| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,290,461 | C→G | 38.3% | V391V (GTC→GTG) | ppc → | phosphoenolpyruvate carboxylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,290,461 | 0 | C | G | 38.3% | 74.0 / 99.0 | 107 | V391V (GTC→GTG) | ppc | phosphoenolpyruvate carboxylase |
| Reads supporting (aligned to +/- strand): ref base C (30/36); new base G (9/32); total (39/68) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.24e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.43e-01 | |||||||||||
Only 100 of 108 total aligned reads displayed.
GGTATTATCGCCAACGGCGATCTGCTCGACACCCTGCGCCGCGTGAAATGTTTCGGCGTACCGCTGGTCCGTATTGATATCCGTCAGGAGAGCACGCGTCATACCGAAGCGCTGGGCGAGCTGACCCGCTAC > minE/2290393‑2290524 | ggTATTATCGCCAACGGCGATCTGCTCGACACCCTGCGCCGCGTGAAATGTTTCGGCGTACCGCTGGTc > 1:164028/1‑69 (MQ=255) attatCGCCAACGGCGATCTGCTCGACACCCTGCGCCGCGTGAAATGTTTCGGCGTACCGCTGGTCCGt < 1:69429/69‑1 (MQ=255) attatCGCCAACGGCGATCTGCTCGACACCCTGCGCCGCGTGAAATGTTTCGGCGTACCGCTGGTCCGt < 1:1622309/69‑1 (MQ=255) attatCGCCAACGGCGATCTGCTCGACACCCTGCGCCGCGTGAAATGTTTCGGCGTACCGCTGGTCCGt < 1:1774921/69‑1 (MQ=255) attatCGCCAACGGCGATCTGCTCGACACCCTGCGCCGCGTGAAATGTTTCGGCGTACCGCTGGTCCGt < 1:1776148/69‑1 (MQ=255) atCGCCAACGGCGATCTGCTCGACACCCTGCGCCGCGTGAAATGTTTCGGCGTACCGCTGGTCCg < 1:106457/65‑1 (MQ=255) atCGCCAACGGCGATCTGCTCGACACCCTGCGCCGCGTGAAATGTTTCGGCGTACCGCTGGTCCg < 1:173124/65‑1 (MQ=255) atCGCCAACGGCGATCTGCTCGACACCCTGCGCCGCGTGAAATGTTTCGGCGTACCGCTGGTCCg < 1:1186901/65‑1 (MQ=255) atCGCCAACGGCGATCTGCTCGACACCCTGCGCCGCGTGAAATGTTTCGGCGTACCGCTGGTCCg < 1:2991758/65‑1 (MQ=255) atCGCCAACGGCGATCTGCTCGACACCCTGCGCCGCGTGAAATGTTTCGGCGTACCGCTGGTCCg < 1:3159528/65‑1 (MQ=255) atCGCCAACGGCGATCTGCTCGACACCCTGCGCCGCGTGAAATGTTTCGGCGTACCGCTGGTCCg < 1:730727/65‑1 (MQ=255) atCGCCAACGGCGATCTGCTCGACACCCTGCGCCGCGTGAAATGTTTCGGCGTACCGCTGGTCCGTAtt < 1:649307/69‑1 (MQ=255) atCGCCAACGGCGATCTGCTCGACACCCTGCGCCGCGTGAAATGTTTCGGCGTACCGCTGGTCCGTAtt > 1:989356/1‑69 (MQ=255) atCGCCAACGGCGATCTGCTCGACACCCTGCGCCGCGTGAAATGTTTCGGCGTACCGCTGGTCCGTAtt > 1:1786616/1‑69 (MQ=255) atCGCCAACGGCGATCTGCTCGACACCCTGCGCCGCGTGAAATGTTTCGGCGTACCGCTGGTCCGTAtt > 1:1798805/1‑69 (MQ=255) atCGCCAACGGCGATCTGCTCGACACCCTGCGCCGCGTGAAATGTTTCGGCGTACCGCTGGTCCGTAtt > 1:1805937/1‑69 (MQ=255) atCGCCAACGGCGATCTGCTCGACACCCTGCGCCGCGTGAAATGTTTCGGCGTACCGCTGGTCCGTAtt > 1:1838118/1‑69 (MQ=255) atCGCCAACGGCGATCTGCTCGACACCCTGCGCCGCGTGAAATGTTTCGGCGTACCGCTGGTCCGTAtt > 1:508758/1‑69 (MQ=255) atCGCCAACGGCGATCTGCTCGACACCCTGCGCCGCGTGAAATGTTTCGGCGTACCGCTGGTCCGTAtt < 1:2175015/69‑1 (MQ=255) atCGCCAACGGCGATCTGCTCGACACCCTGCGCCGCGTGAAATGTTTCGGCGTACCGCTGGTCCGTAtt > 1:2176259/1‑69 (MQ=255) atCGCCAACGGCGATCTGCTCGACACCCTGCGCCGCGTGAAATGTTTCGGCGTACCGCTGGTCCGTAtt < 1:2269924/69‑1 (MQ=255) atCGCCAACGGCGATCTGCTCGACACCCTGCGCCGCGTGAAATGTTTCGGCGTACCGCTGGTCCGTAtt > 1:231519/1‑69 (MQ=255) atCGCCAACGGCGATCTGCTCGACACCCTGCGCCGCGTGAAATGTTTCGGCGTACCGCTGGTCCGTAtt > 1:2501305/1‑69 (MQ=255) atCGCCAACGGCGATCTGCTCGACACCCTGCGCCGCGTGAAATGTTTCGGCGTACCGCTGGTCCGTAtt > 1:3433812/1‑69 (MQ=255) atCGCCAACGGCGATCTGCTCGACACCCTGCGCCGCGTGAAATGTTTCGGCGTACCGCTGGTCCGTAtt > 1:3423916/1‑69 (MQ=255) atCGCCAACGGCGATCTGCTCGACACCCTGCGCCGCGTGAAATGTTTCGGCGTACCGCTGGTCCGTAtt > 1:146517/1‑69 (MQ=255) atCGCCAACGGCGATCTGCTCGACACCCTGCGCCGCGTGAAATGTTTCGGCGTACCGCTGGTCCGTAtt > 1:3185085/1‑69 (MQ=255) atCGCCAACGGCGATCTGCTCGACACCCTGCGCCGCGTGAAATGTTTCGGCGTACCGCTGGTCCGTAtt > 1:3353668/1‑69 (MQ=255) atCGCCAACGGCGATCTGCTCGACACCCTGCGCCGCGTGAAATGTTTCGGCGTACCGCTGGTCCGTAtt < 1:1140495/69‑1 (MQ=255) atCGCCAACGGCGATCTGCTCGACACCCTGCGCCGCGTGAAATGTTTCGGCGTACCGCTGGTCCGTAtt > 1:1343474/1‑69 (MQ=255) atCGCCAACGGCGATCTGCTCGACACCCTGCGCCGCGTGAAATGTTTCGGCGTACCGCTGGTCCGTAtt > 1:1346762/1‑69 (MQ=255) atCGCCAACGGCGATCTGCTCGACACCCTGCGCCGCGTGAAATGTTTCGGCGTACCGCTGGTCCGTAtt < 1:1470281/69‑1 (MQ=255) atCGCCAACGGCGATCTGCTCGACACCCTGCGCCGCGTGAAATGTTTCGGCGTACCGCTGGTCCGTAtt > 1:1128115/1‑69 (MQ=255) tCGCCAACGGCGATCTGCTCGACACCCTGCGCCGCGTGAAATGTTTCGGCGTACCGCTGGTCCGTAtt < 1:1984024/68‑1 (MQ=255) gCCAACGGCGATCTGCTCGCCACCCTGCGCCGCGTGAAATGTTTCGGCGTACCGCTGGTCCGTATTGat > 1:2190243/1‑69 (MQ=255) gCCAACGGCGATCTGCTCGACACCCTGCGCCGCGTGAAATGTTTCGGCGTACCGCTGGTCCGTATTGat < 1:505958/69‑1 (MQ=255) gCCAACGGCGATCTGCTCGACACCCTGCGCCGCGTGAAATGTTTCGGCGTACCGCTGGTCCGTATTGat < 1:79725/69‑1 (MQ=255) gCCAACGGCGATCTGCTCGACACCCTGCGCCGCGTGAAATGTTTCGGCGTACCGCTGGTCCGTATTGat < 1:2567116/69‑1 (MQ=255) gCCAACGGCGATCTGCTCGACACCCTGCGCCGCGTGAAATGTTTCGGCGTACCGCTGGTCCGTATTGat < 1:2320824/69‑1 (MQ=255) gCCAACGGCGATCTGCTCGACACCCTGCGCCGCGTGAAATGTTTCGGCGTACCGCTGGTCCGTATTGat > 1:474578/1‑69 (MQ=255) gCCAACGGCGATCTGCTCGACACCCTGCGCCGCGTGAAATGTTTCGGCGTACCGCTGGTCCGTATTGat < 1:3326995/69‑1 (MQ=255) gCCAACGGCGATCTGCTCGACACCCTGCGCCGCGTGAAATGTTTCGGCGTACCGCTGGTCCGTATTGat < 1:2829427/69‑1 (MQ=255) gCCAACGGCGATCTGCTCGACACCCTGCGCCGCGTGAAATGTTTCGGCGTACCGCTGGTCCGTATTGat > 1:763464/1‑69 (MQ=255) gCCAACGGCGATCTGCTCGACACCCTGCGCCGCGTGAAATGTTTCGGCGTACCGCTGGTCCGTATTGat > 1:2118758/1‑69 (MQ=255) gCCAACGGCGATCTGCTCGACACCCTGCGCCGCGTGAAATGTTTCGGCGTACCGCTGGTCCGTATTGat > 1:3375584/1‑69 (MQ=255) gCCAACGGCGATCTGCTCGACACCCTGCGCCGCGTGAAATGTTTCGGCGTACCGCTGGTCCGTATTGat > 1:2160287/1‑69 (MQ=255) gCCAACGGCGATCTGCTCGACACCCTGCGCCGCGTGAAATGTTTCGGCGTACCGCTGGTCCGTATTGat < 1:630021/69‑1 (MQ=255) gCCAACGGCGATCTGCTCGACACCCTGCGCCGCGTGAAATGTTTCGGCGTACCGCTGGTCCGTATTGat > 1:1492230/1‑69 (MQ=255) gCCAACGGCGATCTGCTCGACACCCTGCGCCGCGTGAAATGTTTCGGCGTACCGCTGGTCCGTATTGat > 1:1770725/1‑69 (MQ=255) gCCAACGGCGATCTGATCGACACCCTGCGCCGCGTGAAATGTTTCGGCGTACCGCTGGTCCGTATTGa > 1:1911167/1‑68 (MQ=255) cGATCTGCTCGACACCCTGCGCCGCGTGAAATGTTTCGGCGTACCGCTGGTGCGTATTGATATCCGt > 1:618043/1‑67 (MQ=255) cGATCTGCTCGACACCCTGCGCCGCGTGAAATGTTTCGGCGTACCGCTGGTGCGTATTGATATCCGTc > 1:2432390/1‑68 (MQ=255) cGATCTGCTCGACACCCTGCGCCGCGTGAAATGTTTCGGCGTACCGCTGGTGCGTATTGATATCCGTc > 1:697409/1‑68 (MQ=255) cGATCTGCTCGACACCCTGCGCCGCGTGAAATGTTTCGGCGTACCGCTGGTGCGTATTGATATCCGTc > 1:3392993/1‑68 (MQ=255) cGATCTGCTCGACACCCTGCGCCGCGTGAAATGTTTCGGCGTACCGCTGGTGCGTATTGATATCCGTc > 1:287103/1‑68 (MQ=255) cGATCTGCTCGACACCCTGCGCCGCGTGAAATGTTTCGGCGTACCGCTGGTGCGTATTGATATCCGTc > 1:1603296/1‑68 (MQ=255) cGATCTGCTCGACACCCTGCGCCGCGTGAAATGTTTCGGCGTACCGCTGGTGCGTATTGATATCCGTc > 1:2470284/1‑68 (MQ=255) cGATCTGCTCGACACCCTGCGCCGCGTGAAATGTTTCGGCGTACCGCTGGTGCGTATTGATATCCGTc > 1:2325835/1‑68 (MQ=255) cGATCTGCTCGACACCCTGCGCCGCGTGAAATGTTTCGGCGTACCGCTGGTGCGTATTGATATCCGTc > 1:1964981/1‑68 (MQ=255) cTGCTCGACACCCTGCGCCGCGTGAAATGTTTCGGCGTACCGCTGGTCCGTATTGATATCCGTCAGgag > 1:1659643/1‑69 (MQ=255) acCCTGCGCCGCGTGAAATGTTTCGGCGTACCGCTGGTCCGTATTGATATCCGTCAGGAGAGCACGCGt < 1:3240205/69‑1 (MQ=255) acCCTGCGCCGCGTGAAATGTTTCGGCGTACCGCTGGTCCGTATTGATATCCGTCAGGAGAGCACGCGt < 1:343111/69‑1 (MQ=255) acCCTGCGCCGCGTGAAATGTTTCGGCGTACCGCTGGTCCGTATTGATATCCGTCAGGAGAGCACGCGt < 1:1815728/69‑1 (MQ=255) acCCTGCGCCGCGTGAAATGTTTCGGCGTACCGCTGGTCCGTATTGATATCCGTCAGGAGAGCACGCGt < 1:1490341/69‑1 (MQ=255) acCCTGCGCCGCGTGAAATGTTTCGGCGTACCGCTGGTCCGTATTGATATCCGTCAGGAGAGCACGCGt < 1:119493/69‑1 (MQ=255) acCCTGCGCCGCGTGAAATGTTTCGGCGTACCGCTGGTCCGTATTGATATCCGTCAGGAGAGCACGCGt < 1:965475/69‑1 (MQ=255) acCCTGCGCCGCGTGAAATGTTTCGGCGTACCGCTGGTCCGTATTGATATCCATCAGGAGAGCACGCGt < 1:804004/69‑1 (MQ=255) ccgtGCGCCGCGTGAAATGTTTCGGCGTACCGCTGGTGCGTATTGATATCCGTCAGGAGAGCACGCGTc < 1:1428081/66‑1 (MQ=255) cccTGCGCCGCGTGAAATGTTTCGGCGTACCGCTGGTGCGTATTGATATCCGTCAGGAGAGCACGCGTc < 1:2351743/69‑1 (MQ=255) cccTGCGCCGCGTGAAATGTTTCGGCGTACCGCTGGTGCGTATTGATATCCGTCAGGAGAGCACGCGTc < 1:1644448/69‑1 (MQ=255) cccTGCGCCGCGTGAAATGTTTCGGCGTACCGCTGGTGCGTATTGATATCCGTCAGGAGAGCACGCGTc < 1:2142139/69‑1 (MQ=255) cccTGCGCCGCGTGAAATGTTTCGGCGTACCGCTGGTGCGTATTGATATCCGTCAGGAGAGCACGCGTc < 1:1115879/69‑1 (MQ=255) cccTGCGCCGCGTGAAATGTTTCGGCGTACCGCTGGTGCGTATTGATATCCGTCAGGAGAGCACGCGTc < 1:1179493/69‑1 (MQ=255) cccTGCGCCGCGTGAAATGTTTCGGCGTACCGCTGGTGCGTATTGATATCCGTCAGGAGAGCACGCGTc < 1:26071/69‑1 (MQ=255) cccTGCGCCGCGTGAAATGTTTCGGCGTACCGCTGGTGCGTATTGATATCCGTCAGGAGAGCACGCGTc < 1:1447020/69‑1 (MQ=255) cccTGCGCCGCGTGAAATGTTTCGGCGTACCGCTGGTGCGTATTGATATCCGTCAGGAGAGCACGCGTc < 1:1282370/69‑1 (MQ=255) cccTGCGCCGCGTGAAATGTTTCGGCGTACCGCTGGTGCGTATTGATATCCGTCAGGAGAGCACGCGTc < 1:273901/69‑1 (MQ=255) cccTGCACCGCGTGAAATGTTTCGGCGTACCGCTGGTGCGTATTGATATCCGTCAGGAGAGCACGCGTc < 1:1745651/69‑1 (MQ=255) ccTGCGCCGCGTGAAATGTTTCGGCGTACCGCTGGTGCGTATTGATATCCGTCAGGAGAGCACGCGTc < 1:2824525/68‑1 (MQ=255) cTGCGCCGCGTGAAATGTTTCGGCGTACCGCTGGTCCGTATTGATATCCGTCAGGAGAGCACGCGTCAt > 1:3270125/1‑69 (MQ=255) tGCGCCGCGTGAAATGTTTCGGCGTACCGCTGGTGCGTATTGATATCCGTCAGTagag < 1:3298236/58‑1 (MQ=255) tGCGCCGCGTGAAATGTTTCGGCGTACCGCTGGTGCGTATTGATATCCGTCAGGagag < 1:768305/58‑1 (MQ=255) tGCGCCGCGTGAAATGTTTCGGCGTACCGCTGGTGCGTATTGATATCCGTCAGGagag < 1:786084/58‑1 (MQ=255) tGCGCCGCGTGAAATGTTTCGGCGTACCGCTGGTGCGTATTGATATCCGTCAGGagag < 1:1203084/58‑1 (MQ=255) tGCGCCGCGTGAAATGTTTCGGCGTACCGCTGGTGCGTATTGATATCCGTCAGGagag < 1:1638834/58‑1 (MQ=255) tGCGCCGCGTGAAATGTTTCGGCGTACCGCTGGTGCGTATTGATATCCGTCAGGagag < 1:1025945/58‑1 (MQ=255) tGCGCCGCGTGAAATGTTTCGGCGTACCGCTGGTGCGTATTGATATCCGTCAGGagag < 1:1704722/58‑1 (MQ=255) tGCGCCGCGTGAAATGTTTCGGCGTACCGCTGGTGCGTATTGATATCCGTCAGGagag < 1:510825/58‑1 (MQ=255) tGCGCCGCGTGAAATGTTTCGGCGTACCGCTGGTGCGTATTGATATCCGTCAGGagag < 1:552810/58‑1 (MQ=255) tGCGCCGCGTGAAATGTTTCGGCGTACCGCTGGTGCGTATTGATATCCGTCAGGagag < 1:1007524/58‑1 (MQ=255) tGCGCCGCGTGAAATGTTTCGGCGTACCGCTGGTGCGTATTGATATCCGTCAGGagag < 1:2613888/58‑1 (MQ=255) tGCGCCGCGTGAAATGTTTCGGCGTACCGCTGGTGCGTATTGATATCCGTCAGGagag < 1:3398435/58‑1 (MQ=255) tGCGCCGCGTGAAATGTTTCGGCGTACCGCTGGTGCGTATTGATATCCGTCAGGagag < 1:3098044/58‑1 (MQ=255) tGCGCCGCGTGAAATGTTTCGGCGTACCGCTGGTGCGTATTGATATCCGTCAGGagag < 1:3348865/58‑1 (MQ=255) tGCGCCGCGTGAAATGTTTCGGCGTACCGCTGGTGCGTATTGATATCCGTCAGGagag < 1:3192800/58‑1 (MQ=255) tGCGCCGCGTGAAATGTTTCGGCGTACCGCTGGTGCGTATTGATATCCGTCAGGagag < 1:3275576/58‑1 (MQ=255) cGTACCGCTGGTGCGTATTGATATCCGTCAGGagag < 1:2730137/36‑1 (MQ=255) aCCGCTGGTGCGTATTGATATCCGTCAGGAGAGCACGCGTc < 1:1873683/41‑1 (MQ=255) cTGGTCCGTATTGATATCCGTCAGGAGAGCACGCGTCATACCGAAGCGCTGGGCGAGCTGACCCGCTAc < 1:553907/69‑1 (MQ=255) cTGGTCCGTATTGATATCCGTCAGGAGAGCACGCGTCATACCGAAGCGCTGGGCGAGCTGACCCGCTAc < 1:3185093/69‑1 (MQ=255) | GGTATTATCGCCAACGGCGATCTGCTCGACACCCTGCGCCGCGTGAAATGTTTCGGCGTACCGCTGGTCCGTATTGATATCCGTCAGGAGAGCACGCGTCATACCGAAGCGCTGGGCGAGCTGACCCGCTAC > minE/2290393‑2290524 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |