| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,282,463 | C→T | 24.8% | S289S (AGC→AGT) | sthA → | pyridine nucleotide transhydrogenase, soluble |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,282,463 | 0 | C | T | 24.8% | 153.9 / 41.2 | 120 | S289S (AGC→AGT) | sthA | pyridine nucleotide transhydrogenase, soluble |
| Reads supporting (aligned to +/- strand): ref base C (51/39); new base T (12/18); total (63/57) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.41e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 120 total aligned reads displayed.
CCAACGGTCGCACCGGTAATACCGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCATGTATCAGACCGCACAGCCACACGTTTACGCGGTGGGC > minE/2282399‑2282526 | ccAACGGTCGCACCGGTAATACCGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACTGACAGCcgcg > 1:3350507/1‑69 (MQ=255) cAACGGTCGCACCGGTAATACCGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACTGACAGCcgcg < 1:1909394/68‑1 (MQ=255) aCCGGTAATACCGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAg < 1:577063/69‑1 (MQ=255) aaTACCGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAAc > 1:1559975/1‑69 (MQ=255) aCCGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAg > 1:207475/1‑68 (MQ=255) aCCGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAg > 1:386604/1‑68 (MQ=255) aCCGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGc > 1:983093/1‑69 (MQ=255) aCCGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGc > 1:1942940/1‑69 (MQ=255) aCCGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGc > 1:1817058/1‑69 (MQ=255) aCCGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGc > 1:2217606/1‑69 (MQ=255) ccGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCa > 1:3138336/1‑69 (MQ=255) ccGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCa > 1:3125284/1‑69 (MQ=255) ccGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCa > 1:577187/1‑69 (MQ=255) ccGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCa > 1:2719383/1‑69 (MQ=255) ccGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCa > 1:2609803/1‑69 (MQ=255) ccGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCa > 1:2446948/1‑69 (MQ=255) ccGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCa > 1:2296181/1‑69 (MQ=255) ccGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCa > 1:630142/1‑69 (MQ=255) ccGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCa > 1:642735/1‑69 (MQ=255) ccGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCa > 1:713121/1‑69 (MQ=255) ccGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCa < 1:910484/69‑1 (MQ=255) ccGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCa > 1:2003974/1‑69 (MQ=255) ccGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCa > 1:1123254/1‑69 (MQ=255) ccGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCa > 1:1472304/1‑69 (MQ=255) ccGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCa < 1:1573766/69‑1 (MQ=255) ccGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCa < 1:1372550/69‑1 (MQ=255) ccGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCa > 1:119065/1‑69 (MQ=255) ccGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCa < 1:1791002/69‑1 (MQ=255) ccGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCa > 1:1819636/1‑69 (MQ=255) ccGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCa > 1:1923446/1‑69 (MQ=255) ccGATTCGCTGGCGTTACAGAACAATGGGCTAGAAACTCACAGCCGCGGACAGCTGAAGGTCAACAGCa > 1:366670/1‑69 (MQ=255) gATTCGCTGGCGTTACAGAACATTGGGCTGGAAACTGACAGTcgcg > 1:121680/1‑46 (MQ=255) gATTCGCTGGCGTTACAGAACATTGGGCTGGAAACTGACAGTCGCGGACAGCTGAAGGTCCACAGCAt > 1:2244776/1‑68 (MQ=255) gATTCGCTGGCGTTACAGAACATTGGGCTGGAAACTGACAGTCGCGGACAGCTGAAGGTCAACAGCATg > 1:3016988/1‑69 (MQ=255) gATTCGCTGGCGTTACAGAACATTGGGCTGGAAACTGACAGTCGCGGACAGCTGAAGGTCAACAGCATg > 1:2223692/1‑69 (MQ=255) gATTCGCTGGCGTTACAGAACATTGGGCTGGAAACTGACAGTCGCGGACAGCTGAAGGTCAACAGCATg > 1:691075/1‑69 (MQ=255) gATTCGCTGGCGTTACAGAACATTGGGCTGGAAACTGACAGTCGCGGACAGCTGAAGGTCAACAGCATg > 1:2352545/1‑69 (MQ=255) gATTCGCTGGCGTTACAGAACATTGGGCTGGAAACTGACAGTCGCGGACAGCTGAAGGTCAACAGCATg > 1:3343041/1‑69 (MQ=255) gATTCGCTGGCGTTACAGAACATTGGGCTGGAAACTGACAGTCGCGGACAGCTGAAGGTCAACAGCATg > 1:2974612/1‑69 (MQ=255) gATTCGCTGGCGTTACAGAACATTGGGCTGGAAACTGACAGTCGCGGACAGCTGAAGGTCAACAGCATg > 1:749776/1‑69 (MQ=255) gATTCGCTGGCGTTACAGAACATTGGGCTGGAAACTGACAGTCGCGGACAGCTGAAGGTCAACAGCATg > 1:3319323/1‑69 (MQ=255) gATTCGCTGGCGTTACAGAACATTGGGCTGGAAACTGACAGTCGCGGACAGCTGAAGATCAACAGCATg > 1:3387132/1‑69 (MQ=255) gATTCGCTGGCGTTACAGAACATTGGGCTAGAAACTGACAGTCGCGGACAGCTGAAGGTCAACAGCATg > 1:2645496/1‑69 (MQ=255) gATTCGCTGGCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCAtt < 1:3122315/69‑2 (MQ=255) gATTCGCTGGCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCATg < 1:2328867/69‑1 (MQ=255) gATTCGCTGGCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCATg > 1:2017450/1‑69 (MQ=255) aTTCGCTGGCGTTACAGAACATTGGGCTGGAAACTGACAGTCGCGGACAGCTGAAGGTCAACAGCAt < 1:2850878/67‑1 (MQ=255) aTTCGCTGGCGTTACAGAACATTGGGCTGGAAACTGACAGTCGCGGACAGCTGAAGGTCAACAGCAt < 1:722281/67‑1 (MQ=255) aTTCGCTGGCGTTACAGAACATTGGGCTGGAAACTGACAGTCGCGGACAGCTGAAGGTCAACAGCAt < 1:2769052/67‑1 (MQ=255) aTTCGCTGGCGTTACAGAACATTGGGCTGGAAACTGACAGTCGCGGACAGCTGAAGGTCAACAGCAt < 1:1953505/67‑1 (MQ=255) aTTCGCTGGCGTTACAGAACATTGGGCTGGAAACTGACAGTCGCGGACAGCTGAAGGTCAACAGCAt < 1:2719716/67‑1 (MQ=255) aTTCGCTGGCGTTACAGAACATTGGGCTGGAAACTGACAGTCGCGGACAGCTGAAGGTCAACAGCAt < 1:601959/67‑1 (MQ=255) aTTCGCTGGCGTTACAGAACATTGGGCTGGAAACTGACAGTCGCGGACAGCTGAAGGTCAACAGCAt < 1:3250217/67‑1 (MQ=255) aTTCGCTGGCGTTACAGAACATTGGGCTGGAAACTGACAGTCGCGGACAGCTGAAGGTCAACAGCAt < 1:1574590/67‑1 (MQ=255) aTTCGCTGGCGTTACAGAACATTGGGCTGGAAACTGACAGTCGCGGACAGCTGAAGGTCAACAGCAt < 1:24522/67‑1 (MQ=255) aTTCGCTGGCGTTACAGAACATTGGGCTGGAAACTGACAGTCGCGGACAGCTGAAGGTCAACAGCAt < 1:49989/67‑1 (MQ=255) aTTCGCTGGCGTTACAGAACATTGGGCTGGAAACTGACAGTCGCGGACAGCTGAAGGTCAACAGCAt < 1:2132150/67‑1 (MQ=255) aTTCGCTGGCGTTACAGAACATTGGGCTGGAAACTGACAGTCGCGGACAGCTGAAGGTCAACAGCAt < 1:2104173/67‑1 (MQ=255) aTTCGCTGGCGTTACAGAACATTGGGCTGGAAACTGACAGTCGCGGACAGCTGAAGGTCAACAGCAt < 1:1352220/67‑1 (MQ=255) aTTCGCTGGCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCATg < 1:777012/68‑1 (MQ=255) ttCGCTGGCGTTACAGAACATTGGGCTGGAAACTGACAGTCGCGGACAGCTGAAGGTCAACAGCAt < 1:1442045/66‑1 (MQ=255) gCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCATGTATCAGAc > 1:401456/1‑68 (MQ=255) gCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCATGTATCAGAc > 1:3109562/1‑68 (MQ=255) gCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCATGTATCAGAc > 1:2978637/1‑68 (MQ=255) gCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCATGTATCAGAc > 1:2873277/1‑68 (MQ=255) gCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCATGTATCAGAc > 1:2678597/1‑68 (MQ=255) gCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCATGTATCAGAc > 1:2552723/1‑68 (MQ=255) gCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCATGTATCAGAc > 1:631828/1‑68 (MQ=255) gCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCATGAATCAGAc > 1:2209255/1‑68 (MQ=255) tACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCATGTATCAGACCGcac < 1:723578/69‑1 (MQ=255) tACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCATGTATCAGACCGcac < 1:851940/69‑1 (MQ=255) tACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCATGTATCAGACCGcac < 1:2361540/69‑1 (MQ=255) tACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCATGTATCAGACCGcac < 1:1234773/69‑1 (MQ=255) aCAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCATGTATCAGACCGcac < 1:864237/68‑1 (MQ=255) cATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCATGTATCAGACCGCACAGCcaca > 1:2798382/1‑69 (MQ=255) cATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCATGTATCAGACCGCACAGCcaca > 1:1349185/1‑69 (MQ=255) cATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCATGTATCAGACCGCACAGCcaca > 1:129210/1‑69 (MQ=255) ttGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCATGTATCAGACCGCACAGCCACACg < 1:68519/69‑1 (MQ=255) ttGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCATGTATCAGACCGCACAGCCACACg < 1:202845/69‑1 (MQ=255) tGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCATGTATCAGACCGCACAGCCACACg < 1:1762642/68‑1 (MQ=255) aGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCATGTATCAGACCGCACAGCCACACGTTTAcgc < 1:404260/69‑1 (MQ=255) aGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCATGTATCAGACCGCACAGCCACACGTTTAcgc < 1:3369881/69‑1 (MQ=255) aGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCATGTATCAGACCGCACAGCCACACGTTTAcgc < 1:3330413/69‑1 (MQ=255) aGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCATGTATCAGACCGCACAGCCACACGTTTAcgc < 1:3298825/69‑1 (MQ=255) aGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCATGTATCAGACCGCACAGCCACACGTTTAcgc < 1:2685456/69‑1 (MQ=255) aGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCATGTATCAGACCGCACAGCCACACGTTTAcgc < 1:2664898/69‑1 (MQ=255) aCTGACAGCCGCGGACAGCTGAAGGTCAACAGCATGTAt < 1:84420/39‑1 (MQ=255) aCTGACAGCCGCGGACAGCTGAAGGTCAACAGCATGTAt > 1:1104368/1‑39 (MQ=255) aCTGACAGCCGCGGACAGCTGAAGGTCAACAGCATGTAt < 1:311376/39‑1 (MQ=255) aCTGACAGCCGCGGACAGCTGAAGGTCAACAGCATGTAt < 1:627786/39‑1 (MQ=255) aCTGACAGCCGCGGACAGCTGAAGGTCAACAGCATGTAt < 1:1307995/39‑1 (MQ=255) aCTGACAGCCGCGGACAGCTGAAGGTCAACAGCATGTAt < 1:572095/39‑1 (MQ=255) aCTGACAGCCGCGGACAGCTGAAGGTCAACAGCATGTAt > 1:3321565/1‑39 (MQ=255) aCTGACAGCCGCGGACAGCTGAAGGTCAACAGCATGTAt < 1:3132747/39‑1 (MQ=255) aCTGACAGCCGCGGACAGCTGAAGGTCAACAGCATGTAt < 1:2783228/39‑1 (MQ=255) aCTGACAGCCGCGGACAGCTGAAGGTCAACAGCATGTAt < 1:2487736/39‑1 (MQ=255) aCTGACAGCCGCGGACAGCTGAAGGTCAACAGCATGTAt > 1:2304651/1‑39 (MQ=255) aCTGACAGCCGCGGACAGCTGAAGGTCAACAGCATGTAt > 1:1781874/1‑39 (MQ=255) aCTGACAGCCGCGGACAGCTGAAGGTCAACAGCATGTAt < 1:1961938/39‑1 (MQ=255) gACAGCCGCGGACAGCTGAAGGTCAACAGCATGTATCAGACCGCACAGCCACACGTTTACGCGGTGGGc < 1:2799785/69‑1 (MQ=255) | CCAACGGTCGCACCGGTAATACCGATTCGCTGGCGTTACAGAACATTGGGCTAGAAACTGACAGCCGCGGACAGCTGAAGGTCAACAGCATGTATCAGACCGCACAGCCACACGTTTACGCGGTGGGC > minE/2282399‑2282526 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |