| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,280,578 | C→T | 48.6% | L13L (CTG→CTA) | yijD ← | conserved inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,280,578 | 0 | C | T | 48.6% | 5.5 / 99.4 | 111 | L13L (CTG→CTA) | yijD | conserved inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base C (27/30); new base T (29/25); total (56/55) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.71e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.45e-01 | |||||||||||
Only 100 of 130 total aligned reads displayed.
GAAAATGGCACAATGGAGCTAAACAGCGCTGCGAAAGTACCATTAATCGATAAGCCAGCAACTAACGCCAGCAGCAGCGTACCTCTATCTTGATTTGCTTGTTTCATTACTCGTCCTTCACATTTCCCGGAAT > minE/2280510‑2280642 | gAAAATGGCACAATGGAGCTAAACAGCGCTGCGAAAGTACCATTAATCGATAAGCCAGCAACTAACGcc > 1:1804315/1‑69 (MQ=255)gAAAATGGCACAATGGAGCTAAACAGCGCTGCGAAAGTACCATTAATCGATAAGCCAGCAACTAACGcc > 1:10493/1‑69 (MQ=255)gAAAATGGCACAATGGAGCTAAACAGCGCTGCGAAAGTACCATTAATCGATAAGCCAGCAACTAACGcc > 1:2762313/1‑69 (MQ=255)gAAAATGGCACAATGGAGCTAAACAGCGCTGCGAAAGTACCATTAATCGATAAGCCAGCAACTAACGcc > 1:2667599/1‑69 (MQ=255)gAAAATGGCACAATGGAGCTAAACAGCGCTGCGAAAGTACCATTAATCGATAAGCCAGCAACTAACGcc > 1:1185847/1‑69 (MQ=255)gAAAATGGCACAATGGAGCTAAACAGCGCTGCGAAAGTACCATTAATCGATAAGCCAGCAACTAACGcc > 1:802865/1‑69 (MQ=255)gAAAATGGCACAATGGAGCTAAACAGCGCTGCGAAAGTACCATTAATCGATAAGCCAGCAACTAACGcc > 1:2608296/1‑69 (MQ=255)gAAAATGGCACAATGGAGCTAAACAGCGCTGCGAAAGTACCATTAATCGATAAGCCAGCAACTAACGcc > 1:2208257/1‑69 (MQ=255)gAAAATGGCACAATGGAGCTAAACAGCGCTGCGAAAGTACCATTAATCGATAAGCCAGCAACTAACGcc > 1:2566257/1‑69 (MQ=255)gAAAATGGCACAATGGAGCTAAACAGCGCTGCGAAAGTACCATTAATCGATAAGCCAGCAACTAACGcc > 1:2521573/1‑69 (MQ=255)gAAAATGGCACAATGGAGCTAAACAGCGCTGCGAAAGTACCATTAATCGATAAGCCAGCAACTAACGcc > 1:824722/1‑69 (MQ=255)gAAAATGGCACAATGGAGCTAAACAGCGCTGCGAAAGTACCATTAATCGATAAGCCAGCAACTAACGcc > 1:241061/1‑69 (MQ=255) caATTGAGCTAAACAGCGCTGCGAAAGTACCATTAATCGATAAGCCAGCAACTAACGCCAGCAGCAGCg < 1:2896005/69‑1 (MQ=255) caATGGAGCTAAACAGCGCTGCGAAAGTACCATTAATCGATAAGCCAGCAACTAACGCCAGCAGCAGCg < 1:3221436/69‑1 (MQ=255) caATGGAGCTAAACAGCGCTGCGAAAGTACCATTAATCGATAAGCCAGCAACTAACGCCAGCAGCAGCg < 1:1435953/69‑1 (MQ=255) caATGGAGCTAAACAGCGCTGCGAAAGTACCATTAATCGATAAGCCAGCAACTAACGCCAGCAGCAGCg < 1:1483185/69‑1 (MQ=255) caATGGAGCTAAACAGCGCTGCGAAAGTACCATTAATCGATAAGCCAGCAACTAACGCCAGCAGCAGCg < 1:1498694/69‑1 (MQ=255) caATGGAGCTAAACAGCGCTGCGAAAGTACCATTAATCGATAAGCCAGCAACTAACGCCAGCAGCAGCg < 1:1510179/69‑1 (MQ=255) caATGGAGCTAAACAGCGCTGCGAAAGTACCATTAATCGATAAGCCAGCAACTAACGCCAGCAGCAGCg < 1:495716/69‑1 (MQ=255) caATGGAGCTAAACAGCGCTGCGAAAGTACCATTAATCGATAAGCCAGCAACTAACGCCAGCAGCAGCg < 1:172302/69‑1 (MQ=255) caATGGAGCTAAACAGCGCTGCGAAAGTACCATTAATCGATAAGCCAGCAACTAACGCCAGCAGCAGCg < 1:1955056/69‑1 (MQ=255) caATGGAGCTAAACAGCGCTGCGAAAGTACCATTAATCGATAAGCCAGCAACTAACGCCAGCAGCAGCg < 1:1918405/69‑1 (MQ=255) caATGGAGCTAAACAGCGCTGCGAAAGTACCATTAATCGATAAGCCAGCAACTAACGCCAGCAGCAGCg < 1:943378/69‑1 (MQ=255) aaTGGAGCTAAACAGCGCTGCGAAAGTACCATTAATCGATAAGCCAGCAACTAACGCCAGCAGCAGCg < 1:211044/68‑1 (MQ=255) aaTGGAGCTAAACAGCGCTGCGAAAGTACCATTAATCGATAAGCCAGCAACTAACGCCAGCAGCAGCGt < 1:3300451/69‑1 (MQ=255) aaTGGAGCTAAACAGCGCTGCGAAAGTACCATTAATCGATAAGCCAGCAACTAACGCCAGCAGCAGCGt < 1:33726/69‑1 (MQ=255) aaTGGAGCTAAACAGCGCTGCGAAAGTACCATTAATCGATAAGCCAGCAACTAACGCCAGCAGCAGCGt < 1:3169657/69‑1 (MQ=255) aaTGGAGCTAAACAGCGCTGCGAAAGTACCATTAATCGATAAGCCAGCAACTAACGCCAGCAGCAGCGt < 1:3412678/69‑1 (MQ=255) aaTGGAGCTAAACAGCGCTGCGAAAGTACCATTAATCGATAAGCCAGCAACTAACGCCAGCAGCAGCGt < 1:2868306/69‑1 (MQ=255) aaTGGAGCTAAACAGCGCTGCGAAAGTACCATTAATCGATAAGCCAGCAACTAACGCCAGCAGCAGCGt < 1:1031207/69‑1 (MQ=255) aaTGGAGCTAAACAGCGCTGCGAAAGTACCATTAATCGATAAGCCAGCAACTAACGCCAGCAGCAGCGt < 1:808108/69‑1 (MQ=255) aaTGGAGCTAAACAGCGCTGCGAAAGTACCATTAATCGATAAGCCAGCAACTAACGCCAGCAGCAGCGt < 1:858993/69‑1 (MQ=255) aaTGGAGCTAAACAGCGCTGCGAAAGTACCATTAATCGATAAGCCAGCAACTAACGCCAGCAGCAGCGt < 1:136292/69‑1 (MQ=255) aTGGAGCTAAACAGCGCTGCGAAAGTACCATTAATCGATAAGCCAGCAACTAACGCCAGCAGCAGCGt < 1:2552927/68‑1 (MQ=255) tGGAGCTAAACAGCGCTGCGAAAGTACCATTAATCGATAAGCCAGCAACTAACGCCAGCAGCAGCGt < 1:293890/67‑1 (MQ=255) ggAGCTAAACAGCGCTGCGAAAGTACCATTAATCGATAAGCCAGCAACTAACGCCAGCAGCAGCGt < 1:1883398/66‑1 (MQ=255) gAGCTAAACAGCGCTGCGAAAGTACCATTAATCGATAAGCCAGCAACTAACGCCAGCAGCAGCGTAcc > 1:1757709/1‑68 (MQ=255) gAGCTAAACAGCGCTGCGAAAGTACCATTAATCGATAAGCCAGCAACTAACGCCAGCAGCAGCGTAcc > 1:1063474/1‑68 (MQ=255) gAGCTAAACAGCGCTGCGAAAGTACCATTAATCGATAAGCCAGCAACTAACGCCAGCAGCAGCGTAcc > 1:2944809/1‑68 (MQ=255) gAGCTAAACAGCGCTGCGAAAGTACCATTAATCGATAAGCCAGCAACTAACGCCAGCAGCAGCGTAcc > 1:2100903/1‑68 (MQ=255) gAGCTAAACAGCGCTGCGAAAGTACCATTAATCGATAAGCCAGCAACTAACGCCAGCAGCAGCGTAcc > 1:1273204/1‑68 (MQ=255) cAGCGCTGCGAAAGTACCATTAATCGATAAGCCAGCAACTAACGCCAGCAGCAGCGTACCTCTATCTTg > 1:791180/1‑69 (MQ=255) cAGCGCTGCGAAAGTACCATTAATCGATAAGCCAGCAACTAACGCCAGCAGCAGCGTACCTCTATCTTg > 1:2705041/1‑69 (MQ=255) cAGCGCTGCGAAAGTACCATTAATCGATAAGCCAGCAACTAACGCCAGCAGCAGCGTACCTCTATCTTg > 1:1517315/1‑69 (MQ=255) aCCATTAATCGATAAGCCAGCAACTAACGCCAGCAGCAGCGTACCTCTATCTTGAt > 1:236955/1‑56 (MQ=255) aCCATTAATCGATAAGCCAGCAACTAACGCCAGCAGCAGCGTACCTCTATCTTGATTTGCTTGTTTCAt > 1:3319149/1‑69 (MQ=255) aCCATTAATCGATAAGCCAGCAACTAACGCCAGCAGCAGCGTACCTCTATCTTGATTTGCTTGTTTCAt > 1:1548425/1‑69 (MQ=255) aCCATTAATCGATAAGCCAGCAACTAACGCCAGCAGCAGCGTACCTCTATCTTGATTTGCTTGTTTCAt > 1:2857718/1‑69 (MQ=255) aCCATTAATCGATAAGCCAGCAACTAACGCCAGCAGCAGCGTACCTCTATCTTGATTTGCTTGTTTCAt > 1:306800/1‑69 (MQ=255) aCCATTAATCGATAAGCCAGCAACTAACGCCAGCAGCAGCGTACCTCTATCTTGACTTGCTTGTTTCAt > 1:1618946/1‑69 (MQ=255) tAAGCCAGCAACTAACGCCAGCAGCAGCGTACCTCTATCTTGATTTGCTTGTTTCTTTACTCGTCCt > 1:220759/1‑67 (MQ=255) tAAGCCAGCAACTAACGCCAGCAGCAGCGTACCTCTATCTTGATTTGCTTGTTTCATTACTCGTccat > 1:1564809/1‑66 (MQ=255) tAAGCCAGCAACTAACGCCAGCAGCAGCGTACCTCTATCTTGATTTGCTTGTTTCATTACTCGTCCtt > 1:1128353/1‑68 (MQ=255) tAAGCCAGCAACTAACGCCAGCAGCAGCGTACCTCTATCTTGATTTGCTTGTTTCATTACTCGTCCtt > 1:1695095/1‑68 (MQ=255) aGCCAGCAACTAACGCCAGCAGCAGCGTACCTCTATCTTGATTTGCTTGTTTCATTACTCGTCCTTcac < 1:1410981/69‑1 (MQ=255) gCCAGCAACCAACGCTAGCAGCAGCGTACCTCTATCTTGATTTGCTTGTTTCa > 1:1396822/1‑53 (MQ=255) gCCAGCAACCAACGCTAGCAGCAGCGTACCTCTATCTTGATTTGCTTGTTTCa > 1:376978/1‑53 (MQ=255) gCCAGCAACCAACGCTAGCAGCAGCGTACCTCTATCTTGATTTGCTTGTTTCAt > 1:1042836/1‑54 (MQ=255) gCCAGCAACCAACGCTAGCAGCAGCGTACCTCTATCTTGATTTGCTTGTTTCAt > 1:3319292/1‑54 (MQ=255) gCCAGCAACCAACGCTAGCAGCAGCGTACCTCTATCTTGATTTGCTTGTTTCAt > 1:753360/1‑54 (MQ=255) gCCAGCAACCAACGCTAGCAGCAGCGTACCTCTATCTTGATTTGCTTGTTTCAt > 1:806099/1‑54 (MQ=255) gCCAGCAACCAACGCTAGCAGCAGCGTACCTCTATCTTGATTTGCTTGTTTCAt > 1:599267/1‑54 (MQ=255) gCCAGCAACCAACGCTAGCAGCAGCGTACCTCTATCTTGATTTGCTTGTTTCAt > 1:3080939/1‑54 (MQ=255) gCCAGCAACCAACGCTAGCAGCAGCGTACCTCTATCTTGATTTGCTTGTTTCAt > 1:933650/1‑54 (MQ=255) gCCAGCAACCAACGCTAGCAGCAGCGTACCTCTATCTTGATTTGCTTGTTTCAt > 1:3399730/1‑54 (MQ=255) gCCAGCAACCAACGCTAGCAGCAGCGTACCTCTATCTTGATTTGCTTGTTTCAt > 1:3350169/1‑54 (MQ=255) gCCAGCAACCAACGCTAGCAGCAGCGTACCTCTATCTTGATTTGCTTGTTTCAt > 1:2704263/1‑54 (MQ=255) gCCAGCAACCAACGCTAGCAGCAGCGTACCTCTATCTTGATTTGCTTGTTTCAt > 1:2009376/1‑54 (MQ=255) gCCAGCAACCAACGCTAGCAGCAGCGTACCTCTATCTTGATTTGCTTGTTTCAt > 1:2051377/1‑54 (MQ=255) gCCAGCAACCAACGCTAGCAGCAGCGTACCTCTATCTTGATTTGCTTGTTTCAt > 1:2240707/1‑54 (MQ=255) gCCAGCAACCAACGCTAGCAGCAGCGTACCTCTATCTTGATTTGCTTGTTTCAt > 1:180232/1‑54 (MQ=255) gCCAGCAACCAACGCTAGCAGCAGCGTACCTCTATCTTGATTTGCTTGTTTCAt > 1:172758/1‑54 (MQ=255) gCCAGCAACCAACGCTAGCAGCAGCGTACCTCTATCTTGATTTGCTTGTTTCAt > 1:2382975/1‑54 (MQ=255) gCCAGCAACCAACGCTAGCAGCAGCGTACCTCTATCTTGATTTGCTTGTTTCAt > 1:2513621/1‑54 (MQ=255) gCCAGCAACCAACGCTAGCAGCAGCGTACCTCTATCTTGATTTGCTTGTTTCAt > 1:1280451/1‑54 (MQ=255) gCCAGCAACCAACGCTAGCAGCAGCGTACCTCTATCTTGATTTGCTTGTTTCAt > 1:2658996/1‑54 (MQ=255) gCCAGCAACAAACGCTAGCAGCAGCGTACCTCTATCTTGATTTGCTTGTTTCAt > 1:3149676/1‑54 (MQ=255) caaccaacGCTAGCAGCAGCGTACCTCTATCTTGATTTGCTTGTTTCATTa < 1:1302578/51‑1 (MQ=255) caaccaacGCTAGCAGCAGCGTACCTCTATCTTGATTTGCTTGTTTCATTa < 1:1374028/51‑1 (MQ=255) caaccaacGCTAGCAGCAGCGTACCTCTATCTTGATTTGCTTGTTTCATTa < 1:860071/51‑1 (MQ=255) caaccaacGCTAGCAGCAGCGTACCTCTATCTTGATTTGCTTGTTTCATTa < 1:1640077/51‑1 (MQ=255) caaccaacGCTAGCAGCAGCGTACCTCTATCTTGATTTGCTTGTTTCATTa < 1:1237900/51‑1 (MQ=255) caaccaacGCTAGCAGCAGCGTACCTCTATCTTGATTTGCTTGTTTCATTa < 1:2777487/51‑1 (MQ=255) caaccaacGCTAGCAGCAGCGTACCTCTATCTTGATTTGCTTGTTTCATTa < 1:194286/51‑1 (MQ=255) caaccaacGCTAGCAGCAGCGTACCTCTATCTTGATTTGCTTGTTTCATTa < 1:1943042/51‑1 (MQ=255) caaccaacGCTAGCAGCAGCGTACCTCTATCTTGATTTGCTTGTTTCATTa < 1:648407/51‑1 (MQ=255) caaccaacGCTAGCAGCAGCGTACCTCTATCTTGATTTGCTTGTTTCATTa < 1:949796/51‑1 (MQ=255) caaccaacGCTAGCAGCAGCGTACCTCTATCTTGATTTGCTTGTTTCATTa < 1:52007/51‑1 (MQ=255) caaccaacGCTAGCAGCAGCGTACCTCTATCTTGATTTGCTTGTTTCATTa < 1:2009463/51‑1 (MQ=255) caaccaacGCTAGCAGCAGCGTACCTCTATCTTGATTTGCTTGTTTCATTa < 1:2083858/51‑1 (MQ=255) caaccaacGCTAGCAGCAGCGTACCTCTATCTTGATTTGCTTGTTTCATTa < 1:228569/51‑1 (MQ=255) caaccaacGCTAGCAGCAGCGTACCTCTATCTTGATTTGCTTGTTTCATTa < 1:2295751/51‑1 (MQ=255) caaccaacGCTAGCAGCAGCGTACCTCTATCTTGATTTGCTTGTTTCATTa < 1:2339110/51‑1 (MQ=255) caaccaacGCTAGCAGCAGCGTACCTCTATCTTGATTTGCTTGTTTCATTa < 1:2578779/51‑1 (MQ=255) caaccaacGCTAGCAGCAGCGTACCTCTATCTTGATTTGCTTGTTTCATTa < 1:3141152/51‑1 (MQ=255) caaccaacGCTAGCAGCAGCGTACCTCTATCTTGATTTGCTTGTTTCATTa < 1:3139124/51‑1 (MQ=255) caaccaacGCTAGCAGCAGCGTACCTCTATCTTGATTTGCTTGTTTCATTa < 1:2598593/51‑1 (MQ=255) caaccaacGCTAGCAGCAGCGTACCTCTATCTTGATTTGCTTGTTTCATTa < 1:2654709/51‑1 (MQ=255) gCCAGCAGCAGCTTACCTCTATCTTGATTTGCTTGTTTCATTACTCGTCCTTCACATTTCCCGGaat < 1:3224963/67‑1 (MQ=255) ccAGCAGCAGCGTACCTCTATCTTGATTTGCTTGTTTCATTACTCGTCCTTCACATTTCCCGGaat < 1:2129421/66‑1 (MQ=255) | GAAAATGGCACAATGGAGCTAAACAGCGCTGCGAAAGTACCATTAATCGATAAGCCAGCAACTAACGCCAGCAGCAGCGTACCTCTATCTTGATTTGCTTGTTTCATTACTCGTCCTTCACATTTCCCGGAAT > minE/2280510‑2280642 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |