| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,279,636 | G→A | 29.2% | V174I (GTC→ATC) | trmA → | tRNA (uracil‑5‑)‑methyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,279,636 | 0 | G | A | 29.2% | 94.4 / 39.4 | 92 | V174I (GTC→ATC) | trmA | tRNA (uracil‑5‑)‑methyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (31/34); new base A (17/10); total (48/44) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.52e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.40e-01 | |||||||||||
CTGATTGGTCGGGCAACGAAAACCAAAATCGAGCTGGATCAGGATTACATCGATGAACGTCTGCCGGTCGCAGGGAAAGAGATGATCTACCGTCAGGTAGAAAACAGCTTTACCCAGCCGAACGCGGCGATGAA > minE/2279570‑2279703 | cTGATTGGTCGGGCAACGAAAACCAAAATCGAGCTGGATCAGGATTACATCGATGAACGTCTGCCGGt > 1:3164953/1‑68 (MQ=255) ggTCGGGCAACGAAAACCAAAATCGAGCTGGATCAGGATTACATCGATGAACGTCTGCCGGTCGCAggg < 1:3119116/69‑1 (MQ=255) ggTCGGGCAACGAAAACCAAAATCGAGCTGGATCAGGATTACATCGATGAACGTCTGCCGGTCGCAggg < 1:479626/69‑1 (MQ=255) ggTCGGGCAACGAAAACCAAAATCGAGCTGGATCAGGATTACATCGATGAACGTCTGCCGGTCGCAggg < 1:1196361/69‑1 (MQ=255) tCGGGCAACGAAAACCAAAATCGAGCTGGATCAGGATTACATCGATGAACGTCTGCCGGTCGCAGGGa < 1:1711634/68‑1 (MQ=255) tCGGGCAACGAAAACCAAAATCGAGCTGGATCAGGATTACATCGATGAACGTCTGCCGGTCGCAGGGa < 1:3263605/68‑1 (MQ=255) tCGGGCAACGAAAACCAAAATCGAGCTGGATCAGGATTACATCGATGAACGTCTGCCGGTCGCAGGGa < 1:2025096/68‑1 (MQ=255) tCGGGCAACGAAAACCAAAATCGAGCTGGATCAGGATTACATCGATGAACGTCTGCCGGTCGCAGGGa < 1:2767015/68‑1 (MQ=255) tCGGGCAACGAAAACCAAAATCGAGCTGGATCAGGATTACATCGATGAACGTCTGCCGGTCGCAGGGa < 1:1164268/68‑1 (MQ=255) cAACGAAAACCAAAATCGAG‑‑GGATCAGGATTACATCGATGAACGTCTGCCGGCCGCAGGGAAAGAgatg < 1:2707685/69‑1 (MQ=255) tcGAAAACCAAAATCGAGCTGGATCAGTATTACATCGATGAACGTCTGCCGGTCGCAGGGAAAGAgatg < 1:2276098/68‑1 (MQ=255) aCGAAAACCAAAATCGAGCTGGATCAGGATTACATCGATGAACGTCTGCCGGTCGCAGGGAAAGAgatg < 1:740521/69‑1 (MQ=255) aCGAAAACCAAAATCGAGCTGGATCAGGATTACATCGATGAACGTCTGCCGGTCGCAGGGAAAGAgatg < 1:2843835/69‑1 (MQ=255) aCGAAAACCAAAATCGAGCTGGATCAGGATTACATCGATGAACGTCTGCCGGTCGCAGGGAAAGAgatg < 1:2296320/69‑1 (MQ=255) aCGAAAACCAAAATCGAGCTGGATCAGGATTACATCGATGAACGTCTGCCGGTCGCAGGGAAAGAgatg < 1:3339138/69‑1 (MQ=255) aCGAAAACCAAAATCGAGCTGGATCAGGATTACATCGATGAACGTCTGCCGGTCGCAGGGAAAGAgatg < 1:1587833/69‑1 (MQ=255) aCGAAAACCAAAATCGAGCTGGATCAGGATTACATCGATGAACGTCTGCCGGTCGCAGGGAAAGAgatg < 1:2349738/69‑1 (MQ=255) aCGAAAACCAAAATCGAGCTGGATCAGGATTACATCGATGAACGTCTGCCGGTCGCAGGGAAAGAgatg < 1:3245187/69‑1 (MQ=255) gAAAACCAAAATCGAGCTGGATCAGGATTACATCGATGAACGTCTGCCGGTCGCAGGGAAAGAgatga > 1:2274507/1‑68 (MQ=255) gAAAACCAAAATCGAGCTGGATCAGGATTACATCGATGAACGTCTGCCGGTCGCAGGGAAAGAgatga > 1:2998198/1‑68 (MQ=255) gAAAACCAAAATCGAGCTGGATCAGGATTACATCGATGAACGTCTGCCGGTCGCAGGGAAAGAgatga > 1:2103285/1‑68 (MQ=255) gAAAACCAAAATCGAGCTGGATCAGGATTACATCGATGAACGTCTGCCGGTCGCAGGGAAAGAgatga > 1:3157646/1‑68 (MQ=255) gAAAACCAAAATCGAGCTGGATCAGGATTACATCGATGAACGTCTGCCGGTCGCAGGGAAAGAgatga > 1:2507901/1‑68 (MQ=255) gAAAACCAAAATCGAGCTGGATCAGGATTACATCGATGAACGTCTGCCGGTCGCAGGGAAAGAgatga > 1:801299/1‑68 (MQ=255) gAAAACCAAAATCGAGCTGGATCAGGATTACATCGATGAACGTCTGCCGGTCGCAGGGAAAGAgatga > 1:1445521/1‑68 (MQ=255) gAAAACCAAAATCGAGCTGGATCAGGATTACATCGATGAACGTCTGCCGGTCGCAGGGAAAGAgatga > 1:1213381/1‑68 (MQ=255) cGAGCTGGATCAGGATTACATCGATGAACGTCTGCCGGTCGCAGGGAAAGAgatg < 1:1750704/55‑1 (MQ=255) aGCTGGATCAGGATTACATCGATGAACGGCTGCCGGTCGCAGGGa < 1:3295365/45‑1 (MQ=255) cAGGATTACATCGATGAACGTCTGCCGGTCGCAGGGAAAGAGATGATCTACCGTCAGGTAGAAAACAGc > 1:1332376/1‑69 (MQ=255) cAGGATTACATCGATGAACGTCTGCCGGTCGCAGGGAAAGAGATGATCTACCGTCAGGTAGAAAACAGc > 1:1150674/1‑69 (MQ=255) cAGGATTACATCGATGAACGTCTGCCGGTCGCAGGGAAAGAGATGATCTACCGTCAGGTAGAAAACAGc > 1:1166928/1‑69 (MQ=255) cAGGATTACATCGATGAACGTCTGCCGGTCGCAGGGAAAGAGATGATCTACCGTCAGGTAGAAAACAGc > 1:717285/1‑69 (MQ=255) cAGGATTACATCGATGAACGTCTGCCGGTCGCAGGGAAAGAGATGATCTACCGTCAGGTAGAAAACAGc > 1:2223441/1‑69 (MQ=255) cAGGATTACATCGATGAACGTCTGCCGGTCGCAGGGAAAGAGATGATCTACCGTCAGGTAGAAAACAGc > 1:284245/1‑69 (MQ=255) cAGGATTACATCGATGAACGTCTGCCGGTCGCAGGGAAAGAGATGATCTACCGTCAGGTAGAAAACAGc > 1:1690140/1‑69 (MQ=255) cAGGATTACATCGATGAACGTCTGCCGGTCGCAGGGAAAGAGATGATCTACCGTCAGGTAGAAAACAGc > 1:3051259/1‑69 (MQ=255) cAGGATTACATCGATGAACGTCTGCCGGTCGCAGGGAAAGAGATGATCTACCGTCAGGTAGAAAACAGc > 1:392006/1‑69 (MQ=255) cAGGATTACATCGATGAACGTCTGCCGGTCGCAGGGAAAGAGATGATCTACCGTCAGGTAGAAAACAGc > 1:3072781/1‑69 (MQ=255) cAGGATTACATCGATGAACGTCTGCCGGTCGCAGGGAAAGAGATGATCTACCGTCAGGTAGAAAACAGc > 1:3122466/1‑69 (MQ=255) cAGGATTACATCGATGAACGTCTGCCGGTCGCAGGGAAAGAGATGATCTACCGTCAGGTAGAAAACAGc > 1:1809221/1‑69 (MQ=255) gATTACATCGATGAACGTCTGCCGGTCGCAGGGAAAGAGATGATCTACCGTCAGGTAGaa < 1:547130/60‑1 (MQ=255) aCATCGATGAACGTCTGCCGGTCGCAGGGAAAGAGATGATCTACCGTCAGGTAGAAAACAGCTTTAccc > 1:3046368/1‑69 (MQ=255) aCATCGATGAACGTCTGCCGGTCGCAGGGAAAGAGATGATCTACCGTCAGGTAGAAAACAGCTTTAccc > 1:3315001/1‑69 (MQ=255) aCATCGATGAACGTCTGCCGGTCGCAGGGAAAGAGATGATCTACCGTCAGGTAGAAAACAGCTTTAccc > 1:826839/1‑69 (MQ=255) aCATCGATGAACGTCTGCCGGTCGCAGGGAAAGAGATGATCTACCGTCAGGTAGAAAACAGCTTTAccc > 1:110680/1‑69 (MQ=255) aCATCGATGAACGTCTGCCGGTCGCAGGGAAAGAGATGATCTACCGTCAGGTAGAAAACAGCTTTAccc > 1:1810688/1‑69 (MQ=255) aCATCGATGAACGTCTGCCGGTCGCAGGGAAAGAGATGATCTACCGTCAGGTAGAAAACAGCTTTAccc > 1:119000/1‑69 (MQ=255) aCATCGATGAACGTCTGCCGGTCGCAGGGAAAGAGATGATCTACCGTCAGGTAGAAAACAGCTTTAccc > 1:2104411/1‑69 (MQ=255) aCATCGATGAACGTCTGCCGGTCGCAGGGAAAGAGATGATCTACCGTCAGGTAGAAAACAGCTTTAccc > 1:2173223/1‑69 (MQ=255) aCATCGATGAACGTCTGCCGGTCGCAGGGAAAGAGATGATCTACCGTCAGGTAGAAAACAGCTTTAcc > 1:1104047/1‑68 (MQ=255) gATGAACGTCTGCCGGTCGCAGGGAAAGAGATGATCTACCGTCAGGTAGAAAACAGCTTTACCCAGCCg < 1:2110621/69‑1 (MQ=255) cgAACGTCTGCTGATCGCGGGGAAAGAGATGATCTACCGTCAGGTAGAAAACAGCTTTACCCAACCg < 1:1343934/66‑1 (MQ=255) cgAACGTCTGCCGATCGCGGGGAAAGATATGATCTACCGTCAGGTAGAAAACAGCTTTACCCAACCg < 1:759102/66‑1 (MQ=255) cgAACGTCTGCCGATCGCGGGGAAAGATATGATCTACCGTCAGGTAGAAAACAGCTTTACCCAACCg < 1:3283211/66‑1 (MQ=255) cgAACGTCTGCCGATCGCGGGGAAAGAGATGATCTACCGTCAGGTAGAAAACAGCTTTACCCAACCg < 1:1209287/66‑1 (MQ=255) cgAACGTCTGCCGATCGCGGGGAAAGAGATGATCTACCGTCAGGTAGAAAACAGCTTTACCCAACCg < 1:50498/66‑1 (MQ=255) cgAACGTCTGCCGATCGCGGGGAAAGAGATGATCTACCGTCAGGTAGAAAACAGCTTTACCCAACCg < 1:2173323/66‑1 (MQ=255) cgAACGTCTGCCGATCGCGGGGAAAGAGATGATCTACCGTCAGGTAGAAAACAGCTTTACCCAACCg < 1:2460577/66‑1 (MQ=255) cgAACGTCTGCCGATCGCGGGGAAAGAGATGATCTACCGTCAGGTAGAAAACAGCTTTACCCAACCg < 1:292582/66‑1 (MQ=255) cgAACGTCTGCCGATCGCGGGGAAAGAGATGATCTACCGTCAGGTAGAAAACAGCTTTACCCAACCg < 1:3410884/66‑1 (MQ=255) cgAACGTCTGCCGATCGCGGGGAAAGAGATGATCTACCGTCAGGTAGAAAACAGCTTTACCCAACCg < 1:1658936/66‑1 (MQ=255) tCTGCCGGTCGCAGGGAAAGAGATGATCTACCGTCAGGTAGAAAACAGCTTTACCCAGCCGAACgcggc < 1:1585579/69‑1 (MQ=255) tCTGCCGGTCGCAGGGAAAGAGATGATCTACCGTCAGGTAGAAAACAGCTTTACCCAGCCGAACgcggc < 1:1744560/69‑1 (MQ=255) tCTGCCGGTCGCAGGGAAAGAGATGATCTACCGTCAGGTAGAAAACAGCTTTACCCAGCCGAACgcggc < 1:3160311/69‑1 (MQ=255) tCTGCCGGTCGCAGGGAAAGAGATGATCTACCGTCAGGTAGAAAACAGCTTTACCCAGCCGAACgcggc < 1:3274886/69‑1 (MQ=255) tCTGCCGGTCGCAGGGAAAGAGATGATCTACCGTCAGGTAGAAAACAGCTTTACCCAGCCGAACgcggc < 1:2066573/69‑1 (MQ=255) tCTGCCGGTCGCAGGGAAAGAGATGATCTACCGTCAGGTAGAAAACAGCTTTACCCAGCCGAACgcggc < 1:2680264/69‑1 (MQ=255) tCTGCCGGTCGCAGGGAAAGAGATGATCTACCGTCAGGTAGAAAACAGCTTTACCCAGCCGAACgcggc < 1:2725067/69‑1 (MQ=255) tCTGCCGGTCGCAGGGAAAGAGATGATCTACCGTCAGGTAGAAAACAGCTTTACCCAGCCGAACgcggc < 1:2131855/69‑1 (MQ=255) tCTGCCGGTCGCAGGGAAAGAGATGATCTACCGTCAGGTAGAAAACAGCTTTACCCAGCCGAACgcggc < 1:1951421/69‑1 (MQ=255) gtGCCGGTCGCAGGGAAAGAGATGATCTACCGTCAGGTAGAAAACAGCTTTACCCAGCCGAACgcggc < 1:1252140/67‑1 (MQ=255) cTGCCGGTCGCAGGGAAAGAGATGATCTACCGTCAGGTAGAAATCAGCTTTACCCAGCCGAACgcggc < 1:3410202/68‑1 (MQ=255) cTGCCGGTCGCAGGGAAAGAGATGATCTACCGTCAGGTAGAAAACAGCTTTACCCAGCCGAACgcggc < 1:728304/68‑1 (MQ=255) cTGCCGGTCGCAGGGAAAGAGATGATCTACAGTCAGGTAGAAAACAGCTTTACCCAGCCGAACgcggc < 1:2077560/68‑1 (MQ=255) gCCGATCGCGGGGAAAGAGATGATCTACCGTCAGGTAGAAAACAGCttt > 1:355534/1‑49 (MQ=255) gCCGATCGCGGGGAAAGAGATGATCTACCGTCAGGTAGAAAACAGCttt > 1:2663768/1‑49 (MQ=255) gCCGATCGCGGGGAAAGAGATGATCTACCGTCAGGTAGAAAACAGCttt > 1:2216504/1‑49 (MQ=255) gCCGATCGCGGGGAAAGAGATGATCTACCGTCAGGTAGAAAACAGCttt > 1:481833/1‑49 (MQ=255) gCCGATCGCGGGGAAAGAGATGATCTACCGTCAGGTAGAAAACAGCttt > 1:417863/1‑49 (MQ=255) gCCGATCGCGGGGAAAGAGATGATCTACCGTCAGGTAGAAAACAGCttt > 1:35576/1‑49 (MQ=255) gCCGATCGCGGGGAAAGAGATGATCTACCGTCAGGTAGAAAACAGCttt > 1:347400/1‑49 (MQ=255) gCCGATCGCGGGGAAAGAGATGATCTACCGTCAGGTAGAAAACAGCttt > 1:150881/1‑49 (MQ=255) gCCGATCGCGGGGAAAGAGATGATCTACCGTCAGGTAGAAAACAGCttt > 1:1609505/1‑49 (MQ=255) gCCGATCGCGGGGAAAGAGATGATCTACCGTCAGGTAGAAAACAGCttt > 1:3294880/1‑49 (MQ=255) gCCGATCGCGGGGAAAGAGATGATCTACCGTCAGGTAGAAAACAGCttt > 1:3095114/1‑49 (MQ=255) gCCGATCGCGGGGAAAGAGATGATCTACCGTCAGGTAGAAAACAGCttt > 1:246952/1‑49 (MQ=255) gCCGATCGCGGGGAAAGAGATGATCTACCGTCAGGTAGAAAACAGCttt > 1:3109791/1‑49 (MQ=255) gCCGATCGCGGGGAAAGAGATGATCTACCGTCAGGTAGAAAACAGCttt > 1:1678920/1‑49 (MQ=255) gCCGATCGCGGGGAAAGAGATGATCTACCGTCAGGTAGAAAACAGCtt > 1:3042887/1‑48 (MQ=255) gCCGATCGCGGGGAAAGAGATGATCTACCGTCAGGTAGAAAACAGCtt > 1:1274302/1‑48 (MQ=255) gCCGATCGCGGGGAAAGAGATGATCTACCGTCAGGTAGAAAACAGCtt > 1:2660784/1‑48 (MQ=255) ccGGTCGCAGGGAAAGAGATGATCTACCGTCAGGTAGAAAACAGCTTTACCCAGCCTAACGCGGCGATg > 1:562083/1‑69 (MQ=255) ggTCGCAGGGAAAGAGATGATCTACCGTCAGGTAGAAAACAGCTTTACCCAGCCGAACGCGGCGATGaa < 1:3277204/69‑1 (MQ=255) gTCGCAGGGAAAGAGATGATCTACCGTCAGGTAGAAAACAGCTTTACCCAGCCGAACGCGGCGATGaa < 1:1207143/68‑1 (MQ=255) | CTGATTGGTCGGGCAACGAAAACCAAAATCGAGCTGGATCAGGATTACATCGATGAACGTCTGCCGGTCGCAGGGAAAGAGATGATCTACCGTCAGGTAGAAAACAGCTTTACCCAGCCGAACGCGGCGATGAA > minE/2279570‑2279703 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |