| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,293,682 | C→T | 37.4% | H271H (CAC→CAT) | gldA → | glycerol dehydrogenase, NAD |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,293,682 | 0 | C | T | 37.4% | 58.0 / 59.7 | 96 | H271H (CAC→CAT) | gldA | glycerol dehydrogenase, NAD |
| Reads supporting (aligned to +/- strand): ref base C (39/21); new base T (9/27); total (48/48) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.79e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.34e-01 | |||||||||||
TGGCTGCGGCGCACGCAGTGCATAACGGCCTGACCGCTATCCCGGACGCGCATCACTATTATCACGGTGAAAAAGTGGCATTCGGTACGCTGACGCAGCTGGTTCTGGAAAATGCGCCGGTGG > minE/2293618‑2293740 | tGGCTGCGGCGCACGCAGTGCATAACGGCCTGACCGCTATCCCGGACGCGCATCACTATTATCACgg > 1:91612/1‑67 (MQ=255)tGGCTGCGGCGCACGCAGTGCATAACGGCCTGACCGCTATCCCGGACGCGCATCACTATTATCACGGt > 1:2805084/1‑68 (MQ=255) cacgcaGTGCATAACGGCCTGACCGCTATCCCGGACGCGCATCACTATTATCACGGTGAAAAAGTGGCa < 1:1556233/69‑1 (MQ=255) cacgcaGTGCATAACGGCCTGACCGCTATCCCGGACGCGCATCACTATTATCACGGTGAAAAAGTGGCa < 1:2053389/69‑1 (MQ=255) cacgcaGTGCATAACGGCCTGACCGCTATCCCGGACGCGCATCACTATTATCACGGTGAAAAAGTGGCa < 1:2996549/69‑1 (MQ=255) cacgcaGTGCATAACGGCCTGACCGCTATCCCGGACGCGCATCACTATTATCACGGTGAAAAAGTGGCa < 1:1561111/69‑1 (MQ=255) cacgcaGTGCATAACGGCCTGACCGCTATCCCGGACGCGCATCACTATTATCACGGTGAAAAAGTGGCa < 1:1318076/69‑1 (MQ=255) cacgcaGTGCATAACGGCCTGACCGCTATCCCGGACGCGCATCACTATTATCACGGTGAAAAAGTGGCa < 1:1122982/69‑1 (MQ=255) acgcaGTGCATAACGGCCTGACCGCTATCCCGGACGCGCATCACTATTATCACGGTGAAAAAGTGGCa < 1:2209509/68‑1 (MQ=255) aGTGCATAACGGCCTGACCGCTATCCCGGACGCGCATCACTATTATCACGGTGAAAAAGTGGCATTCgg < 1:1381352/69‑1 (MQ=255) aGTGCATAACGGCCTGACCGCTATCCCGGACGCGCATCACTATTATCACGGTGAAAAAGTGGCATTCgg < 1:610654/69‑1 (MQ=255) aGTGCATAACGGCCTGACCGCTATCCCGGACGCGCATCACTATTATCACGGTGAAAAAGTGGCATTCgg < 1:395694/69‑1 (MQ=255) aGTGCATAACGGCCTGACCGCTATCCCGGACGCGCATCACTATTATCACGGTGAAAAAGTGGCATTCgg < 1:235900/69‑1 (MQ=255) aGTGCATAACGGCCTGACCGCTATCCCGGACGCGCATCACTATTATCACGGTGAAAAAGTGGCATTCgg < 1:329510/69‑1 (MQ=255) cATAACGGCCTGACCGCTATCCCGGACGCGCATCACTATTATCACGGTGAAAAAGTGGCATTCGGTACg > 1:1090398/1‑69 (MQ=255) cATAACGGCCTGACCGCTATCCCGGACGCGCATCACTATTATCACGGTGAAAAAGTGGCATTCGGTACg > 1:2783554/1‑69 (MQ=255) cATAACGGCCTGACCGCTATCCCGGACGCGCATCACTATTATCACGGTGAAAAAGTGGCATTCGGTACg > 1:1826994/1‑69 (MQ=255) cATAACGGCCTGACCGCTATCCCGGACGCGCATCACTATTATCACGGTGAAAAAGTGGCATTCGGTACg > 1:585030/1‑69 (MQ=255) tAACGGCCTGACCGCTATCCCGGACGCGCATCACTATTATCATGGTGAAAAAGTGGCATTCGGTACGCt > 1:2869352/1‑69 (MQ=255) tAACGGCCTGACCGCTATCCCGGACGCGCATCACTATTATCATGGTGAAAAAGTGGCATTCGGTACGCt > 1:704064/1‑69 (MQ=255) tAACGGCCTGACCGCTATCCCGGACGCGCATCACTATTATCATGGTGAAAAAGTGGCATTCGGTACGCt > 1:1296638/1‑69 (MQ=255) tAACGGCCTGACCGCTATCCCGGACGCGCATCACTATTATCATGGTGAAAAAGTGGCATTCGGTACGCt > 1:1490282/1‑69 (MQ=255) tAACGGCCTGACCGCTATCCCGGACGCGCATCACTATTATCATGGTGAAAAAGTGGCATTCGGTACGCt > 1:1875720/1‑69 (MQ=255) tAACGGCCTGACCGCTATCCCGGACGCGCATCACTATTATCATGGTGAAAAAGTGGCATTCGGTACGCt > 1:199064/1‑69 (MQ=255) tAACGGCCTGACCGCTATCCCGGACGCGCATCACTATTATCATGGTGAAAAAGTGGCATTCGGTACGCt > 1:2238986/1‑69 (MQ=255) tAACGGCCTGACCGCTATCCCGGACGCGCATCACTATTATCATGGTGAAAAAGTGGCATTCGGTACGCt > 1:622530/1‑69 (MQ=255) tAACGGCCTGACCGCTATCCCGGACGCGCATCACTATTATCATGGTGAAAAAGTGGCATACGGTACGCt > 1:973526/1‑69 (MQ=255) tAACGGCCTGACCGCTATCCCGGACGCGCATCACTATTATCACGGTGAAAAAGTGGCATTCGGTACGCt < 1:3141775/69‑1 (MQ=255) tAACGGCCTGACCGCTATCCCGGACGCGCATCACTATTATCACGGTGAAAAAGTGGCATTCGGTACGCt < 1:2071039/69‑1 (MQ=255) tAACGGCCTGACCGCTATCCCGGACGCGCATCACTATTATCACGGTGAAAAAGTGGCATTCGGTACGCt < 1:2774866/69‑1 (MQ=255) tAACGGCCTGACCGCTATCCCGGACGCGCATCACTATTATCACGGTGAAAAAGTGGCATTCGGTACGCt < 1:768121/69‑1 (MQ=255) tAACGGCCTGACCGCTATCCCGGACGCGCATCACTATTATCACGGTGAAAAAGTGGCATTCGGTACGCt < 1:464163/69‑1 (MQ=255) tAACGGCCTGACCGCTATCCCGGACGCGCATCACTATTATCACGGTGAAAAAGTGGCATTCGGTACGCt < 1:139776/69‑1 (MQ=255) aCGGCCTGACCGCTATCCCGGACGCGCATCACTATTATCACGGTGAAAAAg < 1:1453860/51‑1 (MQ=255) aCGGCCTGACCGCTATCCCGGACGCGCATCACTATTATCACGGTGAAAAAg < 1:2710267/51‑1 (MQ=255) aCGGCCTGACCGCTATCCCGGACGCGCATCACTATTATCACGGTGAAAAAGTGGCATTCGGTACGCTg > 1:75110/1‑68 (MQ=255) aCGGCCTGACCGCTATCCCGGACGCGCATCACTATTATCACGGTGAAAAAGTGGCATTCGGTACGCTGa > 1:2976726/1‑69 (MQ=255) aCGGCCTGACCGCTATCCCGGACGCGCATCACTATTATCACGGTGAAAAAGTGGCATTCGGTACGCTGa > 1:320091/1‑69 (MQ=255) aCGGCCTGACCGCTATCCCGGACGCGCATCACTATTATCACGGTGAAAAAGTGGCATTCGGTACGCTGa > 1:3430618/1‑69 (MQ=255) aCGGCCTGACCGCTATCCCGGACGCGCATCACTATTATCACGGTGAAAAAGTGGCATTCGGTACGCTGa > 1:729848/1‑69 (MQ=255) aCGGCCTGACCGCTATCCCGGACGCGCATCACTATTATCACGGTGAAAAAGTGGCATTCGGTACGCTGa > 1:2340951/1‑69 (MQ=255) aCGGCCTGACCGCTATCCCGGACGCGCATCACTATTATCACGGTGAAAAAGTGGCATTCGGTACGCTGa > 1:2326992/1‑69 (MQ=255) aCGGCCTGACCGCTATCCCGGACGCGCATCACTATTATCACGGTGAAAAAGTGGCATTCGGTACGCTGa > 1:755466/1‑69 (MQ=255) aCGGCCTGACCGCTATCCCGGACGCGCATCACTATTATCACGGTGAAAAAGTGGCATTCGGTACGCTGa > 1:1226146/1‑69 (MQ=255) aCGGCCTGACCGCTATCCCGGACGCGCATCACTATTATCACGGTGAAAAAGTGGCATTCGGTACGCTGa > 1:939171/1‑69 (MQ=255) aCGGCCTGACCGCTATCCCGGACGCGCATCACTATTACCACGGTGAAAAAGTGGCATTCGGTACGCTGa > 1:2435295/1‑69 (MQ=255) ggCCTGACCGCTATCCCGGACGCGCATCACTATTATCACGGTGAAAAAGTGGCATTCGGTACGCTGa > 1:187125/1‑67 (MQ=255) ggCCTGACCGCTATCCCGGACGCGCATCACTATTATCACGGTGAAAAAGTGGCATTCGGTACGCTGa > 1:2355301/1‑67 (MQ=255) ggCCTGACCGCTATCCCGGACGCGCATCACTATTATCACGGTGAAAAAGTGGCATTCGGTACGCTGa > 1:895298/1‑67 (MQ=255) ggCCTGACCGCTATCCCGGACGCGCATCACTATTATCACGGTGAAAAAGTGGCATTCGGTACGCTGa > 1:3309599/1‑67 (MQ=255) ggCCTGACCGCTATCCCGGACGCGCATCACTATTATCACGGTGAAAAAGTGGCATTCGGTACGCTGa > 1:1017767/1‑67 (MQ=255) ccTGACCGCTATCCCGGACGCGCATCACTATTATCACGGTGAAAAAGTGGCa < 1:2012597/52‑1 (MQ=255) cTGACCGCTATCCCGGACGCGCATCACTATTATCACGGTGAAAAAGTGGCATTCGGTACGCTGACGCAg > 1:385594/1‑69 (MQ=255) cTGACCGCTATCCCGGACGCGCATCACTATTATCACGGTGAAAAAGTGGCATTCGGTACGCTGACGCAg > 1:2045247/1‑69 (MQ=255) cTGACCGCTATCCCGGACGCGCATCACTATTATCACGGTGAAAAAGTGGCATTCGGTACGCTGACGCAg > 1:2733429/1‑69 (MQ=255) cTGACCGCTATCCCGGACGCGCATCACTATTATCACGGTGAAAAAGTGGCATTCGGTACGCTGACGCAg > 1:1999765/1‑69 (MQ=255) cTGACCGCTATCCCGGACGCGCATCACTATTATCACGGTGAAAAAGTGGCATTCGGTACGCTGACGCAg > 1:2240493/1‑69 (MQ=255) cTGACCGCTATCCCGGACGCGCATCACTATTATCACGGTGAAAAAGTGGCATTCGGTACGCTGACGCAg > 1:1865279/1‑69 (MQ=255) ccGGACGCGCATCACTATTATCATGTTGAAAAAGTGGCATTCGGTACGCTGACGCAGc < 1:621326/58‑1 (MQ=255) ccGGACGCGCATCACTATTATCATGGTGAAAAAGTGGCATTCGGTACGCTGACGCAGc < 1:1410643/58‑1 (MQ=255) ccGGACGCGCATCACTATTATCATGGTGAAAAAGTGGCATTCGGTACGCTGACGCAGc < 1:400212/58‑1 (MQ=255) ccGGACGCGCATCACTATTATCATGGTGAAAAAGTGGCATTCGGTACGCTGACGCAGc < 1:452437/58‑1 (MQ=255) ccGGACGCGCATCACTATTATCATGGTGAAAAAGTGGCATTCGGTACGCTGACGCAGc < 1:1810043/58‑1 (MQ=255) ccGGACGCGCATCACTATTATCATGGTGAAAAAGTGGCATTCGGTACGCTGACGCAGc < 1:47475/58‑1 (MQ=255) ccGGACGCGCATCACTATTATCATGGTGAAAAAGTGGCATTCGGTACGCTGACGCAGc < 1:584187/58‑1 (MQ=255) ccGGACGCGCATCACTATTATCATGGTGAAAAAGTGGCATTCGGTACGCTGACGCAGc < 1:1447358/58‑1 (MQ=255) ccGGACGCGCATCACTATTATCATGGTGAAAAAGTGGCATTCGGTACGCTGACGCAGc < 1:2708133/58‑1 (MQ=255) ccGGACGCGCATCACTATTATCATGGTGAAAAAGTGGCATTCGGTACGCTGACGCAGc < 1:2376826/58‑1 (MQ=255) ccGGACGCGCATCACTATTATCATGGTGAAAAAGTGGCATTCGGTACGCTGACGCAGc < 1:232116/58‑1 (MQ=255) ccGGACGCGCATCACTATTATCATGGTGAAAAAGTGGCATTCGGTACGCTGACGCAGc < 1:886213/58‑1 (MQ=255) ccGGACGCGCATCACTATTATCATGGTGAAAAAGTGGCATTCGGTACGCTGACGCAGc < 1:213551/58‑1 (MQ=255) ccGGACGCGCATCACTATTATCATGGTGAAAAAGTGGCATTCGGTACGCTGACGCAGc < 1:2030819/58‑1 (MQ=255) ccGGACGCGCATCACTATTATCATGGTGAAAAAGTGGCATTCGGTACGCTGACGCAGc < 1:2765881/58‑1 (MQ=255) ccGGACGCGCATCACTATTATCATGGTGAAAAAGTGGCATTCGGTACGCTGACGCAGc < 1:2961456/58‑1 (MQ=255) ccGGACGCGCATCACTATTATCATGGTGAAAAAGTGGCATTCGGTACGCTGACGCAGc < 1:2864253/58‑1 (MQ=255) ccGGACGCGCATCACTATTATCATGGTGAAAAAGTGGCATTCGGTACGCTGACGCAGc < 1:1737243/58‑1 (MQ=255) ccGGACGCGCATCACTATTATCATGGTGAAAAAGTGGCATTCGGTACGCTGACGCAGc < 1:3009385/58‑1 (MQ=255) ccGGACGCGCATCACTATTATCATGGTGAAAAAGTGGCATTCGGTACGCTGACGCAGc < 1:301564/58‑1 (MQ=255) ccGGACGCGCATCACTATTATCATGGTGAAAAAGTGGCATTCGGTACGCTGACGCAGc < 1:3032030/58‑1 (MQ=255) ccGGACGCGCATCACTATTATCATGGTGAAAAAGTGGCATTCGGTACGCTGACGCAGc < 1:3054433/58‑1 (MQ=255) ccGGACGCGCATCACTATTATCATGGTGAAAAAGTGGCATTCGGTACGCTGACGCAGc < 1:3197494/58‑1 (MQ=255) ccGGACGCGCATCACTATTATCATGGTGAAAAAGTGGCATTCGGTACGCTGACGCAGc < 1:3261887/58‑1 (MQ=255) ccGGACGCGCATCACTATTATCATGGTGAAAAAGTGGCATTCGGTACGCTGACGCAGc < 1:1609282/58‑1 (MQ=255) ccGGACGCGCATCACTATTATCATGGTGAAAAAGTGGCATTCGGTACGCTGACGCAGc < 1:3365359/58‑1 (MQ=255) ccGGACGCGCATCACTATTATCATGGTGAAAAAGTGGCATTCGGTACGCTGACGCAGc < 1:3379600/58‑1 (MQ=255) cgcgCATCACTATTATCACGGTGAAAAAGTGGCATTCGGTACGCTGACGCAGCTGGTTCTGGAAAATgc > 1:1845985/1‑69 (MQ=255) gcATCACTATTATCACGGTGAAAAAGTGGCATTCGGTACGCTGACGCAGCTGGTTCTGGAAAATGCGcc > 1:2575490/1‑69 (MQ=255) gcATCACTATTATCACGGTGAAAAAGTGGCATTCGGTACGCTGACGCAGCTGGTTCTGGAAAATGCGcc > 1:1292590/1‑69 (MQ=255) gcATCACTATTATCACGGTGAAAAAGTGGCATTCGGTACGCTGACGCAGCTGGTTCTGGAAAATGCGcc > 1:2490517/1‑69 (MQ=255) gcATCACTATTATCACGGTGAAAAAGTGGCATTCGGTACGCTGACGCAGCTGGTTCTGGAAAATGCGcc > 1:568119/1‑69 (MQ=255) gcATCACTATTATCACGGTGAAAAAGTGGCATTCGGTACGCTGACGCAGCTGGTTCTGGAAAATGCGcc > 1:2730744/1‑69 (MQ=255) aCTATTATCACGGTGAAAAAGTGGCATTCGGTACGCTGACGCAGCTGGTTCTGGAAAATGCGCCGGTgg > 1:2824503/1‑69 (MQ=255) aCTATTATCACGGTGAAAAAGTGGCATTCGGTACGCTGACGCAGCTGGTTCTGGAAAATGCGCCGGTgg > 1:622900/1‑69 (MQ=255) aCTATTATCACGGTGAAAAAGTGGCATTCGGTACGCTGACGCAGCTGGTTCTGGAAAATGCGCCGGTgg > 1:802232/1‑69 (MQ=255) aCTATTATCACGGTGAAAAAGTGGCATTCGGTACGCTGACGCAGCTGGTTCTGGAAAATGCGCCGGTgg > 1:2005151/1‑69 (MQ=255) aCTATTATCACGGTGAAAAAGTGGCATTCGGTACGCTGACGCAGCTGGTTCTGGAAAATGCGCCGGTgg > 1:928383/1‑69 (MQ=255) | TGGCTGCGGCGCACGCAGTGCATAACGGCCTGACCGCTATCCCGGACGCGCATCACTATTATCACGGTGAAAAAGTGGCATTCGGTACGCTGACGCAGCTGGTTCTGGAAAATGCGCCGGTGG > minE/2293618‑2293740 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |