| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,302,032 | C→T | 53.8% | Q13Q (CAG→CAA) | metL ← | fused aspartokinase II and homoserine dehydrogenase II |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,302,032 | 0 | C | T | 53.8% | 10.2 / 89.0 | 104 | Q13Q (CAG→CAA) | metL | fused aspartokinase II and homoserine dehydrogenase II |
| Reads supporting (aligned to +/- strand): ref base C (23/25); new base T (28/28); total (51/53) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.47e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01 | |||||||||||
Only 100 of 109 total aligned reads displayed.
ATGCCCGCGACACGCAAATAACACTTCACATCAGCCAGACTACTGCCACCAAATTTATGCAGCTGACGACCTTTCGCCCCTGCCTGCGCAATCACACTCATTTTTACCCCTTGTTTGCAGCCCGGAAGCCA > minE/2301970‑2302100 | aTGCCCGCGACACGCAAATAACACTTCACATCAGCCAGACTACTGCCACCAAATTTATGCAGCTgacga > 1:830753/1‑69 (MQ=255)aTGCCCGCGACACGCAAATAACACTTCACATCAGCCAGACTACTGCCACCAAATTTATGCAGCTgacga > 1:78836/1‑69 (MQ=255) gCCCGCGACACGCAAATAACACTTCACATCAGCCAGACTACTGCCACCAAATTTATGCAGCTgacgac < 1:2826057/68‑1 (MQ=255) gCCCGCGACACGCAAATAACACTTCACATCAGCCAGACTACTGCCACCAAATTTATGCAGCTgacgac < 1:2116342/68‑1 (MQ=255) gCCCGCGACACGCAAATAACACTTCACATCAGCCAGACTACTGCCACCAAATTTATGCAGCTgacgac < 1:2122621/68‑1 (MQ=255) gCCCGCGACACGCAAATAACACTTCACATCAGCCAGACTACTGCCACCAAATTTATGCAGCTgacgac < 1:538909/68‑1 (MQ=255) gCCCGCGACACGCAAATAACACTTCACATCAGCCAGACTACTGCCACCAAATTTATGCAGCTgacgac < 1:1688715/68‑1 (MQ=255) gCCCGCGACACGCAAATAACACTTCACATCAGCCAGACTACTGCCACCAAATTTATGCAGCTgacgac < 1:560447/68‑1 (MQ=255) gCCCGCGACACGCAAATAACACTTCACATCAGCCAGACTACTGCCACCAAATTTATGCAGCTgacgac < 1:1246425/68‑1 (MQ=255) gCCCGCGACACGCAAATAACACTTCACATCAGCCAGACTACTGCCACCAAATTTATGCAGCTgacgac < 1:3251414/68‑1 (MQ=255) gCCCGCGACACGCAAATAACACTTCACATCAGCCAGACTACTGCCACCAAATTTATGCAGCTgacgac < 1:1772975/68‑1 (MQ=255) gCCCGCGACACGCAAATAACACTTCACATCAGCCAGACTACTGCCACCAAATTTATGCAGCTgacgac < 1:1194654/68‑1 (MQ=255) gCCCGCGACACGCAAATAACACTTCACATCAGCCAGACTACTGCCACCAAATTTATGCAGCTgacgac < 1:836276/68‑1 (MQ=255) gCCCGCGACACGCAAATAACACTTCACATCAGCCAGACTACTGCCACCAAATTTATGCAGCTgacgac < 1:2750428/68‑1 (MQ=255) gCCCGCGACACGCAAATAACACTTCACATCAGCCAGACTACTGCCACCAAATTTATGCAGCTgacgac < 1:1057031/68‑1 (MQ=255) cgACACGCAAATAACACTTCACATCAGCCAGACTACTGCCACCAAATTTATGCAGCTgacgac < 1:2500091/63‑1 (MQ=255) gACACGCAAATAACACTTCACATCAGCCAGACTACTGCCACCAAATTTATGCAGCTGACGACCTTTCGc < 1:917807/69‑1 (MQ=255) gACACGCAAATAACACTTCACATCAGCCAGACTACTGCCACCAAATTTATGCAGCTGACGACCTTTCGc < 1:1682597/69‑1 (MQ=255) gACACGCAAATAACACTTCACATCAGCCAGACTACTGCCACCAAATTTATGCAGCTGACGACCTTTCGc < 1:1209047/69‑1 (MQ=255) gACACGCAAATAACACTTCACATCAGCCAGACTACTGCCACCAAATTTATGCAGCTGACGACCTTTCGc < 1:854667/69‑1 (MQ=255) gCAAATAACACTTCACATCAGCCAGACTACTGCCACCAAATTTATGCAGTTgacga < 1:1010394/56‑1 (MQ=255) gCAAATAACACTTCACATCAGCCAGACTACTGCCACCAAATTTATGCAGTTgacga < 1:2662673/56‑1 (MQ=255) gCAAATAACACTTCACATCAGCCAGACTACTGCCACCAAATTTATGCAGTTgacga < 1:2546229/56‑1 (MQ=255) gCAAATAACACTTCACATCAGCCAGACTACTGCCACCAAATTTATGCAGTTgacga < 1:2465791/56‑1 (MQ=255) gCAAATAACACTTCACATCAGCCAGACTACTGCCACCAAATTTATGCAGTTgacga < 1:263020/56‑1 (MQ=255) gCAAATAACACTTCACATCAGCCAGACTACTGCCACCAAATTTATGCAGTTgacga < 1:2398157/56‑1 (MQ=255) gCAAATAACACTTCACATCAGCCAGACTACTGCCACCAAATTTATGCAGTTgacga < 1:2923441/56‑1 (MQ=255) gCAAATAACACTTCACATCAGCCAGACTACTGCCACCAAATTTATGCAGTTgacga < 1:2932117/56‑1 (MQ=255) gCAAATAACACTTCACATCAGCCAGACTACTGCCACCAAATTTATGCAGTTgacga < 1:3038883/56‑1 (MQ=255) gCAAATAACACTTCACATCAGCCAGACTACTGCCACCAAATTTATGCAGTTgacga < 1:309965/56‑1 (MQ=255) gCAAATAACACTTCACATCAGCCAGACTACTGCCACCAAATTTATGCAGTTgacga < 1:3159320/56‑1 (MQ=255) gCAAATAACACTTCACATCAGCCAGACTACTGCCACCAAATTTATGCAGTTgacga < 1:3316324/56‑1 (MQ=255) gCAAATAACACTTCACATCAGCCAGACTACTGCCACCAAATTTATGCAGTTgacga < 1:3326593/56‑1 (MQ=255) gCAAATAACACTTCACATCAGCCAGACTACTGCCACCAAATTTATGCAGTTgacga < 1:389449/56‑1 (MQ=255) gCAAATAACACTTCACATCAGCCAGACTACTGCCACCAAATTTATGCAGTTgacga < 1:441149/56‑1 (MQ=255) gCAAATAACACTTCACATCAGCCAGACTACTGCCACCAAATTTATGCAGTTgacga < 1:464198/56‑1 (MQ=255) gCAAATAACACTTCACATCAGCCAGACTACTGCCACCAAATTTATGCAGTTgacga < 1:499931/56‑1 (MQ=255) gCAAATAACACTTCACATCAGCCAGACTACTGCCACCAAATTTATGCAGTTgacga < 1:545389/56‑1 (MQ=255) gCAAATAACACTTCACATCAGCCAGACTACTGCCACCAAATTTATGCAGTTgacga < 1:787880/56‑1 (MQ=255) gCAAATAACACTTCACATCAGCCAGACTACTGCCACCAAATTTATGCAGTTgacga < 1:1903679/56‑1 (MQ=255) gCAAATAACACTTCACATCAGCCAGACTACTGCCACCAAATTTATGCAGTTgacga < 1:156347/56‑1 (MQ=255) gCAAATAACACTTCACATCAGCCAGACTACTGCCACCAAATTTATGCAGTTgacga < 1:1364775/56‑1 (MQ=255) gCAAATAACACTTCACATCAGCCAGACTACTGCCACCAAATTTATGCAGTTgacga < 1:1259487/56‑1 (MQ=255) gCAAATAACACTTCACATCAGCCAGACTACTGCCACCAAATTTATGCAGTTgacga < 1:1171445/56‑1 (MQ=255) gCAAATAACACTTCACATCAGCCAGACTACTGCCACCAAATTTATGCAGTTgacga < 1:1781030/56‑1 (MQ=255) gCAAATAACACTTCACATCAGCCAGACTACTGCCACCAAATTTATGCAGTTgacga < 1:2042386/56‑1 (MQ=255) cAAATAACACTTCACATCAGCCAGACTACTGCCACCAAATTTATGCAGTTgacga < 1:2304630/55‑1 (MQ=255) acTTCACATCAGCCAGACTACTGCCACCAAATTTATGCAGCTGACGAcc > 1:1124117/1‑49 (MQ=255) acTTCACATCAGCCAGACTACTGCCACCAAATTTATGCAGCTGACGAcc > 1:623753/1‑49 (MQ=255) acTTCACATCAGCCAGACTACTGCCACCAAATTTATGCAGCTGACGAcc > 1:607880/1‑49 (MQ=255) acTTCACATCAGCCAGACTACTGCCACCAAATTTATGCAGCTGACGAcc > 1:2227844/1‑49 (MQ=255) acTTCACATCAGCCAGACTACTGCCACCAAATTTATGCAGCTGACGAcc > 1:3298203/1‑49 (MQ=255) acTTCACATCAGCCAGACTACTGCCACCAAATTTATGCAGCTGACGAcc > 1:2243511/1‑49 (MQ=255) cAGCCAGACTACTGCCACCAAATTTATGCAGCTGACGACCTTTCGCCCCTGCCTGCGCAATCACACTCa < 1:2471729/69‑1 (MQ=255) aGCCAGACTACTGCCACCAAATTTATGCAGCTGACGACCTTTCGCCcct > 1:1193380/1‑49 (MQ=255) aGCCAGACTACTGCCACCAAATTTATGCAGCTGACGACCTTTCGCCCCTGCCTGCGCAATCACACTCa > 1:1621388/1‑68 (MQ=255) aGCCAGACTACTGCCACCAAATTTATGCAGCTGACGACCTTTCGCCCCTGCCTGCGCAATCACACTCAt < 1:2892065/69‑1 (MQ=255) aGCCAGACTACTGCCACCAAATTTATGCAGCTGACGACCTTTCGCCCCTGCCTGCGCAATCACACTCAt > 1:2926467/1‑69 (MQ=255) aGCCAGACTACTGCCACCAAATTTATGCAGCTGACGACCTTTCGCCCCTGCCTGCGCAATCACACTCAt < 1:2028112/69‑1 (MQ=255) aGCCAGACTACTGCCACCAAATTTATGCAGCTGACGACCTTTCGCCCCTGCCTGCGCAATCACACTCAt < 1:2143334/69‑1 (MQ=255) aGCCAGACTACTGCCACCAAATTTATGCAGCTGACGACCTTTCGCCCCTGCCTGCGCAATCACACTCAt < 1:107564/69‑1 (MQ=255) gCCAGACTACTGCCACCAAATTTATGCAGTTgacga < 1:1470695/36‑1 (MQ=255) ctactGCCACCAAATTTATGCAGCTGACGACCTTTCGCCCCTGCCTGCGCAATCacac > 1:2693060/1‑58 (MQ=255) ctactGCCACCAAATTTATGCAGCTGACGACCTTTCGCCCCTGCCTGCGCAATCACACTCATTTTTAcc > 1:1991537/1‑69 (MQ=255) ctactGCCACCAAATTTATGCAGCTGACGACCTTTCGCCCCTGCCTGCGCAATCACACTCATTTTTAcc > 1:2893133/1‑69 (MQ=255) ctactGCCACCAAATTTATGCAGCTGACGACCTTTCGCCCCTGCCTGCGCAATCACACTCATTTTTAcc > 1:702137/1‑69 (MQ=255) ctactGCCACCAAATTTATGCAGCTGACGACCTTTCGCCCCTGCCTGCGCAATCACACTCATTTTTAcc > 1:1899973/1‑69 (MQ=255) ctactGCCACCAAATTTATGCAGCTGACGACCTTTCGCCCCTGCCTGCGCAATCACACTCATTTTTAcc > 1:2540280/1‑69 (MQ=255) ctactGCCACCAAATTTATGCAGCTGACGACCTTTCGCCCCTGCCTGCGCAATCACACTCATTTTTAcc > 1:1451225/1‑69 (MQ=255) ccaccaAATTTATGCAGTTGACGACCTTTCGCCCCTGCCTGCGCAATCACACTCATTTTTACCCCTTGt > 1:1715056/1‑69 (MQ=255) ccaccaAATTTATGCAGTTGACGACCTTTCGCCCCTGCCTGCGCAATCACACTCATTTTTACCCCTTGt > 1:625479/1‑69 (MQ=255) ccaccaAATTTATGCAGTTGACGACCTTTCGCCCCTGCCTGCGCAATCACACTCATTTTTACCCCTTGt > 1:897566/1‑69 (MQ=255) ccaccaAATTTATGCAGTTGACGACCTTTCGCCCCTGCCTGCGCAATCACACTCATTTTTACCCCTTGt > 1:59915/1‑69 (MQ=255) ccaccaAATTTATGCAGTTGACGACCTTTCGCCCCTGCCTGCGCAATCACACTCATTTTTACCCCTTGt > 1:1784663/1‑69 (MQ=255) ccaccaAATTTATGCAGTTGACGACCTTTCGCCCCTGCCTGCGCAATCACACTCATTTTTACCCCTTGt > 1:3272346/1‑69 (MQ=255) ccaccaAATTTATGCAGTTGACGACCTTTCGCCCCTGCCTGCGCAATCACACTCATTTTTACCCCCTGt > 1:762773/1‑69 (MQ=255) accaAATTTATGCGGTTGACGACCTTTCGCCCCTGCCTGCGCAATCACACTCATTTTTACCCCTTGtt > 1:2239565/1‑68 (MQ=255) accaAATTTATGCAGTTGACGTCCTTTCGCCCCTGCCTGCGCAATCACACTCATTTTTACCCCTTGtt > 1:2811132/1‑68 (MQ=255) accaAATTTATGCAGTTGACGCCCTTTCGCCCCTGCCTGCGCAATCACACTCATTTTTACCCCTTGtt > 1:578597/1‑68 (MQ=255) accaAATTTATGCAGTTGACGACCTTTCGCCCCTGCCTGCGCAATCACACTCATTTTTAccc > 1:2289382/1‑62 (MQ=255) accaAATTTATGCAGTTGACGACCTTTCGCCCCTGCCTGCGCAATCACACTCATTTTTACCCCTTGtt > 1:615947/1‑68 (MQ=255) accaAATTTATGCAGTTGACGACCTTTCGCCCCTGCCTGCGCAATCACACTCATTTTTACCCCTTGtt > 1:1049964/1‑68 (MQ=255) accaAATTTATGCAGTTGACGACCTTTCGCCCCTGCCTGCGCAATCACACTCATTTTTACCCCTTGtt > 1:2401573/1‑68 (MQ=255) accaAATTTATGCAGTTGACGACCTTTCGCCCCTGCCTGCGCAATCACACTCATTTTTACCCCTTGtt > 1:2031477/1‑68 (MQ=255) accaAATTTATGCAGTTGACGACCTTTCGCCCCTGCCTGCGCAATCACACTCATTTTTACCCCTTGtt > 1:805686/1‑68 (MQ=255) accaAATTTATGCAGTTGACGACCTTTCGCCCCTGCCTGCGCAATCACACTCATTTTTACCCCTTGtt > 1:2705202/1‑68 (MQ=255) accaAATTTATGCAGTTGACGACCTTTCGCCCCTGCCTGCGCAATCACACTCATTTTTACCCCTTGtt > 1:1221562/1‑68 (MQ=255) accaAATTTATGCAGTTGACGACCTTTCGCCCCTGCCTGCGCAATCACACTCATTTTTACCCCTTGtt > 1:301381/1‑68 (MQ=255) accaAATTTATGCAGTTGACGACCTTTCGCCCCTGCCTGCGCAATCACACTCATTTTTACCCCTTGtt > 1:3066551/1‑68 (MQ=255) accaAATTTATGCAGTTGACGACCTTTCGCCCCTGCCTGCGCAATCACACTCATTTTTACCCCTTGtt > 1:1780429/1‑68 (MQ=255) accaAATTTATGCAGTTGACGACCTTTCGCCCCTGCCTGCGCAATCACACTCATTTTTACCCCTTGtt > 1:513720/1‑68 (MQ=255) accaAATTTATGCAGTTGACGACCTTTCGCCCCTGCCTGCGCAATCACACTCATTTTTACCCCTTGtt > 1:1399635/1‑68 (MQ=255) accaAATTTATGCAGTTGACGACCTTTCGCCCCTGCCTGCGCAATCACACTCATTTTTACCCCTTGtt > 1:1401641/1‑68 (MQ=255) accaAATTTATGCAGTTGACGACCTTTCGCCCCTGCCTGCGCAATCACACTCATTTTTACCCCTTGtt > 1:3221856/1‑68 (MQ=255) accaAATTTATGCAGTTGACGACCTTTCGCCCCTGCCTGCGCAATCACACTCATTTTTACCCCTTGtt > 1:361228/1‑68 (MQ=255) cTGACGACCTTTCGCCCCTGCCTGCGCAATCACACTCATTTTTACCCCTTGTTTGCAGCCCGGAAGCCa < 1:1947773/69‑1 (MQ=255) cTGACGACCTTTCGCCCCTGCCTGCGCAATCACACTCATTTTTACCCCTTGTTTGCAGCCCGGAAGCCa < 1:2712287/69‑1 (MQ=255) cTGACGACCTTTCGCCCCTGCCTGCGCAATCACACTCATTTTTACCCCTTGTTTGCAGCCCGGAAGCCa < 1:1381360/69‑1 (MQ=255) cTGACGACCTTTCGCCCCTGCCTGCGCAATCACACTCATTTTTACCCCTTGTTTGCAGCCCGGAAGCCa < 1:821423/69‑1 (MQ=255) cTGACGACCTTTCGCCCCTGCCTGCGCAATCACACTCATTTTTACCCCTTGTTTGCAGCCCGGAAGCCa < 1:2124144/69‑1 (MQ=255) | ATGCCCGCGACACGCAAATAACACTTCACATCAGCCAGACTACTGCCACCAAATTTATGCAGCTGACGACCTTTCGCCCCTGCCTGCGCAATCACACTCATTTTTACCCCTTGTTTGCAGCCCGGAAGCCA > minE/2301970‑2302100 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |