Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,286,511 C→A 53.9% G292G (GGG→GGT)  argC ← N‑acetyl‑gamma‑glutamylphosphate reductase, NAD(P)‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,286,5110CA53.9% ‑4.0 / 117.3 100G292G (GGG→GGT) argCN‑acetyl‑gamma‑glutamylphosphate reductase, NAD(P)‑binding
Reads supporting (aligned to +/- strand):  ref base C (17/29);  new base A (20/34);  total (37/63)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.21e-01

Only 100 of 105 total aligned reads displayed.

GCGCCTTTCAGTAAGTTGTCTTCGGTCGCCACAATAATCAGATGCTCGCCCTGAACGGCAAACCCGATATCGCAAAATGGCAGCCCAACGACATTTTTCAGCGCCGGAACGCCTTTGTCA  >  minE/2286449‑2286568
                                                              |                                                         
gcgcCTTTCAGTAAGTTGTCTTCGGTCGCCACAATAATCAGATGCTCGCCCTGAACGGCAAACCCGata                                                     <  1:2689409/69‑1 (MQ=255)
gcgcCTTTCAGTAAGTTGTCTTCGGTCGCCACAATAATCAGATGCTCGCCCTGAACGGCAAACCCGata                                                     <  1:1661364/69‑1 (MQ=255)
gcgcCTTTCAGTAAGTTGTCTTCGGTCGCCACAATAATCAGATGCTCGCCCTGAACGGCAAACCCGata                                                     <  1:2698326/69‑1 (MQ=255)
gcgcCTTTCAGTAAGTTGTCTTCGGTCGCCACAATAATCAGATGCTCGCCCTGAACGGCAAACCCGata                                                     <  1:1385001/69‑1 (MQ=255)
gcgcCTTTCAGTAAGTTGTCTTCGGTCGCCACAATAATCAGATGCTCGCCCTGAACGGCAAACCCGata                                                     <  1:3129886/69‑1 (MQ=255)
gcgcCTTTCAGTAAGTTGTCTTCGGTCGCCACAATAATCAGATGCTCGCCCTGAACGGCAAACCCGata                                                     <  1:3202928/69‑1 (MQ=255)
gcgcCTTTCAGTAAGTTGTCTTCGGTCGCCACAATAATCAGATGCTCGCCCTGAACGGCAAACCCGata                                                     <  1:1268294/69‑1 (MQ=255)
gcgcCTTTCAGTAAGTTGTCTTCGGTCGCCACAATAATCAGATGCTCGCCCTGAACGGCAAACCCGata                                                     <  1:1834905/69‑1 (MQ=255)
gcgcCTTTCAGTAAGTTGTCTTCGGTCGCCACAATAATCAGATGCTCGCCCTGAACGACAAACCCGata                                                     <  1:1427086/69‑1 (MQ=255)
 cgcCTTTCAGTAAGTTGTCTTCGGTCGCCACAATAATCAGATGCTCGCCCTGAACGGCAAACCCGatat                                                    >  1:2017210/1‑69 (MQ=255)
 cgcCTTTCAGTAAGTTGTCTTCGGTCGCCACAATAATCAGATGCTCGCCCTGAACGGCAAACCCGatat                                                    >  1:789291/1‑69 (MQ=255)
 cgcCTTTCAGTAAGTTGTCTTCGGTCGCCACAATAATCAGATGCTCGCCCTGAACGGCAAACCCGatat                                                    >  1:3133707/1‑69 (MQ=255)
 cgcCTTTCAGTAAGTTGTCTTCGGTCGCCACAATAATCAGATGCTCGCCCTGAACGGCAAACCCGatat                                                    >  1:1044436/1‑69 (MQ=255)
 cgcCTTTCAGTAAGTTGTCTTCGGTCGCCACAATAATCAGATGCTCGCCCTGAACGGCAAACCCGata                                                     <  1:2224964/68‑1 (MQ=255)
          gTAAGTTGTCTTCGGTCGCCACAATAATCAGATGCTCGCCCTGAACGTCAAAACCGATATCGCAGAAt                                            <  1:3064846/68‑1 (MQ=255)
          gTAAGTTGTCTTCGGTCGCCACAATAATCAGATGCTCGCCCTGAACGGCAAAACCGATATCGCAGAAt                                            <  1:2581753/68‑1 (MQ=255)
          gTAAGTTGTCTTCGGTCGCCACAATAATCAGATGCTCGCCCTGAACGGCAAAACCGATATCGCAGAAt                                            <  1:1473641/68‑1 (MQ=255)
          gTAAGTTGTCTTCGGTCGCCACAATAATCAGATGCTCGCCCTGAACGGCAAAACCGATATCGCAGAAt                                            <  1:2025188/68‑1 (MQ=255)
          gTAAGTTGTCTTCGGTCGCCACAATAATCAGATGCTCGCCCTGAACGGCAAAACCGATATCGCAGAAt                                            <  1:221661/68‑1 (MQ=255)
          gTAAGTTGTCTTCGGTCGCCACAATAATCAGATGCTCGCCCTGAACGGCAAAACCGATATCGCAGAAt                                            <  1:2069389/68‑1 (MQ=255)
          gTAAGTTGTCTTCGGTCGCCACAATAATCAGATGCTCGCCCTGAACGGCAAAACCGATATCGCAGAAt                                            <  1:2860365/68‑1 (MQ=255)
          gTAAGTTGTCTTCGGTCGCCACAATAATCAGATGCTCGCCCTGAACGGCAAAACCGATATCGCAGAAt                                            <  1:2994048/68‑1 (MQ=255)
          gTAAGTTGTCTTCGGTCGCCACAATAATCAGATGCTCGCCCTGAACGGCAAAACCGATATCGCAGAAt                                            <  1:3234566/68‑1 (MQ=255)
          gTAAGTTGTCTTCGGTCGCCACAATAATCAGATGCTCGCCCTGAACGGCAAAACCGATATCGCAGAAt                                            <  1:3250148/68‑1 (MQ=255)
          gTAAGTTGTCTTCGGTCGCCACAATAATCAGATGCTCGCCCTGAACGGCAAAACCGATATCGCAGAAt                                            <  1:3372/68‑1 (MQ=255)
          gTAAGTTGTCTTCGGTCGCCACAATAATCAGATGCTCGCCCTGAACGGCAAAACCGATATCGCAGAAt                                            <  1:515043/68‑1 (MQ=255)
          gTAAGTTGTCTTCGGTCGCCACAATAATCAGATGCTCGCCCTGAACGGCAAAACCGATATCGCAGAAt                                            <  1:1099173/68‑1 (MQ=255)
          gTAAGTTGTCTTCGGTCGCCACAATAATCAGATGCTCGCCCTGAACGGCAAAACCGATATCGCAGAAt                                            <  1:88058/68‑1 (MQ=255)
             aGTTGTCTTCGGTCGCCACAATAATCAGATGCTCGCCCTGAACGGCAAAACCGATATCGCag                                               <  1:2986608/62‑2 (MQ=255)
             aGTTGTCTTCGGTCGCCACAATAATCAGATGCTCGCCCTGAACGGCAAAACCGATATCGCag                                               <  1:3013323/62‑2 (MQ=255)
             aGTTGTCTTCGGTCGCCACAATAATCAGATGCTCGCCCTGAACGGCAAAACCGATATCGCag                                               <  1:2783876/62‑2 (MQ=255)
             aGTTGTCTTCGGTCGCCACAATAATCAGATGCTCGCCCTGAACGGCAAAACCGATATCGCag                                               <  1:899795/62‑2 (MQ=255)
             aGTTGTCTTCGGTCGCCACAATAATCAGATGCTCGCCCTGAACGGCAAAACCGATATCGCag                                               <  1:2516505/62‑2 (MQ=255)
             aGTTGTCTTCGGTCGCCACAATAATCAGATGCTCGCCCTGAACGGCAAAACCGATATCGCag                                               <  1:2306320/62‑2 (MQ=255)
             aGTTGTCTTCGGTCGCCACAATAATCAGATGCTCGCCCTGAACGGCAAAACCGATATCGCag                                               <  1:41961/62‑2 (MQ=255)
             aGTTGTCTTCGGTCGCCACAATAATCAGATGCTCGCCCTGAACGGCAAAACCGATATCGCag                                               <  1:877757/62‑2 (MQ=255)
             aGTTGTCTTCGGTCGCCACAATAATCAGATGCTCGCCCTGAACGGCAAAACCGATATCGCag                                               <  1:2184346/62‑2 (MQ=255)
             aGTTGTCTTCGGTCGCCACAATAATCAGATGCTCGCCCTGAACGGCAAAACCGATATCGCag                                               <  1:1820678/62‑2 (MQ=255)
             aGTTGTCTTCGGTCGCCACAATAATCAGATGCTCGCCCTGAACGGCAAAACCGATATCGCag                                               <  1:2051871/62‑2 (MQ=255)
             aGTTGTCTTCGGTCGCCACAATAATCAGATGCTCGCCCTGAACGGCAAAACCGATATCGCag                                               <  1:196683/62‑2 (MQ=255)
             aGTTGTCTTCGGTCGCCACAATAATCAGATGCTCGCCCTGAACGGCAAAACCGATATCGCag                                               <  1:1211523/62‑2 (MQ=255)
             aGTTGTCTTCGGTCGCCACAATAATCAGATGCTCGCCCTGAACGGCAAAACCGATATCGCag                                               <  1:17903/62‑2 (MQ=255)
             aGTTGTCTTCGGTCGCCACAATAATCAGATGCTCGCCCTGAACGGCAAAACCGATATCGCag                                               <  1:1840634/62‑2 (MQ=255)
             aGTTGTCTTCGGTCGCCACAATAATCAGATGCTCGCCCTGAACGGCAAAACCGATATCGCag                                               <  1:1415102/62‑2 (MQ=255)
             aGTTGTCTTCGGGCGCCACAATAATCAGATGCTCGCCCTGAACGGCAAAACCGATATCGCag                                               <  1:3379558/62‑2 (MQ=255)
              gTTGTCTTCGGTCGCCACAATAATCAGATGCTCGCCCTGAACGGCAAAACCGATATCGCag                                               <  1:1816332/61‑2 (MQ=255)
               ttGTCTTCGGTCGCCACAATAATCAGATGCTCGCCCTGAACGGCAAACCCGATATCGCAAAATGGCAg                                       <  1:2988079/68‑1 (MQ=255)
               ttGTCTTCGGTCGCCACAATAATCAGATGCTCGCCCTGAACGGCAAACCCGATATCGCAAAATGGCAg                                       <  1:1957426/68‑1 (MQ=255)
               ttGTCTTCGGTCGCCACAATAATCAGATGCTCGCCCTGAACGGCAAACCCGATATCGCAAAATGGCAg                                       <  1:2594120/68‑1 (MQ=255)
               ttGTCTTCGGTCGCCACAATAATCAGATGCTCGCCCTGAACGGCAAACCCGATATCGCAAAATGGCAg                                       <  1:138080/68‑1 (MQ=255)
               ttGTCTTCGGTCGCCACAATAATCAGATGCTCGCCCTGAACGGCAAACCCGATATCGCAAAATGGCAg                                       <  1:2706713/68‑1 (MQ=255)
               ttGTCTTCGGTCGCCACAATAATCAGATGCTCGCCCTGAACGGCAAACCCGATATCGCAAAATGGCAg                                       <  1:411768/68‑1 (MQ=255)
                tGTCTTCGGTCGCCACAATAATCAGATGCTCGCCCTGAACGGCAAACCCGATATCGCAAAATGGCAGc                                      >  1:797343/1‑68 (MQ=255)
                              acaATAATCAGATGCTCGCCCTGAACGGCAAACCCGATATCGCAAAATGGCa                                        >  1:62307/1‑52 (MQ=255)
                              acaATAATCAGATGCTCGCCCTGAACGGCAAACCCGATATCGCAAAATGGCAGCCCAACGACATTTTTc                       >  1:656735/1‑69 (MQ=255)
                              acaATAATCAGATGCTCGCCCTGAACGGCAAACCCGATATCGCAAAATGGCAGCCCAACGACATTTTTc                       <  1:1140733/69‑1 (MQ=255)
                               caATAATCAGATGCTCGCCCTGAACGGCAAACCCGATATCGCAAAATGGCAGCCCAACGACATTTTTc                       <  1:3405311/68‑1 (MQ=255)
                                 ataatCAGATGCTCGCCCTGAACGGCAAACCCGATATCGCAAAATGGCAGCCCAACGACATTTTTCAgc                    >  1:861270/1‑69 (MQ=255)
                                 ataatCAGATGCTCGCCCTGAACGGCAAAACCGATATCGCAGAATGGCAGTCCAACGACATTTTTCAgc                    >  1:1133013/1‑69 (MQ=255)
                                 ataatCAGATGCTCGCCCTGAACGGCAAAACCGATATCGCAGAATGGCAGTCCAACGACATTTTTCAgc                    >  1:2209457/1‑69 (MQ=255)
                                 ataatCAGATGCTCGCCCTGAACGGCAAAACCGATATCGCAGAATGGCAGTCCAACGACATTTTTCAgc                    >  1:405078/1‑69 (MQ=255)
                                 ataatCAGATGCTCGCCCTGAACGGCAAAACCGATATCGCAGAATGGCAGTCCAACGACATTTTTCAgc                    >  1:3427953/1‑69 (MQ=255)
                                 ataatCAGATGCTCGCCCTGAACGGCAAAACCGATATCGCAGAATGGCAGTCCAACGACATTTTTCAgc                    >  1:2166704/1‑69 (MQ=255)
                                 ataatCAGATGCTCGCCCTGAACGGCAAAACCGATATCGCAGAATGGCAGTCCAACGACATTTTTCAgc                    >  1:1190034/1‑69 (MQ=255)
                                 ataatCAGATGCTCGCCCTGAACGGCAAAACCGATATCGCAGAATGGCAGTCCAACGACATTTTTCAgc                    >  1:697619/1‑69 (MQ=255)
                                 ataatCAGATGCTCGCCCTGAACGGCAAAACCGATATCGCAGAATGGCAGTCCAACGACATTTTTCAgc                    >  1:2638402/1‑69 (MQ=255)
                                 ataatCAGATGCTCGCCCTGAACGGCAAAACCGATATCGCAGAATGGCAGTCCAACGACATTTTTCAgc                    >  1:3157968/1‑69 (MQ=255)
                                 ataatCAGATGCTCGCCCTGAACGGCAAAACCGATATCGCAGAATGGCAGTCCAACGACATTTTTCAgc                    >  1:2285931/1‑69 (MQ=255)
                                 ataatCAGATGCTCGCCCTGAACGGCAAAACCGATATCGCAGAATGGCAGTCCAACGACATTTTTCAgc                    >  1:1586983/1‑69 (MQ=255)
                                 ataatCAGATGCTCGCCCTGAACGGCAAAACCGATATCGCAGAATGGCAGTCCAACGACATTTTTCAgc                    >  1:1645774/1‑69 (MQ=255)
                                 ataatCAGATGCTCGCCCTGAACGGCAAAACCGATATCGCAGAATGGCAGTCCAACGACATTTTTCAgc                    >  1:1943795/1‑69 (MQ=255)
                                 ataatCAGATGCTCGCCCTGAACGGCAAAACCGATATCGCAGAATGGCAGTCCAACGACATTTTTCAgc                    >  1:875973/1‑69 (MQ=255)
                                 ataatCAGATGCTCGCCCTGAACGGCAAAACCGATATCGCAGAATGGCAGTCCAACGACATTTTTCAgc                    >  1:642836/1‑69 (MQ=255)
                                 ataatCAGATGCTCGCCCTGAACGGCAAAACCGATATCGCAGAATGGCAGTCCAACGACATTTTTCAgc                    >  1:271/1‑69 (MQ=255)
                                 ataatCAGATGCTCGCCCTGAACGGCAAAACCGATATCGCAGAATGGCAGTCCAACGACATTTTTCAg                     >  1:1182129/1‑68 (MQ=255)
                                 ataatCAGATGCTCGCCCTGAACGGCAAAACCGATATCGCAGAATCGCAGTCCAACGACATTTTTCAgc                    >  1:3111834/1‑69 (MQ=255)
                                 ataatCAGATGCTAGCCCTGAACGGCAAAACCGATATCGCAGAATGGCAGTCCAACGACATTTTTCAgc                    >  1:3258147/1‑69 (MQ=255)
                                      cAGATGCTCGCCCTGAACGGCAAACCCGATATCGCAAAATGGCAGCCCAACGACATTTTTCAGCGCCgg               <  1:2344992/69‑1 (MQ=255)
                                      cAGATGCTCGCCCTGAACGGCAAACCCGATATCGCAAAATGGCAGCCCAACGACATTTTTCAGCGCCgg               <  1:724412/69‑1 (MQ=255)
                                      cAGATGCTCGCCCTGAACGGCAAACCCGATATCGCAAAATGGCAGCCCAACGACATTTTTCAGCGCCgg               <  1:2270271/69‑1 (MQ=255)
                                      cAGATGCTCGCCCTGAACGGCAAACCCGATATCGCAAAATGGCAGCCCAACGACATTTTTCAGCGCCgg               <  1:509765/69‑1 (MQ=255)
                                      cAGATGCTCGCCCTGAACGGCAAACCCGATATCGCAAAATGGCAGCCCAACGACATTTTTCAGCGCCgg               <  1:1678027/69‑1 (MQ=255)
                                        gATGCTCGCCCTGAACGGCAAAACCGATATCGCag                                               <  1:3191858/35‑2 (MQ=38)
                                                  cTGAACGGCAAACCCGATATCGCAAAATGGCAGCGCAACGACATTTTTCAGCGCCgg               <  1:1060702/57‑1 (MQ=255)
                                                  cTGAACGGCAAACCCGATATCGCAAAATGGCAGCCCAACGACa                             >  1:2862558/1‑43 (MQ=255)
                                                  cTGAACGGCAAACCCGATATCGCAAAATGGCAGCCCAACGACATTTTTCAGCGCCGGAACGCCTTTGt    >  1:1126107/1‑68 (MQ=255)
                                                  cTGAACGGCAAACCCGATATCGCAAAATGGCAGCCCAACGACATTTTTCAGCGCCGGAACGCCTTTGt    >  1:1000464/1‑68 (MQ=255)
                                                  cTGAACGGCAAACCCGATATCGCAAAATGGCAGCCCAACGACATTTTTCAGCGCCGGAACGCCTTTGt    >  1:578538/1‑68 (MQ=255)
                                                  cTGAACGGCAAACCCGATATCGCAAAATGGCAGCCCAACGACATTTTTCAGCGCCGGAACGCCTTTGt    >  1:461517/1‑68 (MQ=255)
                                                  cTGAACGGCAAACCCGATATCGCAAAATGGCAGCCCAACGACATTTTTCAGCGCCGGAACGCCTTTGt    >  1:1294148/1‑68 (MQ=255)
                                                  cTGAACGGCAAACCCGATATCGCAAAATGGCAGCCCAACGACATTTTTCAGCGCCGGAACGCCTTTGt    >  1:299323/1‑68 (MQ=255)
                                                  cTGAACGGCAAACCCGATATCGCAAAATGGCAGCCCAACGACATTTTTCAGCGCCGGAACGCCTTTGt    >  1:1693389/1‑68 (MQ=255)
                                                  cTGAACGGCAAACCCGATATCGCAAAATGGCAGCCCAACGACATTTTTCAGCGCCGGAACGCCTTTGt    >  1:2263016/1‑68 (MQ=255)
                                                   tGAACGGCAAACCCGATATCGCAAAATGGCAGCCCAACGACATTTTTCAGCGCCGGAACGCCTTTGTCa  <  1:683017/69‑1 (MQ=255)
                                                   tGAACGGCAAACCCGATATCGCAAAATGGCAGCCCAACGACATTTTTCAGCGCCGGAACGCCTTTGTCa  <  1:2401053/69‑1 (MQ=255)
                                                              cccGATATCGCAAAATGGCAGCCCAACGACATTTTTCAGCGCCGGAACGCCTTTGTCa  <  1:1258048/58‑1 (MQ=255)
                                                              cccGATATCGCAAAATGGCAGCCCAACGACATTTTTCAGCGCCGGAACGCCTTTGTCa  <  1:705294/58‑1 (MQ=255)
                                                              cccGATATCGCAAAATGGCAGCCCAACGACATTTTTCAGCGCCGGAACGCCTTTGTCa  <  1:1271912/58‑1 (MQ=255)
                                                              cccGATATCGCAAAATGGCAGCCCAACGACATTTTTCAGCGCCGGAACGCCTTTGTCa  <  1:281012/58‑1 (MQ=255)
                                                              cccGATATCGCAAAATGGCAGCCCAACGACATTTTTCAGCGCCGGAACGCCTTTGTCa  <  1:2605288/58‑1 (MQ=255)
                                                              |                                                         
GCGCCTTTCAGTAAGTTGTCTTCGGTCGCCACAATAATCAGATGCTCGCCCTGAACGGCAAACCCGATATCGCAAAATGGCAGCCCAACGACATTTTTCAGCGCCGGAACGCCTTTGTCA  >  minE/2286449‑2286568

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑