| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,290,614 | G→A | 54.6% | P442P (CCG→CCA) | ppc → | phosphoenolpyruvate carboxylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,290,614 | 0 | G | A | 54.6% | 5.0 / 43.6 | 55 | P442P (CCG→CCA) | ppc | phosphoenolpyruvate carboxylase |
| Reads supporting (aligned to +/- strand): ref base G (6/19); new base A (22/8); total (28/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.90e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AAACAGGCGTTCCTGATCCGCGAACTGAACTCCAAACGTCCGCTTCTGCCGCGCAACTGGCAACCAAGCGCCGAAACGCGCGAAGTGCTCGATACCTGCCAGGTGATTGCCGAAGC > minE/2290564‑2290679 | aaaCAGGCGTTCCTGATCCGCGAACTGAACTCCAAACGTCCGCTTCTGCCGCGCAACTGGCAACCAAgc > 1:202168/1‑69 (MQ=255)aaaCAGGCGTTCCTGATCCGCGAACTGAACTCCAAACGTCCGCTTCTGCCGCGCAACTGGCAACCAAgc > 1:1415531/1‑69 (MQ=255) aCAGGCGTTCCTGATCCGCGAACTGAACTCCAAACGTCCGCTTCTGCCGCGCAACTGGCAACCAAgcg > 1:2392992/1‑68 (MQ=255) aCAGGCGTTCCTGATCCGCGAACTGAACTCCAAACGTCCGCTTCTGCCGCGCAACTGGCAACCAAgcg > 1:1543282/1‑68 (MQ=255) cAGGCGTTCCTGATCCGCGAACTGAACTCCAAACGTCCGCTTCTACCACGCAACTGGCAACGAAGCGcc < 1:3103149/69‑1 (MQ=255) cAGGCGTTCCTGATCCGCGAACTGAACTCCAAACGTCCGCTTCTACCACGCAACTGGCAACCAAGCGcc < 1:1218655/69‑1 (MQ=255) cAGGCGTTCCTGATCCGCGAACTGAACTCCAAACGTCCGCTTCTACCACGCAACTGGCAACCAAGCGcc < 1:1890102/69‑1 (MQ=255) cAGGCGTTCCTGATCCGCGAACTGAACTCCAAACGTCCGCTTCTACCACGCAACTGGCAACCAAGCGcc < 1:1706367/69‑1 (MQ=255) cAGGCGTTCCTGATCCGCGAACTGAACTCCAAACGTCCGCTTCTACCACGCAACTGGCAACCAAGCGcc < 1:3308789/69‑1 (MQ=255) cAGGCGTTCCTGATCCGCGAACTGAACTCCAAACGTCCGCTTCTACCACGCAACTGGCAACCAAGCGcc < 1:526992/69‑1 (MQ=255) cAGGCGTTCCTGATCCGCGAACTGAACTCCAAACGTCCGCTTCTACCACGCAACTGGCAACCAAGCGcc < 1:737453/69‑1 (MQ=255) cAGGCGTTCCTGATCCGCGAACTGAACTCCAAACGTCCGCTTCTACCACGCAACTGGCAACCAAGCGcc < 1:997602/69‑1 (MQ=255) ccTGATCCGCGAACTGAACTCCAAACGTCCGCTTCTGCCGCGCAACTGGCAACCAAGCGCCGAAAcgc > 1:152723/1‑68 (MQ=255) ccTGATCCGCGAACTGAACTCCAAACGTCCGCTTCTGCCGCGCAACTGGCAACCAAGCGCCGAAAcgc > 1:579750/1‑68 (MQ=255) gaactgaactCCAAACGTCCGCTTCTGCCGCGCAACTGGCAACCAAGCGCCGAAACGCGCGAAGTGCTc < 1:3298691/69‑1 (MQ=255) gaactgaactCCAAACGTCCGCTTCTGCCGCGCAACTGGCAACCAAGCGCCGAAACGCGCGAAGTGCTc < 1:598417/69‑1 (MQ=255) gaactgaactCCAAACGTCCGCTTCTGCCGCGCAACTGGCAACCAAGCGCCGAAACGCGCGAAGTGCTc < 1:2984537/69‑1 (MQ=255) gaactgaactCCAAACGTCCGCTTCTGCCGCGCAACTGGCAACCAAGCGCCGAAACGCGCGAAGTGCTc < 1:2962175/69‑1 (MQ=255) gaactgaactCCAAACGTCCGCTTCTGCCGCGCAACTGGCAACCAAGCGCCGAAACGCGCGAAGTGCTc < 1:1232411/69‑1 (MQ=255) gaactgaactCCAAACGTCCGCTTCTGCCGCGCAACTGGCAACCAAGCGCCGAAACGCGCGAAGTGCTc < 1:2875254/69‑1 (MQ=255) gaactgaactCCAAACGTCCGCTTCTGCCGCGCAACTGGCAACCAAGCGCCGAAACGCGCGAAGTGCTc < 1:2711120/69‑1 (MQ=255) gaactgaactCCAAACGTCCGCTTCTGCCGCGCAACTGGCAACCAAGCGCCGAAACGCGCGAAGTGCTc < 1:2413532/69‑1 (MQ=255) gaactgaactCCAAACGTCCGCTTCTGCCGCGCAACTGGCAACCAAGCGCCGAAACGCGCGAAGTGCTc < 1:2603784/69‑1 (MQ=255) ctgaactCCAAACGTCCGCTTCTGCCGCGCAACTGGCAACCAAGCGCCGAAACGCGCGAAGTGCTCGAt < 1:215277/69‑1 (MQ=255) ctgaactCCAAACGTCCGCTTCTGCCGCGCAACTGGCAACCAAGCGCCGAAACGCGCGAAGTGCTCGAt < 1:3328730/69‑1 (MQ=255) ctCCAAACGTCCGCTTCTACCACGCAACTGGCAACCAAGCGCCGAAACGCGCGAAGTGCTCGATACCt > 1:1493032/1‑68 (MQ=255) ctCCAAACGTCCGCTTCTACCACGCAACTGGCAACCAAGCGCCGAAACGCGCGAAGTGCTCGATACCt > 1:1358087/1‑68 (MQ=255) ctCCAAACGTCCGCTTCTACCACGCAACTGGCAACCAAGCGCCGAAACGCGCGAAGTGCTCGATACCt > 1:947325/1‑68 (MQ=255) ctCCAAACGTCCGCTTCTACCACGCAACTGGCAACCAAGCGCCGAAACGCGCGAAGTGCTCGATACCt > 1:883312/1‑68 (MQ=255) ctCCAAACGTCCGCTTCTACCACGCAACTGGCAACCAAGCGCCGAAACGCGCGAAGTGCTCGATACCt > 1:864300/1‑68 (MQ=255) ctCCAAACGTCCGCTTCTACCACGCAACTGGCAACCAAGCGCCGAAACGCGCGAAGTGCTCGATACCt > 1:862442/1‑68 (MQ=255) ctCCAAACGTCCGCTTCTACCACGCAACTGGCAACCAAGCGCCGAAACGCGCGAAGTGCTCGATACCt > 1:754697/1‑68 (MQ=255) ctCCAAACGTCCGCTTCTACCACGCAACTGGCAACCAAGCGCCGAAACGCGCGAAGTGCTCGATACCt > 1:1369211/1‑68 (MQ=255) ctCCAAACGTCCGCTTCTACCACGCAACTGGCAACCAAGCGCCGAAACGCGCGAAGTGCTCGATACCt > 1:674116/1‑68 (MQ=255) ctCCAAACGTCCGCTTCTACCACGCAACTGGCAACCAAGCGCCGAAACGCGCGAAGTGCTCGATACCt > 1:1396331/1‑68 (MQ=255) ctCCAAACGTCCGCTTCTACCACGCAACTGGCAACCAAGCGCCGAAACGCGCGAAGTGCTCGATACCt > 1:396917/1‑68 (MQ=255) ctCCAAACGTCCGCTTCTACCACGCAACTGGCAACCAAGCGCCGAAACGCGCGAAGTGCTCGATACCt > 1:3387916/1‑68 (MQ=255) ctCCAAACGTCCGCTTCTACCACGCAACTGGCAACCAAGCGCCGAAACGCGCGAAGTGCTCGATACCt > 1:2415583/1‑68 (MQ=255) ctCCAAACGTCCGCTTCTACCACGCAACTGGCAACCAAGCGCCGAAACGCGCGAAGTGCTCGATACCt > 1:2387202/1‑68 (MQ=255) ctCCAAACGTCCGCTTCTACCACGCAACTGGCAACCAAGCGCCGAAACGCGCGAAGTGCTCGATACCt > 1:3162365/1‑68 (MQ=255) ctCCAAACGTCCGCTTCTACCACGCAACTGGCAACCAAGCGCCGAAACGCGCGAAGTGCTCGATACCt > 1:3171644/1‑68 (MQ=255) ctCCAAACGTCCGCTTCTACCACGCAACTGGCAACCAAGCGCCGAAACGCGCGAAGTGCTCGATACCt > 1:3229781/1‑68 (MQ=255) ctCCAAACGTCCGCTTCTACCACGCAACTGGCAACCAAGCGCCGAAACGCGCGAAGTGCTCGATACCt > 1:151401/1‑68 (MQ=255) ctCCAAACGTCCGCTTCTACCACGCAACTGGCAACCAAGCGCCGAAACGCGCGAAGTGCTCGATACCt > 1:3338289/1‑68 (MQ=255) ctCCAAACGTCCGCTTCTACCACGCAACTGGCAACCAAGCGCCGAAACGCGCGAAGTGCTCGATACCt > 1:344828/1‑68 (MQ=255) ctCCAAACGTCCGCTTCTACCACGCAACTGGCAACCAAGCGCCGAAACGCGCGAAGTGCTAGATACCt > 1:593246/1‑68 (MQ=255) ctCCAAACGTCCGCTTCT‑ACACGCAACTGGCAACCAAGCGCCGAAACGCGCGAAGTGCTCGATACCt > 1:86565/1‑67 (MQ=255) cGTCCGCTTCTGCCGCGCAACTGGCAACCAAGCGCCGAAACGCGCGAAGTGCTCGATACCTGCCAGGTg < 1:556148/69‑1 (MQ=255) cGTCCGCTTCTGCCGCGCAACTGGCAACCAAGCGCCGAAACGCGCGAAGTGCTCGATACCTGCCAGGTg < 1:946624/69‑1 (MQ=255) cGTCCGCTTCTGCCGCGCAACTGGCAACCAAGCGCCGAAACGCGCGAAGTGCTCGATACCTGCCAGGTg < 1:2830658/69‑1 (MQ=255) cGTCCGCTTCTGCCGCGCAACTGGCAACCAAGCGCCGAAACGCGCGAAGTGCTCGATACCTGCCAGGTg < 1:976137/69‑1 (MQ=255) gTCCGCTTCTGCCGCGCAACTGGCAACCAAGCGCCGAAACGCGCGAAGTGCTCGATACCTGCCAGGTg < 1:778832/68‑1 (MQ=255) gTCCGCTTCTGCCGCGCAACTGGCAACCAAGCGCCGAAACGCGCGAAGTGCTCGATACCTGCCAGGTg < 1:1763916/68‑1 (MQ=255) ccGCTTCTGCCGCGCAACTGGCAACCAAGCGCCGAAACGCGCGAAGTGCTc < 1:1700074/51‑1 (MQ=255) ccGCGCAACTGGCAACCAAGCGCCGAAACGCGCGAAGTGCTCGATACCTGCCAGGTGATTGCCGAAGc < 1:1392793/68‑1 (MQ=255) gcgcAACTGGCAACCAAGCGCCGAAACGCGCGAAGTGCTCGATACCTGCCAGGTGATTGCCGAAGc < 1:839750/66‑1 (MQ=255) | AAACAGGCGTTCCTGATCCGCGAACTGAACTCCAAACGTCCGCTTCTGCCGCGCAACTGGCAACCAAGCGCCGAAACGCGCGAAGTGCTCGATACCTGCCAGGTGATTGCCGAAGC > minE/2290564‑2290679 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |