Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,287,831 C→T 41.6% L98L (CTG→TTG)  argE → acetylornithine deacetylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,287,8310CT41.6% 49.6 / 87.3 120L98L (CTG→TTG) argEacetylornithine deacetylase
Reads supporting (aligned to +/- strand):  ref base C (35/35);  new base T (30/20);  total (65/55)
Fisher's exact test for biased strand distribution p-value = 3.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01

Only 100 of 121 total aligned reads displayed.

GGGCATACCGATACGGTGCCATTTGATGACGGTCGCTGGACGCGCGATCCGTTTACACTGACGGAGCATGACGGCAAGCTTTACGGCTTAGGCACCGCCGACATGAAAGGCTTTTTTGCGTTTATCC  >  minE/2287774‑2287900
                                                         |                                                                     
gggCATACCGATACGGTGCCATTTGATGACGGTCGCTGGACGCGCGATCCGTTTACACTGACGGAGCAt                                                            <  1:3257407/69‑1 (MQ=255)
gggCATACCGATACGGTGCCATTTGATGACGGTCGCTGGACGCGCGATCCGTTTACACTGACGGAGCAt                                                            <  1:2116588/69‑1 (MQ=255)
    aTACCGATACGGTGCCATTTGATGACGGTCGCTGGACGCGCGATCCGTTTACACTGACGGAg                                                               <  1:96267/62‑1 (MQ=255)
       ccGATACGGTGCCATTTGATGACGGTCGCTGGACGCGCGATCCGTTTACACTGACGGAGCATGACGGCa                                                     >  1:591909/1‑69 (MQ=255)
       ccGATACGGTGCCATTTGATGACGGTCGCTGGACGCGCGATCCGTTTACACTGACGGAGCATGACGGCa                                                     >  1:290533/1‑69 (MQ=255)
       ccGATACGGTGCCATTTGATGACGGTCGCTGGACGCGCGATCCGTTTACACTGACGGAGCATGACGGCa                                                     >  1:282035/1‑69 (MQ=255)
       ccGATACGGTGCCATTTGATGACGGTCGCTGGACGCGCGATCCGTTTACACTGACGGAGCATGACGGCa                                                     >  1:2786214/1‑69 (MQ=255)
       ccGATACGGTGCCATTTGATGACGGTCGCTGGACGCGCGATCCGTTTACACTGACGGAGCATGACGGCa                                                     >  1:2657080/1‑69 (MQ=255)
       ccGATACGGTGCCATTTGATGACGGTCGCTGGACGCGCGATCCGTTTACACTGACGGAGCATGACGGCa                                                     >  1:389125/1‑69 (MQ=255)
       ccGATACGGTGCCATTTGATGACGGTCGCTGGACGCGCGATCCGTTTACACTGACGGAGCATGACGGCa                                                     >  1:2314431/1‑69 (MQ=255)
       ccGATACGGTGCCATTTGATGACGGTCGCTGGACGCGCGATCCGTTTACACTGACGGAGCATGACGGCa                                                     >  1:1939200/1‑69 (MQ=255)
       ccGATACGGTGCCATTTGATGACGGTCGCTGGACGCGCGATCCGTTTACACTGACGGAGCATGACGGCa                                                     >  1:744708/1‑69 (MQ=255)
       ccGATACGGTGCCATTTGATGACGGTCGCTGGACGCGCGATCCGTTTACACTGACGGAGCATGACGGCa                                                     >  1:770817/1‑69 (MQ=255)
       ccGATACGGTGCCATTTGATGACGGTCGCTGGACGCGCGATCCGTTTACACTGACGGAGCATGACGGCa                                                     >  1:795270/1‑69 (MQ=255)
            aCGGTGCCATTTGATGACGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCtt                                                <  1:3336505/69‑1 (MQ=255)
            aCGGTGCCATTTGATGACGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCtt                                                <  1:271395/69‑1 (MQ=255)
            aCGGTGCCATTTGATGACGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCtt                                                <  1:1935473/69‑1 (MQ=255)
            aCGGTGCCATTTGATGACGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCtt                                                <  1:2995223/69‑1 (MQ=255)
            aCGGTGCCATTTGATGACGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCtt                                                <  1:3298319/69‑1 (MQ=255)
            aCGGTGCCATTTGATGACGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCtt                                                <  1:2598903/69‑1 (MQ=255)
            aCGGTGCCATTTGATGACGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCtt                                                <  1:368109/69‑1 (MQ=255)
            aCGGTGCCATTTGATGACGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCtt                                                <  1:559167/69‑1 (MQ=255)
            aCGGTGCCATTTGATGACGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCtt                                                <  1:658918/69‑1 (MQ=255)
            aCGGTGCCATTTGATGACGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCtt                                                <  1:710851/69‑1 (MQ=255)
            aCGGTGCCATTTGATGACGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCtt                                                <  1:967607/69‑1 (MQ=255)
            aCGGTGCCATTTGATGACGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCtt                                                <  1:1297888/69‑1 (MQ=255)
            aCGGTGCCATTTGATGACGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCtt                                                <  1:1100472/69‑1 (MQ=255)
            aCGGTGCCATTTGATGACGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCtt                                                <  1:1210523/69‑1 (MQ=255)
            aCGGTGCCATTTGATGACGGACGCTGGACGCGCGATCCATTTACGTTGAAGGAGCATGACGGCAAGCtt                                                <  1:2093092/69‑1 (MQ=255)
                 gCCATTTGATGACGGTCGCTGGACGCGCGATCCGTTTACACTGACGGAg                                                               >  1:2486725/1‑49 (MQ=255)
                 gCCATTTGATGACGGTCGCTGGACGCGCGATCCGTTTACACTGACGGAg                                                               >  1:2996019/1‑49 (MQ=255)
                 gCCATTTGATGACGGTCGCTGGACGCGCGATCCGTTTACACTGACGGAg                                                               >  1:1234201/1‑49 (MQ=255)
                  ccATTTGATGACGGTCGCTGGACGCGCGATCCGTTTACACTGACGGAGCATGACGGCAAGCTTTACGGc                                          <  1:1706786/69‑1 (MQ=255)
                  ccATTTGATGACGGTCGCTGGACGCGCGATCCGTTTACACTGACGGAGCATGACGGCAAGCTTTACGGc                                          <  1:2464681/69‑1 (MQ=255)
                      ttGATGACGGACGCTGGACGCGCGATCCATTTACGTTGACGGAg                                                               >  1:816020/1‑44 (MQ=255)
                      ttGATGACGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCTTTACGGCTTa                                       >  1:635958/1‑68 (MQ=255)
                      ttGATGACGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCTTTACGGCTTa                                       >  1:533846/1‑68 (MQ=255)
                      ttGATGACGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCTTTACGGCTTa                                       >  1:507934/1‑68 (MQ=255)
                      ttGATGACGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCTTTACGGCTTa                                       >  1:507753/1‑68 (MQ=255)
                      ttGATGACGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCTTTACGGCTTa                                       >  1:1235976/1‑68 (MQ=255)
                      ttGATGACGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCTTTACGGCTTa                                       >  1:3319377/1‑68 (MQ=255)
                      ttGATGACGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCTTTACGGCTTa                                       >  1:3196043/1‑68 (MQ=255)
                      ttGATGACGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCTTTACGGCTTa                                       >  1:1049989/1‑68 (MQ=255)
                      ttGATGACGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCTTTACGGCTTa                                       >  1:2829201/1‑68 (MQ=255)
                      ttGATGACGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCTTTACGGCTTa                                       >  1:2688668/1‑68 (MQ=255)
                      ttGATGACGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCTTTACGGCTTa                                       >  1:1022743/1‑68 (MQ=255)
                      ttGATGACGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCTTTACGGCTTa                                       >  1:1710545/1‑68 (MQ=255)
                      ttGATGACGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCTTTACGGCTTa                                       >  1:1709977/1‑68 (MQ=255)
                      ttGATGACGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCTTTACGGCTTa                                       >  1:1778256/1‑68 (MQ=255)
                      ttGATGACGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCTTTACGGCTTa                                       >  1:2102945/1‑68 (MQ=255)
                      ttGATGACGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCTTTACGGCTTa                                       >  1:2476565/1‑68 (MQ=255)
                       tgatgaCGGTCGCTGGCCGCGCGATCCGTTTACACTGACGGAGCATGACGGCAAGCTTTACGGCTTAgg                                     >  1:337147/1‑69 (MQ=255)
                       tgatgaCGGTCGCTGGACGCGCGATCGGTTTACACTGACGGAGCATGACGGCAAGCTTTACGGCTTAgg                                     >  1:1644540/1‑69 (MQ=255)
                       tgatgaCGGTCGCTGGACGCGCGATCCGTTTACACTGACGGAGCATGACGGCAGGCTTTACGGCTTAgg                                     >  1:1960267/1‑69 (MQ=255)
                       tgatgaCGGTCGCTGGACGCGCGATCCGTTTACACTGACGGAGCATGACGGCAAGCTTTACGGCTTAgg                                     >  1:3255798/1‑69 (MQ=255)
                       tgatgaCGGTCGCTGGACGCGCGATCCGTTTACACTGACGGAGCATGACGGCAAGCTTTACGGCTTAgg                                     >  1:3033238/1‑69 (MQ=255)
                       tgatgaCGGTCGCTGGACGCGCGATCCGTTTACACTGACGGAGCATGACGGCAAGCTTTACGGCTTAgg                                     >  1:1388528/1‑69 (MQ=255)
                        gatgaCGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCtt                                                <  1:1251860/57‑1 (MQ=255)
                           gaCGGTCGCTGGACGCGCGATCCGTTTACACTGACGGAGCATGACGGCAAGCTTTACGGCTTAGGCAcc                                 <  1:3336398/69‑1 (MQ=255)
                           gaCGGTCGCTGGACGCGCGATCCGTTTACACTGACGGAGCATGACGGCAAGCTTTACGGCTTAGGCAcc                                 <  1:2224640/69‑1 (MQ=255)
                             cGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCTTTACGGCTTAGGCAccg                                >  1:1664908/1‑68 (MQ=255)
                             cGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCTTTACGGCTTAGGCAccg                                >  1:1271417/1‑68 (MQ=255)
                             cGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCTTTACGGCTTAGGCAccg                                >  1:3077656/1‑68 (MQ=255)
                             cGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCTTTACGGCTTAGGCAccg                                >  1:1107842/1‑68 (MQ=255)
                             cGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCTTTACGGCTTAGGCAccg                                >  1:703527/1‑68 (MQ=255)
                             cGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCTTTACGGCTTAGGCAccg                                >  1:2585773/1‑68 (MQ=255)
                             cGGACGCTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCTTTACGGCTTAGGCAccg                                >  1:2367339/1‑68 (MQ=255)
                                tCGCTGGACGCGCGATCCGTTTACACTGACGGAGCATGACGGCAAGCTTTACGGCTTAGGCACCGCCGa                            <  1:1804441/69‑1 (MQ=255)
                                   cTGGACGCGCGATCCATTTACGTTGACGGAGCATGACGGCAAGCtt                                                <  1:1070510/46‑1 (MQ=255)
                                    tGGACGCGCGATCCGTTTACACTGACGGAGCATGACGGCAAGCTTTACGGCTTAGGCACCGCCGACATg                        <  1:1583236/69‑1 (MQ=255)
                                    tGGACGCGCGATCCGTTTACACTGACGGAGCATGACGGCAAGCTTTACGGCTTAGGCACCGCCGACATg                        <  1:478430/69‑1 (MQ=255)
                                    tGGACGCGCGATCCGTTTACACTGACGGAGCATGACGGCAAGCTTTACGGCTTAGGCACCGCCGACATg                        <  1:1104770/69‑1 (MQ=255)
                                    tGGACGCGCGATCCGTTTACACTGACGGAGCATGACGGCAAGCTTTACGGCTTAGGCACCGCCGACATg                        <  1:773036/69‑1 (MQ=255)
                                    tGGACGCGCGATCCGTTTACACTGACGGAGCATGACGGCAAGCTTTACGGCTTAGGCACCGCCGACATg                        <  1:2689933/69‑1 (MQ=255)
                                    tGGACGCGCGATCCGTTTACACTGACGGAGCATGACGGCAAGCTTTACGGCTTAGGCACCGCCGACATg                        <  1:1372866/69‑1 (MQ=255)
                                          cgcgATCCGTTTACACTGACGGAGCATGACGGCAAGCTTTACGGCTTAGGCACCGCCGACATTAAAGGc                  <  1:1450628/69‑1 (MQ=255)
                                          cgcgATCCGTTTACACTGACGGAGCATGACGGCAAGCTTTACGGCTTAGGCACCGCCGACATGAAAGGc                  <  1:2765179/69‑1 (MQ=255)
                                          cgcgATCCGTTTACACTGACGGAGCATGACGGCAAGCTTTACGGCTTAGGCACCGCCGACATGAAAGGc                  <  1:1239639/69‑1 (MQ=255)
                                          cgcgATCCGTTTACACTGACGGAGCATGACGGCAAGCTTTACGGCTTAGGCACCGCCGACATGAAAGGc                  <  1:2405244/69‑1 (MQ=255)
                                          cgcgATCCGTTTACACTGACGGAGCATGACGGCAAGCTTTACGGCTTAGGCACCGCCGACATGAAAGGc                  <  1:2977992/69‑1 (MQ=255)
                                          cgcgATCCGTTTACACTGACGGAGCATGACGGCAAGCTTTACGGCTTAGGCACCGCCGACATGAAAGGc                  <  1:2074862/69‑1 (MQ=255)
                                           gcgATCCGTTTACACTGACGGAGCATGACGGCAAGCTTTACGGCTTAGGCACCGCCGACATGAAAGGc                  <  1:2673158/68‑1 (MQ=255)
                                           gcgATCCGTTTACACTGACGGAGCATGACGGCAAGCTTTACGGCTTAGGCACCGCCGACATGAAAGGc                  <  1:900709/68‑1 (MQ=255)
                                           gcgATCCGTTTACACTGACGGAGCATGACGGCAAGCTTTACGGCTTAGGCACCGCCGACATGAAAGGc                  <  1:932949/68‑1 (MQ=255)
                                           gcgATCCGTTTACACTGACGGAGCATGACGGCAAGCTTTACGGCTTAGGCACCGCCGACATGAAAGGc                  <  1:2276782/68‑1 (MQ=255)
                                           gcgATCCGTTTACACTGACGGAGCATGACGGCAAGCTTTACGGCTTAGGCACCGCCGACATGAAAGGc                  <  1:2677160/68‑1 (MQ=255)
                                           gcgATCCGTTTACACTGACGGAGCATGACGGCAAGCTTTACGGCTTAGGCACCGCCGACATGAAAGGCt                 <  1:1992074/69‑1 (MQ=255)
                                           gcgATCCGTTTACACTGACGGAGCATGACGGCAAGCTTTACGGCTTAGGCACCGCCGACATGAAAGGCt                 <  1:1983589/69‑1 (MQ=255)
                                           gcgATCCGTTTACACTGACGGAGCATGACGGCAAGCTTTACGGCTTAGGCACCGCCGACATGAAAGGCt                 <  1:3121518/69‑1 (MQ=255)
                                           gcgATCCGTTTACACTGACGGAGCATGACGGCAAGCTGTACGGCTTAGGCACCGCCGa                            <  1:1088301/58‑1 (MQ=255)
                                             gATCCGTTTACACTGACGGAGCATGACGGCAAGCTTTACGGCTTAGGCACCGCCGACATGAAAGGCttt               <  1:1622201/69‑1 (MQ=255)
                                             gATCCGTTTACACTGACGGAGCATGACGGCAAGCTTTACGGCTTAGGCACCGCCGACATGAAAGGCttt               <  1:1510214/69‑1 (MQ=255)
                                                     tACACTGACGGAGCATGACGGCAAGCTTTACGGCTTAGGCACCGCCGACATGAAAGGCTTTTTTGCgct       >  1:2801907/1‑67 (MQ=255)
                                                     tACACTGACGGAGCATGACGGCAAGCTTTACGGCTTAGGCACCGCCGACATGAAAGGCTTTTTTGCGtt       >  1:1059002/1‑69 (MQ=255)
                                                     tACACTGACGGAGCATGACGGCAAGCTTTACGGCTTAGGCACCGCCGACATGAAAGGCTTTTTTGCGtt       >  1:2188600/1‑69 (MQ=255)
                                                     tACACTGACGGAGCATGACGGCAAGCTTTACGGCTTAGGCACCGCCGACATGAAAGGCTTTTTTGCGtt       >  1:833432/1‑69 (MQ=255)
                                                     tACACTGACGGAGCATGACGGCAAGCTTTACGGCTTAGGCACCGCCGACATGAAAGGCTTTTTTGCGtt       >  1:1475408/1‑69 (MQ=255)
                                                     tACACTGACGGAGCATGACGGCAAGCTTTACGGCTTAGGCACCGCCGACATGAAAGGCTTTTTTGCGtt       >  1:2070389/1‑69 (MQ=255)
                                                     tACACTGACGGAGCATGACGGCAAGCTTTACGGCTTAGGCACCGCCGACATGAAAGGCTTTTTTGCGtt       >  1:380282/1‑69 (MQ=255)
                                                         cTGACGGAGCATGACGGCAAGCTTTACGGCTTAGGCACCGCCGACATGAAAGGCTTTTTTCGTTTATcc  >  1:2528984/1‑69 (MQ=255)
                                                         |                                                                     
GGGCATACCGATACGGTGCCATTTGATGACGGTCGCTGGACGCGCGATCCGTTTACACTGACGGAGCATGACGGCAAGCTTTACGGCTTAGGCACCGCCGACATGAAAGGCTTTTTTGCGTTTATCC  >  minE/2287774‑2287900

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: