| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,290,935 | A→C | 20.0% | G549G (GGA→GGC) | ppc → | phosphoenolpyruvate carboxylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,290,935 | 0 | A | C | 20.0% | 138.1 / 33.6 | 85 | G549G (GGA→GGC) | ppc | phosphoenolpyruvate carboxylase |
| Reads supporting (aligned to +/- strand): ref base A (30/38); new base C (2/15); total (32/53) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.30e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.48e-01 | |||||||||||
TCGTGGCCTGATTCAGGGCAAACAGATGGTGATGATTGGCTATTCCGACTCAGC‑AAAAGATGCGGGAGTGATGGCAGCTTCCTGGGCGCAATATCAGGCACAGGATGCATTAATCAAAACCTGCGAAAAAGCGGG > minE/2290869‑2291003 | tCGTGGCCTGATTCAGGGCAATCAGATGGTGATGATTGGCTATTCCGACTCAGC‑AAAAGATGCGGGAGt < 1:630629/69‑1 (MQ=255)tCGTGGCCTGATTCAGGGCAAACAGATGGTGATGATTGGCTATTCCGACTCAGC‑AAAAGATGCGGGAGt < 1:1058671/69‑1 (MQ=255)tCGTGGCCTGATTCAGGGCAAACAGATGGTGATGATTGGCTATTCCGACTCAGC‑AAAAGATGCGGGAGt < 1:2113569/69‑1 (MQ=255)tCGTGGCCTGATTCAGGGCAAACAGATGGTGATGATTGGCTATTCCGACTCAGC‑AAAAGATGCGGGAGt < 1:2452272/69‑1 (MQ=255)tCGTGGCCTGATTCAGGGCAAACAGATGGTGATGATTGGCTATTCCGACTCAGC‑AAAAGATGCGGGAGt < 1:2961514/69‑1 (MQ=255)tCGTGGCCTGATTCAGGGCAAACAGATGGTGATGATTGGCTATTCCGACTCAGC‑AAAAGATGCGGGAGt < 1:693089/69‑1 (MQ=255)tCGTGGCCTGATTCAGGGCAAACAGATGGTGATGATTGGCTATTCCGACTCAGC‑AAAAGATGCGGGAGt < 1:1234691/69‑1 (MQ=255)tCGTGGCCTGATTCAGGGCAAACAGATGGTGATGATTGGCTATTCCGACTCAGC‑AAAAGATGCGGGAGt < 1:2859504/69‑1 (MQ=255)tCGTGGCCTGATTCAGGGCAAACAGATGGTGATGATTGGCTATTCCGACTCAGC‑AAAAGATGCGGGAGt < 1:3255397/69‑1 (MQ=255)tCGTGGCCTGATTCAGGGCAAACAGATGGTGATGATTGGCTATTCCGACTCAGC‑AAAAGATGCGGGAGt > 1:1933733/1‑69 (MQ=255)tCGTGGCCTGATTCAGGGCAAACAGATGGTGATGATTGGCTATTCCGACTCAGC‑AAAAGATGCGGGAGt > 1:3339698/1‑69 (MQ=255)tCGTGGCCTGATTCAGGGCAAACAGATGGTGATGATTGGCTATTCCGACTCAGC‑AAAAGATGCGGGAGt < 1:3413625/69‑1 (MQ=255) gTGGCCTTATTCAGGGCAAACAGATGGTGATGATTGGCTATTCCGACTCAGC‑AAAAGATGCGGGAGTg < 1:488932/68‑1 (MQ=255) gTGGCCTGATTCAGGGCAAACAGATGGTGATGATTGGCTATTCCGACTCAGC‑AAAAGATGCGGGAGTg < 1:2914875/68‑1 (MQ=255) gTGGCCTGATTCAGGGCAAACAGATGGTGATGATTGGCTATTCCGACTCAGC‑AAAAGATGCGGGAGTg < 1:1590965/68‑1 (MQ=255) gTGGCCTGATTCAGGGCAAACAGATGGTGATGATTGGCTATTCCGACTCAGC‑AAAAGATGCGGGAGTg < 1:1573422/68‑1 (MQ=255) gTGGCCTGATTCAGGGCAAACAGATGGTGATGATTGGCTATTCCGACTCAGC‑AAAAGATGCGGGAGTGa > 1:1491142/1‑69 (MQ=255) gTGGCCTGATTCAGGGCAAACAGATGGTGATGATTGGCTATTCCGACTCAGC‑AAAAGATGCGGGAGTGa > 1:1616263/1‑69 (MQ=255) gTGGCCTGATTCAGGGCAAACAGATGGTGATGATTGGCTATTCCGACTCAGC‑AAAAGATGCGGGAGTGa > 1:1201861/1‑69 (MQ=255) gTGGCCTGATTCAGGGCAAACAGATGGTGATGATTGGCTATTCCGACTCAGC‑AAAAGATGCGGGAGTGa > 1:1710608/1‑69 (MQ=255) gTGGCCTGATTAAGGGCAAACAGATGGTGATGATTGGCTATTCCGACTCAGC‑AAAAGATGCGGGAGTGa > 1:2455581/1‑69 (MQ=255) tCAGGGCAAACAGATGGTGATGATTGGCTATTCCGACTGAGC‑AAAAGATGCGGGCGTGATGGCAGCTTc > 1:2893279/1‑69 (MQ=255) tCAGGGCAAACAGATGGTGATGATTGGCTATTCCGACTCAGC‑AAAAGATGCGGGCGTGATGGCAGCTTc > 1:1357342/1‑69 (MQ=255) aaCAGATGGTGATGATTGGCTATTCCGACTCAGC‑AAAAGATGCGGGAGTGATGGCAGCTTCCTGGgcgc > 1:2776545/1‑69 (MQ=255) cAGATGGTGATGATTGGCTATTCCGACTCAGC‑AAAAGATGCGGGAGTGATGGCAGCTTCCTGGGCGCaa > 1:2850923/1‑69 (MQ=255) cAGATGGTGATGATTGGCTATTCCGACTCAGC‑AAAAGATGCGGGAGTGATGGCAGCTTCCTGGGCGCaa > 1:1300005/1‑69 (MQ=255) cAGATGGTGATGATTGGCTATTCCGACTCAGC‑AAAAGATGCGGGAGTGATGGCAGCTTCCTGGGCGCa > 1:1735414/1‑68 (MQ=255) cAGATGGTGATGATTGGCTATTCCGACTCAGC‑AAAAGATGCGGGAGTGATGGCAGCTTCCTGGGCGCa > 1:2973762/1‑68 (MQ=255) atTGGCTATTCCGACTCAGC‑AAAAGATGCGGGAGTGATGGCAGCTTc > 1:3152908/1‑47 (MQ=255) atTGGCTATTCCGACTCAGC‑AAAAGATGCGGGAGTGATGGCAGCTTCCTGGGCGCAATATCAGGcaca > 1:2267085/1‑68 (MQ=255) atTGGCTATTCCGACTCAGC‑AAAAGATGCGGGAGTGATGGCAGCTTCCTGGGCGCAATATCAGGCACAg > 1:1578803/1‑69 (MQ=255) atTGGCTATTCCGACTCAGC‑AAAAGATGCGGGAGTGATGGCAGCTTCCTGGGCGCAATATCAGGCACAg < 1:1759328/69‑1 (MQ=255) atTGGCTATTCCGACTCAGC‑AAAAGATGCGGGAGTGATGGCAGCTTCCTGGGCGCAATATCAGGCACAg < 1:1799534/69‑1 (MQ=255) atTGGCTATTCCGACTCAGC‑AAAAGATGCGGGAGTGATGGCAGCTTCCTGGGCGCAATATCAGGCACAg < 1:44522/69‑1 (MQ=255) atTGGCTATTCCGACTCAGC‑AAAAGATGCGGGAGTGATGGCAGCTTCCTGGGCGCAATATCAGGCACAg > 1:541765/1‑69 (MQ=255) atTGGCTATTCCGACTCAGC‑AAAAGATGCGGGAGTGATGGCAGCTTCCTGGGCGCAATATCAGGCACAg < 1:2665561/69‑1 (MQ=255) atTGGCTATTCCGACTCAGC‑AAAAGATGCGGGAGTGATGGCAGCTTCCTGGGCGCAATATCAGGCACAg < 1:607732/69‑1 (MQ=255) atTGGCTATTCCGACTCAGC‑AAAAGATGCGGGAGTGATGGCAGCTTCCTGGGCGCAATATCAGGCACAg < 1:891391/69‑1 (MQ=255) ttGGCTATTCCGACTCAGC‑AAAAGATGCGGGAGTGATGGCAGCTTCCTGGGCGCAATATCAGGCACAg < 1:759187/68‑1 (MQ=255) tGGCTATTCCGACTCAGC‑AAAAGATGCGGGAGTGATGTCAGCTTCCTGGGCGCAATATCAGGCACAGGa > 1:1955730/1‑69 (MQ=255) tGGCTATTCCGACTCAGC‑AAAAGATGCGGGAGTGATGGCAGCTTCCTGGGCGCAATATCAGGca < 1:2641209/64‑1 (MQ=255) tGGCTATTCCGACTCAGC‑AAAAGATGCGGGAGTGATGGCAGCTTCCTGGGCGCAATATCAGGCACAgg > 1:399671/1‑68 (MQ=255) tGGCTATTCCGACTCAGC‑AAAAGATGCGGGAGTGATGGCAGCTTCCTGGGCGCAATATCAGGCACAGGa > 1:1089568/1‑69 (MQ=255) tGGCTATTCCGACTCAGC‑AAAAGATGCGGGAGTGATGGCAGCTTCCTGGGCGCAATATCAGGCACAGGa > 1:1468879/1‑69 (MQ=255) tGGCTATTCCGACTCAGC‑AAAAGATGCGGGAGTGATGGCAGCTTCCTGGGCGCAATATCAGGCACAGGa > 1:1802725/1‑69 (MQ=255) cTATTCCGACTCAGC‑AAAAGATGCGGGAGTGATGGCAGCTTCCTGGGCGCAATATCAGGCACAGGATg < 1:902941/68‑1 (MQ=255) cTATTCCGACTCAGC‑AAAAGATGCGGGAGTGATGGCAGCTTCCTGGGCGCAATATCAGGCACAGGATg < 1:508499/68‑1 (MQ=255) cTATTCCGACTCAGC‑AAAAGATGCGGGAGTGATGGCAGCTTCCTGGGCGCAATATCAGGCACAGGATg < 1:2054437/68‑1 (MQ=255) cTATTCCGACTCAGC‑AAAAGATGCGGGAGTGATGGCAGCTTCCTGGGCGCAATATCAGGCACAGGATg < 1:3433769/68‑1 (MQ=255) cTATTCCGACTCAGC‑AAAAGATGCGGGAGTGATGGCAGCTTCCTGGGCGCAATATCAGGCACAGGATg < 1:3282842/68‑1 (MQ=255) cTATTCCGACTCAGC‑AAAAGATGCGGGAGTGATGGCAGCTTCCTGGGCGCAATATCAGGCACAGGATg < 1:3217457/68‑1 (MQ=255) cTATTCCGACTCAGC‑AAAAGATGCGGGAGTGATGGCAGCTTCCTGGGCGCAATATCAGGCACAGGATg < 1:1696148/68‑1 (MQ=255) aatCCGACTCAGCAAAAAGATGCGGGAGTGATGGCAGCTTCCTGGGCGCAATATCAGGCACAGGATGCa < 1:456184/67‑1 (MQ=255) aTTCCGACTCAGCAAAAAGATGCGGGAGTGATGGCAGCTTCCTGGGCGCAATATCAGGCACAGGATGCa < 1:2278713/69‑1 (MQ=255) aTTCCGACTCAGCAAAAAGATGCGGGAGTGATGGCAGCTTCCTGGGCGCAATATCAGGCACAGGATGCa < 1:506038/69‑1 (MQ=255) aTTCCGACTCAGCAAAAAGATGCGGGAGTGATGGCAGCTTCCTGGGCGCAATATCAGGCACAGGATGCa < 1:378089/69‑1 (MQ=255) aTTCCGACTCAGCAAAAAGATGCGGGAGTGATGGCAGCTTCCTGGGCGCAATATCAGGCACAGGATGCa < 1:1723156/69‑1 (MQ=255) aTTCCGACTCAGCAAAAAGATGCGGGAGTGATGGCAGCTTCCTGGGCGCAATATCAGGCACAGGATGCa < 1:2038451/69‑1 (MQ=255) aTTCCGACTCAGCAAAAAGATGCGGGAGTGATGGCAGCTTCCTGGGCGCAATATCAGGCACAGGATGCa < 1:2709150/69‑1 (MQ=255) ccGACTCAGC‑AAAAGATGCGGGAGTGATGGCAGCTTCCTGGGCGCAATATCAGGCACAGGATGCATTa > 1:2897210/1‑68 (MQ=255) ccGACTCAGC‑AAAAGATGCGGGAGTGATGGCAGCTTCCTGGGCGCAATATCAGGCACAGGATGCATTa > 1:1717884/1‑68 (MQ=255) ccGACTCAGC‑AAAAGATGCGGGAGTGATGGCAGCTTCCTGGGCGCAATATCAGGCACAGGATGCATTa > 1:2164818/1‑68 (MQ=255) ccGACTCAGC‑AAAAGATGCGGGAGTGATGGCAGCTTCCTGGGCGCAATATCAGGCACAGGATGCATTa > 1:1202429/1‑68 (MQ=255) ccGACTCAGC‑AAAAGATGCGGGAGTGATGGCAGCTTCCTGGGCGCAATATCAGGCACAGGATGCATTa > 1:1291402/1‑68 (MQ=255) ccGACTCAGC‑AAAAGATGCGGGAGTGATGGCAGCTTCCTGGGCGCAATATCAGGCACAGGATGCATTa > 1:1366470/1‑68 (MQ=255) ccGACTCAGC‑AAAAGATGCGGGAGTGATGGCAGCTTCCTGGGCGCAATATCAGGCACAGGATGCATTa > 1:1473509/1‑68 (MQ=255) ccGACTCAGC‑AAAAGATGCGGGAGTGATGGCAGCTTCCTGGGCGCAATATCAGGCACAGGATGCATTa > 1:3167680/1‑68 (MQ=255) aCTCAGC‑AAAAGATGCGGGCTTGATGGCAGCTTCCTGGGCGCAATATCAGGCACAGGATGCATTAATc < 1:3140394/68‑1 (MQ=255) aCTCAGC‑AAAAGATGCGGGCGTGATGGCAGCTTCCTGGGCGCAATATCAGGCACAGGATGCATTAATc < 1:91838/68‑1 (MQ=255) aCTCAGC‑AAAAGATGCGGGCGTGATGGCAGCTTCCTGGGCGCAATATCAGGCACAGGATGCATTAATc < 1:2099077/68‑1 (MQ=255) aCTCAGC‑AAAAGATGCGGGCGTGATGGCAGCTTCCTGGGCGCAATATCAGGCACAGGATGCATTAATc < 1:2653157/68‑1 (MQ=255) aCTCAGC‑AAAAGATGCGGGCGTGATGGCAGCTTCCTGGGCGCAATATCAGGCACAGGATGCATTAATc < 1:1405220/68‑1 (MQ=255) aCTCAGC‑AAAAGATGCGGGCGTGATGGCAGCTTCCTGGGCGCAATATCAGGCACAGGATGCATTAATc < 1:1206136/68‑1 (MQ=255) aCTCAGC‑AAAAGATGCGGGCGTGATGGCAGCTTCCTGGGCGCAATATCAGGCACAGGATGCATTAATc < 1:1439194/68‑1 (MQ=255) aCTCAGC‑AAAAGATGCGGGCGTGATGGCAGCTTCCTGGGCGCAATATCAGGCACAGGATGCATTAATc < 1:3278132/68‑1 (MQ=255) aCTCAGC‑AAAAGATGCGGGCGTGATGGCAGCTTCCTGGGCGCAATATCAGGCACAGGATGCATTAATc < 1:3297945/68‑1 (MQ=255) aCTCAGC‑AAAAGATGCGGGCGTGATGGCAGCTTCCTGGGCGCAATATCAGGCACAGGATGCATTAATc < 1:3057532/68‑1 (MQ=255) aCTCAGC‑AAAAGATGCGGGCGTGATGGCAGCTTCCTGGGCGCAATATCAGGCACAGGATGCATTAATc < 1:1577548/68‑1 (MQ=255) aCTCAGC‑AAAAGATGCGGGCGTGATGGCAGCTTCCTGGGCGCAATATCAGGCACAGGATGCATTAATc < 1:2976431/68‑1 (MQ=255) aCTCAGC‑AAAAGATGCGGGAGTGATGGCAGCTTCCTGGGCGCAATATCAGGCACAGGATGCATTAATc < 1:2349855/68‑1 (MQ=255) cTCAGC‑AAAAGATGCGGGCGTGATGGCAGCTTCCTGGGCGCAATATCAGGCACAGGATGCATTAATc < 1:2570749/67‑1 (MQ=255) tCAGC‑AAAAGATGCGGGAGTGATGGCAGCTTCCTGGGCGCAATATCAGGCACAGGATGCATTAATCaaa > 1:3268747/1‑69 (MQ=255) tGCGGGCGTGATGGCAGCTTCCTGGGCGCAATATCAGGCACAGGATGCATTAATc < 1:584791/55‑1 (MQ=255) gggCGTGATGGCAGCTTCCTGGGCGCAATATCAGGCACAGGATGCATTAATc < 1:1381952/52‑1 (MQ=255) gggAGTGATGGCAGCTTCCTGGGCGCAATATCAGGca < 1:1125711/37‑1 (MQ=255) aGTGATGGCAGCTTCCTGGGCGCAATATCAGGCACAGGATGCATTAATCAAAACCTGCGAAAAAGCggg < 1:1797601/69‑1 (MQ=255) aGTGATGGCAGCTTCCTGGGCGCAATATCAGGCACAGGATGCATTAATCAAAACCTGCGAAAAAGCggg < 1:2964054/69‑1 (MQ=255) aGTGATGGCAGCTTCCTGGGCGCAATATCAGGCACAGGATGCATTAATCAAAACCTGCGAAAAAGCggg < 1:901193/69‑1 (MQ=255) aGTGATGGCAGCTTCCTGGGCGCAATATCAGGCACAGGATGCATTAATCAAAACCTGCGAAAAAGCggg < 1:1117849/69‑1 (MQ=255) aGTGATGGCAGCTTCCTGGGCGCAATATCAGGCACAGGATGCATTAATCAAAACCTGCGAAAAAGCggg < 1:1217788/69‑1 (MQ=255) aGTGATGGCAGCTTCCTGGGCGCAATATCAGGCACAGGATGCATTAATCAAAACCTGCGAAAAAGCggg < 1:2983189/69‑1 (MQ=255) aGTGATGGCAGCTTCCTGGGCGCAATATCAGGCACAGGATGCATTAATCAAAACCTGCGAAAAAGCggg < 1:514487/69‑1 (MQ=255) aGTGATGGCAGCTTCCTGGGCGCAATATCAGGCACAGGATGCATTAATCAAAACCTGCGAAAAAGCggg < 1:1315135/69‑1 (MQ=255) aGTGATGGCAGCTTCCTGGGCGCAATATCAGGCACAGGATGCATTAATCAAAACCTGCGAAAAAGCggg < 1:2065532/69‑1 (MQ=255) aGTGATGGCAGCTTCCTGGGCGCAATATCAGGCACAGGATGCATTAATCAAAACCTGCGAAAAAGCggg < 1:2021812/69‑1 (MQ=255) | TCGTGGCCTGATTCAGGGCAAACAGATGGTGATGATTGGCTATTCCGACTCAGC‑AAAAGATGCGGGAGTGATGGCAGCTTCCTGGGCGCAATATCAGGCACAGGATGCATTAATCAAAACCTGCGAAAAAGCGGG > minE/2290869‑2291003 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |