| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,301,381 | G→A | 37.8% | S230S (AGC→AGT) | metL ← | fused aspartokinase II and homoserine dehydrogenase II |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,301,381 | 0 | G | A | 37.8% | 36.5 / 39.9 | 66 | S230S (AGC→AGT) | metL | fused aspartokinase II and homoserine dehydrogenase II |
| Reads supporting (aligned to +/- strand): ref base G (30/11); new base A (14/11); total (44/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.84e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AACGGCAGCAGGCAGGCATCTTTCACTTTACGCGGGTCGGCACTGTATACCCCGGCGACGTCGCTCCAGATGGTTACGCGAGAAACACCCGCCAGCGCACCGATTTGTGTCGCGGAATAGTCGGAACCGT > minE/2301319‑2301448 | aaCGGCAGCAGGCAGGCATCTTTCACTTTACGCGGGTCGGCACTGTATACCCCGGCGACGTCGCTCCAg > 1:1409557/1‑69 (MQ=255)aaCGGCAGCAGGCAGGCATCTTTCACTTTACGCGGGTCGGCACTGTATACCCCGGCGACGTCGCTCCAg > 1:905144/1‑69 (MQ=255)aaCGGCAGCAGGCAGGCATCTTTCACTTTACGCGGGTCGGCACTGTATACCCCGGCGACGTCGCTCCAg > 1:560808/1‑69 (MQ=255)aaCGGCAGCAGGCAGGCATCTTTCACTTTACGCGGGTCGGCACTGTATACCCCGGCGACGTCGCTCCAg > 1:2897768/1‑69 (MQ=255)aaCGGCAGCAGGCAGGCATCTTTCACTTTACGCGGGTCGGCACTGTATACCCCGGCGACGTCGCTCCAg > 1:1946340/1‑69 (MQ=255)aaCGGCAGCAGGCAGGCATCTTTCACTTTACGCGGGTCGGCACTGTATACCCCGGCGACGTCGCTCCAg > 1:1322447/1‑69 (MQ=255)aaCGGCAGCAGGCAGGCATCTTTCACTTTACGCGGGTCGGCACTGTATACCCCGGCGACGTCGCTCCAg > 1:1544740/1‑69 (MQ=255)aaCGGCAGCAGGCAGGCATCTTTCACTTTACGCGGGTCGGCACTGTATACCCCGGCGACGTCGCTCCAg > 1:1284631/1‑69 (MQ=255)aaCGGCAGCAGGCAGGCATCTTTCACTTTACGCGGGTCGGCACTGTATACCCCGGCGACGTCGCTCCAg > 1:1310821/1‑69 (MQ=255) aTCTTTCACTTTACGCGGGTCGGCACTGTATACCCCGGCGACGTCGCTCCAGATGGTTACGCgaga < 1:368714/66‑1 (MQ=255) aTCTTTCACTTTACGCGGGTCGGCACTGTATACCCCGGCGACGTCGCTCCAGATGGTTACGCgaga < 1:1474981/66‑1 (MQ=255) aTCTTTCACTTTACGCGGGTCGGCACTGTATACCCCGGCGACGTCGCTCCAGATGGTTACGCgaga < 1:3316026/66‑1 (MQ=255) aTCTTTCACTTTACGCGGGTCGGCACTGTATACCCCGGCGACGTCGCTCCAGATGGTTACGCgaga < 1:114602/66‑1 (MQ=255) aTCTTTCACTTTACGCGGGTCGGCACTGTATACCCCGGCGACGTCGCTCCAGATGGTTACGCgaga < 1:2056318/66‑1 (MQ=255) aTCTTTCACTTTACGCGGGTCGGCACTGTATACCCCGGCGACGTCGCTCCAGATGGTTACGCgaga < 1:2433075/66‑1 (MQ=255) aTCTTTCACTTTACGCGGGTCGGCACTGTATACCCCGGCGACGTCGCTCCAGATGGTTACGCgaga < 1:2560977/66‑1 (MQ=255) tttCACTTTACGCGGGTCGGCACTGTATACCCCGGCGACGTCGCTCCAGATGGTTACGCGAGAAACAcc > 1:2671538/1‑69 (MQ=255) tttCACTTTACGCGGGTCGGCACTGTATACCCCGGCGACGTCGCTCCAGATGGTTACGCGAGAAACAcc > 1:1249806/1‑69 (MQ=255) cgGGTCGGCGCTATATACCCCGGCGACGTCACTCCAAATGGTTACGCGAGAAACACCCGCCAGCGCAcc > 1:2293764/1‑69 (MQ=255) cgGGTCGGCGCTATATACCCCGGCGACGTCACTCCAAATGGTTACGCGAGAAACACCCGCCAGCGCAcc > 1:1876609/1‑69 (MQ=255) cgGGTCGGCGCTATATACCCCGGCGACGTCACTCCAAATGGTTACGCGAGAAACACCCGCCAGCGCAcc > 1:2537776/1‑69 (MQ=255) cgGGTCGGCGCTATATACCCCGGCGACGTCACTCCAAATGGTTACGCGAGAAACACCCGCCAGCGCAcc > 1:2403478/1‑69 (MQ=255) cgGGTCGGCGCTATATACCCCGGCGACGTCACTCCAAATGGTTACGCGAGAAACACCCGCCAGCGCAcc > 1:2862053/1‑69 (MQ=255) cgGGTCGGCGCTATATACCCCGGCGACGTCACTCCAAATGGTTACGCGAGAAACACCCGCCAGCGCAcc > 1:1806371/1‑69 (MQ=255) cgGGTCGGCGCTATATACCCCGGCGACGTCACTCCAAATGGTTACGCGAGAAACACCCGCCAGCGCAcc > 1:316225/1‑69 (MQ=255) cgGGTCGGCGCTATATACCCCGGCGACGTCACTCCAAATGGTTACGCGAGAAACACCCGCCAGCGCAcc > 1:1081267/1‑69 (MQ=255) cgGGTCGGCGCTATATACCCCGGCGACGTCACTCCAAATGGTTACGCGAGAAACACCCGCCAGCGCAcc > 1:1037018/1‑69 (MQ=255) cGGCGCTATATACCCCGGCGACGTCACTCCAAATGGTTACGCGAGAAACACCCGCCAGCGCACCGAttt < 1:363356/69‑1 (MQ=255) cGGCGCTATATACCCCGGCGACGTCACTCCAAATGGTTACGCGAGAAACACCCGCCAGCGCACCGAttt < 1:471174/69‑1 (MQ=255) cGGCGCTATATACCCCGGCGACGTCACTCCAAATGGTTACGCGAGAAACACCCGCCAGCGCACCGAttt < 1:555310/69‑1 (MQ=255) cGGCGCTATATACCCCGGCGACGTCACTCCAAATGGTTACGCGAGAAACACCCGCCAGCGCACCGAttt < 1:960774/69‑1 (MQ=255) cGGCGCTATATACCCCGGCGACGTCACTCCAAATGGTTACGCGAGAAACACCCGCCAGCGCACCGAttt < 1:1132467/69‑1 (MQ=255) cGGCGCTATATACCCCGGCGACGTCACTCCAAATGGTTACGCGAGAAACACCCGCCAGCGCACCGAttt < 1:1139438/69‑1 (MQ=255) cGGCGCTATATACCCCGGCGACGTCACTCCAAATGGTTACGCGAGAAACACCCGCCAGCGCACCGAttt < 1:1357382/69‑1 (MQ=255) cGGCGCTATATACCCCGGCGACGTCACTCCAAATGGTTACGCGAGAAACACCCGCCAGCGCACCGAttt < 1:1395086/69‑1 (MQ=255) cGGCGCTATATACCCCGGCGACGCCACTCCAAATGGTTACGCGAGAAACACCCGCCAGCGCACCGAttt < 1:1820038/69‑1 (MQ=255) ggCGCTATATACCCCGGCGACGTAACTCCAAATGGTTACGCGAGAAACACCCGCCAGCGCACCGAttt < 1:2964449/68‑1 (MQ=255) ggCACTGTATACCCCGGCGACGTCGCTCCAGATGGTTACGCgaga < 1:3430247/45‑1 (MQ=255) ggCACTGTATACCCCGGCGACGTCGCTCCAGATGGTTACGCGAGAAACACCCGCCAGCGCACCGAttt > 1:1247251/1‑68 (MQ=255) ggCACTGTATACCCCGGCGACGTCGCTCCAGATGGTTACGCGAGAAACACCCGCCAGCGCACCGATTtg > 1:1062728/1‑69 (MQ=255) ggCACTGTATACCCCGGCGACGTCGCTCCAGATGGTTACGCGAGAAACACCCGCCAGCGCACCGATTtg > 1:613260/1‑69 (MQ=255) ggCACTGTATACCCCGGCGACGTCGCTCCAGATGGTTACGCGAGAAACACCCGCCAGCGCACCGATTtg > 1:1886709/1‑69 (MQ=255) ggCACTGTATACCCCGGCGACGTCGCTCCAGATGGTTACGCGAGAAACACCCGCCAGCGCACCGATTtg > 1:2311460/1‑69 (MQ=255) ggCACTGTATACCCCGGCGACGTCGCTCCAGATGGTTACGCGAGAAACACCCGCCAGCGCACCGATTtg > 1:1790112/1‑69 (MQ=255) ggCACTGTATACCCCGGCGACGTCGCTCCAGATGGTTACGCGAGAAACACCC‑CCAGCGCACCGATTtgt > 1:773366/1‑69 (MQ=255) gTATACCCCGGCGACGTCGCTCCAGATGGTTACGCGAGAAACACCCGCCAGCGCACCGATTTGTGTcg > 1:349446/1‑68 (MQ=255) gTATACCCCGGCGACGTCGCTCCAGATGGTTACGCGAGAAACACCCGCCAGCGCACCGATTTGTGTcg > 1:187046/1‑68 (MQ=255) gTATACCCCGGCGACGTCGCTCCAGATGGTTACGCGAGAAACACCCGCCAGCGCACCGATTTGTGTcg > 1:2127582/1‑68 (MQ=255) gTATACCCCGGCGACGTCGCTCCAGATGGTTACGCGAGAAACACCCGCCAGCGCACCGATTTGTGTcg > 1:2654841/1‑68 (MQ=255) gTATACCCCGGCGACGTCGCTCCAGATGGTTACGCGAGAAACACCCGCCAGCGCACCGATTTGTGTcg > 1:2751045/1‑68 (MQ=255) ataCCCCGGCGACGTCACTCCAAATGGTTACGCGAGAAaca > 1:601731/1‑41 (MQ=255) ataCCCCGGCGACGTCACTCCAAATGGTTACGCGAGAAACACCCGCCAGCGCACCGATTTGTGTCGCgg > 1:405599/1‑69 (MQ=255) ataCCCCGGCGACGTCACTCCAAATGGTTACGCGAGAAACACCCGCCAGCGCACCGATTTGTGTCGCgg > 1:811752/1‑69 (MQ=255) ataCCCCGGCGACGTCACTCCAAATGGTTACGCGAGAAACACCCGCCAGCGCACCGATTTGTGTCGCgg > 1:1102316/1‑69 (MQ=255) ataCCCCGGCGACGTCACTCCAAATGGTTACGCGAGAAACACCCGCCAGCGCACCGATTTGTGTCGCgg > 1:93398/1‑69 (MQ=255) gACGTCGCTCCAGATGGTTACGCGAGAAACACCCGCCAGCGCACCGATTTGTGTCGCGGAATAGTCGGa > 1:2086835/1‑69 (MQ=255) gACGTCGCTCCAGATGGTTACGCGAGAAACACCCGCCAGCGCACCGATTTGTGTCGCGGAATAGTCGGa > 1:2469621/1‑69 (MQ=255) gACGTCGCTCCAGATGGTTACGCGAGAAACACCCGCCAGCGCACCGATTTGTGTCGCGGAATAGTCGGa > 1:2452164/1‑69 (MQ=255) gACGTCGCTCCAGATGGTTACGCGAGAAACACCCGCCAGCGCACCGATTTGTGTCGCGGAATAGTCGGa > 1:88065/1‑69 (MQ=255) gACGTCGCTCCAGATGGTTACGCGAGAAACACCCGCCAGCGCACCGATTTGTGTCGCGGAATAGTCGGa > 1:3217258/1‑69 (MQ=255) aCGTCACTCCAAATGGTTACGCGAGAAACACCCGCCAGCGCACCGAttt < 1:1133108/49‑1 (MQ=39) tCGCTCCAGATGGTTACGCGAGAAACACCCGCCAGCGCACCGATTTGTGTCGCGGAATAGTCGGAAcc > 1:212154/1‑68 (MQ=255) tCGCTCCAGATGGTTACGCGAGAAACACCCGACAGCGCACCGATTTGTGTCGCGGAATAGTCGGAAcc > 1:792087/1‑68 (MQ=255) cGCTCCAGATGGTTACGCGAGAAACACCCGCCAGCGCACCGATTTGTGTCGCGGAATAGTCGGAACCGt < 1:1920577/69‑1 (MQ=255) cGCTCCAGATGGTTACGCGAGAAACACCCGCCAGCGCACCGATTTGTGTCGCGGAATAGTCGGAACCGt < 1:374549/69‑1 (MQ=255) cGCTCCAGATGGTTACGCGAGAAACACCCGCCAGCGCACCGATTTGTGTCGCGGAATAGTCGGAACCGt < 1:41085/69‑1 (MQ=255) gCTCCAGATGGTTACGCGAGAAACACCCGCCAGCGCACCGATTTGTGTCGCGGAATAGTCGGAACCGt < 1:271457/68‑1 (MQ=255) | AACGGCAGCAGGCAGGCATCTTTCACTTTACGCGGGTCGGCACTGTATACCCCGGCGACGTCGCTCCAGATGGTTACGCGAGAAACACCCGCCAGCGCACCGATTTGTGTCGCGGAATAGTCGGAACCGT > minE/2301319‑2301448 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |