| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,301,534 | C→T | 100% | L179L (CTG→CTA) | metL ← | fused aspartokinase II and homoserine dehydrogenase II |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,301,534 | 0 | C | T | 93.1% | 24.1 / ‑4.8 | 14 | L179L (CTG→CTA) | metL | fused aspartokinase II and homoserine dehydrogenase II |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (8/5); total (9/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GTTTCACCGGCGTTGTTGCGGCTGATAAATCCGGTCACCACCAGACGTTTGCCCGGATGTTGCACCAGCAGCTGTTGCAGCAACGGGTAAGAAAGCCCTTCATCAACCTGCGGTTG > minE/2301466‑2301581 | gTTTCACCGGCGTTGTTGCGGCTGATAAATCCGGTCACCACCAGACGTTTGCCCGGATGTTGCACcagc > 1:2764215/1‑69 (MQ=255) tCACCGGCGTTGTTGCGGCTGATAAATCCGGTCACCACCAGACGTTTGCCCGGATGTTGCACCagcagc > 1:890958/1‑69 (MQ=255) tCACCGGCGTTGTTGCGGCTGATAAATCCGGTCACCACCAGACGTTTGCCCGGATGTTGCACCagcagc > 1:849182/1‑69 (MQ=255) tCACCGGCGTTGTTGCGGCTGATAAATCCGGTCACCACCAGACGTTTGCCCGGATGTTGCACCagcagc > 1:1701493/1‑69 (MQ=255) tCACCGGCGTTGTTGCGGCTGATAAATCCGGTCACCACCAGACGTTTGCCCGGATGTTGCACCagcagc > 1:1749173/1‑69 (MQ=255) tCACCGGCGTTGTTGCGGCTGATAAATCCGGTCACCACCAGACGTTTGCCCGGATGTTGCACCagcagc > 1:66095/1‑69 (MQ=255) tCACCGGCGTTGTTGCGGCTGATAAATCCGGTCACCACCAGACGTTTGCCCGGATGTTGCACCagcagc > 1:1866413/1‑69 (MQ=255) tCACCGGCGTTGTTGCGGCTGATAAATCCGGTCACCACCAGACGTTTGCCCGGATGTTGCACCagcagc > 1:1926906/1‑69 (MQ=255) tCACCGGCGTTGTTGCGGCTGATAAATCCGGTCACCACCAGACGTTTGCCCGGATGTTGCACCagcagc > 1:2623304/1‑69 (MQ=255) tCACCGGCGTTGTTGCGGCTGATAAATCCGGTCACCACCAGACGTTT‑CCCGGATGTTGCACCAGCAGCt > 1:183073/1‑69 (MQ=255) tGATAAACCCGGTCACCACCAGACGTTTGCCCGGATGTTGTACCAGTAGCTGTTGCAGCAACGGGTac > 1:1147370/1‑67 (MQ=255) tGATAAACCCGGTCACCACCAGACGTTTGCCCGGATGTTGTACCAGTAGCTGTTGCAGCAACGGGTac > 1:773682/1‑67 (MQ=255) tGATAAACCCGGTCACCACCAGACGTTTGCCCGGATGTTGTACCAGTAGCTGTTGCAGCAACGGGTac > 1:348318/1‑67 (MQ=255) tGATAAACCCGGTCACCACCAGACGTTTGCCCGGATGTTGTACCAGTAGCTGTTGCAGCAACGGGTac > 1:2986602/1‑67 (MQ=255) tGATAAACCCGGTCACCACCAGACGTTTGCCCGGATGTTGTACCAGTAGCTGTTGCAGCAACGGGTac > 1:2323829/1‑67 (MQ=255) tGATAAACCCGGTCACCACCAGACGTTTGCCCGGATGTTGTACCAGTAGCTGTTGCAGCAACGGGTac > 1:1912541/1‑67 (MQ=255) tGATAAACCCGGTCACCACCAGACGTTTGCCCGGATGTTGTACCAGTAGCTGTTGCAGCAACGGGTac > 1:1665312/1‑67 (MQ=255) tGATAAACCCGGTCACCACCAGACGTTTGCCCGGATGTTGTACCAGTAGCTGTTGCAGCAACGGGTac > 1:1089442/1‑67 (MQ=255) tttGCCCGGATGTTGTACCAGTAGCTGTTGCAGCAACGGGTACGAAAGCCCTTCATCAACCTGCGGTtg < 1:2574661/69‑1 (MQ=255) tttGCCCGGATGTTGTACCAGTAGCTGTTGCAGCAACGGGTACGAAAGCCCTTCATCAACCTGCGGTtg < 1:1935744/69‑1 (MQ=255) tttGCCCGGATGTTGTACCAGTAGCTGTTGCAGCAACGGGTACGAAAGCCCTTCATCAACCTGCGGTtg < 1:1838756/69‑1 (MQ=255) tttGCCCGGATGTTGTACCAGTAGCTGTTGCAGCAACGGGTACGAAAGCCCTTCATCAACCTGCGGTtg < 1:1240148/69‑1 (MQ=255) tttGCCCGGATGTTGTACCAGTAGCTGTTGCAACAACGGGTACGAAAGCCCTTCATCAACCTGCGGTtg < 1:324370/69‑1 (MQ=255) | GTTTCACCGGCGTTGTTGCGGCTGATAAATCCGGTCACCACCAGACGTTTGCCCGGATGTTGCACCAGCAGCTGTTGCAGCAACGGGTAAGAAAGCCCTTCATCAACCTGCGGTTG > minE/2301466‑2301581 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |