| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,282,451 | A→G | 24.0% | L285L (CTA→CTG) | sthA → | pyridine nucleotide transhydrogenase, soluble |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,282,451 | 0 | A | G | 24.0% | 147.8 / 40.4 | 118 | L285L (CTA→CTG) | sthA | pyridine nucleotide transhydrogenase, soluble |
| Reads supporting (aligned to +/- strand): ref base A (60/29); new base G (11/17); total (71/47) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.37e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 124 total aligned reads displayed.
TGCTCTATGCCAACGGTCGCACCGGTAATACCGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCATGTATCAGACCGCACAGCCACACGTTTACGC > minE/2282390‑2282519 | tgcTCTATGCCAACGGTCGCACCGGTAATACCGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACt < 1:1121325/68‑1 (MQ=255)tgcTCTATGCCAACGGTCGCACCGGTAATACCGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACt < 1:189039/68‑1 (MQ=255)tgcTCTATGCCAACGGTCGCACCGGTAATACCGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACt > 1:1620422/1‑68 (MQ=255)tgcTCTATGCCAACGGTCGCACCGGTAATACCGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACt < 1:2603054/68‑1 (MQ=255)tgcTCTATGCCAACGGTCGCACCGGTAATACCGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACt < 1:2746941/68‑1 (MQ=255)tgcTCTATGCCAACGGTCGCACCGGTAATACCGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACt < 1:2894626/68‑1 (MQ=255)tgcTCTATGCCAACGGTCGCACCGGTAATACCGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACt > 1:817825/1‑68 (MQ=255)tgcTCTATGCCAACGGTCGCACCGGTAATACCGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACt < 1:94930/68‑1 (MQ=255) aTGCCAACGGTCGCACCGGTAATACCGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACt < 1:2482565/62‑1 (MQ=255) ccAACGGTCGCACCGGTAATACCGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACTGACAGCcgcg > 1:3350507/1‑69 (MQ=255) cAACGGTCGCACCGGTAATACCGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACTGACAGCcgcg < 1:1909394/68‑1 (MQ=255) aCCGGTAATACCGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAg < 1:577063/69‑1 (MQ=255) aCCGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACa > 1:2120193/1‑67 (MQ=255) aCCGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAg > 1:386604/1‑68 (MQ=255) aCCGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAg > 1:207475/1‑68 (MQ=255) aCCGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGc > 1:1942940/1‑69 (MQ=255) aCCGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGc > 1:983093/1‑69 (MQ=255) aCCGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGc > 1:1817058/1‑69 (MQ=255) ccGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACt > 1:2115152/1‑38 (MQ=255) ccGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACt > 1:1371503/1‑38 (MQ=255) ccGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACt > 1:2662204/1‑38 (MQ=255) ccGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACt > 1:2406870/1‑38 (MQ=255) ccGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACt > 1:3004233/1‑38 (MQ=255) ccGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACt > 1:560409/1‑38 (MQ=255) ccGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACt > 1:564338/1‑38 (MQ=255) ccGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACt > 1:1024597/1‑38 (MQ=255) ccGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACt > 1:1184943/1‑38 (MQ=255) ccGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACt > 1:199436/1‑38 (MQ=255) ccGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCa > 1:3135152/1‑69 (MQ=255) ccGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCa > 1:2609803/1‑69 (MQ=255) ccGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCa > 1:1123254/1‑69 (MQ=255) ccGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCa < 1:910484/69‑1 (MQ=255) ccGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCa > 1:2719383/1‑69 (MQ=255) ccGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCa > 1:283333/1‑69 (MQ=255) ccGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCa > 1:3125284/1‑69 (MQ=255) ccGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCa > 1:3138336/1‑69 (MQ=255) ccGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCa > 1:1266806/1‑69 (MQ=255) ccGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCa > 1:713121/1‑69 (MQ=255) ccGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCa > 1:1819636/1‑69 (MQ=255) ccGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCa > 1:642735/1‑69 (MQ=255) ccGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCa > 1:119065/1‑69 (MQ=255) ccGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCa > 1:577187/1‑69 (MQ=255) ccGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCa > 1:630142/1‑69 (MQ=255) ccGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCa < 1:1372550/69‑1 (MQ=255) ccGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCa < 1:1791002/69‑1 (MQ=255) ccGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCa > 1:1699602/1‑69 (MQ=255) ccGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCa > 1:1923446/1‑69 (MQ=255) ccGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCa < 1:1573766/69‑1 (MQ=255) ccGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCa > 1:2003974/1‑69 (MQ=255) ccGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCa > 1:1472304/1‑69 (MQ=255) ccGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCa > 1:2296181/1‑69 (MQ=255) ccGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCa > 1:2446948/1‑69 (MQ=255) ccGATTCGCTGGCGTTACAGAACAATGGGCTAGAAACTCACAGCCGCGGACAGCTGAAGGTCAACAGCa > 1:366670/1‑69 (MQ=255) cGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCa < 1:824249/68‑1 (MQ=255) gATTCGCTGGCGTTACAGAACATTGGGCTGGAAACTGACAGTCGCGGACAGCTGAAGGTCCACAGCAt > 1:2244776/1‑68 (MQ=255) gATTCGCTGGCGTTACAGAACATTGGGCTGGAAACTGACAGTCGCGGACAGCTGAAGGTCAACAGCATg > 1:3343041/1‑69 (MQ=255) gATTCGCTGGCGTTACAGAACATTGGGCTGGAAACTGACAGTCGCGGACAGCTGAAGGTCAACAGCATg > 1:3319323/1‑69 (MQ=255) gATTCGCTGGCGTTACAGAACATTGGGCTGGAAACTGACAGTCGCGGACAGCTGAAGGTCAACAGCATg > 1:2223692/1‑69 (MQ=255) gATTCGCTGGCGTTACAGAACATTGGGCTGGAAACTGACAGTCGCGGACAGCTGAAGGTCAACAGCATg > 1:749776/1‑69 (MQ=255) gATTCGCTGGCGTTACAGAACATTGGGCTGGAAACTGACAGTCGCGGACAGCTGAAGGTCAACAGCATg > 1:691075/1‑69 (MQ=255) gATTCGCTGGCGTTACAGAACATTGGGCTGGAAACTGACAGTCGCGGACAGCTGAAGGTCAACAGCATg > 1:3016988/1‑69 (MQ=255) gATTCGCTGGCGTTACAGAACATTGGGCTGGAAACTGACAGTCGCGGACAGCTGAAGATCAACAGCATg > 1:3387132/1‑69 (MQ=255) gATTCGCTGGCGTTACAGAACATTGGGCTAGAAACTGACAGTCGCGGACAGCTGAAGGTCAACAGCATg > 1:2645496/1‑69 (MQ=255) gATTCGCTGGCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCAtt < 1:3122315/69‑2 (MQ=255) gATTCGCTGGCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCATg > 1:2017450/1‑69 (MQ=255) gATTCGCTGGCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCATg < 1:2328867/69‑1 (MQ=255) aTTCGCTGGCGTTACAGAACATTGGGCTTGAAACTGACAGTCGCGGACAGCTGAAGGTCAACAGCAt < 1:2719587/67‑1 (MQ=255) aTTCGCTGGCGTTACAGAACATTGGGCTGGAAACTGACAGTCGCGGACAGCTGAAGGTCAACAGCAt < 1:69201/67‑1 (MQ=255) aTTCGCTGGCGTTACAGAACATTGGGCTGGAAACTGACAGTCGCGGACAGCTGAAGGTCAACAGCAt < 1:722281/67‑1 (MQ=255) aTTCGCTGGCGTTACAGAACATTGGGCTGGAAACTGACAGTCGCGGACAGCTGAAGGTCAACAGCAt < 1:1953505/67‑1 (MQ=255) aTTCGCTGGCGTTACAGAACATTGGGCTGGAAACTGACAGTCGCGGACAGCTGAAGGTCAACAGCAt < 1:49989/67‑1 (MQ=255) aTTCGCTGGCGTTACAGAACATTGGGCTGGAAACTGACAGTCGCGGACAGCTGAAGGTCAACAGCAt < 1:1574590/67‑1 (MQ=255) aTTCGCTGGCGTTACAGAACATTGGGCTGGAAACTGACAGTCGCGGACAGCTGAAGGTCAACAGCAt < 1:2719716/67‑1 (MQ=255) aTTCGCTGGCGTTACAGAACATTGGGCTGGAAACTGACAGTCGCGGACAGCTGAAGGTCAACAGCAt < 1:2132150/67‑1 (MQ=255) aTTCGCTGGCGTTACAGAACATTGGGCTGGAAACTGACAGTCGCGGACAGCTGAAGGTCAACAGCAt < 1:3330031/67‑1 (MQ=255) aTTCGCTGGCGTTACAGAACATTGGGCTGGAAACTGACAGTCGCGGACAGCTGAAGGTCAACAGCAt < 1:2769052/67‑1 (MQ=255) aTTCGCTGGCGTTACAGAACATTGGGCTGGAAACTGACAGTCGCGGACAGCTGAAGGTCAACAGCAt < 1:2850878/67‑1 (MQ=255) aTTCGCTGGCGTTACAGAACATTGGGCTGGAAACTGACAGTCGCGGACAGCTGAAGGTCAACAGCAt < 1:3250217/67‑1 (MQ=255) aTTCGCTGGCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCATg < 1:777012/68‑1 (MQ=255) aTTCGCTGGCGTTACAGAACATT‑GGCTGGAAACTGACAGTCGCGGACAGCTGAAGGTCAACAGCAt < 1:244392/66‑1 (MQ=255) ttCGCTGGCGTTACAGAACATTGGGCTGGAAACTGACAGTCGCGGACAGCTGAAGGTCAACAGCAt < 1:1442045/66‑1 (MQ=255) gCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCATGTATCAGAc > 1:2552723/1‑68 (MQ=255) gCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCATGTATCAGAc > 1:2873277/1‑68 (MQ=255) gCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCATGTATCAGAc > 1:2978637/1‑68 (MQ=255) gCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCATGTATCAGAc > 1:631828/1‑68 (MQ=255) gCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCATGTATCAGAc > 1:627970/1‑68 (MQ=255) gCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCATGTATCAGAc > 1:2678597/1‑68 (MQ=255) gCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCATGTATCAGAc > 1:401456/1‑68 (MQ=255) gCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCATGAATCAGAc > 1:2209255/1‑68 (MQ=255) tACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCATGTATCAGACCGcac < 1:2361540/69‑1 (MQ=255) tACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCATGTATCAGACCGcac < 1:1234773/69‑1 (MQ=255) aCAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCATGTATCAGACCGcac < 1:864237/68‑1 (MQ=255) cATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCATGTATCAGACCGCACAGCcaca > 1:129210/1‑69 (MQ=255) cATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCATGTATCAGACCGCACAGCcaca > 1:1349185/1‑69 (MQ=255) ttGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCATGTATCAGACCGCACAGCCACACg < 1:202845/69‑1 (MQ=255) gggCTAGAAACTGACAGCCGCGGACAGCTGAAGGTAAACAGCATGTATCAGACCGCACAGCCACACGtt < 1:306008/69‑1 (MQ=255) aGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCATGTATCAGACCGCACAGCCACACGTTTAcgc < 1:2664898/69‑1 (MQ=255) aGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCATGTATCAGACCGCACAGCCACACGTTTAcgc < 1:3298825/69‑1 (MQ=255) aGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCATGTATCAGACCGCACAGCCACACGTTTAcgc < 1:404260/69‑1 (MQ=255) aGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCATGTATCAGACCGCACAGCCACACGTTTAcgc < 1:3369881/69‑1 (MQ=255) | TGCTCTATGCCAACGGTCGCACCGGTAATACCGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCATGTATCAGACCGCACAGCCACACGTTTACGC > minE/2282390‑2282519 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |