| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,284,209 | C→T | 100% | Q443Q (CAG→CAA) | argH ← | argininosuccinate lyase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,284,209 | 0 | C | T | 87.7% | 26.2 / ‑3.0 | 16 | Q443Q (CAG→CAA) | argH | argininosuccinate lyase |
| Reads supporting (aligned to +/- strand): ref base C (1/1); new base T (14/0); total (15/1) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.25e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CTTACCCTAACCGAGCCTGCGCAAAAGCAATCGCCTGCGCCACCTGCTGCGGTGAGACGCCGCCTTTTGCCGCACGCTTGTCGAGGCACGATTGCAGCGACAGAATCGGATAGA > minE/2284163‑2284276 | cTTACCCTAACCGAGCCTGCGCAAAAGCAATCGCCTGCGCCACCTGCTGCGGTGAGACGCCGCCTTTTg > 1:254833/1‑69 (MQ=255) cAAAAGCAATCGCCTGCGCCACCTGCTGCGGTGAGACGCCGCCTTTTGCCGCACGCTTGTCGAGGCAc < 1:2862930/68‑1 (MQ=255) aTCGCCTGCGCCACCTGTTGCGGTGAGACACCGCCTTTTGCTGCACGCTTGTCGAGGCACGATTGCatc > 1:991879/1‑67 (MQ=255) aTCGCCTGCGCCACCTGTTGCGGTGAGACACCGCCTTTTGCTGCACGCTTGTCGAGGCACGATTGCAg > 1:2677004/1‑68 (MQ=255) aTCGCCTGCGCCACCTGTTGCGGTGAGACACCGCCTTTTGCTGCACGCTTGTCGAGGCACGATTGCAGc > 1:996162/1‑69 (MQ=255) aTCGCCTGCGCCACCTGTTGCGGTGAGACACCGCCTTTTGCTGCACGCTTGTCGAGGCACGATTGCAGc > 1:461595/1‑69 (MQ=255) aTCGCCTGCGCCACCTGTTGCGGTGAGACACCGCCTTTTGCTGCACGCTTGTCGAGGCACGATTGCAGc > 1:279426/1‑69 (MQ=255) aTCGCCTGCGCCACCTGTTGCGGTGAGACACCGCCTTTTGCTGCACGCTTGTCGAGGCACGATTGCAGc > 1:2784131/1‑69 (MQ=255) aTCGCCTGCGCCACCTGTTGCGGTGAGACACCGCCTTTTGCTGCACGCTTGTCGAGGCACGATTGCAGc > 1:2570746/1‑69 (MQ=255) aTCGCCTGCGCCACCTGTTGCGGTGAGACACCGCCTTTTGCTGCACGCTTGTCGAGGCACGATTGCAGc > 1:252197/1‑69 (MQ=255) aTCGCCTGCGCCACCTGTTGCGGTGAGACACCGCCTTTTGCTGCACGCTTGTCGAGGCACGATTGCAGc > 1:1464303/1‑69 (MQ=255) aTCGCCTGCGCCACCTGTTGCGGTGAGACACCGCCTTTTGCTGCACGCTTGTCGAGGCACGATTGCAGc > 1:2130950/1‑69 (MQ=255) aTCGCCTGCGCCACCTGTTGCGGTGAGACACCGCCTTTTGCTGCACGCTTGTCGAGGCACGATTGCAGc > 1:208357/1‑69 (MQ=255) aTCGCCTGCGCCACCTGTTGCGGTGAGACACCGCCTTTTGCTGCACGCTTGTCGAGGCACGATTGCAGc > 1:1937347/1‑69 (MQ=255) aTCGCCTGCGCCACCTGTTGCGGTGAGACACCGCCTTTTGCTGCACGCTTGTCGAGGCACGATTGCAGc > 1:189527/1‑69 (MQ=255) aTCGCCTGCGCCACCTGTTGCGGTGAGACACCGCCTTTTGCTGCACGCTTGTCGAGGCACGATTGCAGc > 1:1686583/1‑69 (MQ=255) tgctgcGGTGAGACGCCGCCTTTTGCCGCACGCTTGTCGAGGCACGATTGCAGCGACAGAATCGGATAg < 1:1033463/69‑1 (MQ=255) tgctgcGGTGAGACGCCGCCTTTTGCCGCACGCTTGTCGAGGCACGATTGCAGCGACAGAATCGGATAg < 1:3200474/69‑1 (MQ=255) tgctgcGGTGAGACGCCGCCTTTTGCCGCACGCTTGTCGAGGCACGATTGCAGCGACAGAATCGGATAg < 1:657041/69‑1 (MQ=255) gctgcGGTGAGACGCCGCCTTTTGCCGCACGCTTGTCGAGGCACGATTGCAGCGACAGAATCGGATa > 1:532664/1‑67 (MQ=255) gctgcGGTGAGACGCCGCCTTTTGCCGCACGCTTGTCGAGGCACGATTGCAGCGACAGAATCGGATAGa > 1:3207839/1‑69 (MQ=255) gctgcGGTGAGACGCCGCCTTTTGCCGCACGCTTGTCGAGGCACGATTGCAGCGACAGAATCGGATAGa < 1:991316/69‑1 (MQ=255) gctgcGGTGAGACGCCGCCTTTTGCCGCACGCTTGTCGAGGCACGATTGCAGCGACAGAATCGGATAGa < 1:915590/69‑1 (MQ=255) gctgcGGTGAGACGCCGCCTTTTGCCGCACGCTTGTCGAGGCACGATTGCAGCGACAGAATCGGATAGa > 1:68767/1‑69 (MQ=255) gctgcGGTGAGACGCCGCCTTTTGCCGCACGCTTGTCGAGGCACGATTGCAGCGACAGAATCGGATAGa > 1:56799/1‑69 (MQ=255) gctgcGGTGAGACGCCGCCTTTTGCCGCACGCTTGTCGAGGCACGATTGCAGCGACAGAATCGGATAGa > 1:149057/1‑69 (MQ=255) gctgcGGTGAGACGCCGCCTTTTGCCGCACGCTTGTCGAGGCACGATTGCAGCGACAGAATCGGATAGa < 1:370791/69‑1 (MQ=255) gctgcGGTGAGACGCCGCCTTTTGCCGCACGCTTGTCGAGGCACGATTGCAGCGACAGAATCGGATAGa < 1:3315497/69‑1 (MQ=255) gctgcGGTGAGACGCCGCCTTTTGCCGCACGCTTGTCGAGGCACGATTGCAGCGACAGAATCGGATAGa < 1:2159162/69‑1 (MQ=255) gctgcGGTGAGACGCCGCCTTTTGCCGCACGCTTGTCGAGGCACGATTGCAGCGACAGAATCGGATAGa > 1:1524802/1‑69 (MQ=255) gctgcGGTGAGACGCCGCCTTTTGCCGCACGCTTGTCGAGGCACGATTGCAGCGACAGAATCGGATAGa > 1:250957/1‑69 (MQ=255) gctgcGGTGAGACGCCGCCTTTTGCCGCACGCTTGTCGAGGCACGATTGCAGCGACAGAATCGGATAGa > 1:233762/1‑69 (MQ=255) gctgcGGTGAGACGCCGCCTTTTGCCGCACGCTTGTCGAGGCACGATTGCAGCGACAGAATCGGATAGa > 1:2319590/1‑69 (MQ=255) gctgcGGTGAGACGCCGCCCTTTGCCGCACGCTTGTCGAGGCACGATTGCAGCGACAGAATCGGATAGa > 1:2005020/1‑69 (MQ=255) | CTTACCCTAACCGAGCCTGCGCAAAAGCAATCGCCTGCGCCACCTGCTGCGGTGAGACGCCGCCTTTTGCCGCACGCTTGTCGAGGCACGATTGCAGCGACAGAATCGGATAGA > minE/2284163‑2284276 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |