Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,287,806 T→A 49.5% G89G (GGT→GGA argE → acetylornithine deacetylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,287,8060TA49.5% ‑4.6 / 120.3 99G89G (GGT→GGAargEacetylornithine deacetylase
Reads supporting (aligned to +/- strand):  ref base T (27/23);  new base A (30/19);  total (57/42)
Fisher's exact test for biased strand distribution p-value = 5.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.19e-01

TATCGGACAGGGGGCTGGCGGCTTGTTGCTGGCGGGGCATACCGATACGGTGCCATTTGATGACGGTCGCTGGACGCGCGATCCGTTTACACTGACGGAGCATGACGGCAAGCTTTACGGCTTAGGCACCGCCGA  >  minE/2287740‑2287874
                                                                  |                                                                    
tATCGGACAGGGGGCTGGCGGCTTGTTGCTGGCGGGGCATACCGATACGGTGCCATTTGATGACGGTCg                                                                    >  1:2583968/1‑69 (MQ=255)
          ggggCTGGCGGCTTGTTGCTGGCGGGGCATACCGATACGGTGCCATTTGATGACGGTCGCTGGAcgcgc                                                          >  1:3063738/1‑69 (MQ=255)
          ggggCTGGCGGCTTGTTGCTGGCGGGGCATACCGATACGGTGCCATTTGATGACGGTCGCTGGAcgcgc                                                          >  1:1302300/1‑69 (MQ=255)
          ggggCTGGCGGCTTGTTGCTGGCGGGGCATACCGATACGGTGCCATTTGATGACGGTCGCAGGAcgcgc                                                          >  1:1867268/1‑69 (MQ=255)
                   ggcTTGTTGCTGGCGGGGCATACCGATACGGTGCCATTTGATGACGGTCGCTGGACGCGCGATCCGtt                                                  <  1:106184/68‑1 (MQ=255)
                   ggcTTGTTGCTGGCGGGGCATACCGATACGGTGCCATTTGATGACGGTCGCTGGACGCGCGATCCGtt                                                  <  1:2008676/68‑1 (MQ=255)
                   ggcTTGTTGCTGGCGGGGCATACCGATACGGTGCCATTTGATGACGGTCGCTGGACGCGCGATCCGtt                                                  <  1:2033025/68‑1 (MQ=255)
                    gcTTGTTGCTGGCGGGGCATACCGATACGGTGCCATTTGATGACGGTCGCTGGACTCGCGATCCGTTTa                                                <  1:2238697/69‑1 (MQ=255)
                    gcTTGTTGCTGGCGGGGCATACCGATACGGTGCCATTTGATGACGGTCGCTGGACGCGCGATCCGTTTa                                                <  1:2992238/69‑1 (MQ=255)
                    gcTTGTTGCTGGCGGGGCATACCGATACGGTGCCATTTGATGACGGTCGCTGGACGCGCGATCCGTTTa                                                <  1:186694/69‑1 (MQ=255)
                    gcTTGTTGCTGGCGGGGCATACCGATACGGTGCCATTTGATGACGGTCGCTGGACGCGCGATCCGTTTa                                                <  1:3185652/69‑1 (MQ=255)
                    gcTTGTTGCTGGCGGGGCATACCGATACGGTGCCATTTGATGACGGTCGCTGGACGCGCGATCCGTTTa                                                <  1:642162/69‑1 (MQ=255)
                    gcTTGTTGCTGGCGGGGCATACCGACACGGTGCCATTTGATGACGGTCGCTGGACGCGCGATCCGTTTa                                                <  1:2604983/69‑1 (MQ=255)
                     cTTGTTGCTGGCGGGGCATACCGATACGGTGCCATTTGATGACGGTCGCTGGACGCGCGATCCGTTTac                                               <  1:2187425/69‑1 (MQ=255)
                     cTTGTTGCTGGCGGGGCATACCGATACGGTGCCATTTGATGACGGTCGCTGGACGCGCGATCCGTTTac                                               <  1:1404286/69‑1 (MQ=255)
                     cTTGTTGCTGGCGGGGCATACCGATACGGTGCCATTTGATGACGGTCGCTGGACGCGCGATCCGTTTac                                               <  1:296957/69‑1 (MQ=255)
                     cTTGTTGCTGGCGGGGCATACCGATACGGTGCCATTTGATGACGGTCGCTGGACGCGCGATCCGTTTac                                               <  1:2354553/69‑1 (MQ=255)
                     cTTGTTGCTGGAGGGGCATACCGATACGGTGCCATTTGATGACGGTCGCTGGACGCGCGATCCGTTTac                                               <  1:1015719/69‑1 (MQ=255)
                      ttgttgCTGGCGGGGCATACCGATACGGTGCCATTTGATGACGGTCGCTGGACGCGCGATCCGTTTac                                               <  1:631412/68‑1 (MQ=255)
                                  gggCATACCGATACGGTGCCATTTGATGACGGTCGCTGGACGCGCGATCCGTTTACACTGACGGAGCAt                                  <  1:2116588/69‑1 (MQ=255)
                                  gggCATACCGATACGGTGCCATTTGATGACGGTCGCTGGACGCGCGATCCGTTTACACTGACGGAGCAt                                  <  1:3257407/69‑1 (MQ=255)
                                      aTACCGATACGGTGCCATTTGATGACGGTCGCTGGACGCGCGATCCGTTTACACTGACGGAg                                     <  1:96267/62‑1 (MQ=255)
                                         ccGATACGGTGCCATTTGATGACGGTCGCTGGACGCGCGATCCGTTTACACTGACGGAGCATGACGGCa                           >  1:795270/1‑69 (MQ=255)
                                         ccGATACGGTGCCATTTGATGACGGTCGCTGGACGCGCGATCCGTTTACACTGACGGAGCATGACGGCa                           >  1:290533/1‑69 (MQ=255)
                                         ccGATACGGTGCCATTTGATGACGGTCGCTGGACGCGCGATCCGTTTACACTGACGGAGCATGACGGCa                           >  1:2314431/1‑69 (MQ=255)
                                         ccGATACGGTGCCATTTGATGACGGTCGCTGGACGCGCGATCCGTTTACACTGACGGAGCATGACGGCa                           >  1:282035/1‑69 (MQ=255)
                                         ccGATACGGTGCCATTTGATGACGGTCGCTGGACGCGCGATCCGTTTACACTGACGGAGCATGACGGCa                           >  1:2786214/1‑69 (MQ=255)
                                         ccGATACGGTGCCATTTGATGACGGTCGCTGGACGCGCGATCCGTTTACACTGACGGAGCATGACGGCa                           >  1:2657080/1‑69 (MQ=255)
                                         ccGATACGGTGCCATTTGATGACGGTCGCTGGACGCGCGATCCGTTTACACTGACGGAGCATGACGGCa                           >  1:770817/1‑69 (MQ=255)
                                         ccGATACGGTGCCATTTGATGACGGTCGCTGGACGCGCGATCCGTTTACACTGACGGAGCATGACGGCa                           >  1:744708/1‑69 (MQ=255)
                                         ccGATACGGTGCCATTTGATGACGGTCGCTGGACGCGCGATCCGTTTACACTGACGGAGCATGACGGCa                           >  1:3394808/1‑69 (MQ=255)
                                         ccGATACGGTGCCATTTGATGACGGTCGCTGGACGCGCGATCCGTTTACACTGACGGAGCATGACGGCa                           >  1:591909/1‑69 (MQ=255)
                                         ccGATACGGTGCCATTTGATGACGGTCGCTGGACGCGCGATCCGTTTACACTGACGGAGCATGACGGCa                           >  1:1939200/1‑69 (MQ=255)
                                         ccGATACGGTGCCATTTGATGACGGTCGCTGGACGCGCGATCCGTTTACACTGACGGAGCATGACGGCa                           >  1:389125/1‑69 (MQ=255)
                                              aCGGTGCCATTTGATGACGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCtt                      <  1:271395/69‑1 (MQ=255)
                                              aCGGTGCCATTTGATGACGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCtt                      <  1:2598903/69‑1 (MQ=255)
                                              aCGGTGCCATTTGATGACGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCtt                      <  1:710851/69‑1 (MQ=255)
                                              aCGGTGCCATTTGATGACGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCtt                      <  1:658918/69‑1 (MQ=255)
                                              aCGGTGCCATTTGATGACGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCtt                      <  1:559167/69‑1 (MQ=255)
                                              aCGGTGCCATTTGATGACGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCtt                      <  1:368109/69‑1 (MQ=255)
                                              aCGGTGCCATTTGATGACGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCtt                      <  1:3336505/69‑1 (MQ=255)
                                              aCGGTGCCATTTGATGACGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCtt                      <  1:3298319/69‑1 (MQ=255)
                                              aCGGTGCCATTTGATGACGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCtt                      <  1:967607/69‑1 (MQ=255)
                                              aCGGTGCCATTTGATGACGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCtt                      <  1:3042362/69‑1 (MQ=255)
                                              aCGGTGCCATTTGATGACGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCtt                      <  1:2995223/69‑1 (MQ=255)
                                              aCGGTGCCATTTGATGACGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCtt                      <  1:1297888/69‑1 (MQ=255)
                                              aCGGTGCCATTTGATGACGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCtt                      <  1:1935473/69‑1 (MQ=255)
                                              aCGGTGCCATTTGATGACGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCtt                      <  1:2009935/69‑1 (MQ=255)
                                              aCGGTGCCATTTGATGACGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCtt                      <  1:2162786/69‑1 (MQ=255)
                                              aCGGTGCCATTTGATGACGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCtt                      <  1:1100472/69‑1 (MQ=255)
                                              aCGGTGCCATTTGATGACGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCtt                      <  1:1210523/69‑1 (MQ=255)
                                              aCGGTGCCATTTGATGACGGACGCTGGACGCGCGATCCATTTACGTTGAAGGAGCATGACGGCAAGCtt                      <  1:2093092/69‑1 (MQ=255)
                                                ggTGCCATTTGATGACGGTCGCTGGACGCGCGATCCGTTTACACTGACGGAGCAt                                  <  1:3288302/55‑1 (MQ=255)
                                                   gCCATTTGATGACGGTCGCTGGACGCGCGATCCGTTTACACTGACGGAg                                     >  1:2776493/1‑49 (MQ=255)
                                                   gCCATTTGATGACGGTCGCTGGACGCGCGATCCGTTTACACTGACGGAg                                     >  1:2996019/1‑49 (MQ=255)
                                                   gCCATTTGATGACGGTCGCTGGACGCGCGATCCGTTTACACTGACGGAg                                     >  1:2486725/1‑49 (MQ=255)
                                                   gCCATTTGATGACGGTCGCTGGACGCGCGATCCGTTTACACTGACGGAg                                     >  1:1234201/1‑49 (MQ=255)
                                                    ccATTTGATGACGGTCGCTGGACGCGCGATCCGTTTACACTGACGGAGCATGACGGCAAGCTTTACGGc                <  1:2464681/69‑1 (MQ=255)
                                                    ccATTTGATGACGGTCGCTGGACGCGCGATCCGTTTACACTGACGGAGCATGACGGCAAGCTTTACGGc                <  1:1706786/69‑1 (MQ=255)
                                                        ttGATGACGGACGCTGGACGCGCGATCCATTTACGTTGACGGAg                                     >  1:816020/1‑44 (MQ=255)
                                                        ttGATGACGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCTTTACGGCTTa             >  1:1235976/1‑68 (MQ=255)
                                                        ttGATGACGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCTTTACGGCTTa             >  1:635958/1‑68 (MQ=255)
                                                        ttGATGACGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCTTTACGGCTTa             >  1:1049989/1‑68 (MQ=255)
                                                        ttGATGACGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCTTTACGGCTTa             >  1:1098310/1‑68 (MQ=255)
                                                        ttGATGACGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCTTTACGGCTTa             >  1:533846/1‑68 (MQ=255)
                                                        ttGATGACGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCTTTACGGCTTa             >  1:507934/1‑68 (MQ=255)
                                                        ttGATGACGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCTTTACGGCTTa             >  1:507753/1‑68 (MQ=255)
                                                        ttGATGACGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCTTTACGGCTTa             >  1:499361/1‑68 (MQ=255)
                                                        ttGATGACGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCTTTACGGCTTa             >  1:1329895/1‑68 (MQ=255)
                                                        ttGATGACGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCTTTACGGCTTa             >  1:3319377/1‑68 (MQ=255)
                                                        ttGATGACGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCTTTACGGCTTa             >  1:1022743/1‑68 (MQ=255)
                                                        ttGATGACGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCTTTACGGCTTa             >  1:1709977/1‑68 (MQ=255)
                                                        ttGATGACGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCTTTACGGCTTa             >  1:3196043/1‑68 (MQ=255)
                                                        ttGATGACGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCTTTACGGCTTa             >  1:2501953/1‑68 (MQ=255)
                                                        ttGATGACGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCTTTACGGCTTa             >  1:2476565/1‑68 (MQ=255)
                                                        ttGATGACGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCTTTACGGCTTa             >  1:1710545/1‑68 (MQ=255)
                                                        ttGATGACGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCTTTACGGCTTa             >  1:2688668/1‑68 (MQ=255)
                                                        ttGATGACGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCTTTACGGCTTa             >  1:1778256/1‑68 (MQ=255)
                                                        ttGATGACGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCTTTACGGCTTa             >  1:2102945/1‑68 (MQ=255)
                                                        ttGATGACGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCTTTACGGCTTa             >  1:2829201/1‑68 (MQ=255)
                                                         tgatgaCGGTCGCTGGCCGCGCGATCCGTTTACACTGACGGAGCATGACGGCAAGCTTTACGGCTTAgg           >  1:337147/1‑69 (MQ=255)
                                                         tgatgaCGGTCGCTGGACGCGCGATCGGTTTACACTGACGGAGCATGACGGCAAGCTTTACGGCTTAgg           >  1:1644540/1‑69 (MQ=255)
                                                         tgatgaCGGTCGCTGGACGCGCGATCCGTTTACACTGACGGAGCATGACGGCAGGCTTTACGGCTTAgg           >  1:1960267/1‑69 (MQ=255)
                                                         tgatgaCGGTCGCTGGACGCGCGATCCGTTTACACTGACGGAGCATGACGGCAAGCTTTACGGCTTAgg           >  1:3255798/1‑69 (MQ=255)
                                                         tgatgaCGGTCGCTGGACGCGCGATCCGTTTACACTGACGGAGCATGACGGCAAGCTTTACGGCTTAgg           >  1:862529/1‑69 (MQ=255)
                                                         tgatgaCGGTCGCTGGACGCGCGATCCGTTTACACTGACGGAGCATGACGGCAAGCTTTACGGCTTAgg           >  1:3033238/1‑69 (MQ=255)
                                                         tgatgaCGGTCGCTGGACGCGCGATCCGTTTACACTGACGGAGCATGACGGCAAGCTTTACGGCTTAgg           >  1:1388528/1‑69 (MQ=255)
                                                          gatgaCGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCtt                      <  1:1251860/57‑1 (MQ=255)
                                                             gaCGGTCGCTGGACGCGCGATCCGTTTACACTGACGGAGCATGACGGCAAGCTTTACGGCTTAGGCAcc       <  1:2224640/69‑1 (MQ=255)
                                                             gaCGGTCGCTGGACGCGCGATCCGTTTACACTGACGGAGCATGACGGCAAGCTTTACGGCTTAGGCAcc       <  1:3336398/69‑1 (MQ=255)
                                                               cGGACGCTGGCCGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCTTTACGGCTTAGGCAccg      >  1:602110/1‑68 (MQ=255)
                                                               cGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCTTTACGGCTTAGGCAccg      >  1:1271417/1‑68 (MQ=255)
                                                               cGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCTTTACGGCTTAGGCAccg      >  1:1816432/1‑68 (MQ=255)
                                                               cGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCTTTACGGCTTAGGCAccg      >  1:703527/1‑68 (MQ=255)
                                                               cGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCTTTACGGCTTAGGCAccg      >  1:3077656/1‑68 (MQ=255)
                                                               cGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCTTTACGGCTTAGGCAccg      >  1:1107842/1‑68 (MQ=255)
                                                               cGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCTTTACGGCTTAGGCAccg      >  1:2367339/1‑68 (MQ=255)
                                                               cGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCTTTACGGCTTAGGCAccg      >  1:2585773/1‑68 (MQ=255)
                                                               cGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCTTTACGGCTTAGGCAccg      >  1:1664908/1‑68 (MQ=255)
                                                                  tCGCTGGACGCGCGATCCGTTTACACTGACGGAGCATGACGGCAAGCTTTACGGCTTAGGCACCGCCGa  <  1:1804441/69‑1 (MQ=255)
                                                                  |                                                                    
TATCGGACAGGGGGCTGGCGGCTTGTTGCTGGCGGGGCATACCGATACGGTGCCATTTGATGACGGTCGCTGGACGCGCGATCCGTTTACACTGACGGAGCATGACGGCAAGCTTTACGGCTTAGGCACCGCCGA  >  minE/2287740‑2287874

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: