BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,285,268	G→A	41.5%	G90G (GGC→GGT)	argH ←	argininosuccinate lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,285,268	0	G	A	41.5%	41.4 / 71.8	101	G90G (GGC→GGT)	argH	argininosuccinate lyase
Reads supporting (aligned to +/- strand): ref base G (31/28); new base A (25/17); total (56/45)
Fisher's exact test for biased strand distribution p-value = 5.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01

Only 100 of 115 total aligned reads displayed.

CATTACGGCTACGCCCGGTATGCAGCTTTTTGCCTAACTGGCCCACTTTGTCGATCAGTTTGCCTTCCACCCAGCTATGGATATCTTCGGCGTCGCTTTCAAGGATTTGTTGTGGCCTGGCGCGAAC  >  minE/2285207‑2285333

cATTACGGCTACGCCCTGTATGCAGCTTTTTGCCTAACTGGCCCACTTTGTCGATCAGTTTg                                                                   <  1:1973606/62‑1 (MQ=255)

cATTACGGCTACGCCCGGTATGCAGCTTTTTGCCTAACTGGCCCACTTTGTCGATCAGTTTg                                                                   >  1:1322514/1‑62 (MQ=255)

cATTACGGCTACGCCCGGTATGCAGCTTTTTGCCTAACTGGCCCACTTTGTCGATCAGTTTg                                                                   >  1:1771661/1‑62 (MQ=255)

cATTACGGCTACGCCCGGTATGCAGCTTTTTGCCTAACTGGCCCACTTTGTCGATCAGTTTg                                                                   <  1:1773945/62‑1 (MQ=255)

cATTACGGCTACGCCCGGTATGCAGCTTTTTGCCTAACTGGCCCACTTTGTCGATCAGTTTg                                                                   <  1:1472508/62‑1 (MQ=255)

cATTACGGCTACGCCCGGTATGCAGCTTTTTGCCTAACTGGCCCACTTTGTCGATCAGTTTg                                                                   >  1:2008687/1‑62 (MQ=255)

cATTACGGCTACGCCCGGTATGCAGCTTTTTGCCTAACTGGCCCACTTTGTCGATCAGTTTg                                                                   >  1:1375777/1‑62 (MQ=255)

cATTACGGCTACGCCCGGTATGCAGCTTTTTGCCTAACTGGCCCACTTTGTCGATCAGTTTg                                                                   <  1:2306316/62‑1 (MQ=255)

cATTACGGCTACGCCCGGTATGCAGCTTTTTGCCTAACTGGCCCACTTTGTCGATCAGTTTg                                                                   >  1:1018138/1‑62 (MQ=255)

cATTACGGCTACGCCCGGTATGCAGCTTTTTGCCTAACTGGCCCACTTTGTCGATCAGTTTg                                                                   <  1:934650/62‑1 (MQ=255)

cATTACGGCTACGCCCGGTATGCAGCTTTTTGCCTAACTGGCCCACTTTGTCGATCAGTTTg                                                                   >  1:360988/1‑62 (MQ=255)

    aCGGCTACGCCCGGTATGCAGCTTTTTGCCTAACTGGCCCACTTTGTCGATCAGTTTGCCTTCCACCCa                                                        >  1:1603033/1‑69 (MQ=255)

    aCGGCTACGCCCGGTATGCAGCTTTTTGCCTAACTGGCCCACTTTGTCGATCAGTTTGCCTTCCACCCa                                                        >  1:1528286/1‑69 (MQ=255)

    aCGGCTACGCCCGGTATGCAGCTTTTTGCCTAACTGGCCCACTTTGTCGATCAGTTTGCCTTCCACCCa                                                        >  1:1513840/1‑69 (MQ=255)

    aCGGCTACGCCCGGTATGCAGCTTTTTGCCTAACTGGCCCACTTTGTCGATCAGTTTGCCTTCCACCCa                                                        >  1:474381/1‑69 (MQ=255)

             cccGGTATGCAGCTTTTTGCCTAACTGGCCCACTTTGTCGATCAGTTTGCCTTCCACCCAGCTATGGat                                               <  1:3254655/69‑1 (MQ=255)

             cccGGTATGCAGCTTTTTGCCTAACTGGCCCACTTTGTCGATCAGTTTGCCTTCCACCCAGCTATGGat                                               <  1:3363068/69‑1 (MQ=255)

             cccGGTATGCAGCTTTTTGCCTAACTGGCCCACTTTGTCGATCAGTTTGCCTTCCACCCAGCTATGGat                                               <  1:2614442/69‑1 (MQ=255)

                      cAGCTTTTTGCCTAACTGGCCCACTTTGTCGATCAGTTTGCCTTCCACCCAGc                                                      >  1:2116337/1‑53 (MQ=255)

                      cAGCTTTTTGCCTAACTGGCCCACTTTGTCGATCAGTTTGCCTTCCACCCAGc                                                      >  1:46870/1‑53 (MQ=255)

                      cAGCTTTTTGCCTAACTGGCCCACTTTGTCGATCAGTTTGCCTTCCACCCAGc                                                      >  1:1311169/1‑53 (MQ=255)

                      cAGCTTTTTGCCTAACTGGCCCACTTTGTCGATCAGTTTGCCTTCCACCCAGc                                                      >  1:921956/1‑53 (MQ=255)

                      cAGCTTTTTGCCTAACTGGCCCACTTTGTCGATCAGTTTGCCTTCCACCCAGc                                                      >  1:1852992/1‑53 (MQ=255)

                      cAGCTTTTTGCCTAACTGGCCCACTTTGTCGATCAGTTTGCCTTCCACCCAGc                                                      >  1:2835264/1‑53 (MQ=255)

                      cAGCTTTTTGCCTAACTGGCCCACTTTGTCGATCAGTTTGCCTTCCACCCAGc                                                      >  1:1216171/1‑53 (MQ=255)

                      cAGCTTTTTGCCTAACTGGCCCACTTTGTCGATCAGTTTGCCTTCCACCCAGCTATGGATATCTTCGGc                                      >  1:2230990/1‑69 (MQ=255)

                      cAGCTTTTTGCCTAACTGGCCCACTTTGTCGATCAGTTTGCCTTCCACCCAGCTATGGATATCTTCGGc                                      >  1:111065/1‑69 (MQ=255)

                           ttttGCCTAACTGGCCCACTTTGTCGATCAGTTTGCCTTCCACCCAGCTATGGATATCTTCGGCGTctc                                 <  1:455246/69‑3 (MQ=255)

                           ttttGCCTAACTGGCCCACTTTGTCGATCAGTTTGCCTTCCACCCAGCTATGGATATCTTCGGCGTCGc                                 <  1:1741964/69‑1 (MQ=255)

                           ttttGCCTAACTGGCCCACTTTGTCGATCAGTTTGCCTTCCACCCAGCTATGGATATCTTCGGCGTCGc                                 <  1:3076713/69‑1 (MQ=255)

                           ttttGCCTAACTGGCCCACTTTGTCGATCAGTTTGCCTTCCACCCAGCTATGGATATCTTCGGCGTCGc                                 <  1:3123544/69‑1 (MQ=255)

                           ttttGCCTAACTGGCCCACTTTGTCGATCAGTTTGCCTTCCACCCAGCTATGGATATCTTCGGCGTCGc                                 <  1:421216/69‑1 (MQ=255)

                                 cTAACTGGCCCACTTTGTCGATCAGTTTGCCTTCCACCCAGCTATGGATATCTTCGGCGTCCCTTTCa                            <  1:3244497/68‑1 (MQ=255)

                                   aaCTGACCCACTTTGTCGATCAGTTTACCTTCGACCCAGCTATGGATATCTTCGGCGTCGCTTTCAAgg                         >  1:6612/1‑69 (MQ=255)

                                   aaCTGACCCACTTTGTCGATCAGTTTACCTTCCACCCAGCTATGGATATCTTCgg                                       >  1:480725/1‑55 (MQ=255)

                                   aaCTGACCCACTTTGTCGATCAGTTTACCTTCCACCCAGCTATGGATATCTTCGGCGTCGCTTTCAAgg                         >  1:1254747/1‑69 (MQ=255)

                                   aaCTGACCCACTTTGTCGATCAGTTTACCTTCCACCCAGCTATGGATATCTTCGGCGTCGCTTTCAAgg                         >  1:1167927/1‑69 (MQ=255)

                                   aaCTGACCCACTTTGTCGATCAGTTTACCTTCCACCCAGCTATGGATATCTTCGGCGTCGCTTTCAAgg                         >  1:2627555/1‑69 (MQ=255)

                                   aaCTGACCCACTTTGTCGATCAGTTTACCTTCCACCCAGCTATGGATATCTTCGGCGTCGCTTTCAAgg                         >  1:3038977/1‑69 (MQ=255)

                                   aaCTGACCCACTTTGTCGATCAGTTTACCTTCCACCCAGCTATGGATATCTTCGGCGTCGCTTTCAAgg                         >  1:666617/1‑69 (MQ=255)

                                   aaCTGACCCACTTTGTCGATCAGTTTACCTTCCACCCAGCTATGGATATCTTCGGCGTCGCTTTCAAgg                         >  1:2839032/1‑69 (MQ=255)

                                   aaCTGACCCACTTTGTCGATCAGTTTACCTTCCACCCAGCTATGGATATCTTCGGCGTCGCTTTCAAgg                         >  1:2534929/1‑69 (MQ=255)

                                   aaCTGACCCACTTTGTCGATCAGTTTACCTTCCACCCAGCTATGGATATCTTCGGCGTCGCTTTCAAgg                         >  1:683987/1‑69 (MQ=255)

                                   aaCTGACCCACTTTGTCGATCAGTTTACCTTCCACCCAGCTATGGATATCTTCGGCGTCGCTTTCAAgg                         >  1:2854709/1‑69 (MQ=255)

                                   aaCTGACCCACTTTGTCGATCAGTTTACCTTCCACCCAGCTATGGATATCTTCGGCGTCGCTTTCAAgg                         >  1:478748/1‑69 (MQ=255)

                                   aaCTGACCCACTTTGTCGATCAGTTTACCTTCCACCCAGCTATGGATATCTTCGGCGTCGCTTTCAAgg                         >  1:2451151/1‑69 (MQ=255)

                                   aaCTGACCCACTTTGTCGATCAGTTTACCTTCCACCCAGCTATGGATATCTTCGGCGTCGCTTTCAAgg                         >  1:1327878/1‑69 (MQ=255)

                                   aaCTGACCCACTTTGTCGATCAGTTTACCTTCCACCCAGCTATGGATATCTTCGGCGTCGCTTTCAAgg                         >  1:2156983/1‑69 (MQ=255)

                                   aaCTGACCCACTTTGTCGATCAGTTTACCTTCCACCCAGCTATGGATATCTTCGGCGTCGCTTTCAAgg                         >  1:718311/1‑69 (MQ=255)

                                   aaCTGACCCACTTTGTCGATCAGTTTACCTTCCACCCAGCTATGGATATCTTCGGCGTCGCTTTCAAgg                         >  1:824457/1‑69 (MQ=255)

                                   aaCTGACCCACTTTGTCGATCAGTTTACCTTCCACCCAGCTATGGATATCTTCGGCGTCGCTTTCAAgg                         >  1:1838994/1‑69 (MQ=255)

                                   aaCTGACCCACTTTGTCGATCAGTTTACCTTCCACCCAGCTATGGATATCTTCGGCGTCGCTTTCAAgg                         >  1:1802486/1‑69 (MQ=255)

                                   aaCTGACCCACTTTGTCGATCAGTTTACCTTCCACCCAGCTATGGATATCTTCGGCGTCGCTTTCAAgg                         >  1:1176943/1‑69 (MQ=255)

                                   aaCTGACCCACTTTGTCGATCAGTTTACCTTCCACCCAGCTATGGATATCTTCGGCGTCGCTTTCAAgg                         >  1:3410410/1‑69 (MQ=255)

                                   aaCTGACCCACTTTGTCGATCAGTTTACCTTCCACACAGCTATGGATATCTTCGGCGTCGCTTTc                             >  1:2336420/1‑65 (MQ=255)

                                             cTTTGTCGATCAGTTTGCCTTCCACCCAGCTATGGATATCTTCGGCGTCGCTTTCAAGGATTTGTtgtg               <  1:1871683/69‑1 (MQ=255)

                                             cTTTGTCGATCAGTTTGCCTTCCACCCAGCTATGGATATCTTCGGCGTCGCTTTCAAGGATTTGTtgtg               <  1:2794791/69‑1 (MQ=255)

                                               ttGTCGATCAGTTTGCCTTCCACCCAGCTATGGATATCTTCGGCGTCGCTTTCAAGGAtt                      >  1:1472158/1‑60 (MQ=255)

                                               ttGTCGATCAGTTTGCCTTCCACCCAGCTATGGATATCTTCGGCGTCGCTTTCAAGGATTTGTTGTGGc             >  1:3155626/1‑69 (MQ=255)

                                               ttGTCGATCAGTTTGCCTTCCACCCAGCTATGGATATCTTCGGCGTCGCTTTCAAGGATTTGTTGTGGc             >  1:3298842/1‑69 (MQ=255)

                                               ttGTCGATCAGTTTGCCTTCCACCCAGCTATGGATATCTTCGGCGTCGCTTTCAAGGATTTGTTGTGGc             >  1:1691510/1‑69 (MQ=255)

                                               ttGTCGATCAGTTTGCCTTCCACCCAGCTATGGATATCTTCGGCGTCGCTTTCAAGGATTTGTTGTGGc             >  1:633874/1‑69 (MQ=255)

                                               ttGTCGATCAGTTTGCCTTCCACCCAGCTATGGATATCTTCGGCGTCGCTTTCAAGGATTTGTTGTGGc             >  1:1258832/1‑69 (MQ=255)

                                               ttGTCGATCAGTTTGCCTTCCACCCAGCTATGGATATCTTCGGCGTCGCTTTCAAGGATTTGTTGTGGc             >  1:2280525/1‑69 (MQ=255)

                                               ttGTCGATCAGTTTGCCTTCCACCCAGCTATGGATATCTTCGGCGTCGCTTTCAAGGATTTGTTGTGGc             >  1:1412229/1‑69 (MQ=255)

                                               ttGTCGATCAGTTTGCCTTCCACCCAGCTATGGATATCTTCGGCGTCGCTTTCAAGGATTTGTTGTGGc             >  1:1574891/1‑69 (MQ=255)

                                               ttGTCGATCAGTTTGCCTTCCACCCAGCTATGGATATCTTCGGCGTCGCTTTCAAGGATTTGTTGTGGc             >  1:851786/1‑69 (MQ=255)

                                               ttGTCGATCAGTTTGCCTTCCACCCAGCTATGGATATCTTCGGCGTCGCTTTCAAGGATTTGTTGTGGc             >  1:1138679/1‑69 (MQ=255)

                                               ttGTCGATCAGTTTGCCTTCCACCCAGCTATGGATATCTTCGGCGTCGCTTT‑AAGGATTTGTTGTGGcc            >  1:3202982/1‑69 (MQ=255)

                                               ttGTCGATCAGTTTACCTTCCACCCAGCTATGGATATCTTCGGCGTCGCTTTCAAGGATTTGTTGTGGc             >  1:733898/1‑69 (MQ=255)

                                                  tCGATCAGTTTACCTTCCACCCAGCTATGGATATCTTCGGCGTCGCTTTCAAGGATTTGTTGTGGcc            <  1:729539/67‑1 (MQ=255)

                                                  tCGATCAGTTTACCTTCCACCCAGCTATGGATATCTTCGGCGTCGCTTTCAAGGATTTGTTGTGGcc            <  1:1132133/67‑1 (MQ=255)

                                                  tCGATCAGTTTACCTTCCACCCAGCTATGGATATCTTCGGCGTCGCTTTCAAGGATTTGTTGTGGcc            <  1:3403610/67‑1 (MQ=255)

                                                  tCGATCAGTTTACCTTCCACCCAGCTATGGATATCTTCGGCGTCGCTTTCAAGGATTTGTTGTGGcc            <  1:1732274/67‑1 (MQ=255)

                                                  tCGATCAGTTTACCTTCCACCCAGCTATGGATATCTTCGGCGTCGCTTTCAAGGATTTGTTGTGGcc            <  1:1181270/67‑1 (MQ=255)

                                                  tCGATCAGTTTACCTTCCACCCAGCTATGGATATCTTCGGCGTCGCTTTCAAGGATTTGTTGTGGcc            <  1:3186665/67‑1 (MQ=255)

                                                  tCGATCAGTTTACCTTCCACCCAGCTATGGATATCTTCGGCGTCGCTTTCAAGGATTTGTTGTGGcc            <  1:296350/67‑1 (MQ=255)

                                                  tCGATCAGTTTACCTTCCACCCAGCTATGGATATCTTCGGCGTCGCTTTCAAGGATTTGTTGTGGcc            <  1:1446582/67‑1 (MQ=255)

                                                  tCGATCAGTTTACCTTCCACCCAGCTATGGATATCTTCGGCGTCGCTTTCAAGGATTTGTTGTGGcc            <  1:291049/67‑1 (MQ=255)

                                                  tCGATCAGTTTACCTTCCACCCAGCTATGGATATCTTCGGCGTCGCTTTCAAGGATTTGTTGTGGcc            <  1:2836835/67‑1 (MQ=255)

                                                  tCGATCAGTTTACCTTCCACCCAGCTATGGATATCTTCGGCGTCGCTTTCAAGGATTTGTTGTGGcc            <  1:2645796/67‑1 (MQ=255)

                                                  tCGATCAGTTTACCTTCCACCCAGCTATGGATATCTTCGGCGTCGCTTTCAAGGATTTGTTGTGGcc            <  1:2581032/67‑1 (MQ=255)

                                                  tCGATCAGTTTACCTTCCACCCAGCTATGGATATCTTCGGCGTCGCTTTCAAGGATTTGTTGTGGcc            <  1:2466190/67‑1 (MQ=255)

                                                  tCGATCAGTTTACCTTCCACCCAGCTATGGATATCTTCGGCGTCGCTTTCAACGATTTGTTGTGGcc            <  1:3195020/67‑1 (MQ=255)

                                                   cGATCAGTTTACCTTCCACCCAGCTATGGATATCTTCGGCGTCGCTTTCAAGGATTTGTTGTGGcc            <  1:381805/66‑1 (MQ=255)

                                                   cGATCAGTTTACCTTCCACCCAGCTATGGATATCTTCGGCGTCGCTTTCAAGGATTTGTTGTGGcc            <  1:3060553/66‑1 (MQ=255)

                                                       cAGTTTGCCTTCCACCCAGCTATGGATATCTTCGGCGTTGCTTTCAAGGATTTGTTGTGGCCTGGcgcg     <  1:1632703/69‑1 (MQ=255)

                                                       cAGTTTGCCTTCCACCCAGCTATGGATATCTTCGGCGTTGCTTTCAAGGATTTGTTGTGGCCTGGcgcg     <  1:1578746/69‑1 (MQ=255)

                                                       cAGTTTGCCTTCCACCCAGCTATGGATATCTTCGGCGTTGCTTTCAAGGATTTGTTGTGGCCTGGcgcg     <  1:8534/69‑1 (MQ=255)

                                                       cAGTTTGCCTTCCACCCAGCTATGGATATCTTCGGCGTTGCTTTCAAGGATTTGTTGTGGCCTGGcgcg     <  1:1777213/69‑1 (MQ=255)

                                                       cAGTTTGCCTTCCACCCAGCTATGGATATCTTCGGCGTTGCTTTCAAGGATTTGTTGTGGCCTGGcgcg     <  1:1556427/69‑1 (MQ=255)

                                                       cAGTTTGCCTTCCACCCAGCTATGGATATCTTCGGCGTTGCTTTCAAGGATTTGTTGTGGCCTGGcgcg     <  1:2131774/69‑1 (MQ=255)

                                                       cAGTTTGCCTTCCACCCAGCTATGGATATCTTCGGCGTTGCTTTCAAGGATTTGTTGTGGCCTGGcgcg     <  1:1224168/69‑1 (MQ=255)

                                                       cAGTTTGCCTTCCACCCAGCTATGGATATCTTCGGCGTTGCTTTCAAGGATTTGTTGTGGCCTGGcgcg     <  1:3259069/69‑1 (MQ=255)

                                                       cAGTTTGCCTTCCACCCAGCTATGGATATCTTCGGCGTTGCTTTCAAGGATTTGTTGTGGCCTGGcgcg     <  1:3364733/69‑1 (MQ=255)

                                                       cAGTTTGCCTTCCACCCAGCTATGGATATCTTCGGCGTCGCTTTCAAGGATTTGTTGTGGCCTGGcgcg     <  1:1917756/69‑1 (MQ=255)

                                                       cAGTTTGCCTTCCACCCAGCTATGGATATCTTCGGCGTCGCTTTCAAGGATTTGTTGTGGCCTGGcgcg     <  1:91861/69‑1 (MQ=255)

                                                        aGTTTGCCTTCCACCCAGCTATGGATATCTTCGGCGTTGCTTTCAAGGATTTGTTGTGGCCTGGcgcg     <  1:1154265/68‑1 (MQ=255)

                                                         gTTTGCCTTCCACCCAGCTATGGATATCTTCGGCGTTGCTTTCAAGGATTTGTTGTGGCCTGGcgcg     <  1:2961353/67‑1 (MQ=255)

                                                          tttGCCTTCCACCCAGCTATGGATATCTTCGGCGTCGCTTTCAAGGATTTGTTGTGGCCTGGCGCGAAc  <  1:3344266/69‑1 (MQ=255)

CATTACGGCTACGCCCGGTATGCAGCTTTTTGCCTAACTGGCCCACTTTGTCGATCAGTTTGCCTTCCACCCAGCTATGGATATCTTCGGCGTCGCTTTCAAGGATTTGTTGTGGCCTGGCGCGAAC  >  minE/2285207‑2285333

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`