| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,296,088 | C→T | 48.0% | E661E (GAG→GAA) | katG ← | catalase/hydroperoxidase HPI(I) |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,296,088 | 0 | C | T | 48.0% | 3.8 / 66.2 | 77 | E661E (GAG→GAA) | katG | catalase/hydroperoxidase HPI(I) |
| Reads supporting (aligned to +/- strand): ref base C (16/24); new base T (19/18); total (35/42) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.65e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.95e-02 | |||||||||||
GGCTGGCCGTAAATTTCACTTCGCCGGTTTCACGGTCACGGCCTTCGAACAGCTCTTTCGATTCGTCGGTCGCTTTCCACTCGTAACGCATATCCAGCAAGTTCACGAAGAAGT > minE/2296036‑2296149 | ggctggctGTGTATTTCACTTCGCCAGTTTCGCGGTCACGGCCTTCGAACAGTTCTTTCGATTCgtcgg < 1:243021/69‑1 (MQ=255)ggctggctGTGTATTTCACTTCGCCAGTTTCGCGGTCACGGCCTTCGAACAGTTCTTTCGATTCgtcgg < 1:2844590/69‑1 (MQ=255)ggctggctGTGTATTTCACTTCGCCAGTTTCGCGGTCACGGCCTTCGAACAGTTCTTTCGATTCgtcgg < 1:185885/69‑1 (MQ=255)ggctggctGTGTATTTCACTTCGCCAGTTTCGCGGTCACGGCCTTCGAACAGTTCTTTCGATTCgtcgg < 1:347096/69‑1 (MQ=255)ggctggctGTGTATTTCACTTCGCCAGTTTCGCGGTCACGGCCTTCGAACAGTTCTTTCGATTCgtcgg < 1:1758063/69‑1 (MQ=255)ggctggctGTGTATTTCACTTCGCCAGTTTCGCGGTCACGGCCTTCGAACAGTTCTTTCGATTCgtcgg < 1:2357155/69‑1 (MQ=255)ggctggctGTGTATTTCACTTCGCCAGTTTCGCGGTCACGGCCTTCGAACAGTTCTTTCGATTCgtcgg < 1:1669907/69‑1 (MQ=255)ggctggctGTGTATTTCACTTCGCCAGTTTCGCGGTCACGGCCTTCGAACAGTTCTTTCGATTCgtcgg < 1:3366343/69‑1 (MQ=255)ggctggctGTGTATTTCACTTCGCCAGTTTCGCGGTCACGGCCTTCGAACAGTTCTTTCGATTCgtcgg < 1:2403989/69‑1 (MQ=255)ggctggctGTGTATTTCACTTCGCCAGTTTCGCGGTCACGGCCTTCGAACAGTTCTTTCGATTCgtcgg < 1:1394125/69‑1 (MQ=255)ggctggctGTGTATTTCACTTCGCCAGTTTCGCGGTCACGGCCTTCGAACAGTTCTTTCGATTCgtcgg < 1:748161/69‑1 (MQ=255)ggctggctGTGTATTTCACTTCGCCAGTTTCGCGGTCACGGCCTTCGAACAGTTCTTTCGATTCgtcgg < 1:748327/69‑1 (MQ=255)ggctggctGTGTATTTCACTTCGCCAGTTTCGCGGTCACGGCCTTCGAACAGTTCTTTCGATTCgtcgg < 1:2491526/69‑1 (MQ=255)ggctggctGTGTATTTCACTTCGCCAGTTTCGCGGTCACGGCCTTCGAACAGTTCTTTCGATTCgtcgg < 1:25868/69‑1 (MQ=255)ggctggctGTGTATTTCACTTCGCCAGTTTCGCGGTCACGGCCTTCGAACAGTTCTTTCGATTCgtcgg < 1:2682495/69‑1 (MQ=255)ggctggctGTGTATTTCACTTCGCCAGTTTCGCGGTCACGGCCTTCGAACAGTTCTTCCGATTCgtcgg < 1:1453774/69‑1 (MQ=255)agctggctgTGTATTTCACTTCGCCAGTTTCGCGGTCACGGCCTTCGAACAGTTCTTTCGATTCgtcgg < 1:1851481/68‑1 (MQ=255) gctggctgTGTATTTCACTTCGCCAGTTTCGCGGTCACGGCCTTCGAACAGTTCTTTCGATTCgtcgg < 1:500323/68‑1 (MQ=255) gTAAATTTCACTTCGCCGGTTTCACGGTCACGGCCTTCGAACAGCTCTTTCGATTCGTCGGTCGCTTTc < 1:2455323/69‑1 (MQ=255) gTAAATTTCACTTCGCCGGTTTCACGGTCACGGCCTTCGAACAGCTCTTTCGATTCGTCGGTCGCTTTc < 1:841872/69‑1 (MQ=255) aaaTTTCACTTCGCCGGTTTCACGGTCACGGCCTTCGAACAGCTCTTTCGATTCGTCGGTCGCTTTCCa < 1:3193976/69‑1 (MQ=255) aaTTTCACTTCGCCGGTTTCACGGTCACGGCCTTCGAACAGCTCTTTCGATTCGTCGGTCGCTTTCCAc > 1:2905066/1‑69 (MQ=255) aaTTTCACTTCGCCGGTTTCACGGTCACGGCCTTCGAACAGCTCTTTCGATTCGTCGGTCGCTTTCCAc > 1:2685723/1‑69 (MQ=255) aaTTTCACTTCGCCGGTTTCACGGTCACGGCCTTCGAACAGCTCTTTCGATTCGTCGGTCGCTTTCCAc > 1:2882285/1‑69 (MQ=255) aaTTTCACTTCGCCGGTTTCACGGTCACGGCCTTCGAACAGCTCTTTCGATTCGTCGGTCGCTTTCCAc > 1:1703803/1‑69 (MQ=255) aaTTTCACTTCGCCGGTTTCACGGTCACGGCCTTCGAACAGCTCTTTCGATTCGTCGGTCGCTTTCCAc > 1:1577549/1‑69 (MQ=255) aaTTTCACTTCGCCGGTTTCACGGTCACGGCCTTCGAACAGCTCTTTCGATTCGTCGGTCGCTTTCCAc > 1:649113/1‑69 (MQ=255) aaTTTCACTTCGCCGGTTTCACGGTCACGGCCTTCGAACAGCTCTTTCGATTCGTCGGTCGCTTTCCAc > 1:699056/1‑69 (MQ=255) aaTTTCACTTCGCCGGTTTCACGGTCACGGCCTTCGAACAGCTCTTTCGATTCGTCGGTCGCTTTCCAc > 1:1343683/1‑69 (MQ=255) aaTATCACTTCGCCGGTTTCACGGTCACGGCCTTCGAACAGCTCTTTCGATTCGTCGGTCGCTTTc > 1:2836257/1‑66 (MQ=255) ttCGCCGGTTTCACGGTCACGGCCTTCGAACAGCTCTTTCGATTCGTCGGTCGCTTTCCACTCGTAACg < 1:1145158/69‑1 (MQ=255) ttCGCCGGTTTCACGGTCACGGCCTTCGAACAGCTCTTTCGATTCGTCGGTCGCTTTCCACTCGTAACg < 1:3034661/69‑1 (MQ=255) ttCGCCGGTTTCACGGTCACGGCCTTCGAACAGCTCTTTCGATTCGTCGGTCGCTTTCCACTCGTAACg < 1:903574/69‑1 (MQ=255) ccGGTTTCACGGTCACGGCCTTCGAACAGCTCTTTCGATTCGTCGGTCGCTTTCCACTCGTAACGCa < 1:501727/67‑1 (MQ=255) ccGGTTTCACGGTCACGGCCTTCGAACAGCTCTTTCGATTCGTCGGTCGCTTTCCACTCGTAACGCa < 1:280381/67‑1 (MQ=255) ccGGTTTCACGGTCACGGCCTTCGAACAGCTCTTTCGATTCGTCGGTCGCTTTCCACTCGTAACGCa < 1:2979934/67‑1 (MQ=255) ttCACGGTCACGGCCTTCGAACAGCTCTTTCGATTCGTCGGTCGCTTTCCACTCGTAACGCATATCCa > 1:2683138/1‑68 (MQ=255) ttCACGGTCACGGCCTTCGAACAGCTCTTTCGATTCGTCGGTCGCTTTCCACTCGTAACGCATATCCa > 1:2686994/1‑68 (MQ=255) tcgcggtcacggCCTTCGACCAGTTCTTTCGATTCGTCGGTCGCTTTCCACTCGTATCTCATATCCa > 1:1313494/4‑67 (MQ=255) tcgcggtcacggCCTTCGAACAGTTCTTTCGATTCGTCGGTCGCTTTCCACTCGTAACTCATATGCa > 1:447977/4‑67 (MQ=255) tcgcggtcacggCCTTCGAACAGTTCTTTCGATTCGTCGGTCGCTTTCCACTCGTAACTCATATCCa > 1:2163641/4‑67 (MQ=255) tcgcggtcacggCCTTCGAACAGTTCTTTCGATTCGTCGGTCGCTTTCCACTCGTAACTCATATCCa > 1:3180481/4‑67 (MQ=255) tcgcggtcacggCCTTCGAACAGTTCTTTCGATTCGTCGGTCGCTTTCCACTCGTAACTCATATCCa > 1:2528658/4‑67 (MQ=255) tcgcggtcacggCCTTCGAACAGTTCTTTCGATTCGTCGGTCGCTTTCCACTCGTAACTCATATCCa > 1:3368796/4‑67 (MQ=255) tcgcggtcacggCCTTCGAACAGTTCTTTCGATTCGTCGGTCGCTTTCCACTCGTAACTCATATCCa > 1:2080417/4‑67 (MQ=255) tcgcggtcacggCCTTCGAACAGTTCTTTCGATTCGTCGGTCGCTTTCCACTCGTAACTCATATCCa > 1:471745/4‑67 (MQ=255) tcgcggtcacggCCTTCGAACAGTTCTTTCGATTCGTCGGTCGCTTTCCACTCGTAACTCATATCCa > 1:1205162/4‑67 (MQ=255) tcgcggtcacggCCTTCGAACAGTTCTTTCGATTCGTCGGTCGCTTTCCACTCGTAACTCATATCCa > 1:1001910/4‑67 (MQ=255) tcgcggtcacggCCTTCGAACAGTTCTTTCGATTCGTCGGTCGCTTTCCACTCGTAACGCATATCCa > 1:3189737/4‑67 (MQ=255) tcgcggtcacggCCTTCGAACAGTTCTTTCGATTCGTCGGTCGCTTTCCACTCGTAACGCATATCCa > 1:1024511/4‑67 (MQ=255) tcgcggtcacggCCTTCGAACAGTTCTTTCGATTCGTCGGTCGCTTTCCACTCGTAACGCATATCCa > 1:288540/4‑67 (MQ=255) tcgcggtcacggCCTTCGAACAGTTCTTTCGATTCGTCGGTCGCTTTCCACTCGTAACGCATATCCa > 1:132886/4‑67 (MQ=255) tcgcggtcacggCCTTCGAACAGTTCTTTCGATTCGTCGGTCGCTTTCCACTCGTAACGCATATCCa > 1:2670251/4‑67 (MQ=255) tcgcggtcacggCCTTCGAACAGTTCTTTCGATTCGTCGGTCGCTTTCCACTCGTAACGCATATCCa > 1:2577386/4‑67 (MQ=255) tcgcggtcacggCCTTCGAACAGTTCTTTCGATTCGTCGGTCGCTTTCCACTCGTAACGCATATCCa > 1:2401837/4‑67 (MQ=255) tcgcggtcacggCCTTCGAACAGTTCTTTCGATTCGTCGGTCGCTTTCCACTCGTAACGCATATCCa > 1:2229925/4‑67 (MQ=255) tcgcggtcacggCCTTCGAACAGTTCTTTCGATTCGTCGGACGCTTTCCACTCGTAACGCATATCCa > 1:298009/4‑67 (MQ=255) aaCAGCTCTTTCGATTCGTCGGTCGCTTTCCACTCGTAACGCATATCCAGCAAGTTCACGAAGAAGt < 1:989272/67‑1 (MQ=255) aaCAGCTCTTTCGATTCGTCGGTCGCTTTCCACTCGTAACGCATATCCAGCAAGTTCACGAAGAAGt < 1:963460/67‑1 (MQ=255) aaCAGCTCTTTCGATTCGTCGGTCGCTTTCCACTCGTAACGCATATCCAGCAAGTTCACGAAGAAGt < 1:1274872/67‑1 (MQ=255) aaCAGCTCTTTCGATTCGTCGGTCGCTTTCCACTCGTAACGCATATCCAGCAAGTTCACGAAGAAGt < 1:1451223/67‑1 (MQ=255) aaCAGCTCTTTCGATTCGTCGGTCGCTTTCCACTCGTAACGCATATCCAGCAAGTTCACGAAGAAGt < 1:1838977/67‑1 (MQ=255) aaCAGCTCTTTCGATTCGTCGGTCGCTTTCCACTCGTAACGCATATCCAGCAAGTTCACGAAGAAGt < 1:1947280/67‑1 (MQ=255) aaCAGCTCTTTCGATTCGTCGGTCGCTTTCCACTCGTAACGCATATCCAGCAAGTTCACGAAGAAGt < 1:2033042/67‑1 (MQ=255) aaCAGCTCTTTCGATTCGTCGGTCGCTTTCCACTCGTAACGCATATCCAGCAAGTTCACGAAGAAGt < 1:2120422/67‑1 (MQ=255) aaCAGCTCTTTCGATTCGTCGGTCGCTTTCCACTCGTAACGCATATCCAGCAAGTTCACGAAGAAGt < 1:2306004/67‑1 (MQ=255) aaCAGCTCTTTCGATTCGTCGGTCGCTTTCCACTCGTAACGCATATCCAGCAAGTTCACGAAGAAGt < 1:2639859/67‑1 (MQ=255) aaCAGCTCTTTCGATTCGTCGGTCGCTTTCCACTCGTAACGCATATCCAGCAAGTTCACGAAGAAGt < 1:3163485/67‑1 (MQ=255) aaCAGCTCTTTCGATTCGTCGGTCGCTTTCCACTCGTAACGCATATCCAGCAAGTTCACGAAGAAGt < 1:3078595/67‑1 (MQ=255) aaCAGCTCTTTCGATTCGTCGGTCGCTTTCCACTCGTAACGCATATCCAGCAAGTTCACGAAGAAGt < 1:2982339/67‑1 (MQ=255) aaCAGCTCTTTCGATTCGTCGATCGCTTTCCACTCGTAACGCATATCCAGCAAGTTCACGAAGAAGt < 1:2761512/67‑1 (MQ=255) aCAGCTCTTTCGATTCGGCGGTCGCTTTCCACTCGTAACGCa < 1:3278593/42‑1 (MQ=255) gCTCTTTCGATTCGTCGGTCGCTTTCCACTCGTAACGCATATCCAGCa > 1:416745/1‑48 (MQ=255) gCTCTTTCGATTCGTCGGTCGCTTTCCACTCGTAACGCATATCCAGCa > 1:576221/1‑48 (MQ=255) gCTCTTTCGATTCGTCGGTCGCTTTCCACTCGTAACGCATATCCAGCa > 1:1532995/1‑48 (MQ=255) gCTCTTTCGATTCGTCGGTCGCTTTCCACTCGTAACGCATATCCAGCa > 1:1344696/1‑48 (MQ=255) gCTCTTTCGATTCGTCGGTCGCTTTCCACTCGTAACGCATATCCAGCa > 1:119679/1‑48 (MQ=255) | GGCTGGCCGTAAATTTCACTTCGCCGGTTTCACGGTCACGGCCTTCGAACAGCTCTTTCGATTCGTCGGTCGCTTTCCACTCGTAACGCATATCCAGCAAGTTCACGAAGAAGT > minE/2296036‑2296149 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |