| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 193,541 | A→G | 28.0% | V142V (GTA→GTG) | yaeQ → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 193,541 | 0 | A | G | 28.0% | 138.6 / 58.2 | 133 | V142V (GTA→GTG) | yaeQ | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (44/51); new base G (25/12); total (69/64) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.36e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 134 total aligned reads displayed.
TGCAGTTTGCCAATCTTTCCGTCTGGTATCTGGACGATGAACAACTGGCGAAAGTAAGCGCCTTTGCCGATCGTACCATGACGCTGCAGGCAACGATTCAGGATGGCGTGATCTG > minE/193486‑193600 | tgCAGTTTGCCAATCTTTCCGTCTGGTATCTGGACGATGAACAACTGGCGAAAGTAAGCGCCTTTGCCg < 1:2567305/69‑1 (MQ=255)tgCAGTTTGCCAATCTTTCCGTCTGGTATCTGGACGATGAACAACTGGCGAAAGTAAGCGCCTTTGCCg < 1:1305051/69‑1 (MQ=255)tgCAGTTTGCCAATCTTTCCGTCTGGTATCTGGACGATGAACAACTGGCGAAAGTAAGCGCCTTTGCCg < 1:2772439/69‑1 (MQ=255) cAGTTTGCCAATCTTTCCGTCTGGTATCTGGACGATGAACAACTGGCGAAAGTAAGCGCCTTTGCCGAt < 1:2921213/69‑1 (MQ=255) cAGTTTGCCAATCTTTCCGTCTGGTATCTGGACGATGAACAACTGGCGAAAGTAAGCGCCTTTGCCGAt < 1:24839/69‑1 (MQ=255) cAGTTTGCCAATCTTTCCGTCTGGTATCTGGACGATGAACAACTGGCGAAAGTAAGCGCCTTTGCCGAt < 1:151048/69‑1 (MQ=255) tGCCAATCTTTCCGTCTGGTATCTGGACGATGAACAACTGGCGAAAGTAAGCGCCTTTGCCGATCGTa > 1:176244/1‑68 (MQ=255) tGCCAATCTTTCCGTCTGGTATCTGGACGATGAACAACTGGCGAAAGTAAGCGCCTTTGCCGATCGTAc > 1:2752304/1‑69 (MQ=255) tGCCAATCTTTCCGTCTGGTATCTGGACGATGAACAACTGGCGAAAGTAAGCGCCTTTGCCGATCGTAc > 1:3198483/1‑69 (MQ=255) tGCCAATCTTTCCGTCTGGTATCTGGACGATGAACAACTGGCGAAAGTAAGCGCCTTTGCCGATCGTAc > 1:3030052/1‑69 (MQ=255) tGCCAATCTTTCCGTCTGGTATCTGGACGATGAACAACTGGCGAAAGTAAGCGCCTTTGCCGATCGTAc > 1:2897254/1‑69 (MQ=255) tGCCAATCTTTCCGTCTGGTATCTGGACGATGAACAACTGGCGAAAGTAAGCGCCTTTGCCGATCGTAc > 1:2158222/1‑69 (MQ=255) tGCCAATCTTTCCGTCTGGTATCTGGACGATGAACAACTGGCGAAAGTAAGCGCCTTTGCCGATCGTAc > 1:23479/1‑69 (MQ=255) tGCCAATCTTTCCGTCTGGTATCTGGACGATGAACAACTGGCGAAAGTAAGCGCCTTTGCCGATCGTAc > 1:391002/1‑69 (MQ=255) tGCCAATCTTTCCGTCTGGTATCTGGACGATGAACAACTGGCGAAAGTAAGCGCCTTTGCCGATCGTAc > 1:472404/1‑69 (MQ=255) tGCCAATCTTTCCGTCTGGTATCTGGACGATGAACAACTGGCGAAAGTAAGCGCCTTTGCCGATCGTAc > 1:60292/1‑69 (MQ=255) tGCCAATCTTTCCGTCTGGTATCTGGACGATGAACAACTGGCGAAAGTAAGCGCCTTTGCCGATCGTAc > 1:11639/1‑69 (MQ=255) tGCCAATCTTTCCGTCTGGTATCTGGACGATGAACAACTGGCGAAAGTAAGCGCCTTTGCCGATCGTAc > 1:2702906/1‑69 (MQ=255) tGCCAATCTTTCCGTCTGGTATCTGGACGATGAACAACTGGCGAAAGTAAGCGCCTTTGCCGATCGTAc > 1:1073935/1‑69 (MQ=255) tGCCAATCTTTCCGTCTGGTATCTGGACGATGAACAACTGGCGAAAGTAAGCGCATTTGCCGATCGTAc > 1:685934/1‑69 (MQ=255) aTCTTTCCGTCTGGTATCTGGACGACGAGCAGCTGGCGAAAGTGAGCGGCTTTGCCGATCGt < 1:826673/62‑1 (MQ=255) aTCTTTCCGTCTGGTATCTGGACGACGAGCAGCTGGCGAAAGTGAGCGGCTTTGCCGATCGt < 1:3189650/62‑1 (MQ=255) aTCTTTCCGTCTGGTATCTGGACGACGAGCAGCTGGCGAAAGTGAGCGGCTTTGCCGATCGt < 1:2643505/62‑1 (MQ=255) aTCTTTCCGTCTGGTATCTGGACGACGAGCAGCTGGCGAAAGTGAGCGGCTTTGCCGATCGt < 1:1882635/62‑1 (MQ=255) aTCTTTCCGTCTGGTATCTGGACGACGAGCAGCTGGCGAAAGTGAGCGGCTTTGCCGATCGt < 1:342669/62‑1 (MQ=255) gTATCTGGACGATGAACAACTGGCGAAAGTAAGCGCCTTTGCCGATCGTACCATGACGCTGCAGGCAAc > 1:3140582/1‑69 (MQ=255) gTATCTGGACGATGAACAACTGGCGAAAGTAAGCGCCTTTGCCGATCGTACCATGACGCTGCAGGCAAc > 1:2206127/1‑69 (MQ=255) aTCTGTACGATGAACAACTGGCGAAAGTAAGCGCCTTTGCCGATCGTACCATGACGCTGCAGGCAACGa > 1:1575139/1‑69 (MQ=255) aTCTGGACGATGAACAACTGGCGAAAGTAAGCGCCTTTGCCGATCGTACCATGACGCTGCAGGCAACGa > 1:1829103/1‑69 (MQ=255) aTCTGGACGATGAACAACTGGCGAAAGTAAGCGCCTTTGCCGATCGTACCATGACGCTGCAGGCAACGa > 1:670843/1‑69 (MQ=255) aTCTGGACGATGAACAACTGGCGAAAGTAAGCGCCTTTGCCGATCGTACCATGACGCTGCAGGCAACGa > 1:1247239/1‑69 (MQ=255) aTCTGGACGATGAACAACTGGCGAAAGTAAGCGCCTTTGCCGATCGTACCATGACGCTGCAGGCAACGa > 1:2733746/1‑69 (MQ=255) aTCTGGACGATGAACAACTGGCGAAAGTAAGCGCCTTTGCCGATCGTACCATGACGCTGCAGGCAACGa > 1:1397120/1‑69 (MQ=255) aTCTGGACGATGAACAACTGGCGAAAGTAAGCGCCTTTGCCGATCGTACCATGACGCTGCAGGCAACGa > 1:2854944/1‑69 (MQ=255) aTCTGGACGATGAACAACTGGCGAAAGTAAGCGCCTTTGCCGATCGTACCATGACGCTGCAGGCAACGa > 1:2412294/1‑69 (MQ=255) aTCTGGACGATGAACAACTGGCGAAAGTAAGCGCCTTTGCCGATCGTACCATGACGCTGCAGGCAACGa > 1:2235593/1‑69 (MQ=255) aTCTGGACGATGAACAACTGGCGAAAGTAAGCGCCTTTGCCGATCGTACCATGACGCTGCAGGCAACGa > 1:3067780/1‑69 (MQ=255) aTCTGGACGATGAACAACTGGCGAAAGTAAGCGCCTTTGCCGATCGTACCATGACGCTGCAGGCAACGa > 1:2041051/1‑69 (MQ=255) aTCTGGACGATGAACAACTGGCGAAAGTAAGCGCCTTTGCCGATCGTACCATGACGCTGCAGGCAACGa > 1:3248398/1‑69 (MQ=255) aTCTGGACGATCAACAACTGGCGAAAGTAAGCGCCTTTGCCGATCGTACCATGACGCTGCAGGCAACGa > 1:1739696/1‑69 (MQ=255) tGGACGATGAACAACTGGCGAAAGTAAGCGCCTTTGCCGATCGTACCAt > 1:3370014/1‑49 (MQ=255) tGGACGATGAACAACTGGCGAAAGTAAGCGCCTTTGCCGATCGTACCAt > 1:442698/1‑49 (MQ=255) tGGACGATGAACAACTGGCGAAAGTAAGCGCCTTTGCCGATCGTACCAt > 1:3366171/1‑49 (MQ=255) cGATGAACAACTGGCGATAGTAAGCGCCTTTGCCGATCGTACCATGACGCTGCAGGCAACGATTCAg < 1:761528/67‑1 (MQ=255) cGATGAACAACTGGCGAAAGTTAGCGCCTTTGCCGATCGTACCATGACGCTGCAGGCAACGATTCAg < 1:1058117/67‑1 (MQ=255) cGATGAACAACTGGCGAAAGTAAGCGCCTTTGCCGATCGTACCATGACGCTGCAGGCAACGATTCAg < 1:174677/67‑1 (MQ=255) cGATGAACAACTGGCGAAAGTAAGCGCCTTTGCCGATCGTACCATGACGCTGCAGGCAACGATTCAg < 1:155679/67‑1 (MQ=255) cGATGAACAACTGGCGAAAGTAAGCGCCTTTGCCGATCGTACCATGACGCTGCAGGCAACGATTCAg < 1:3402571/67‑1 (MQ=255) cGATGAACAACTGGCGAAAGTAAGCGCCTTTGCCGATCGTACCATGACGCTGCAGGCAACGATTCAg < 1:3018684/67‑1 (MQ=255) cGATGAACAACTGGCGAAAGTAAGCGCCTTTGCCGATCGTACCATGACGCTGCAGGCAACGATTCAg < 1:1944169/67‑1 (MQ=255) cGATGAACAACTGGCGAAAGTAAGCGCCTTTGCCGATCGTACCATGACGCTGCAGGCAACGATTCAg < 1:2131150/67‑1 (MQ=255) cGATGAACAACTGGCGAAAGTAAGCGCCTTTGCCGATCGTACCATGACGCTGCAGGCAACGATTCAg < 1:1673246/67‑1 (MQ=255) cGATGAACAACTGGCGAAAGTAAGCGCCTTTGCCGATCGTACCATGACGCTGCAGGCAACGATTCAg < 1:1112715/67‑1 (MQ=255) cGATGAACAACTGGCGAAAGTAAGCGCCTTTGCCGATCGTACCATGACGCTGCAGGCAACGATTCAg < 1:101675/67‑1 (MQ=255) cGATGAACAACTGGCGAAAGTAAGCGCCTTTGCCGATCGTACCATGACGCTGCAGGCAACGATTCAg < 1:2412463/67‑1 (MQ=255) cGATGAACAACTGGCGAAAGTAAGCGCCTTTGCCGATCGTACCATGACGCTGCAGGCAACGATTCAg < 1:2422401/67‑1 (MQ=255) cGATGAACAACTGGCGAAAGTAAGCGCCTTTGCCGATCGTACCATGACGCTGCAGGCAACGATTCAg < 1:925189/67‑1 (MQ=255) cGATGAACAACTGGCGAAAGTAAGCGCCTTTGCCGATCGTACCATGACGCTGCAGGCAACGATTCAg < 1:3314727/67‑1 (MQ=255) cGATGAACAACTGGCGAAAGTAAGCGCCTTTGCCGATCGTACAATGACGCTGCAGGCAACGATTCAg < 1:422183/67‑1 (MQ=255) aacaacTGGCGAAAGTAAGCGCCTTTGCCGATCGTACCAt < 1:1902372/40‑1 (MQ=255) aacaacTGGCGAAAGTAAGCGCCTTTGCCGATCGTACCAt < 1:943832/40‑1 (MQ=255) aacaacTGGCGAAAGTAAGCGCCTTTGCCGATCGTACCAt < 1:3127798/40‑1 (MQ=255) aacaacTGGCGAAAGTAAGCGCCTTTGCCGATCGTACCAt < 1:2070891/40‑1 (MQ=255) aacaacTGGCGAAAGTAAGCGCCTTTGCCGATCGTACCAt < 1:942562/40‑1 (MQ=255) caacTGGCGAAAGTAAGCGCCTTTGCCGATCGTACCATGACGCTGCAGGCAACGATTCAGGATGGCGTg < 1:1382722/69‑1 (MQ=255) caacTGGCGAAAGTAAGCGCCTTTGCCGATCGTACCATGACGCTGCAGGCAACGATTCAGGATGGCGTg < 1:536248/69‑1 (MQ=255) caacTGGCGAAAGTAAGCGCCTTTGCCGATCGTACCATGACGCTGCAGGCAACGATTCAGGATGGCGTg < 1:580346/69‑1 (MQ=255) caacTGGCGAAAGTAAGCGCCTTTGCCGATCGTACCATGACGCTGCAGGCAACGATTCAGGATGGCGTg < 1:642431/69‑1 (MQ=255) caacTGGCGAAAGTAAGCGCCTTTGCCGATCGTACCATGACGCTGCAGGCAACGATTCAGGATGGCGTg < 1:1151321/69‑1 (MQ=255) caacTGGCGAAAGTAAGCGCCTTTGCCGATCGTACCATGACGCTGCAGGCAACGATTCAGGATGGCGTg < 1:726942/69‑1 (MQ=255) caacTGGCGAAAGTAAGCGCCTTTGCCGATCGTACCATGACGCTGCAGGCAACGATTCAGGATGGCGTg < 1:2703302/69‑1 (MQ=255) caacTGGCGAAAGTAAGCGCCTTTGCCGATCGTACCATGACGCTGCAGGCAACGATTCAGGATGGCGTg < 1:3337668/69‑1 (MQ=255) caacTGGCGAAAGTAAGCGCCTTTGCCGATCGTACCATGACGCTGCAGGCAACGATTCAGGATGGCGTg < 1:3105751/69‑1 (MQ=255) caacTGGCGAAAGTAAGCGCCTTTGCCGATCGTACCATGACGCTGCAGGCAACGATTCAGGATGGCGTg < 1:311003/69‑1 (MQ=255) caacTGGCGAAAGTAAGCGCCTTTGCCGATCGTACCATGACGCTGCAGGCAACGATTCAGGATGGCGTg < 1:2003810/69‑1 (MQ=255) caacTGGCGAAAGTAAGCGCCTTTGCCGATCGTACCATGACGCTGCAGGCAACGATTCAGGATGGCGTg < 1:2872693/69‑1 (MQ=255) caacTGGCGAAAGTAAGCGCCTTTGCCGATCGTACCATGACGCTGCAGGCAACGATTCAGGATGGCGTg < 1:2158057/69‑1 (MQ=255) aacTGGCGAAAGTAAGCGCCTTTGCCGATCGTACCATGACGCTGCAGGCAACGATTCAGGATGGCGTg < 1:2939773/68‑1 (MQ=255) aacTGGCGAAAGTAAGCGCCTTTGCCGATCGTACCATGACGCTGCAGGCAACGATTCAGGATGGCGTg < 1:577579/68‑1 (MQ=255) aacTGGCGAAAGTAAGCGCCTTTGCCGATCGTACCATGACGCTGCAGGCAACGATTCAGGATGGCGTg < 1:513243/68‑1 (MQ=255) cTGGCGAAAGTGAGCGGCTTTGCCGATCGTACCATGACGCTGCAGGCAACGATTCa > 1:1553783/1‑56 (MQ=255) cTGGCGAAAGTGAGCGGCTTTGCCGATCGTACCATGACGCTGCAGGCAACGATTCAGGATGGTGTTAt > 1:1795310/1‑68 (MQ=255) cTGGCGAAAGTGAGCGGCTTTGCCGATCGTACCATGACGCTGCAGGCAACGATTCAGGATGGTGTTATc > 1:2603917/1‑69 (MQ=255) cTGGCGAAAGTGAGCGGCTTTGCCGATCGTACCATGACGCTGCAGGCAACGATTCAGGATGGTGTTATc > 1:2865937/1‑69 (MQ=255) cTGGCGAAAGTGAGCGGCTTTGCCGATCGTACCATGACGCTGCAGGCAACGATTCAGGATGGTGTTATc > 1:907737/1‑69 (MQ=255) cTGGCGAAAGTGAGCGGCTTTGCCGATCGTACCATGACGCTGCAGGCAACGATTCAGGATGGTGTTATc > 1:1202135/1‑69 (MQ=255) cTGGCGAAAGTGAGCGGCTTTGCCGATCGTACCATGACGCTGCAGGCAACGATTCAGGATGGTGTTATc > 1:2479772/1‑69 (MQ=255) cTGGCGAAAGTGAGCGGCTTTGCCGATCGTACCATGACGCTGCAGGCAACGATTCAGGATGGTGTTATc > 1:2479810/1‑69 (MQ=255) cTGGCGAAAGTGAGCGGCTTTGCCGATCGTACCATGACGCTGCAGGCAACGATTCAGGATGGTGTTATc > 1:1373443/1‑69 (MQ=255) cTGGCGAAAGTGAGCGGCTTTGCCGATCGTACCATGACGCTGCAGGCAACGATTCAGGATGGTGTTATc > 1:496116/1‑69 (MQ=255) cTGGCGAAAGTGAGCGGCTTTGCCGATCGTACCATGACGCTGCAGGCAACGATTCAGGATGGTGTTATc > 1:3140624/1‑69 (MQ=255) cTGGCGAAAGTGAGCGGCTTTGCCGATCGTACCATGACGCTGCAGGCAACGATTCAGGATGGTGTTATc > 1:1671320/1‑69 (MQ=255) cTGGCGAAAGTGAGCGGCTTTGCCGATCGTACCATGACGCTGCAGGCAACGATTCAGGATGGTGTTATc > 1:3158303/1‑69 (MQ=255) cTGGCGAAAGTGAGCGGCTTTGCCGATCGTACCATGACGCTGCAGGCAACGATTCAGGATGGTGTTATc > 1:3363667/1‑69 (MQ=255) cTGGCGAAAGTGAGCGGCTTTGCCGATCGTACCATGACGCTGCAGGCAACGATTCAGGATGGTGTTATc > 1:3356817/1‑69 (MQ=255) cTGGCGAAAGTGAGCGGCTTTGCCGATCGTACAATGACGCTGCAGGCAACGATTCAGGAt > 1:2984291/1‑60 (MQ=255) ggCGAAAGTAAGCGCCTTTGCCGATCGTACCATGACGCTGCAGGCAACGATTCAGGATGGCGTGATCTg > 1:347132/1‑69 (MQ=255) ggCGAAAGTAAGCGCCTTTGCCGATCGTACCATGACGCTGCAGGCAACGATTCAGGATGGCGTGATCTg > 1:3318168/1‑69 (MQ=255) gAAAGTAAGCGCCTTTGCCGATCGTACCATGACGCTGCAGGCAACGATTCAg < 1:2114640/52‑1 (MQ=255) aaGCGCCTTTGCCGATCGTACCATGACGCTGCAGGCAACGATTCAg > 1:40859/1‑46 (MQ=255) | TGCAGTTTGCCAATCTTTCCGTCTGGTATCTGGACGATGAACAACTGGCGAAAGTAAGCGCCTTTGCCGATCGTACCATGACGCTGCAGGCAACGATTCAGGATGGCGTGATCTG > minE/193486‑193600 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |