt > 1:2690333/1‑63 (MQ=255) gggAACTGGCTAAGGTCGGCAGAACCACTCACCCCCGCCAGATTCAGGCGTACGCCATCAATTAacac > 1:3087180/1‑68 (MQ=255) gggAACTGGCTAAGGTCGGCAGAACCACTCACCCCCGCCAGATTCAGGCGTACGCCATCAATTAaca > 1:1147402/1‑67 (MQ=255) gggAACTGGCTAAGGTCGGCAGAACCACTCACCCCCGCCAGATTCAGGCGTACGCCATCAATTAACAcc > 1:660882/1‑69 (MQ=255) gggAACTGGCTAAGGTCGGCAGAACCACTCACCCCCGCCAGATTCAGGCGTACGCCATCAATTAACAcc > 1:2308404/1‑69 (MQ=255) gggAACTGGCTAAGGTCGGCAGAACCACTCACCCCCGCCAGATTCAGGCGTACGCCATCAATTAACAcc > 1:312474/1‑69 (MQ=255) gggAACTGGCTAAGGTCGGCAGAACCACTCACCCCCGCCAGATTCAGGCGTACGCCATCAATTAACAcc > 1:3072583/1‑69 (MQ=255) gggAACTGGCTAAGGTCGGCAGAACCACTCACCCCCGCCAGATTCAGGCGTACGCCATCAATTAACAcc > 1:2625955/1‑69 (MQ=255) gggAACTGGCTAAGGTCGGCAGAACCACTCACCCCCGCCAGATTCAGGCGTACGCCATCAATTAACAcc > 1:2281550/1‑69 (MQ=255) gggAACTGGCTAAGGTCGGCAGAACCACTCACCCCCGCCAGATTCAGGCGTACGCCATCAATTAACAcc > 1:227042/1‑69 (MQ=255) gggAACTGGCTAAGGTCGGCAGAACCACTCACCCCCGCCAGATTCAGGCGTACGCCATCAATTAACAcc > 1:2176952/1‑69 (MQ=255) gggAACTGGCTAAGGTCGGCAGAACCACTCACCCCCGCCAGATTCAGGCGTACGCCATCAATTAACAcc > 1:1899359/1‑69 (MQ=255) gggAACTGGCTAAGGTCGGCAGAACCACTCACCCCCGCCAGATTCAGGCGTACGCCATCAATTAACAcc > 1:1692203/1‑69 (MQ=255) gggAACTGGCTAAGGTCGGCAGAACCACTCACCCCCGCCAGATTCAGGCGTACGCCATCAATTAACAcc > 1:1655858/1‑69 (MQ=255) gggAACTGGCTAAGGTCGGCAGAACCACTCACCCCCGCCAGATTCAGGCGTACGCCATCAATTAACAcc > 1:1475264/1‑69 (MQ=255) gggAACTGGCTAAGGTCGGCAGAACCACTCACCCCCGCCAGATTCAGGCGTACGCCATCAATTAACAcc > 1:145630/1‑69 (MQ=255) gggAACTGGCTAAGGTCGGCAGAACCACTCACCCCCGCCAGATTCAGGCGTACGCCATCAATTAACAcc > 1:1427925/1‑69 (MQ=255) gggAACTGGCTAAGGTCGGCAGAACCACTCACCCCCGCCAGATTCAGGCGTACGCCATCAATTAACAcc > 1:1117434/1‑69 (MQ=255) gggAACTGGCTAAGGTCGGCAGAACCACTCACCACCGCCAGATTCAGGCGTACGCCATCAATTAACAcc > 1:727746/1‑69 (MQ=255) aCTGGCTAAGGTCGGCAGAACCACTCACCCCCGCCAGATTCAGGCGTACGCCATCAATTAACACCAaca < 1:1625952/69‑1 (MQ=255) | ACCATAAACAGCGGAGCGCGGCCCACGGATATATTCAACACGCTGGACAAGCGCAATAGGGAACTGGCTAAGGTCGGCAGAACCACTCACCCCCGCCAGATTCAGGCGTACGCCATCAATTAACACCAACA > minE/2278332‑2278462 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |