| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,292,920 | G→A | 36.4% | V17V (GTG→GTA) | gldA → | glycerol dehydrogenase, NAD |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,292,920 | 0 | G | A | 36.4% | 47.0 / 43.4 | 74 | V17V (GTG→GTA) | gldA | glycerol dehydrogenase, NAD |
| Reads supporting (aligned to +/- strand): ref base G (9/38); new base A (18/9); total (27/47) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.08e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.01e-01 | |||||||||||
TCTAAAGGAGCAATTATGGACCGCATTATTCAATCACCGGGTAAATACATCCAGGGCGCTGATGTGATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAGTGGTGGGTGACAAATTT > minE/2292855‑2292986 | tctAAAGGAGCAATTATGGACCGCATTATTCAATCACCGGGTAAATACATCCAGGGCGCTGATGTGAtt > 1:2350894/1‑69 (MQ=255)tctAAAGGAGCAATTATGGACCGCATTATTCAATCACCGGGTAAATACATCCAGGGCGCTGATGTGAtt > 1:1440358/1‑69 (MQ=255) aaGGAGCAATTATGGACCGCATTATTCAATCACCGGGTAAATACATCCAGGGCGCTGATGTGATTa < 1:3349752/66‑1 (MQ=255) aaGGAGCAATTATGGACCGCATTATTCAATCACCGGGTAAATACATCCAGGGCGCTGATGTGATTa < 1:3314933/66‑1 (MQ=255) aGGAGCAATTATGGACCGCATTATTCAATCACCGGGTAAATACATCCAGGGCGCTGATGTGATTa < 1:678690/65‑1 (MQ=255) ggACCGCATTATTCAATCACCAGGTAAATACATCCAGGGCGCAGATGTAATTAATCGTCTGGGCGAGTa > 1:1458697/1‑69 (MQ=255) ggACCGCATTATTCAATCACCAGGTAAATACATCCAGGGCGCAGATGTAATTAATCGTCTGGGCGAGTa > 1:2273404/1‑69 (MQ=255) ggACCGCATTATTCAATCACCAGGTAAATACATCCAGGGCGCAGATGTAATTAATCGTCTGGGCGAGTa > 1:2432503/1‑69 (MQ=255) ggACCGCATTATTCAATCACCAGGTAAATACATCCAGGGCGCAGATGTAATTAATCGTCTGGGCGAGTa > 1:2456981/1‑69 (MQ=255) ggACCGCATTATTCAATCACCAGGTAAATACATCCAGGGCGCAGATGTAATTAATCGTCTGGGCGAGTa > 1:2615876/1‑69 (MQ=255) ggACCGCATTATTCAATCACCAGGTAAATACATCCAGGGCGCAGATGTAATTAATCGTCTGGGCGAGTa > 1:2618197/1‑69 (MQ=255) ggACCGCATTATTCAATCACCAGGTAAATACATCCAGGGCGCAGATGTAATTAATCGTCTGGGCGAGTa > 1:2741177/1‑69 (MQ=255) ggACCGCATTATTCAATCACCAGGTAAATACATCCAGGGCGCAGATGTAATTAATCGTCTGGGCGAGTa > 1:2876887/1‑69 (MQ=255) gACCGCATTATTCAATCACCGGGTAAATACATCCAGGGCGCTGATGTGATTAATCGTCTGGGCGAATAc < 1:2685365/69‑1 (MQ=255) gACCGCATTATTCAATCACCGGGTAAATACATCCAGGGCGCTGATGTGATTAATCGTCTGGGCGAATAc < 1:383451/69‑1 (MQ=255) gACCGCATTATTCAATCACCGGGTAAATACATCCAGGGCGCTGATGTGATTAATCGTCTGGGCGAATAc < 1:563595/69‑1 (MQ=255) gACCGCATTATTCAATCACCGGGTAAATACATCCAGGGCGCTGATGTGATTAATCGTCTGGGCGAATAc < 1:1050250/69‑1 (MQ=255) tattCAATCACCGGGTAAATACATCCAGGGCGCTGATGTGATTAATCGTCTGGGCGAATACCTGAAGcc < 1:2683447/69‑1 (MQ=255) tattCAATCACCGGGTAAATACATCCAGGGCGCTGATGTGATTAATCGTCTGGGCGAATACCTGAAGcc < 1:1718847/69‑1 (MQ=255) attCAATCACCGGGTAAATACATCCAGGGCGCTGATGTGATTAATCg < 1:3128102/47‑1 (MQ=255) cAATCACCGGGTAAATACATCCAGGGCGCTGATGTGATTAATCGTCTGGGCGAATACCTGAAGCCGCt > 1:2676060/1‑68 (MQ=255) aTCACCGGGTAAATACATCCAGGGCGCTGATTTGATTa < 1:2939714/38‑1 (MQ=255) cACCGGGTAAATACATCCAGGGCGCTGATGTGATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAg > 1:3322517/1‑69 (MQ=255) cACCGGGTAAATACATCCAGGGCGCTGATGTGATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAg > 1:874063/1‑69 (MQ=255) cACCGGGTAAATACATCCAGGGCGCTGATGTGATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAg > 1:2087325/1‑69 (MQ=255) cACCGGGTAAATACATCCAGGGCGCTGATGTGATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAg > 1:2293560/1‑69 (MQ=255) gTAAATACATCCAGGGCGCTGATGTGATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCt > 1:2841408/1‑69 (MQ=255) tAAATACATCCAGGGCGCTGATGTGATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTg > 1:1767340/1‑69 (MQ=255) tACATCCAGGGCGCTGATGTGATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGtt < 1:3024846/68‑1 (MQ=255) tACATCCAGGGCGCTGATGTGATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGtt < 1:1973186/68‑1 (MQ=255) tACATCCAGGGCGCTGATGTGATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGtt < 1:1503620/68‑1 (MQ=255) cATCCAGGGCGCTGATGTGATTAATCGTCTTGGCGAATACCTGAAGCCTCTGGCAGAACGCTGGTTAgt < 1:1583326/69‑1 (MQ=255) cATCCAGGGCGCTGATGTGATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAgt < 1:757274/69‑1 (MQ=255) cATCCAGGGCGCTGATGTGATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAgt < 1:1657349/69‑1 (MQ=255) cATCCAGGGCGCTGATGTGATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAgt < 1:828978/69‑1 (MQ=255) cATCCAGGGCGCTGATGTGATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAgt < 1:2880791/69‑1 (MQ=255) cATCCAGGGCGCTGATGTGATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAgt < 1:3431756/69‑1 (MQ=255) cATCCAGGGCGCTGATGTGATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAgt < 1:1745337/69‑1 (MQ=255) cATCCAGGGCGCTGATGTGATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAgt < 1:3087292/69‑1 (MQ=255) ccAGGGCGCTGATGTGATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAgtggt < 1:577405/69‑1 (MQ=255) ccAGGGCGCTGATGTGATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAgtggt < 1:2858857/69‑1 (MQ=255) ccAGGGCGCTGATGTGATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAgtggt < 1:3007164/69‑1 (MQ=255) ccAGGGCGCTGATGTGATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAgtggt < 1:3285427/69‑1 (MQ=255) ccAGGGCGCTGATGTGATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAgtggt < 1:1013713/69‑1 (MQ=255) ccAGGGCGCTGATGTGATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAgtggt < 1:941278/69‑1 (MQ=255) ccAGGGCGCTGATGTGATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAgtggt < 1:159357/69‑1 (MQ=255) ccAGGGCGCTGATGTGATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAgtggt < 1:1905826/69‑1 (MQ=255) ccAGGGCGCTGATGTGATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAgtggt < 1:2088787/69‑1 (MQ=255) ccAGGGCGCTGATGTGATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAgtggt < 1:824104/69‑1 (MQ=255) cAGGGCGCTGATGTGATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAg < 1:767971/52‑1 (MQ=255) cAGGGCGCTGATGTGATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAg < 1:1867118/52‑1 (MQ=255) cAGGGCGCTGATGTGATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAg < 1:1145431/52‑1 (MQ=255) cAGGCCGCTGATGTGATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAg < 1:2647175/52‑1 (MQ=255) aGGGCGCTGATGTGATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAg < 1:236127/51‑1 (MQ=255) aGGGCGCAGATGTAATTAATCGTCTGGGCGAGTACCTGAAGCCACTGGCGGAACGCTGGTTAGtggtgg > 1:588443/1‑69 (MQ=255) aGGGCGCAGATGTAATTAATCGTCTGGGCGAGTACCTGAAGCCACTGGCGGAACGCTGGTTAGtggtgg > 1:1070066/1‑69 (MQ=255) aGGGCGCAGATGTAATTAATCGTCTGGGCGAGTACCTGAAGCCACTGGCGGAACGCTGGTTAGtggtgg > 1:2425280/1‑69 (MQ=255) aGGGCGCAGATGTAATTAATCGTCTGGGCGAGTACCTGAAGCCACTGGCGGAACGCTGGTTAGtggtgg > 1:2608012/1‑69 (MQ=255) aGGGCGCAGATGTAATTAATCGTCTGGGCGAGTACCTGAAGCCACTGGCGGAACGCTGGTTAGtggtgg > 1:3413644/1‑69 (MQ=255) aGGGCGCAGATGTAATTAATCGTCTGGGCGAGTACCTGAAGCCACTGGCGGAACGCTGGTTAGtggtgg > 1:2047639/1‑69 (MQ=255) aGGGCGCAGATGTAATTAATCGTCTGGGCGAGTACCTGAAGCCACTGGCGGAACGCTGGTTAGtggtgg > 1:1924559/1‑69 (MQ=255) aGGGCGCAGATGTAATTAATCGTCTGGGCGAGTACCTGAAGCCACTGGCGGAACGCTGGTTAGtggtgg > 1:140133/1‑69 (MQ=255) aGGGCGCAGATGTAATTAATCGTCTGGGCGAGTACCTGAAGCCACTGGCGGAACGCTGGTTAGtggtgg > 1:3324892/1‑69 (MQ=255) aGGGCGCAGATGTAATTAATCGTCTGGGCGAGTACCTGAAGCCACTGGCGGAACGCTGGTTAGtggtgg > 1:3272804/1‑69 (MQ=255) aTGTGATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAgtggt < 1:512828/58‑1 (MQ=255) aTGTAATTAATCGTCTGGTCGAGTACCTGAAGCCACTGGCGGAACGCTGGTTAGTGGTGGGTGACAAAt < 1:3417625/69‑1 (MQ=255) aTGTAATTAATCGTCTGGGCGAGTACCTGAAGCCACTGGCGGAACGCTGGTTAGTGGTGGGTGACAAAt < 1:2903540/69‑1 (MQ=255) aTGTAATTAATCGTCTGGGCGAGTACCTGAAGCCACTGGCGGAACGCTGGTTAGTGGTGGGTGACAAAt < 1:807632/69‑1 (MQ=255) aTGTAATTAATCGTCTGGGCGAGTACCTGAAGCCACTGGCGGAACGCTGGTTAGTGGTGGGTGACAAAt < 1:774212/69‑1 (MQ=255) aTGTAATTAATCGTCTGGGCGAGTACCTGAAGCCACTGGCGGAACGCTGGTTAGTGGTGGGTGACAAAt < 1:1869237/69‑1 (MQ=255) aTGTAATTAATCGTCTGGGCGAGTACCTGAAGCCACTGGCGGAACGCTGGTTAGTGGTGGGTGACAAAt < 1:2479502/69‑1 (MQ=255) aTGTAATTAATCGTCTGGGCGAGTACCTGAAGCCACTGGCGGAACGCTGGTTAGTGGTGGGTGACAAAt < 1:2545072/69‑1 (MQ=255) aTGTAATTAATCGTCTGGGCGAGTACCTGAAGCCACTGGCGGAACGCTGGTTAGTGGTGGGTGACAAAt < 1:1568136/69‑1 (MQ=255) aTGTAATTAATCGTCTGGGCGAGTACCTGAAGCCACTGGCGGAACGCTGGTTAGTGGTGGGTGACAAAt < 1:2635744/69‑1 (MQ=255) gtgATTAATCGTCTGGGCGAATACGTGAAGCCGCTGGCAGAACGCTGGTTAGTGGTGGGTGACAAAttt > 1:3295486/1‑69 (MQ=255) gtgATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCa > 1:1687898/1‑39 (MQ=255) gtgATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAGACCGc > 1:3000784/1‑45 (MQ=255) gtgATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAgt < 1:1832507/53‑1 (MQ=255) gtgATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAgt < 1:1826025/53‑1 (MQ=255) gtgATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAgt < 1:442187/53‑1 (MQ=255) gtgATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAgt < 1:1063984/53‑1 (MQ=255) gtgATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAGTGGTGGGTGACGAAttt > 1:2946399/1‑69 (MQ=255) gtgATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAGTGGTGGGTGACAAAttt > 1:2860437/1‑69 (MQ=255) gtgATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAGTGGTGGGTGACAAAttt > 1:1668464/1‑69 (MQ=255) gtgATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAGTGGTGGGTGACAAAttt > 1:2591944/1‑69 (MQ=255) gtgATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAGTGGTGGGTGACAAAttt > 1:755993/1‑69 (MQ=255) gtgATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAGTGGTGGGTGACAAAttt > 1:1292833/1‑69 (MQ=255) gtgATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAGTGGTGGGTGACAAAttt > 1:1428172/1‑69 (MQ=255) gtgATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAGTGGTGGGTGACAAAttt > 1:2894449/1‑69 (MQ=255) gtgATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAGTGGTGGGTGACAAAttt > 1:3272618/1‑69 (MQ=255) gtgATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAGTGGTGGGTGACAAAttt > 1:3304379/1‑69 (MQ=255) gtgATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAGTGGTGGGTGACAAAtt > 1:1079781/1‑68 (MQ=255) | TCTAAAGGAGCAATTATGGACCGCATTATTCAATCACCGGGTAAATACATCCAGGGCGCTGATGTGATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAGTGGTGGGTGACAAATTT > minE/2292855‑2292986 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |