| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,301,495 | T→C | 61.9% | G192G (GGA→GGG) | metL ← | fused aspartokinase II and homoserine dehydrogenase II |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,301,495 | 0 | T | C | 61.9% | 27.8 / 33.2 | 55 | G192G (GGA→GGG) | metL | fused aspartokinase II and homoserine dehydrogenase II |
| Reads supporting (aligned to +/- strand): ref base T (10/11); new base C (9/25); total (19/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.47e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01 | |||||||||||
GGAATAGTCGGAACCGTTACGCCCCAGCAGCACCGTTTCACCGGCGTTGTTGCGGCTGATAAATCCGGTCACCACCAGACGTTTGCCCGGATGTTGCACCAGCAGCTGTTGCAGCAACGGGTAA > minE/2301432‑2301555 | ggAATAGTCGGAACCGTTACGCCCCAGCAGCACCGTTTCACCGGCGTTGTTGCGGCTGATAAATCCGGt < 1:2074378/69‑1 (MQ=255)ggAATAGTCGGAACCGTTACGCCCCAGCAGCACCGTTTCACCGGCGTTGTTGCGGCTGATAAATCCGGt < 1:1549309/69‑1 (MQ=255) gAATAGTCGGAACCGTTACGCCCCAGCAGCACCGTTTCACCGGCGTTGTTGCGGCTGATAAATCCGGt < 1:1237719/68‑1 (MQ=255) aTAGTCGGAACCGTTACGCCCCAGCAGCACCGTTTCACCGGCGTTGTTGCGGCTGATAAATCCGGTcac < 1:367138/69‑1 (MQ=255) tAGTCGGAACCGTTACGCCCAAGCAGCACCGTTTCACCGGCGTTGTTACGGCTGATAAACCCGGTcac < 1:1524888/68‑1 (MQ=38) tAGTCGGAACCGTTACGCCCAAGCAGCACCGTTTCACCGGCGTTGTTACGGCTGATAAACCCGGTcac < 1:2163715/68‑1 (MQ=38) tAGTCGGAACCGTTACGCCCAAGCAGCACCGTTTCACCGGCGTTGTTACGGCTGATAAACCCGGTcac < 1:1948303/68‑1 (MQ=38) tAGTCGGAACCGTTACGCCCAAGCAGCACCGTTTCACCGGCGTTGTTACGGCTGATAAACCCGGTcac < 1:3023791/68‑1 (MQ=38) tAGTCGGAACCGTTACGCCCAAGCAGCACCGTTTCACCGGCGTTGTTACGGCTGATAAACCCGGTcac < 1:3092698/68‑1 (MQ=38) tAGTCGGAACCGTTACGCCCAAGCAGCACCGTTTCACCGGCGTTGTTACGGCTGATAAACCCGGTcac < 1:3161553/68‑1 (MQ=38) tAGTCGGAACCGTTACGCCCAAGCAGCACCGTTTCACCGGCGTTGTTACGGCTGATAAACCCGGTcac < 1:1800795/68‑1 (MQ=38) tAGTCGGAACCGTTACGCCCAAGCAGCACCGTTTCACCGGCGTTGTTACGGCTGATAAACCCGGTcac < 1:1759019/68‑1 (MQ=38) tAGTCGGAACCGTTACGCCCAAGCAGCACCGTTTCACCGGCGTTGTTACGGCTGATAAACCCGGTcac < 1:3292625/68‑1 (MQ=38) tAGTCGGAACCGTTACGCCCAAGCAGCACCGTTTCACCGGCGTTGTTACGGCTGATAAACCCGGTcac < 1:1718241/68‑1 (MQ=38) tAGTCGGAACCGTTACGCCCAAGCAGCACCGTTTCACCGGCGTTGTTACGGCTGATAAACCCGGTcac < 1:38958/68‑1 (MQ=38) tAGTCGGAACCGTTACGCCCAAGCAGCACCGTTTCACCGGCGTTGTTACGGCTGATAAACCCGGTcac < 1:1596430/68‑1 (MQ=38) tAGTCGGAACCGTTACGCCCAAGCAGCACCGTTTCACCGGCGTTGTTACGGCTGATAAACCCGGTcac < 1:147971/68‑1 (MQ=38) tAGTCGGAACCGTTACGCCCAAGCAGCACCGTTTCACCGGCGTTGTTACGGCTGATAAACCCGGTcac < 1:1468092/68‑1 (MQ=38) tAGTCGGAACCGTTACGCCCAAGCAGCACCGTTTCACCGGCGTTGTTACGGCTGATAAACCCGGTcac < 1:412468/68‑1 (MQ=38) tAGTCGGAACCGTTACGCCCAAGCAGCACCGTTTCACCGGCGTTGTTACGGCTGATAAACCCGGTcac < 1:603332/68‑1 (MQ=38) tAGTCGGAACCGTTACGCCCAAGCAGCACCGTTTCACCGGCGTTGTTACGGCTGATAAACCCGGTcac < 1:1367037/68‑1 (MQ=38) tAGTCGGAACCGTTACGCCCAAGCAGCACCGTTTCACCGGCGTTGTTACGGCTGATAAACCCGGTcac < 1:1276796/68‑1 (MQ=38) tAGTCGGAACCGTTACGCCCAAGCAGCACCGTTTCACCGGCGTTGTTACGGCTGATAAACCCGGTcac < 1:1197532/68‑1 (MQ=38) tAGTCGGAACCGTTACGCCCAAGCAGCACCGTTTCACCGGCGTTGTTACGGCTGATAAACCCGGTcac < 1:1160015/68‑1 (MQ=38) tAGTCGGAACCGTTACGCCCAAGCAGCACCGTTTCACCGGCGTTGTTACGGCTGATAAACCCGGTcac < 1:627549/68‑1 (MQ=38) tAGTCGGAACCGTTACGCCCAAGCAGCACCGTTTCACCGGCGTTGTTACGGCTGATAAACCCGGTcac < 1:949219/68‑1 (MQ=38) tAGTCGGAACCGTTACGCCCAAGCAGCACCGTTTCACCGGCGTTGTTACGGCTGATAAACCCGGTcac < 1:1033338/68‑1 (MQ=38) aGTCGGAACCGTTACGCCCAAGCAGCACCGTTTCACCGGCGTTGTTACGGCTGATAAACCCGGTcac < 1:1120727/67‑1 (MQ=38) aCCGTTACGCCCCAGCAGCACCGTTTCACCGGCGTTGTTGCGGCTGATAAATCCGGTCACCACCAGACg < 1:38998/69‑1 (MQ=255) aCCGTTACGCCCCAGCAGCACCGTTTCACCGGCGTTGTTGCGGCTGATAAATCCGGTCACCACCAGACg < 1:279600/69‑1 (MQ=255) cccAGCAGCACCGTTTCACCGGCGTTGTTGCGGCTGATAAATCCGGTCACCACCAGACGTTTGCCCGGa < 1:1080236/69‑1 (MQ=255) cccAGCAGCACCGTTTCACCGGCGTTGTTGCGGCTGATAAATCCGGTCACCACCAGACGTTTGCCCGGa < 1:1370139/69‑1 (MQ=255) cccAGCAGCACCGTTTCACCGGCGTTGTTGCGGCTGATAAATCCGGTCACCACCAGACGTTTGCCCGGa < 1:137332/69‑1 (MQ=255) cccAGCAGCACCGTTTCACCGGCGTTGTTGCGGCTGATAAATCCGGTCACCACCAGACGTTTGCCCGGa < 1:1411193/69‑1 (MQ=255) cagcaCCGTTTCACCGGCGTTGTTGCGGCTGATAAATCCGGTCACCACCAGACGTTTGCCCGGATGTTg < 1:2933905/69‑1 (MQ=255) gTTTCACCGGCGTTGTTGCGGCTGATAAATCCGGTCACCACCAGACGTTTGCCCGGATGTTGCACcagc > 1:2764215/1‑69 (MQ=255) tCACCGGCGTTGTTGCGGCTGATAAATCCGGTCACCACCAGACGTTTGCCCGGATGTTGCACCagcagc > 1:1866413/1‑69 (MQ=255) tCACCGGCGTTGTTGCGGCTGATAAATCCGGTCACCACCAGACGTTTGCCCGGATGTTGCACCagcagc > 1:66095/1‑69 (MQ=255) tCACCGGCGTTGTTGCGGCTGATAAATCCGGTCACCACCAGACGTTTGCCCGGATGTTGCACCagcagc > 1:1701493/1‑69 (MQ=255) tCACCGGCGTTGTTGCGGCTGATAAATCCGGTCACCACCAGACGTTTGCCCGGATGTTGCACCagcagc > 1:849182/1‑69 (MQ=255) tCACCGGCGTTGTTGCGGCTGATAAATCCGGTCACCACCAGACGTTTGCCCGGATGTTGCACCagcagc > 1:1749173/1‑69 (MQ=255) tCACCGGCGTTGTTGCGGCTGATAAATCCGGTCACCACCAGACGTTTGCCCGGATGTTGCACCagcagc > 1:890958/1‑69 (MQ=255) tCACCGGCGTTGTTGCGGCTGATAAATCCGGTCACCACCAGACGTTTGCCCGGATGTTGCACCagcagc > 1:1926906/1‑69 (MQ=255) tCACCGGCGTTGTTGCGGCTGATAAATCCGGTCACCACCAGACGTTTGCCCGGATGTTGCACCagcagc > 1:2623304/1‑69 (MQ=255) tCACCGGCGTTGTTGCGGCTGATAAATCCGGTCACCACCAGACGTTT‑CCCGGATGTTGCACCAGCAGCt > 1:183073/1‑69 (MQ=255) tCACCGGCGTTGTTACGGCTGATAAACCCGGTcac < 1:2356031/35‑1 (MQ=37) tGATAAACCCGGTCACCACCAGCCGTTTGCCCGGAt > 1:2782110/1‑36 (MQ=21) tGATAAACCCGGTCACCACCAGACGTTTGCCCGGATGTTGTACCAGTAGCTGTTGCAGCAACGGGTac > 1:1089442/1‑67 (MQ=255) tGATAAACCCGGTCACCACCAGACGTTTGCCCGGATGTTGTACCAGTAGCTGTTGCAGCAACGGGTac > 1:2323829/1‑67 (MQ=255) tGATAAACCCGGTCACCACCAGACGTTTGCCCGGATGTTGTACCAGTAGCTGTTGCAGCAACGGGTac > 1:773682/1‑67 (MQ=255) tGATAAACCCGGTCACCACCAGACGTTTGCCCGGATGTTGTACCAGTAGCTGTTGCAGCAACGGGTac > 1:1147370/1‑67 (MQ=255) tGATAAACCCGGTCACCACCAGACGTTTGCCCGGATGTTGTACCAGTAGCTGTTGCAGCAACGGGTac > 1:1665312/1‑67 (MQ=255) tGATAAACCCGGTCACCACCAGACGTTTGCCCGGATGTTGTACCAGTAGCTGTTGCAGCAACGGGTac > 1:348318/1‑67 (MQ=255) tGATAAACCCGGTCACCACCAGACGTTTGCCCGGATGTTGTACCAGTAGCTGTTGCAGCAACGGGTac > 1:1912541/1‑67 (MQ=255) tGATAAACCCGGTCACCACCAGACGTTTGCCCGGATGTTGTACCAGTAGCTGTTGCAGCAACGGGTac > 1:2986602/1‑67 (MQ=255) | GGAATAGTCGGAACCGTTACGCCCCAGCAGCACCGTTTCACCGGCGTTGTTGCGGCTGATAAATCCGGTCACCACCAGACGTTTGCCCGGATGTTGCACCAGCAGCTGTTGCAGCAACGGGTAA > minE/2301432‑2301555 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |