| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,291,520 | C→T | 31.0% | D744D (GAC→GAT) | ppc → | phosphoenolpyruvate carboxylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,291,520 | 0 | C | T | 31.0% | 69.0 / 34.0 | 74 | D744D (GAC→GAT) | ppc | phosphoenolpyruvate carboxylase |
| Reads supporting (aligned to +/- strand): ref base C (23/28); new base T (12/11); total (35/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.21e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01 | |||||||||||
TGATGCTCCCCGCCTGGCTGGGTGCAGGTACGGCGCTGCAAAAAGTGGTCGAAGACGGCAAACAGAGCGAGCTGGAGGCTATGTGCCGCGATTGGCCATTCTTC > minE/2291465‑2291568 | tGATGCTCCCCGCCTGGCTGGGTGCAGGTACGGCGCTGCAAAAAGTGGTCGAAGATGGCAAACAgagcg > 1:3408791/1‑69 (MQ=255)tGATGCTCCCCGCCTGGCTGGGTGCAGGTACGGCGCTGCAAAAAGTGGTCGAAGATGGCAAACAgagcg > 1:2628736/1‑69 (MQ=255)tGATGCTCCCCGCCTGGCTGGGTGCAGGTACGGCGCTGCAAAAAGTGGTCGAAGATGGCAAACAgagcg > 1:2603959/1‑69 (MQ=255)tGATGCTCCCCGCCTGGCTGGGTGCAGGTACGGCGCTGCAAAAAGTGGTCGAAGATGGCAAACAgagcg > 1:25276/1‑69 (MQ=255)tGATGCTCCCCGCCTGGCTGGGTGCAGGTACGGCGCTGCAAAAAGTGGTCGAAGATGGCAAACAgagcg > 1:1057080/1‑69 (MQ=255)tGATGCTCCCCGCCTGGCTGGGTGCAGGTACGGCGCTGCAAAAAGTGGTCGAAGATGGCAAACAgagcg > 1:2862858/1‑69 (MQ=255)tGATGCTCCCCGCCTGGCTGGGTGCAGGTACGGCGCTGCAAAAAGTGGTCGAAGATGGCAAACAgagcg > 1:1715618/1‑69 (MQ=255)tGATGCTCCCCGCCTGGCTGGGTGCAGGTACGGCGCTGCAAAAAGTGGTCGAAGATGGCAAACAgagcg > 1:49278/1‑69 (MQ=255)tGATGCTCCCCGCCTGGCTGGGTGCAGGTACGGCGCTGCAAAAAGTGGTCGAAGATGGCAAACAgagcg > 1:1098745/1‑69 (MQ=255)tGATGCTCCCCGCCTGGCTGGGTGCAGGTACGGCGCTGCAAAAAGTGGTCGAAGATGGCAAACAgagcg > 1:784504/1‑69 (MQ=255)tGATGCTCCCCGCCTGGCTGGGTGCAGGTACGGCGCTGCAAAAAGTGGTCGAAGATGGCAAACAgagcg > 1:1348284/1‑69 (MQ=255)tGATGCTCCCCGCCTGGCTGGGTGCAGGTACGGCGCTGCAAAAAGTGGTCGAAGATGGCAAACAgagc > 1:806642/1‑68 (MQ=255) ccTGGCTGGGTGCAGGTACGGCGCTGCAAAAAGTGGTCGAAGACGGCAAACAGAGCGAGCTGGAGGCTa < 1:2596737/69‑1 (MQ=255) tGCAGGTACGGCGCTGCAAAAAGTGGTCGAAGACGGCAAACAGAGCGAGCTGGAGGCTATGTGCcgcg < 1:1074251/68‑1 (MQ=255) tGCAGGTACGGCGCTGCAAAAAGTGGTCGAAGACGGCAAACAGAGCGAGCTGGAGGCTATGTGCcgcg < 1:770551/68‑1 (MQ=255) tGCAGGTACGGCGCTGCAAAAAGTGGTCGAAGACGGCAAACAGAGCGAGCTGGAGGCTATGTGCcgcg < 1:231008/68‑1 (MQ=255) tGCAGGTACGGCGCTGCAAAAAGTGGTCGAAGACGGCAAACAGAGCGAGCTGGAGGCTATGTGCcgcg < 1:206225/68‑1 (MQ=255) tGCAGGTACGGCGCTGCAAAAAGTGGTCGAAGACGGCAAACAGAGCGAGCTGGAGGCTATGTGCcgcg < 1:2858849/68‑1 (MQ=255) tGCAGGTACGGCGCTGCAAAAAGTGGTCGAAGACGGCAAACAGAGCGAGCTGGAGGCTATGTGCcgcg < 1:2892597/68‑1 (MQ=255) tGCAGGTACGGCGCTGCAAAAAGTGGTCGAAGACGGCAAACAGAGCGAGCTGGAGGCTATGTGCcgcg < 1:1867232/68‑1 (MQ=255) tGCAGGTACGGCGCTGCAAAAAGTGGTCGAAGACGGCAAACAGAGCGAGCTGGAGGCTATGTGCcgcg < 1:569604/68‑1 (MQ=255) tGCAGGTACGGCGCTGCAAAAAGTGGTCGAAGACGGCAAACAGAGCGAGCTGGAGGCTATGTGCcgcg < 1:1523895/68‑1 (MQ=255) gTACGGCGCTGCAAAAAGTGGTCGAAGACGGCAAACAGAGCGAGCTGGAGGCTATGTGCCGCGATTGGc < 1:1811363/69‑1 (MQ=255) gTACGGCGCTGCAAAAAGTGGTCGAAGACGGCAAACAGAGCGAGCTGGAGGCTATGTGCCGCGATTGGc < 1:2075945/69‑1 (MQ=255) gTACGGCGCTGCAAAAAGTGGTCGAAGACGGCAAACAGAGCGAGCTGGAGGCTATGTGCCGCGATTGGc < 1:1163153/69‑1 (MQ=255) gTACGGCGCTGCAAAAAGTGGTCGAAGACGGCAAACAGAGCGAGCTGGAGGCTATGTGCCGCGATTGGc < 1:2476481/69‑1 (MQ=255) gTACGGCGCTGCAAAAAGTGGTCGAAGACGGCAAACAGAGCGAGCTGGAGGCTATGTGCCGCGATTGGc < 1:265337/69‑1 (MQ=255) accgcgcTGCAAAAAGTGGTCGAAGACGGCAAACAGAGCGAGCTGGAGGCTATGTGCCGCGATTGGCCa > 1:2708880/4‑69 (MQ=255) aCGGCGCTTCAAAAAGTGGTCGAAGACGGCAAACAGAGCGAGCTGGAGGCTa < 1:434854/52‑1 (MQ=255) aCGGCGCTGCAAAAAGTGGTCGAAGACGGCTAACAGAGCGAGCTGGAGGCTATGTGCCGCGAt > 1:735283/1‑63 (MQ=255) aCGGCGCTGCAAAAAGTGGTCGAAGACGGCAATCAGAGCGAGCTGGAGGCTATGTGCCGCGATTGGcc > 1:2908785/1‑68 (MQ=255) aCGGCGCTGCAAAAAGTGGTCGAAGACGGCAAACAGAGCGAGCTg > 1:2088038/1‑45 (MQ=255) aCGGCGCTGCAAAAAGTGGTCGAAGACGGCAAACAGAGCGAGCTGGAGGCTa < 1:2672571/52‑1 (MQ=255) aCGGCGCTGCAAAAAGTGGTCGAAGACGGCAAACAGAGCGAGCTGGAGGCTa > 1:415632/1‑52 (MQ=255) aCGGCGCTGCAAAAAGTGGTCGAAGACGGCAAACAGAGCGAGCTGGAGGCTa < 1:844097/52‑1 (MQ=255) aCGGCGCTGCAAAAAGTGGTCGAAGACGGCAAACAGAGCGAGCTGGAGGCTa < 1:1241865/52‑1 (MQ=255) aCGGCGCTGCAAAAAGTGGTCGAAGACGGCAAACAGAGCGAGCTGGAGGCTa < 1:2057705/52‑1 (MQ=255) aCGGCGCTGCAAAAAGTGGTCGAAGACGGCAAACAGAGCGAGCTGGAGGCTa > 1:1622799/1‑52 (MQ=255) aCGGCGCTGCAAAAAGTGGTCGAAGACGGCAAACAGAGCGAGCTGGAGGCTa < 1:180450/52‑1 (MQ=255) aCGGCGCTGCAAAAAGTGGTCGAAGACGGCAAACAGAGCGAGCTGGAGGCTa < 1:1833797/52‑1 (MQ=255) aCGGCGCTGCAAAAAGTGGTCGAAGACGGCAAACAGAGCGAGCTGGAGGCTa < 1:1863598/52‑1 (MQ=255) aCGGCGCTGCAAAAAGTGGTCGAAGACGGCAAACAGAGCGAGCTGGAGGCTATGTGCCGCGATTGGcc > 1:943448/1‑68 (MQ=255) aCGGCGCTGCAAAAAGTGGTCGAAGACGGCAAACAGAGCGAGCTGGAGGCTATGTGCCGCGATTGGcc > 1:586173/1‑68 (MQ=255) aCGGCGCTGCAAAAAGTGGTCGAAGACGGCAAACAGAGCGAGCTGGAGGCTATGTGCCGCGATTGGcc > 1:54044/1‑68 (MQ=255) aCGGCGCTGCAAAAAGTGGTCGAAGACGGCAAACAGAGCGAGCTGGAGGCTATGTGCCGCGATTGGcc > 1:1799668/1‑68 (MQ=255) aCGGCGCTGCAAAAAGTGGTCGAAGACGGCAAACAGAGCGAGCTGGAGGCTATGTGCCGCGATTGGc < 1:261250/67‑1 (MQ=255) aCGGCGCTGCAAAAAGTGGTCGAAGACGGCAAACAGAGCGAGCTGGAGGCTATGTGCCGCGATTGGc < 1:3063415/67‑1 (MQ=255) aCGGCGCTGCAAAAAGTGGTCGAAGACGGCAAACAGAGCGAGCTGGAGGCTATGTGCCGCGATTGGCCa > 1:2803485/1‑69 (MQ=255) aCGGCGCTGCAAAAAGTGGTCGAAGACGGCAAACAGAGCGAGCTGGAGGCTATGTGCCGCGATTGGCCa > 1:1126382/1‑69 (MQ=255) aCGGCGCTGCAAAAAGTGGTCGAAGACGGCAAACAGAGCGAGCTGGAGGCTATGTGCCGCGATTGGCCa > 1:1383582/1‑69 (MQ=255) aCGGCGCTGCAAAAAGTGGTCGAAGACGGCAAACAGAGCGAGCTGGAGGCTATGTGCCGCGATTGGCCa > 1:2416953/1‑69 (MQ=255) aCGGCGCTGCAAAAAGTGGTCGAAGACGGCAAACAGAGCGAGCTGGAGGCTATGTGCCGCGATTGGCCa > 1:2745789/1‑69 (MQ=255) aCGGCGCTGCAAAAAGTGGTCGAAGACGGCAAACAGAGCGAGCTGGAGGCTATGTGCCGCGATTGGCCa > 1:548278/1‑69 (MQ=255) aCGGCGCTGCAAAAAGTGGTCGAAGACGGCAAACAGAGCGAGCTGGAGGCTATGTGCCGCGATTGGCCa > 1:2014625/1‑69 (MQ=255) aCGGCGCTGCAAAAAGTGGTCGAAGACGGCAAACAGAGCGAGCTGGAGGCTATGTGCCGCGATTGGCCa > 1:297581/1‑69 (MQ=255) aCGGCGCTGCAAAAAGTGGTCGAAGACGGCAAACAGAGCGAGCTGGAGGCTATGTGCCGCGATTGGCCa > 1:3053410/1‑69 (MQ=255) aCGGCGCTGCAAAAAGTGGTCGAAGACGGCAAACAGAGCGAGCTGGAGGCTATGTGCCGCGATTGGCCa > 1:2025793/1‑69 (MQ=255) aCGGCGCTGCAAAAAGTGGTCGAAGACGGCAAACAGAGCGAGCTGGAGGCTATGTGCCGCGATTGGCCa > 1:327579/1‑69 (MQ=255) aCGGCGCTGCAAAAAGTGGTCGAAGACGGCAAACAGAGCGAGCTGGAGGCTATGTGCCGCGATTGGCCa > 1:2835052/1‑69 (MQ=255) cGGCGCTGCAAAAAGTGGTCGAAGACGGCAAACAGAGCGAGCTGGAGGCTATGTGCcgcg < 1:843124/60‑1 (MQ=255) gcgcTGCAAAAAGTGGTCGAAGACGGCAAACAGAGCGAGCTGGAGGCTATGTGCCGCGATTGGc < 1:369294/64‑1 (MQ=255) aaGTGGTCGAAGATGGCAAACAGAGCGAGCTGGAAGCCATGTGCcgc < 1:3095189/47‑1 (MQ=38) aaGTGGTCGAAGATGGCAAACAGAGCGAGCTGGAAGCCATGTGCcgc < 1:2018474/47‑1 (MQ=38) aaGTGGTCGAAGATGGCAAACAGAGCGAGCTGGAAGCCATGTGCcgc < 1:2562183/47‑1 (MQ=38) aaGTGGTCGAAGATGGCAAACAGAGCGAGCTGGAAGCCATGTGCcgc < 1:1501202/47‑1 (MQ=38) aaGTGGTCGAAGATGGCAAACAGAGCGAGCTGGAAGCCATGTGCcgc < 1:1539627/47‑1 (MQ=38) aaGTGGTCGAAGATGGCAAACAGAGCGAGCTGGAAGCCATGTGCcgc < 1:674986/47‑1 (MQ=38) aaGTGGTCGAAGATGGCAAACAGAGCGAGCTGGAAGCCATGTGCcgc < 1:556001/47‑1 (MQ=38) aaGTGGTCGAAGATGGCAAACAGAGCGAGCTGGAAGCCATGTGCcgc < 1:540665/47‑1 (MQ=38) aaGTGGTCGAAGATGGCAAACAGAGCGAGCTGGAAGCCATGTGCcgc < 1:528573/47‑1 (MQ=38) aaGTGGTCGAAGATGGCAAACAGAGCGAGCTGGAAGCCATGTGCcgc < 1:1629367/47‑1 (MQ=38) aaGTGGTCGAAGATGGCAAACAGAGCGAGCTGGAAGCCATGTGCcgc < 1:2810648/47‑1 (MQ=38) aaGTGGTCGAAGACGGCAAACAGAGCGAGCTGGAGGCTATGTGCcgcg < 1:818118/48‑1 (MQ=255) tCGAAGACGGCAAACAGAGCGAGCTGGAGGCTATGTGCCGCGATTGGCCAttcttc > 1:501306/1‑56 (MQ=255) | TGATGCTCCCCGCCTGGCTGGGTGCAGGTACGGCGCTGCAAAAAGTGGTCGAAGACGGCAAACAGAGCGAGCTGGAGGCTATGTGCCGCGATTGGCCATTCTTC > minE/2291465‑2291568 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |