| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,281,337 | A→G | 36.2% | intergenic (‑17/‑260) | fabR ← / → sthA | DNA‑binding transcriptional repressor/pyridine nucleotide transhydrogenase, soluble |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,281,337 | 0 | A | G | 36.2% | 47.5 / 48.4 | 80 | intergenic (‑17/‑260) | fabR/sthA | DNA‑binding transcriptional repressor/pyridine nucleotide transhydrogenase, soluble |
| Reads supporting (aligned to +/- strand): ref base A (28/23); new base G (15/14); total (43/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.19e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.66e-01 | |||||||||||
TGAATCCTTGCCAAAAGTACTGCTGGCACTATACCAGAGAATGAACATACTGGATTGCTCCAGTATTGTGAATGAACGGTAACGCAATAAAACATATTTACTGCAATTCTTTGGCCGGTTCTTTT > minE/2281273‑2281397 | tGAATCCTTGCCAAAAGTACTGCTGGCACTATACCAGAGAATGAACATACTGGATTGCTCCAGTATtg > 1:2621211/1‑68 (MQ=255) tCCTTGCCAAAAGTACTGCTGGCACTATACCAGAGAATGAACATACTGGATTGCTCCAGTATtgtg < 1:548299/66‑1 (MQ=255) tCCTTGCCAAAAGTACTGCTGGCACTATACCAGAGAATGAACATACTGGATTGCTCCAGTATtgtg < 1:2641310/66‑1 (MQ=255) tCCTTGCCAAAAGTACTGCTGGCACTATACCAGAGAATGAACATACTGGATTGCTCCAGTATtgtg < 1:2718063/66‑1 (MQ=255) tCCTTGCCAAAAGTACTGCTGGCACTATACCAGAGAATGAACATACTGGATTGCTCCAGTATtgtg < 1:2734406/66‑1 (MQ=255) tCCTTGCCAAAAGTACTGCTGGCACTATACCAGAGAATGAACATACTGGATTGCTCCAGTATtgtg < 1:1636152/66‑1 (MQ=255) gCCAAATGTACTGCTGGCACTATACCAGAGAATGAACATACTGGATTGCTCCAGTGTtgtg < 1:867281/61‑1 (MQ=255) gCCAAAAGTACTGCTTGCACTATACCAGAGAATGAACATACTGGATTGCTCCAGTGTtgtg < 1:1070695/61‑1 (MQ=255) gCCAAAAGTACTGCTGTCACTATACCAGAGAATGAACATACTGGATTGCTCCAGTGTtgtg < 1:1958800/61‑1 (MQ=255) gCCAAAAGTACTGCTGGCACTATACCAGAGAATGAACATACTGGATTTCTCCAGTGTtgtg < 1:2779941/61‑1 (MQ=255) gCCAAAAGTACTGCTGGCACTATACCAGAGAATGAACATACTGGATTGCTCCAGTGTtgtg < 1:3404370/61‑1 (MQ=255) gCCAAAAGTACTGCTGGCACTATACCAGAGAATGAACATACTGGATTGCTCCAGTGTtgtg < 1:1973658/61‑1 (MQ=255) gCCAAAAGTACTGCTGGCACTATACCAGAGAATGAACATACTGGATTGCTCCAGTGTtgtg < 1:1911810/61‑1 (MQ=255) gCCAAAAGTACTGCTGGCACTATACCAGAGAATGAACATACTGGATTGCTCCAGTGTtgtg < 1:2644500/61‑1 (MQ=255) gCCAAAAGTACTGCTGGCACTATACCAGAGAATGAACATACTGGATTGCTCCAGTGTtgtg < 1:397129/61‑1 (MQ=255) gCCAAAAGTACTGCTGGCACTATACCAGAGAATGAACATACTGGATTGCTCCAGTGTtgtg < 1:475475/61‑1 (MQ=255) gCCAAAAGTACTGCTGGCACTATACCAGAGAATGAACATACTGGATTGCTCCAGTGTtgtg < 1:675879/61‑1 (MQ=255) gCCAAAAGTACTGCTGGCACTATACCAGAGAATGAACATACTGGATTGCTCCAGTGTtgtg < 1:1230890/61‑1 (MQ=255) gCCAAAAGTACTGCTGGCACTATACCAGAGAATGAACATACTGGATTGCTCCAGTGTtgtg < 1:1180640/61‑1 (MQ=255) ccAAAAGTACTGCTGGCACTATACCAGAGAATGAACATACTGGATTGCTCCAGTGTtgtg < 1:2411873/60‑1 (MQ=255) aaGTACTGCTGGCACTATACCAGAGAATGAACATACTGGATTGCTCCAGTATTGTGAATGAACGGTaa > 1:1121912/1‑68 (MQ=255) aaGTACTGCTGGCACTATACCAGAGAATGAACATACTGGATTGCTCCAGTATTGTGAATGAACGGTaa > 1:3410430/1‑68 (MQ=255) aaGTACTGCTGGCACTATACCAGAGAATGAACATACTGGATTGCTCCAGTATTGTGAATGAACGGTaa > 1:516387/1‑68 (MQ=255) aaGTACTGCTGGCACTATACCAGAGAATGAACATACTGGATTGCTCCAGTATTGTGAATGAACGGTaa > 1:1182580/1‑68 (MQ=255) aaGTACTGCTGGCACTATACCAGAGAATGAACATACTGGATTGCTCCAGTATTGTGAATGAACGGTaa > 1:142908/1‑68 (MQ=255) aaGTACTGCTGGCACTATACCAGAGAATGAACATACTGGATTGCTCCAGTATTGTGAATGAACGGTa > 1:2124185/1‑67 (MQ=255) ggCACTATACCAGAGAATGAACATACTGGATTGCTCCAGTATTGTGAATGAACGGTAACGCAATaaaa > 1:341288/1‑68 (MQ=255) aCTATACCAGAGAATGAACATACTGGATTGCTCCAGTATTGTGAATGAACGGTAACGCAATAAAACa < 1:159300/67‑1 (MQ=255) tataCCAGAGAATGAACATACTGGATTGCTCCAGTATTGTGAATGAACGGTAACGCAATAAAACATAtt < 1:165238/69‑1 (MQ=255) taCCAGAGAATGAACATACTGGATTGCTCCAGTATTGtgaatg < 1:2153472/43‑1 (MQ=255) taCCAGAGAATGAACATACTGGATTGCTCCAGTATTGtgaatg < 1:65907/43‑1 (MQ=255) ccAGAGAATGAACATACTGGATTGCTCCAGTATTGTGAATGAACGGTAACGCAATAAAACATATTTAc > 1:2954599/1‑68 (MQ=255) ccAGAGAATGAACATACTGGATTGCTCCAGTATTGTGAATGAACGGTAACGCAATAAAACATATTTAc > 1:2823617/1‑68 (MQ=255) ccAGAGAATGAACATACTGGATTGCTCCAGTATTGTGAATGAACGGTAACGCAATAAAACATATTTAc > 1:2795863/1‑68 (MQ=255) aaCATACTGGATTGCTCCAGTATTGTGAATGAACGGTAACGCAATAAAACATATTTACTGCAATTCttt > 1:2585791/1‑69 (MQ=255) aaCATACTGGATTGCTCCAGTATTGTGAATGAACGGTAACGCAATAAAACATATTTACTGCAATTCttt > 1:1892640/1‑69 (MQ=255) aCTGGATTGCTCCAGTATTGTGAATGAACGGTAACGCAATAAAACATAtt < 1:3031201/50‑1 (MQ=255) aCTGGATTGCTCCAGTATTGTGAATGAACGGTAACGCAATAAAACATAtt < 1:3296107/50‑1 (MQ=255) aCTGGATTGCTCCAGTATTGTGAATGAACGGTAACGCAATAAAACATAtt < 1:577586/50‑1 (MQ=255) aCTGGATTGCTCCAGTATTGTGAATGAACGGTAACGCAATAAAACATAtt < 1:1299936/50‑1 (MQ=255) aCTGGATTGCTCCAGTATTGTGAATGAACGGTAACGCAATAAAACATAtt < 1:209889/50‑1 (MQ=255) ggATTGCTCCAGTATTGTGAATGAACGGTAACGCAATAAAACATATTTACTGCAATTCTTTGGCCGGt < 1:683687/68‑1 (MQ=255) ggATTGCTCCAGTATTGTGAATGAACGGTAACGCAATAAAACATATTTACTGCAATTCTTTGGCCGGt < 1:81343/68‑1 (MQ=255) ttGCTCCAGTATTGTGAATGAACGGTAACGCAATAAAACATATTTACTGCAATTCTTTGGCCGGTTCt < 1:1716958/68‑1 (MQ=255) ttGCTCCAGTATTGTGAATGAACGGTAACGCAATAAAACATATTTACTGCAATTCTTTGGCCGGTTCt < 1:607396/68‑1 (MQ=255) ttGCTCCAGTATTGTGAATGAACGGTAACGCAATAAAACATATTTACTGCAATTCTTTGGCCGGTTCt < 1:1395863/68‑1 (MQ=255) ttGCTCCAGTATTGTGAATGAACGGTAACGCAATAAAACATATTTACTGCAATTCTTTGGCCGGTTCt < 1:2537900/68‑1 (MQ=255) ttGCTCCAGTATTGTGAATGAACGGTAACGCAATAAAACATATTTACTGCAATTCTTTGGCCGGTTCt < 1:1470849/68‑1 (MQ=255) ttGCTCCAGTATTGTGAATGAACGGTAACGCAATAAAACATATTTACTGCAATTCTTTGGCCGGTTCt < 1:2415125/68‑1 (MQ=255) ttGCTCCAGTATTGTGAATGAACGGTAACGCAATAAAACATATTTACTGCAATTCTTTGGCCGGTTCt < 1:3361775/68‑1 (MQ=255) tGCTCCAGTGTTGTGAATGAATGGTAACGCAATAAAACATATTTACTGCAATTCTTTGGCCGGTTCtt > 1:3053060/1‑68 (MQ=255) tGCTCCAGTGTTGTGAATGAACGGTAACGCAATAAAACATATTTACTGCGATTCTTTGGCCGGTTCtt > 1:282224/1‑68 (MQ=255) tGCTCCAGTGTTGTGAATGAACGGTAACGCAATAAAACATATTTACTGCAATTCTTTGGCCGGTTCttt > 1:911043/1‑69 (MQ=255) tGCTCCAGTGTTGTGAATGAACGGTAACGCAATAAAACATATTTACTGCAATTCTTTGGCCGGTTCttt > 1:818236/1‑69 (MQ=255) tGCTCCAGTGTTGTGAATGAACGGTAACGCAATAAAACATATTTACTGCAATTCTTTGGCCGGTTCttt > 1:2464716/1‑69 (MQ=255) tGCTCCAGTGTTGTGAATGAACGGTAACGCAATAAAACATATTTACTGCAATTCTTTGGCCGGTTCttt > 1:254287/1‑69 (MQ=255) tGCTCCAGTGTTGTGAATGAACGGTAACGCAATAAAACATATTTACTGCAATTCTTTGGCCGGTTCttt > 1:2589912/1‑69 (MQ=255) tGCTCCAGTGTTGTGAATGAACGGTAACGCAATAAAACATATTTACTGCAATTCTTTGGCCGGTTCttt > 1:3349026/1‑69 (MQ=255) tGCTCCAGTGTTGTGAATGAACGGTAACGCAATAAAACATATTTACTGCAATTCTTTGGCCGGTTCttt > 1:644365/1‑69 (MQ=255) tGCTCCAGTGTTGTGAATGAACGGTAACGCAATAAAACATATTTACTGCAATTCTTTGGCCGGTTCttt > 1:1470990/1‑69 (MQ=255) tGCTCCAGTGTTGTGAATGAACGGTAACGCAATAAAACATATTTACTGCAATTCTTTGGCCGGTTCttt > 1:2946208/1‑69 (MQ=255) tGCTCCAGTGTTGTGAATGAACGGTAACGCAATAAAACATATTTACTGCAATTCTTTGGCCGGTTCttt > 1:1927024/1‑69 (MQ=255) tGCTCCAGTGTTGTGAATGAACGGTAACGCAATAAAACATATTTACTGCAATTCTTTGGCCGGTTCttt > 1:1339253/1‑69 (MQ=255) tGCTCCAGTGTTGTGAATGAACGGTAACGCAATAAAACATATTTACTGCAATTCTTTGGCCGGTTCttt > 1:535070/1‑69 (MQ=255) tGCTCCAGTGTT‑TGAATGAACGGTAACGCAATAAAACATATTTACTGCAATTCTTTGGCCGGTTCtttt > 1:2827884/1‑69 (MQ=255) gTATTGTGAATGAACGGTACCGCAATAAAACATAtt > 1:3096398/1‑36 (MQ=255) gTATTGTGAATGAACGGTAACGCAATAAAACATAtt > 1:2102437/1‑36 (MQ=255) gTATTGTGAATGAACGGTAACGCAATAAAACATAtt > 1:448703/1‑36 (MQ=255) gTATTGTGAATGAACGGTAACGCAATAAAACATAtt > 1:425305/1‑36 (MQ=255) gTATTGTGAATGAACGGTAACGCAATAAAACATAtt > 1:1467169/1‑36 (MQ=255) gTATTGTGAATGAACGGTAACGCAATAAAACATAtt > 1:1866580/1‑36 (MQ=255) gTATTGTGAATGAACGGTAACGCAATAAAACATAtt > 1:2904937/1‑36 (MQ=255) gTATTGTGAATGAACGGTAACGCAATAAAACATAtt > 1:3001872/1‑36 (MQ=255) gTATTGTGAATGAACGGTAACGCAATAAAACATAtt > 1:3301248/1‑36 (MQ=255) gTATTGTGAATGAACGGTAACGCAATAAAACATAtt > 1:3019136/1‑36 (MQ=255) gTATTGTGAATGAACGGTAACGCAATAAAACATAtt > 1:2530726/1‑36 (MQ=255) gTATTGTGAATGAACGGTAACGCAATAAAACATAtt > 1:3130228/1‑36 (MQ=255) gTATTGTGAATGAACGGTAACGCAATAAAACATAtt > 1:3111480/1‑36 (MQ=255) gTATTGTGAATGAACGGTAACGCAATAAAACATAtt > 1:839210/1‑36 (MQ=255) gTATTGTGAATGAACGGTAACGCAATAAAACATAtt > 1:2265490/1‑36 (MQ=255) | TGAATCCTTGCCAAAAGTACTGCTGGCACTATACCAGAGAATGAACATACTGGATTGCTCCAGTATTGTGAATGAACGGTAACGCAATAAAACATATTTACTGCAATTCTTTGGCCGGTTCTTTT > minE/2281273‑2281397 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |