Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | minE | 2,286,523 | A→G | 47.9% | F288F (TTT→TTC) | argC ← | N‑acetyl‑gamma‑glutamylphosphate reductase, NAD(P)‑binding |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | minE | 2,286,523 | 0 | A | G | 47.9% | ‑1.6 / 63.0 | 71 | F288F (TTT→TTC) | argC | N‑acetyl‑gamma‑glutamylphosphate reductase, NAD(P)‑binding |
Reads supporting (aligned to +/- strand): ref base A (13/24); new base G (20/14); total (33/38) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 5.86e-02 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01 |
GTAAGTTGTCTTCGGTCGCCACAATAATCAGATGCTCGCCCTGAACGGCAAACCCGATATCGCAAAATGGCAGCCCAACGACATTTTTCAGCGCCGGAACGCCTTTGTCA > minE/2286459‑2286568 | gTAAGTTGTCTTCGGTCGCCACAATAATCAGATGCTCGCCCTGAACGTCAAAACCGATATCGCAGAAt < 1:3064846/68‑1 (MQ=255) gTAAGTTGTCTTCGGTCGCCACAATAATCAGATGCTCGCCCTGAACGGCAAAACCGATATCGCAGAAt < 1:88058/68‑1 (MQ=255) gTAAGTTGTCTTCGGTCGCCACAATAATCAGATGCTCGCCCTGAACGGCAAAACCGATATCGCAGAAt < 1:2025188/68‑1 (MQ=255) gTAAGTTGTCTTCGGTCGCCACAATAATCAGATGCTCGCCCTGAACGGCAAAACCGATATCGCAGAAt < 1:2069389/68‑1 (MQ=255) gTAAGTTGTCTTCGGTCGCCACAATAATCAGATGCTCGCCCTGAACGGCAAAACCGATATCGCAGAAt < 1:3372/68‑1 (MQ=255) gTAAGTTGTCTTCGGTCGCCACAATAATCAGATGCTCGCCCTGAACGGCAAAACCGATATCGCAGAAt < 1:515043/68‑1 (MQ=255) gTAAGTTGTCTTCGGTCGCCACAATAATCAGATGCTCGCCCTGAACGGCAAAACCGATATCGCAGAAt < 1:1473641/68‑1 (MQ=255) gTAAGTTGTCTTCGGTCGCCACAATAATCAGATGCTCGCCCTGAACGGCAAAACCGATATCGCAGAAt < 1:221661/68‑1 (MQ=255) gTAAGTTGTCTTCGGTCGCCACAATAATCAGATGCTCGCCCTGAACGGCAAAACCGATATCGCAGAAt < 1:3250148/68‑1 (MQ=255) gTAAGTTGTCTTCGGTCGCCACAATAATCAGATGCTCGCCCTGAACGGCAAAACCGATATCGCAGAAt < 1:3234566/68‑1 (MQ=255) gTAAGTTGTCTTCGGTCGCCACAATAATCAGATGCTCGCCCTGAACGGCAAAACCGATATCGCAGAAt < 1:2994048/68‑1 (MQ=255) gTAAGTTGTCTTCGGTCGCCACAATAATCAGATGCTCGCCCTGAACGGCAAAACCGATATCGCAGAAt < 1:2581753/68‑1 (MQ=255) gTAAGTTGTCTTCGGTCGCCACAATAATCAGATGCTCGCCCTGAACGGCAAAACCGATATCGCAGAAt < 1:2860365/68‑1 (MQ=255) gTAAGTTGTCTTCGGTCGCCACAATAATCAGATGCTCGCCCTGAACGGCAAAACCGATATCGCAGAAt < 1:1099173/68‑1 (MQ=255) ttGTCTTCGGTCGCCACAATAATCAGATGCTCGCCCTGAACGGCAAACCCGATATCGCAAAATGGCAg < 1:2594120/68‑1 (MQ=255) ttGTCTTCGGTCGCCACAATAATCAGATGCTCGCCCTGAACGGCAAACCCGATATCGCAAAATGGCAg < 1:2988079/68‑1 (MQ=255) ttGTCTTCGGTCGCCACAATAATCAGATGCTCGCCCTGAACGGCAAACCCGATATCGCAAAATGGCAg < 1:2706713/68‑1 (MQ=255) ttGTCTTCGGTCGCCACAATAATCAGATGCTCGCCCTGAACGGCAAACCCGATATCGCAAAATGGCAg < 1:1957426/68‑1 (MQ=255) ttGTCTTCGGTCGCCACAATAATCAGATGCTCGCCCTGAACGGCAAACCCGATATCGCAAAATGGCAg < 1:411768/68‑1 (MQ=255) ttGTCTTCGGTCGCCACAATAATCAGATGCTCGCCCTGAACGGCAAACCCGATATCGCAAAATGGCAg < 1:162422/68‑1 (MQ=255) ttGTCTTCGGTCGCCACAATAATCAGATGCTCGCCCTGAACGGCAAACCCGATATCGCAAAATGGCAg < 1:138080/68‑1 (MQ=255) tGTCTTCGGTCGCCACAATAATCAGATGCTCGCCCTGAACGGCAAACCCGATATCGCAAAATGGCAGc > 1:797343/1‑68 (MQ=255) acaATAATCAGATGCTCGCCCTGAACGGCAAACCCGATATCGCAAAATGGCa > 1:62307/1‑52 (MQ=255) acaATAATCAGATGCTCGCCCTGAACGGCAAACCCGATATCGCAAAATGGCAGCCCAACGACATTTTTc < 1:1140733/69‑1 (MQ=255) acaATAATCAGATGCTCGCCCTGAACGGCAAACCCGATATCGCAAAATGGCAGCCCAACGACATTTTTc > 1:656735/1‑69 (MQ=255) caATAATCAGATGCTCGCCCTGAACGGCAAACCCGATATCGCAAAATGGCAGCCCAACGACATTTTTc < 1:3405311/68‑1 (MQ=255) ataatCAGATGCTCGCCCTGAACGGCAAACCCGATATCGCAAAATGGCAGCCCAACGACATTTTTCAgc > 1:861270/1‑69 (MQ=255) ataatCAGATGCTCGCCCTGAACGGCAAAACCGATATCGCAGAATGGCAGTCCAACGACATTTTTCAgc > 1:642836/1‑69 (MQ=255) ataatCAGATGCTCGCCCTGAACGGCAAAACCGATATCGCAGAATGGCAGTCCAACGACATTTTTCAgc > 1:271/1‑69 (MQ=255) ataatCAGATGCTCGCCCTGAACGGCAAAACCGATATCGCAGAATGGCAGTCCAACGACATTTTTCAgc > 1:875973/1‑69 (MQ=255) ataatCAGATGCTCGCCCTGAACGGCAAAACCGATATCGCAGAATGGCAGTCCAACGACATTTTTCAgc > 1:697619/1‑69 (MQ=255) ataatCAGATGCTCGCCCTGAACGGCAAAACCGATATCGCAGAATGGCAGTCCAACGACATTTTTCAgc > 1:3157968/1‑69 (MQ=255) ataatCAGATGCTCGCCCTGAACGGCAAAACCGATATCGCAGAATGGCAGTCCAACGACATTTTTCAgc > 1:3427953/1‑69 (MQ=255) ataatCAGATGCTCGCCCTGAACGGCAAAACCGATATCGCAGAATGGCAGTCCAACGACATTTTTCAgc > 1:408951/1‑69 (MQ=255) ataatCAGATGCTCGCCCTGAACGGCAAAACCGATATCGCAGAATGGCAGTCCAACGACATTTTTCAgc > 1:405078/1‑69 (MQ=255) ataatCAGATGCTCGCCCTGAACGGCAAAACCGATATCGCAGAATGGCAGTCCAACGACATTTTTCAgc > 1:1133013/1‑69 (MQ=255) ataatCAGATGCTCGCCCTGAACGGCAAAACCGATATCGCAGAATGGCAGTCCAACGACATTTTTCAgc > 1:2638402/1‑69 (MQ=255) ataatCAGATGCTCGCCCTGAACGGCAAAACCGATATCGCAGAATGGCAGTCCAACGACATTTTTCAgc > 1:1645774/1‑69 (MQ=255) ataatCAGATGCTCGCCCTGAACGGCAAAACCGATATCGCAGAATGGCAGTCCAACGACATTTTTCAgc > 1:1943795/1‑69 (MQ=255) ataatCAGATGCTCGCCCTGAACGGCAAAACCGATATCGCAGAATGGCAGTCCAACGACATTTTTCAgc > 1:1190034/1‑69 (MQ=255) ataatCAGATGCTCGCCCTGAACGGCAAAACCGATATCGCAGAATGGCAGTCCAACGACATTTTTCAgc > 1:1586983/1‑69 (MQ=255) ataatCAGATGCTCGCCCTGAACGGCAAAACCGATATCGCAGAATGGCAGTCCAACGACATTTTTCAgc > 1:2166704/1‑69 (MQ=255) ataatCAGATGCTCGCCCTGAACGGCAAAACCGATATCGCAGAATGGCAGTCCAACGACATTTTTCAgc > 1:2209457/1‑69 (MQ=255) ataatCAGATGCTCGCCCTGAACGGCAAAACCGATATCGCAGAATGGCAGTCCAACGACATTTTTCAgc > 1:2285931/1‑69 (MQ=255) ataatCAGATGCTCGCCCTGAACGGCAAAACCGATATCGCAGAATGGCAGTCCAACGACATTTTTCAg > 1:1182129/1‑68 (MQ=255) ataatCAGATGCTCGCCCTGAACGGCAAAACCGATATCGCAGAATCGCAGTCCAACGACATTTTTCAgc > 1:3111834/1‑69 (MQ=255) ataatCAGATGCTAGCCCTGAACGGCAAAACCGATATCGCAGAATGGCAGTCCAACGACATTTTTCAgc > 1:3258147/1‑69 (MQ=255) cAGATGCTCGCCCTGAACGGCAAACCCGATATCGCAAAATGGCAGCCCAACGACATTTTTCAGCGCCgg < 1:2270271/69‑1 (MQ=255) cAGATGCTCGCCCTGAACGGCAAACCCGATATCGCAAAATGGCAGCCCAACGACATTTTTCAGCGCCgg < 1:509765/69‑1 (MQ=255) cAGATGCTCGCCCTGAACGGCAAACCCGATATCGCAAAATGGCAGCCCAACGACATTTTTCAGCGCCgg < 1:2344992/69‑1 (MQ=255) cAGATGCTCGCCCTGAACGGCAAACCCGATATCGCAAAATGGCAGCCCAACGACATTTTTCAGCGCCgg < 1:724412/69‑1 (MQ=255) cAGATGCTCGCCCTGAACGGCAAACCCGATATCGCAAAATGGCAGCCCAACGACATTTTTCAGCGCCgg < 1:2884444/69‑1 (MQ=255) cAGATGCTCGCCCTGAACGGCAAACCCGATATCGCAAAATGGCAGCCCAACGACATTTTTCAGCGCCgg < 1:1678027/69‑1 (MQ=255) cTGAACGGCAAACCCGATATCGCAAAATGGCAGCGCAACGACATTTTTCAGCGCCgg < 1:1060702/57‑1 (MQ=255) cTGAACGGCAAACCCGATATCGCAAAATGGCAGCCCAACGACa > 1:2862558/1‑43 (MQ=255) cTGAACGGCAAACCCGATATCGCAAAATGGCAGCCCAACGACATTTTTCAGCGCCGGAACGCCTTTGt > 1:461517/1‑68 (MQ=255) cTGAACGGCAAACCCGATATCGCAAAATGGCAGCCCAACGACATTTTTCAGCGCCGGAACGCCTTTGt > 1:1000464/1‑68 (MQ=255) cTGAACGGCAAACCCGATATCGCAAAATGGCAGCCCAACGACATTTTTCAGCGCCGGAACGCCTTTGt > 1:1126107/1‑68 (MQ=255) cTGAACGGCAAACCCGATATCGCAAAATGGCAGCCCAACGACATTTTTCAGCGCCGGAACGCCTTTGt > 1:299323/1‑68 (MQ=255) cTGAACGGCAAACCCGATATCGCAAAATGGCAGCCCAACGACATTTTTCAGCGCCGGAACGCCTTTGt > 1:1294148/1‑68 (MQ=255) cTGAACGGCAAACCCGATATCGCAAAATGGCAGCCCAACGACATTTTTCAGCGCCGGAACGCCTTTGt > 1:578538/1‑68 (MQ=255) cTGAACGGCAAACCCGATATCGCAAAATGGCAGCCCAACGACATTTTTCAGCGCCGGAACGCCTTTGt > 1:2263016/1‑68 (MQ=255) cTGAACGGCAAACCCGATATCGCAAAATGGCAGCCCAACGACATTTTTCAGCGCCGGAACGCCTTTGt > 1:1693389/1‑68 (MQ=255) tGAACGGCAAACCCGATATCGCAAAATGGCAGCCCAACGACATTTTTCAGCGCCGGAACGCCTTTGTCa < 1:683017/69‑1 (MQ=255) tGAACGGCAAACCCGATATCGCAAAATGGCAGCCCAACGACATTTTTCAGCGCCGGAACGCCTTTGTCa < 1:2401053/69‑1 (MQ=255) cccGATATCGCAAAATGGCAGCCCAACGACATTTTTCAGCGCCGGAACGCCTTTGTCa < 1:1271912/58‑1 (MQ=255) cccGATATCGCAAAATGGCAGCCCAACGACATTTTTCAGCGCCGGAACGCCTTTGTCa < 1:1258048/58‑1 (MQ=255) cccGATATCGCAAAATGGCAGCCCAACGACATTTTTCAGCGCCGGAACGCCTTTGTCa < 1:705294/58‑1 (MQ=255) cccGATATCGCAAAATGGCAGCCCAACGACATTTTTCAGCGCCGGAACGCCTTTGTCa < 1:2605288/58‑1 (MQ=255) cccGATATCGCAAAATGGCAGCCCAACGACATTTTTCAGCGCCGGAACGCCTTTGTCa < 1:281012/58‑1 (MQ=255) gATATCGCAAAATGGCAGCACAACGACATTTTTCAGCGCCGGAACGCCTTTGTCa < 1:712846/55‑1 (MQ=255) | GTAAGTTGTCTTCGGTCGCCACAATAATCAGATGCTCGCCCTGAACGGCAAACCCGATATCGCAAAATGGCAGCCCAACGACATTTTTCAGCGCCGGAACGCCTTTGTCA > minE/2286459‑2286568 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |