Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,287,824 G→A 40.9% P95P (CCG→CCA argE → acetylornithine deacetylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,287,8240GA40.9% 54.7 / 86.7 122P95P (CCG→CCAargEacetylornithine deacetylase
Reads supporting (aligned to +/- strand):  ref base G (23/49);  new base A (30/20);  total (53/69)
Fisher's exact test for biased strand distribution p-value = 2.90e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.88e-01

Only 100 of 123 total aligned reads displayed.

GGCTTGTTGCTGGCGGGGCATACCGATACGGTGCCATTTGATGACGGTCGCTGGACGCGCGATCCGTTTACACTGACGGAGCATGACGGCAAGCTTTACGGCTTAGGCACCGCCGACATGAAAGGCTTT  >  minE/2287759‑2287887
                                                                 |                                                               
ggcTTGTTGCTGGCGGGGCATACCGATACGGTGCCATTTGATGACGGTCGCTGGACGCGCGATCCGtt                                                               <  1:106184/68‑1 (MQ=255)
ggcTTGTTGCTGGCGGGGCATACCGATACGGTGCCATTTGATGACGGTCGCTGGACGCGCGATCCGtt                                                               <  1:2008676/68‑1 (MQ=255)
 gcTTGTTGCTGGCGGGGCATACCGATACGGTGCCATTTGATGACGGTCGCTGGACTCGCGATCCGTTTa                                                             <  1:2238697/69‑1 (MQ=255)
 gcTTGTTGCTGGCGGGGCATACCGATACGGTGCCATTTGATGACGGTCGCTGGACGCGCGATCCGTTTa                                                             <  1:186694/69‑1 (MQ=255)
 gcTTGTTGCTGGCGGGGCATACCGATACGGTGCCATTTGATGACGGTCGCTGGACGCGCGATCCGTTTa                                                             <  1:3185652/69‑1 (MQ=255)
 gcTTGTTGCTGGCGGGGCATACCGATACGGTGCCATTTGATGACGGTCGCTGGACGCGCGATCCGTTTa                                                             <  1:2992238/69‑1 (MQ=255)
 gcTTGTTGCTGGCGGGGCATACCGATACGGTGCCATTTGATGACGGTCGCTGGACGCGCGATCCGTTTa                                                             <  1:642162/69‑1 (MQ=255)
 gcTTGTTGCTGGCGGGGCATACCGACACGGTGCCATTTGATGACGGTCGCTGGACGCGCGATCCGTTTa                                                             <  1:2604983/69‑1 (MQ=255)
  cTTGTTGCTGGCGGGGCATACCGATACGGTGCCATTTGATGACGGTCGCTGGACGCGCGATCCGTTTac                                                            <  1:296957/69‑1 (MQ=255)
  cTTGTTGCTGGCGGGGCATACCGATACGGTGCCATTTGATGACGGTCGCTGGACGCGCGATCCGTTTac                                                            <  1:2354553/69‑1 (MQ=255)
  cTTGTTGCTGGAGGGGCATACCGATACGGTGCCATTTGATGACGGTCGCTGGACGCGCGATCCGTTTac                                                            <  1:1015719/69‑1 (MQ=255)
   ttgttgCTGGCGGGGCATACCGATACGGTGCCATTTGATGACGGTCGCTGGACGCGCGATCCGTTTac                                                            <  1:631412/68‑1 (MQ=255)
               gggCATACCGATACGGTGCCATTTGATGACGGTCGCTGGACGCGCGATCCGTTTACACTGACGGAGCAt                                               <  1:3257407/69‑1 (MQ=255)
               gggCATACCGATACGGTGCCATTTGATGACGGTCGCTGGACGCGCGATCCGTTTACACTGACGGAGCAt                                               <  1:2116588/69‑1 (MQ=255)
                   aTACCGATACGGTGCCATTTGATGACGGTCGCTGGACGCGCGATCCGTTTACACTGACGGAg                                                  <  1:96267/62‑1 (MQ=255)
                      ccGATACGGTGCCATTTGATGACGGTCGCTGGACGCGCGATCCGTTTACACTGACGGAGCATGACGGCa                                        >  1:744708/1‑69 (MQ=255)
                      ccGATACGGTGCCATTTGATGACGGTCGCTGGACGCGCGATCCGTTTACACTGACGGAGCATGACGGCa                                        >  1:1939200/1‑69 (MQ=255)
                      ccGATACGGTGCCATTTGATGACGGTCGCTGGACGCGCGATCCGTTTACACTGACGGAGCATGACGGCa                                        >  1:795270/1‑69 (MQ=255)
                      ccGATACGGTGCCATTTGATGACGGTCGCTGGACGCGCGATCCGTTTACACTGACGGAGCATGACGGCa                                        >  1:770817/1‑69 (MQ=255)
                      ccGATACGGTGCCATTTGATGACGGTCGCTGGACGCGCGATCCGTTTACACTGACGGAGCATGACGGCa                                        >  1:2314431/1‑69 (MQ=255)
                      ccGATACGGTGCCATTTGATGACGGTCGCTGGACGCGCGATCCGTTTACACTGACGGAGCATGACGGCa                                        >  1:389125/1‑69 (MQ=255)
                      ccGATACGGTGCCATTTGATGACGGTCGCTGGACGCGCGATCCGTTTACACTGACGGAGCATGACGGCa                                        >  1:290533/1‑69 (MQ=255)
                      ccGATACGGTGCCATTTGATGACGGTCGCTGGACGCGCGATCCGTTTACACTGACGGAGCATGACGGCa                                        >  1:2786214/1‑69 (MQ=255)
                      ccGATACGGTGCCATTTGATGACGGTCGCTGGACGCGCGATCCGTTTACACTGACGGAGCATGACGGCa                                        >  1:282035/1‑69 (MQ=255)
                      ccGATACGGTGCCATTTGATGACGGTCGCTGGACGCGCGATCCGTTTACACTGACGGAGCATGACGGCa                                        >  1:591909/1‑69 (MQ=255)
                           aCGGTGCCATTTGATGACGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCtt                                   <  1:2162786/69‑1 (MQ=255)
                           aCGGTGCCATTTGATGACGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCtt                                   <  1:2009935/69‑1 (MQ=255)
                           aCGGTGCCATTTGATGACGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCtt                                   <  1:3042362/69‑1 (MQ=255)
                           aCGGTGCCATTTGATGACGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCtt                                   <  1:2598903/69‑1 (MQ=255)
                           aCGGTGCCATTTGATGACGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCtt                                   <  1:3298319/69‑1 (MQ=255)
                           aCGGTGCCATTTGATGACGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCtt                                   <  1:1100472/69‑1 (MQ=255)
                           aCGGTGCCATTTGATGACGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCtt                                   <  1:3336505/69‑1 (MQ=255)
                           aCGGTGCCATTTGATGACGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCtt                                   <  1:368109/69‑1 (MQ=255)
                           aCGGTGCCATTTGATGACGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCtt                                   <  1:658918/69‑1 (MQ=255)
                           aCGGTGCCATTTGATGACGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCtt                                   <  1:1935473/69‑1 (MQ=255)
                           aCGGTGCCATTTGATGACGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCtt                                   <  1:559167/69‑1 (MQ=255)
                           aCGGTGCCATTTGATGACGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCtt                                   <  1:1297888/69‑1 (MQ=255)
                           aCGGTGCCATTTGATGACGGACGCTGGACGCGCGATCCATTTACGTTGAAGGAGCATGACGGCAAGCtt                                   <  1:2093092/69‑1 (MQ=255)
                                gCCATTTGATGACGGTCGCTGGACGCGCGATCCGTTTACACTGACGGAg                                                  >  1:2776493/1‑49 (MQ=255)
                                gCCATTTGATGACGGTCGCTGGACGCGCGATCCGTTTACACTGACGGAg                                                  >  1:2996019/1‑49 (MQ=255)
                                gCCATTTGATGACGGTCGCTGGACGCGCGATCCGTTTACACTGACGGAg                                                  >  1:2486725/1‑49 (MQ=255)
                                gCCATTTGATGACGGTCGCTGGACGCGCGATCCGTTTACACTGACGGAg                                                  >  1:1234201/1‑49 (MQ=255)
                                 ccATTTGATGACGGTCGCTGGACGCGCGATCCGTTTACACTGACGGAGCATGACGGCAAGCTTTACGGc                             <  1:1706786/69‑1 (MQ=255)
                                 ccATTTGATGACGGTCGCTGGACGCGCGATCCGTTTACACTGACGGAGCATGACGGCAAGCTTTACGGc                             <  1:2464681/69‑1 (MQ=255)
                                     ttGATGACGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCTTTACGGCTTa                          >  1:2688668/1‑68 (MQ=255)
                                     ttGATGACGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCTTTACGGCTTa                          >  1:533846/1‑68 (MQ=255)
                                     ttGATGACGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCTTTACGGCTTa                          >  1:1022743/1‑68 (MQ=255)
                                     ttGATGACGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCTTTACGGCTTa                          >  1:635958/1‑68 (MQ=255)
                                     ttGATGACGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCTTTACGGCTTa                          >  1:507934/1‑68 (MQ=255)
                                     ttGATGACGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCTTTACGGCTTa                          >  1:499361/1‑68 (MQ=255)
                                     ttGATGACGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCTTTACGGCTTa                          >  1:3319377/1‑68 (MQ=255)
                                     ttGATGACGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCTTTACGGCTTa                          >  1:3196043/1‑68 (MQ=255)
                                     ttGATGACGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCTTTACGGCTTa                          >  1:1049989/1‑68 (MQ=255)
                                     ttGATGACGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCTTTACGGCTTa                          >  1:2501953/1‑68 (MQ=255)
                                     ttGATGACGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCTTTACGGCTTa                          >  1:1098310/1‑68 (MQ=255)
                                     ttGATGACGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCTTTACGGCTTa                          >  1:1235976/1‑68 (MQ=255)
                                     ttGATGACGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCTTTACGGCTTa                          >  1:1329895/1‑68 (MQ=255)
                                     ttGATGACGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCTTTACGGCTTa                          >  1:1709977/1‑68 (MQ=255)
                                     ttGATGACGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCTTTACGGCTTa                          >  1:2102945/1‑68 (MQ=255)
                                     ttGATGACGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCTTTACGGCTTa                          >  1:1710545/1‑68 (MQ=255)
                                     ttGATGACGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCTTTACGGCTTa                          >  1:1778256/1‑68 (MQ=255)
                                      tgatgaCGGTCGCTGGCCGCGCGATCCGTTTACACTGACGGAGCATGACGGCAAGCTTTACGGCTTAgg                        >  1:337147/1‑69 (MQ=255)
                                      tgatgaCGGTCGCTGGACGCGCGATCCGTTTACACTGACGGAGCATGACGGCAAGCTTTACGGCTTAgg                        >  1:862529/1‑69 (MQ=255)
                                      tgatgaCGGTCGCTGGACGCGCGATCCGTTTACACTGACGGAGCATGACGGCAAGCTTTACGGCTTAgg                        >  1:1388528/1‑69 (MQ=255)
                                      tgatgaCGGTCGCTGGACGCGCGATCCGTTTACACTGACGGAGCATGACGGCAAGCTTTACGGCTTAgg                        >  1:3255798/1‑69 (MQ=255)
                                      tgatgaCGGTCGCTGGACGCGCGATCCGTTTACACTGACGGAGCATGACGGCAAGCTTTACGGCTTAgg                        >  1:3033238/1‑69 (MQ=255)
                                       gatgaCGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCtt                                   <  1:1251860/57‑1 (MQ=255)
                                          gaCGGTCGCTGGACGCGCGATCCGTTTACACTGACGGAGCATGACGGCAAGCTTTACGGCTTAGGCAcc                    <  1:3336398/69‑1 (MQ=255)
                                          gaCGGTCGCTGGACGCGCGATCCGTTTACACTGACGGAGCATGACGGCAAGCTTTACGGCTTAGGCAcc                    <  1:2224640/69‑1 (MQ=255)
                                            cGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCTTTACGGCTTAGGCAccg                   >  1:3077656/1‑68 (MQ=255)
                                            cGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCTTTACGGCTTAGGCAccg                   >  1:1816432/1‑68 (MQ=255)
                                            cGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCTTTACGGCTTAGGCAccg                   >  1:1664908/1‑68 (MQ=255)
                                            cGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCTTTACGGCTTAGGCAccg                   >  1:2585773/1‑68 (MQ=255)
                                            cGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCTTTACGGCTTAGGCAccg                   >  1:703527/1‑68 (MQ=255)
                                            cGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCTTTACGGCTTAGGCAccg                   >  1:1107842/1‑68 (MQ=255)
                                            cGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCTTTACGGCTTAGGCAccg                   >  1:2367339/1‑68 (MQ=255)
                                                  cTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCtt                                   <  1:1070510/46‑1 (MQ=255)
                                                   tGGACGCGCGATCCGTTTACACTGACGGAGCATGACGGCAAGCTTTACGGCTTAGGCACCGCCGACATg           <  1:1372866/69‑1 (MQ=255)
                                                   tGGACGCGCGATCCGTTTACACTGACGGAGCATGACGGCAAGCTTTACGGCTTAGGCACCGCCGACATg           <  1:773036/69‑1 (MQ=255)
                                                   tGGACGCGCGATCCGTTTACACTGACGGAGCATGACGGCAAGCTTTACGGCTTAGGCACCGCCGACATg           <  1:2689933/69‑1 (MQ=255)
                                                   tGGACGCGCGATCCGTTTACACTGACGGAGCATGACGGCAAGCTTTACGGCTTAGGCACCGCCGACATg           <  1:1104770/69‑1 (MQ=255)
                                                   tGGACGCGCGATCCGTTTACACTGACGGAGCATGACGGCAAGCTTTACGGCTTAGGCACCGCCGACATg           <  1:478430/69‑1 (MQ=255)
                                                   tGGACGCGCGATCCGTTTACACTGACGGAGCATGACGGCAAGCTTTACGGCTTAGGCACCGCCGACATg           <  1:1583236/69‑1 (MQ=255)
                                                         cgcgATCCGTTTACACTGACGGAGCATGACGGCAAGCTTTACGGCTTAGGCACCGCCGACATTAAAGGc     <  1:1450628/69‑1 (MQ=255)
                                                         cgcgATCCGTTTACACTGACGGAGCATGACGGCAAGCTTTACGGCTTAGGCACCGCCGACATGAAAGGc     <  1:2405244/69‑1 (MQ=255)
                                                         cgcgATCCGTTTACACTGACGGAGCATGACGGCAAGCTTTACGGCTTAGGCACCGCCGACATGAAAGGc     <  1:1239639/69‑1 (MQ=255)
                                                         cgcgATCCGTTTACACTGACGGAGCATGACGGCAAGCTTTACGGCTTAGGCACCGCCGACATGAAAGGc     <  1:2765179/69‑1 (MQ=255)
                                                         cgcgATCCGTTTACACTGACGGAGCATGACGGCAAGCTTTACGGCTTAGGCACCGCCGACATGAAAGGc     <  1:2889732/69‑1 (MQ=255)
                                                         cgcgATCCGTTTACACTGACGGAGCATGACGGCAAGCTTTACGGCTTAGGCACCGCCGACATGAAAGGc     <  1:2977992/69‑1 (MQ=255)
                                                         cgcgATCCGTTTACACTGACGGAGCATGACGGCAAGCTTTACGGCTTAGGCACCGCCGACATGAAAGGc     <  1:2074862/69‑1 (MQ=255)
                                                          gcgATCCGTTTACACTGACGGAGCATGACGGCAAGCTTTACGGCTTAGGCACCGCCGACATGAAAGGc     <  1:2276782/68‑1 (MQ=255)
                                                          gcgATCCGTTTACACTGACGGAGCATGACGGCAAGCTTTACGGCTTAGGCACCGCCGACATGAAAGGc     <  1:2677160/68‑1 (MQ=255)
                                                          gcgATCCGTTTACACTGACGGAGCATGACGGCAAGCTTTACGGCTTAGGCACCGCCGACATGAAAGGc     <  1:900709/68‑1 (MQ=255)
                                                          gcgATCCGTTTACACTGACGGAGCATGACGGCAAGCTTTACGGCTTAGGCACCGCCGACATGAAAGGc     <  1:932949/68‑1 (MQ=255)
                                                          gcgATCCGTTTACACTGACGGAGCATGACGGCAAGCTTTACGGCTTAGGCACCGCCGACATGAAAGGc     <  1:2673158/68‑1 (MQ=255)
                                                          gcgATCCGTTTACACTGACGGAGCATGACGGCAAGCTTTACGGCTTAGGCACCGCCGACATGAAAGGCt    <  1:1983589/69‑1 (MQ=255)
                                                          gcgATCCGTTTACACTGACGGAGCATGACGGCAAGCTTTACGGCTTAGGCACCGCCGACATGAAAGGCt    <  1:1992074/69‑1 (MQ=255)
                                                            gATCCGTTTACACTGACGGAGCATGACGGCAAGCTTTACGGCTTAGGCACCGCCGACATGAAAGGCttt  <  1:1510214/69‑1 (MQ=255)
                                                            gATCCGTTTACACTGACGGAGCATGACGGCAAGCTTTACGGCTTAGGCACCGCCGACATGAAAGGCttt  <  1:1622201/69‑1 (MQ=255)
                                                                 gTTTACACTGACGGAGCATGACGGCAAGCTTTACGGCTTAGGCACCGCCGACATGAAAGGc     <  1:1234991/61‑1 (MQ=255)
                                                                 |                                                               
GGCTTGTTGCTGGCGGGGCATACCGATACGGTGCCATTTGATGACGGTCGCTGGACGCGCGATCCGTTTACACTGACGGAGCATGACGGCAAGCTTTACGGCTTAGGCACCGCCGACATGAAAGGCTTT  >  minE/2287759‑2287887

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: