| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,281,150 | C→T | 37.1% | A57A (GCG→GCA) | fabR ← | DNA‑binding transcriptional repressor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,281,150 | 0 | C | T | 37.1% | 53.7 / 51.4 | 86 | A57A (GCG→GCA) | fabR | DNA‑binding transcriptional repressor |
| Reads supporting (aligned to +/- strand): ref base C (28/26); new base T (18/14); total (46/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.24e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTCAGACCCAGTTCGTCTACGTCGCGGAAATGCCGATAAAAAGAGGTGGGAGCAATGCCCGCTTCACGCGCCACTTCACGCAAACTCAGGCTGGCGAAGCTGCGTTCAGCACTTAATTGGCTAAAT > minE/2281082‑2281207 | gTCAGACCCAGTTCGTCTACGTCGCGGAAATGCCGATAAAAAGAGGTGGGAGCAATGCCCGCTTCAcgc > 1:2766601/1‑69 (MQ=255)gTCAGACCCAGTTCGTCTACGTCGCGGAAATGCCGATAAAAAGAGGTGGGAGCAATGCCCGCTTCAcgc > 1:1534153/1‑69 (MQ=255)gTCAGACCCAGTTCGTCTACGTCGCGGAAATGCCGATAAAAAGAGGTGGGAGCAATGCCCGCTTCAcgc > 1:3173869/1‑69 (MQ=255) gTTCGTCTACGTCGCGGAAATGCCGATAAAAAGAGGTGTGAGCAATGCCTGCTTCTCGTGCCACTTCAc < 1:248123/69‑1 (MQ=255) gTTCGTCTACGTCGCGGAAATGCCGATAAAAAGAGGTGGGAGCAATGCCTGCTTCTCGTGCCACTTCAc < 1:2674651/69‑1 (MQ=255) gTTCGTCTACGTCGCGGAAATGCCGATAAAAAGAGGTGGGAGCAATGCCTGCTTCTCGTGCCACTTCAc < 1:1844131/69‑1 (MQ=255) gTTCGTCTACGTCGCGGAAATGCCGATAAAAAGAGGTGGGAGCAATGCCTGCTTCTCGTGCCACTTCAc < 1:2935989/69‑1 (MQ=255) gTTCGTCTACGTCGCGGAAATGCCGATAAAAAGAGGTGGGAGCAATGCCTGCTTCTCGTGCCACTTCAc < 1:2988387/69‑1 (MQ=255) gTTCGTCTACGTCGCGGAAATGCCGATAAAAAGAGGTGGGAGCAATGCCTGCTTCTCGTGCCACTTCAc < 1:254225/69‑1 (MQ=255) gTTCGTCTACGTCGCGGAAATGCCGATAAAAAGAGGTGGGAGCAATGCCTGCTTCTCGTGCCACTTCAc < 1:333365/69‑1 (MQ=255) gTTCGTCTACGTCGCGGAAATGCCGATAAAAAGAGGTGGGAGCAATGCCTGCTTCTCGTGCCACTTCAc < 1:465343/69‑1 (MQ=255) gTTCGTCTACGTCGCGGAAATGCCGATAAAAAGAGGTGGGAGCAATGCCTGCTTCTCGTGCCACTTCAc < 1:597528/69‑1 (MQ=255) gTTCGTCTACGTCGCGGAAATGCCGATAAAAAGAGGTGGGAGCAATGCCTGCTTCTCGTGCCACTTCAc < 1:1182003/69‑1 (MQ=255) gTTCGTCTACGTCGCGGAAATGCCGATAAAAAGAGGTGGGAGCAATGCCTGCTTCTCGTGCCACTTCAc < 1:2187115/69‑1 (MQ=255) gTTCGTCTACGTCGCGGAAATGCCGATAAAAAGAGGTGGGAGCAATGCCTGCTTCTCGTGCCACTTCAc < 1:1113631/69‑1 (MQ=255) gTTCGTCTACGTCGCGGAAATGCCGATAAAAAGAGGTGGGAGCAATGCCTGCTTCTCGTGCCACTTCAc < 1:2278607/69‑1 (MQ=255) gTTCGTCTACGTCGCGGAAATGCCGATAAAAAGAGGTGGGAGCAATGCCTGCTTCTCGTGCCACTTCAc < 1:85500/69‑1 (MQ=255) tCGTCTACGTCGCGGAAATGCCGATAAAAAGAGGTGGGAGCAATGCCCGCTTCACGCGCCACTTCAc > 1:216139/1‑67 (MQ=255) tCGTCTACGTCGCGGAAATGCCGATAAAAAGAGGTGGGAGCAATGCCCGCTTCACGCGCCACTTCACg > 1:3100874/1‑68 (MQ=255) tCGTCTACGTCGCGGAAATGCCGATAAAAAGAGGTGGGAGCAATGCCCGCTTCACGCGCCACTTCACg > 1:2598181/1‑68 (MQ=255) tCGTCTACGTCGCGGAAATGCCGATAAAAAGAGGTGGGAGCAATGCCCGCTTCACGCGCCACTTCACg > 1:632715/1‑68 (MQ=255) tCGTCTACGTCGCGGAAATGCCGATAAAAAGAGGTGGGAGCAATGCCCGCTTCACGCGCCACTTCACg > 1:2659553/1‑68 (MQ=255) tCGTCTACGTCGCGGAAAAGCCGATAAAAAGAGGTGGGAGCAATGCCCGCTTCACGCGCCACTTCACg > 1:1913589/1‑68 (MQ=255) tACGTCGCGGAAATGCCGATAAAAAGAGGTGGGAGCAATGCCTGCTTCACGCGCCACTTCACGCAAACt < 1:2372929/69‑1 (MQ=255) tACGTCGCGGAAATGCCGATAAAAAGAGGTGGGAGCAATGCCCGCTTCACGCGCCACTTCACGCaaa > 1:1915128/1‑67 (MQ=255) tACGTCGCGGAAATGCCGATAAAAAGAGGTGGGAGCAATGCCCGCTTCACGCGCCACTTCACGCaaa > 1:3052739/1‑67 (MQ=255) tACGTCGCGGAAATGCCGATAAAAAGAGGTGGGAGCAATGCCCGCTTCACGCGCCACTTCACGCaaa > 1:3162420/1‑67 (MQ=255) tACGTCGCGGAAATGCCGATAAAAAGAGGTGGGAGCAATGCCCGCTTCACGCGCCACTTCACGCaaa > 1:401290/1‑67 (MQ=255) tACGTCGCGGAAATGCCGATAAAAAGAGGTGGGAGCAATGCCCGCTTCACGCGCCACTTCACGCaaa > 1:667694/1‑67 (MQ=255) tACGTCGCGGAAATGCCGATAAAAAGAGGTGGGAGCAATGCCCGCTTCACGCGCCACTTCACGCaaa > 1:2409332/1‑67 (MQ=255) tACGTCGCGGAAATGCCGATAAAAAGAGGTGGGAGCAATGCCCGCTTCACGCGCCACTTCACGCAAACt < 1:3255355/69‑1 (MQ=255) tACGTCGCGGAAATGCCGATAAAAAGAGGTGGGAGCAATGCCCGCTTCACGCGCCACTTCACGCAAACt < 1:62201/69‑1 (MQ=255) tACGTCGCGGAAATGCCGATAAAAAGAGGTGGGAGCAATGCCCGCTTCACGCGCCACTTCACGCAAACt < 1:1039776/69‑1 (MQ=255) tACGTCGCGGAAATGCCGATAAAAAGAGGTGGGAGCAATGCCCGCTTCACGCGCCACTTCACGCAAACt < 1:2290371/69‑1 (MQ=255) tACGTCGCGGAAATGCCGATAAAAAGAGGTGGGAGCAATGCCCGCTTCACGCGCCACTTCACGCAAACt < 1:1799932/69‑1 (MQ=255) gcgGAAATGCCGATAAAAAGAGGTGGGAGCAATGCCTGCTTCTCGTGCCACTTCACGCAAACTCa > 1:556983/1‑65 (MQ=255) gcgGAAATGCCGATAAAAAGAGGTGGGAGCAATGCCTGCTTCTCGTGCCACTTCACGCAAACTCAg > 1:880160/1‑66 (MQ=255) gcgGAAATGCCGATAAAAAGAGGTGGGAGCAATGCCTGCTTCTCGTGCCACTTCACGCAAACTCAg > 1:3305416/1‑66 (MQ=255) gcgGAAATGCCGATAAAAAGAGGTGGGAGCAATGCCTGCTTCTCGTGCCACTTCACGCAAACTCAg > 1:3173422/1‑66 (MQ=255) gcgGAAATGCCGATAAAAAGAGGTGGGAGCAATGCCTGCTTCTCGTGCCACTTCACGCAAACTCAg > 1:1732697/1‑66 (MQ=255) gcgGAAATGCCGATAAAAAGAGGTGGGAGCAATGCCTGCTTCTCGTGCCACTTCACGCAAACTCAg > 1:540927/1‑66 (MQ=255) gcgGAAATGCCGATAAAAAGAGGTGGGAGCAATGCCTGCTTCTCGTGCCACTTCACGCAAACTCAg > 1:2893725/1‑66 (MQ=255) gcgGAAATGCCGATAAAAAGAGGTGGGAGCAATGCCTGCTTCTCGTGCCACTTCACGCAAACTCAg > 1:2797450/1‑66 (MQ=255) gcgGAAATGCCGATAAAAAGAGGTGGGAGCAATGCCTGCTTCTCGTGCCACTTCACGCAAACTCAg > 1:656851/1‑66 (MQ=255) gcgGAAATGCCGATAAAAAGAGGTGGGAGCAATGCCTGCTTCTCGTGCCACTTCACGCAAACTCAg > 1:850885/1‑66 (MQ=255) gcgGAAATGCCGATAAAAAGAGGTGGGAGCAATGCCTGCTTCTCGTGCCACTTCACGCAAACTCAg > 1:241400/1‑66 (MQ=255) gcgGAAATGCCGATAAAAAGAGGTGGGAGCAATGCCTGCTTCTCGTGCCACTTCACGCAAACTCAg > 1:1039487/1‑66 (MQ=255) gcgGAAATGCCGATAAAAAGAGGTGGGAGCAATGCCTGCTTCTCGTGCCACTTCACGCAAACTCAg > 1:216519/1‑66 (MQ=255) gcgGAAATGCCGATAAAAAGAGGTGGGAGCAATGCCTGCTTCTCGTGCCACTTCACGCAAACTCAg > 1:2524228/1‑66 (MQ=255) gcgGAAATGCCGATAAAAAGAGGTGGGAGCAATGCCTGCTTCTCGTGCCACTTCACGCAAACTCAg > 1:2023234/1‑66 (MQ=255) gcgGAAATGCCGATAAAAAGAGGTGGGAGCAATGCCTGCTTCTCGTGCCACTTCACGCAAACTCAg > 1:2657642/1‑66 (MQ=255) gcgGAAATGCCGATAAAAAGAGGTGGGAGCAATGCCTGCTTCTCGTGCCACTTCACGCAAACTCAg > 1:737224/1‑66 (MQ=255) gcgGAAATGCCGATAAAAAGAGGTCGGAGCAATGCCTGCTTCTCGTGCCACTTCACGCAAACTCAg > 1:1954711/1‑66 (MQ=255) gCCGATAAAAAGAGGTGGGAGCAATGCCCGCTTCACGCTCCACtt < 1:6349/45‑1 (MQ=255) gCCGATAAAAAGAGGTGGGAGCAATGCCCGCTTCACGCGCCACtt < 1:824326/45‑1 (MQ=255) gCCGATAAAAAGAGGTGGGAGCAATGCCCGCTTCACGCGCCACtt < 1:1198273/45‑1 (MQ=255) gCCGATAAAAAGAGGTGGGAGCAATGCCCGCTTCACGCGCCACtt < 1:106508/45‑1 (MQ=255) gCCGATAAAAAGAGGTGGGAGCAATGCCCGCTTCACGCGCCACtt < 1:1689145/45‑1 (MQ=255) gCCGATAAAAAGAGGTGGGAGCAATGCCCGCTTCACGCGCCACtt < 1:3112389/45‑1 (MQ=255) gCCGATAAAAAGAGGTGGGAGCAATGCCCGCTTCACGCGCCACtt < 1:2349136/45‑1 (MQ=255) ccGATAAAAAGAGGTGGGAGCAATGCGCGCTTCACGCGCCACtt < 1:2711960/44‑1 (MQ=255) cGATAAAAAGAGGTGGGAGCAATGCCCGCTTCACGCGCCACTTCACGCAAACt < 1:1310662/53‑1 (MQ=255) aaaaaGAGGTGGGAGCAATGCCCGCTTCACGCGCCACTTCACGCAAACTCAGGCTGGCGAAGCTGCg > 1:736561/1‑67 (MQ=255) aaaaGAGGTGGGAGCAATGCCCGCTTCACGCGCCACTTCACGCAAACTCAGGCTGGCGAAGCTGCGTTc > 1:1537915/1‑69 (MQ=255) aaaaGAGGTGGGAGCAATGCCCGCTTCACGCGCCACTTCACGCAAACTCAGGCTGGCGAAGCTGCGTTc > 1:3267658/1‑69 (MQ=255) aaaaGAGGTGGGAGCAATGCCCGCTTCACGCGCCACTTCACGCAAACTCAGGCTGGCGAAGCTGCGTTc > 1:2326378/1‑69 (MQ=255) aaaaGAGGTGGGAGCAATGCCCGCTTCACGCGCCACTTCACGCAAACTCAGGCTGGCGAAGCTGCGTTc > 1:3177167/1‑69 (MQ=255) aaaaGAGGTGGGAGCAATGCCCGCTTCACGCGCCACTTCACGCAAACTCAGGCTGGCGAAGCTGCGTTc > 1:1433990/1‑69 (MQ=255) aaaaGAGGTGGGAGCAATGCCCGCTTCACGCGCCACTTCACGCAAACTCAGGCTGGCGAAGCTGCGTTc > 1:2466362/1‑69 (MQ=255) aaaaGAGGTGGGAGCAATGCCCGCTTCACGCGCCACTTCACGCAAACTCAGGCTGGCGAAGCTGCGTTc > 1:2989791/1‑69 (MQ=255) aaaaGAGGTGGGAGCAATGCCCGCTTCACGCGCCACTTCACGCAAACTCAGGCTGGCGAAGCTGCGTTc > 1:2043932/1‑69 (MQ=255) aaaaGAGGTGGGAGCAATGCCCGCTTCACGCGCCACTTCACGCAAACTCAGGCTGGCGAAGCTGCGTTc > 1:2572257/1‑69 (MQ=255) aaaaGAGGTGGGAGCAATGCCCGCTTCACGCGCCACTTCACGCAAACTCAGGCTGGCGAAGCTGCGTTc > 1:1995905/1‑69 (MQ=255) aaGAGGTGGGAGCAATGCCCGCTTCACGCGCCACTTCACGCAAACTCAGGCTGGCGAAGCTGCGTTCAg < 1:3031461/69‑1 (MQ=255) aaGAGGTGGGAGCAATGCCCGCTTCACGCGCCACTTCACGCAAACTCAGGCTGGCGAAGCTGCGTTCAg < 1:1295181/69‑1 (MQ=255) aaGAGGTGGGAGCAATGCCCGCTTCACGCGCCACTTCACGCAAACTCAGGCTGGCGAAGCTGCGTTCAg < 1:3001853/69‑1 (MQ=255) aaGAGGTGGGAGCAATGCCCGCTTCACGCGCCACTTCACGCAAACTCAGGCTGGCGAAGCTGCGTTCAg < 1:1741429/69‑1 (MQ=255) aaGAGGTGGGAGCAATGCCCGCTTCACGCGCCACTTCACGCAAACTCAGGCTGGCGAAGCTGCGTTCAg < 1:802762/69‑1 (MQ=255) ggAGCAATGCCCGCTTCACGCGCCACTTCACGCAAACTCAGGCTGGCGAAGCTGCGTTCAg < 1:2687557/61‑1 (MQ=255) aTGCCCGCTTCACGCGCCACTTCACGCAAACTCAGGCTGGCGAAGCTGCGTTCAGCACTTAATTGGCTa < 1:279804/69‑1 (MQ=255) aTGCCCGCTTCACGCGCCACTTCACGCAAACTCAGGCTGGCGAAGCTGCGTTCAGCACTTAATTGGCTa < 1:760401/69‑1 (MQ=255) aTGCCCGCTTCACGCGCCACTTCACGCAAACTCAGGCTGGCGAAGCTGCGTTCAGCACTTAATTGGCTa < 1:1087859/69‑1 (MQ=255) aTGCCCGCTTCACGCGCCACTTCACGCAAACTCAGGCTGGCGAAGCTGCGTTCAGCACTTAATTGGCTa < 1:1149200/69‑1 (MQ=255) tGCCCGCTTCACGCGCCACTTCACGCAAACTCAGGCTGGCGAAGCTGCGTTCAGCACTTAATTGGCTa < 1:2772153/68‑1 (MQ=255) gCCCGCTTCACGCGCCACTTCACGCAAACTCAGGCTGGCGAAGCTGCGTTCAGCACTTAATTGGCTaaa > 1:201322/1‑69 (MQ=255) gCCCGCTTCACGCGCCACTTCACGCAAACTCAGGCTGGCGAAGCTGCGTTCAGCACTTAATTGGCTaaa > 1:747997/1‑69 (MQ=255) gCCCGCTTCACGCGCCACTTCACGCAAACTCAGGCTGGCGAAGCTGCGTTCAGCACTTAATTGGCTaaa > 1:135450/1‑69 (MQ=255) cccGCTTCACGCGCCACTTCACGCAAACTCAGGCTGGCGAAGCTGCGTTCAGCACTTAATTGGCTAAAt > 1:3207469/1‑69 (MQ=255) cccGCTTCACGCGCCACTTCACGCAAACTCAGGCTGGCGAAGCTGCGTTCAGCACTTAATTGGCTAAAt > 1:1286975/1‑69 (MQ=255) | GTCAGACCCAGTTCGTCTACGTCGCGGAAATGCCGATAAAAAGAGGTGGGAGCAATGCCCGCTTCACGCGCCACTTCACGCAAACTCAGGCTGGCGAAGCTGCGTTCAGCACTTAATTGGCTAAAT > minE/2281082‑2281207 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |