BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,306,411	T→C	19.9%	C439C (TGT→TGC)	priA →	primosome factor n'

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,306,411	0	T	C	19.9%	152.4 / 27.2	105	C439C (TGT→TGC)	priA	primosome factor n'
Reads supporting (aligned to +/- strand): ref base T (49/35); new base C (12/9); total (61/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.42e-01

Only 100 of 114 total aligned reads displayed.

AACCAGGTCATTCTCTTTCTTAACCGCCGTGGCTTTGCGCCTGCACTGCTGTGCCACGACTGTGGCTGGATTGCCGAATGCCCACGTTGCGATCACTACTACACGCTGCATCAGGCGCAGCACCATCTGC  >  minE/2306349‑2306478

aaCCAGGTCATTCTCTTTCTTAACCGCCGTGGCTTTGCGCCTGCACTGCTGTGCCACGACTGTGGCTgg                                                               <  1:9469/69‑1 (MQ=255)

aaCCAGGTCATTCTCTTTCTTAACCGCCGTGGCTTTGCGCCTGCACTGCTGTGCCACGACTGTGGCTgg                                                               <  1:463112/69‑1 (MQ=255)

aaCCAGGTCATTCTCTTTCTTAACCGCCGTGGCTTTGCGCCTGCACTGCTGTGCCACGACTGTGGCTgg                                                               <  1:2961666/69‑1 (MQ=255)

aaCCAGGTCATTCTCTTTCTTAACCGCCGTGGCTTTGCGCCTGCACTGCTGTGCCACGACTGTGGCTgg                                                               <  1:2434545/69‑1 (MQ=255)

    aGGTTATCCTCTTTCTTAATCGTCGGGGTTTTGCGCCTGCGCTGCTGTGCCACGACTGCGGCTGGAtag                                                           <  1:1026581/69‑3 (MQ=255)

    aGGTTATCCTCTTTCTTAATCGTCGGGGTTTTGCGCCTGCGCTGCTGTGCCACGACTGCGGCTGGAtag                                                           <  1:2635515/69‑3 (MQ=255)

    aGGTTATCCTCTTTCTTAATCGTCGGGGTTTTGCGCCTGCGCTGCTGTGCCACGACTGCGGCTGGAtag                                                           <  1:2090985/69‑3 (MQ=255)

    aGGTTATCCTCTTTCTTAATCGTCGGGGTTTTGCGCCTGCGCTGCTGTGCCACGACTGCGGCTGGAtag                                                           <  1:1770784/69‑3 (MQ=255)

    aGGTTATCCTCTTTCTTAATCGTCGGGGTTTTGCGCCTGCGCTGCTGTGCCACGACTGCGGCTGGAtag                                                           <  1:1766827/69‑3 (MQ=255)

    aGGTTATCCTCTTTCTTAATCGTCGGGGTTTTGCGCCTGCGCTGCTGTGCCACGACTGCGGCTGGAtag                                                           <  1:1431832/69‑3 (MQ=255)

      gTCATTCTCTTTCTTAACCGCCGTGGCTTTGCGCCTGCACTTCTGTGCCACGACTGTGGc                                                                  >  1:3042013/1‑60 (MQ=255)

      gTCATTCTCTTTCTTAACCGCCGTGGCTTTGCGCCTGCACTGCTGTGCCACGACTGTGGCTGGATTGc                                                          >  1:1885817/1‑68 (MQ=255)

      gTCATTCTCTTTCTTAACCGCCGTGGCTTTGCGCCTGCACTGCTGTGCCACGACTGTGGCTGGATTGc                                                          >  1:1245443/1‑68 (MQ=255)

      gTCATTCTCTTTCTTAACCGCCGTGGCTTTGCGCCTGCACTGCTGTGCCACGACTGTGGCTGGATTGc                                                          >  1:466214/1‑68 (MQ=255)

        cATTCTCTTTCTTAACCGCCGTGGCTTTGCGCCTGCACTGCTGTGCCACGACTGTGGCTGGATTGCCGa                                                       >  1:3241589/1‑69 (MQ=255)

        cATTCTCTTTCTTAACCGCCGTGGCTTTGCGCCTGCACTGCTGTGCCACGACTGTGGCTGGATTGCCGa                                                       >  1:1975479/1‑69 (MQ=255)

         aTTCTCTTTCTTAACCGCCGTGGCTTTGCGCCTGCACTGCTGTGCCACGACTGTGGCTGGATTGCCGaa                                                      >  1:29487/1‑69 (MQ=255)

         aTTCTCTTTCTTAACCGCCGTGGCTTTGCGCCTGCACTGCTGTGCCACGACTGTGGCTGGATTGCCGaa                                                      >  1:3165598/1‑69 (MQ=255)

         aTTCTCTTTCTTAACCGCCGTGGCTTTGCGCCTGCACTGCTGTGCCACGACTGTGGCTGGATTGCCGaa                                                      <  1:3045361/69‑1 (MQ=255)

         aTTCTCTTTCTTAACCGCCGTGGCTTTGCGCCTGCACTGCTGTGCCACGACTGTGGCTGGATTGCCGaa                                                      >  1:1763186/1‑69 (MQ=255)

         aTTCTCTTTCTTAACCGCCGTGGCTTTGCGCCTGCACTGCTGTGCCACGACTGTGGCTGGATTGCCGaa                                                      >  1:821783/1‑69 (MQ=255)

         aTTCTCTTTCTTAACCGCCGTGGCTTTGCGCCTGCACTGCTGTGCCAAGACTGTGGCTGGATTGCCGaa                                                      >  1:4830/1‑69 (MQ=255)

                  cttAACCGCCGTGGCTTTGCGCCTGCACTGCTGTGCCACGACTGTGGCTGGATTGCCGAATGCCCACGt                                             >  1:2905135/1‑69 (MQ=255)

                  cttAACCGCCGTGGCTTTGCGCCTGCACTGCTGTGCCACGACTGTGGCTGGATTGCCGAATGCCCACGt                                             >  1:1269276/1‑69 (MQ=255)

                  cttAACCGCCGTGGCTTTGCGCCTGCACTGCTGTGCCACGACTGTGGCTGGATTGCCGAATGCCCACGt                                             >  1:868298/1‑69 (MQ=255)

                  cttAACCGCCGTGGCTTTGCGCCTGCACTGCTGTGCCACGACTGTGGCTGGATTGCCGAATGCCCACGt                                             >  1:1875000/1‑69 (MQ=255)

                     aaCCGCCGTGGCTTTGCGCCTGCACTGCTGTGCCCCGACTGTGGCTGGATTGCCGAATGCCCACGTTGc                                          >  1:85101/1‑69 (MQ=255)

                     aaCCGCCGTGGCTTTGCGCCTGCACTGCTGTGCCACGACTGTGGCTGGATTGCCGAATGCCCACGTTGc                                          >  1:2592297/1‑69 (MQ=255)

                     aaCCGCCGTGGCTTTGCGCCTGCACTGCTGTGCCACGACTGTGGCTGGATTGCCGAATGCCCACGTTGc                                          >  1:3007460/1‑69 (MQ=255)

                     aaCCGCCGTGGCTTTGCGCCTGCACTGCTGTGCCACGACTGTGGCTGGATTGCCGAATGCCCACGTTGc                                          >  1:2685901/1‑69 (MQ=255)

                     aaCCGCCGTGGCTTTGCGCCTGCACTGCTGTGCCACGACTGTGGCTGGATTGCCGAATGCCCACGTTGc                                          >  1:1212747/1‑69 (MQ=255)

                     aaCCGCCGTGGCTTTGCGCCTGCACTGCTGTGCCACGACTGTGGCTGGATTGCCGAATGCCCACGTTGc                                          >  1:247348/1‑69 (MQ=255)

                     aaCCGCCGTGGCTTTGCGCCTGCACTGCTGTGCCACGACTGTGGCTGGATTGCCGAATGCCCACGTTGc                                          >  1:1236391/1‑69 (MQ=255)

                     aaCCGCCGTGGCTTTGCGCCTGCACTGCTGTGCCACGACTGTGGCTGGATTGCCGAATGCCCACGTTGc                                          >  1:1682820/1‑69 (MQ=255)

                     aaCCGCCGTGGCTTTGCGCCTGCACTGCTGTGCCACGACTGTGGCTGGATTGCCGAATGCCCACGTTGc                                          >  1:1366758/1‑69 (MQ=255)

                     aaCCGCCGTGGCTTTGCGCCTGCACTGCTGTGCCACGACTGTGGCTGGATTGCCGAATGCCCACGTTGc                                          >  1:1387824/1‑69 (MQ=255)

                     aaCCGCCGTGGCTTTGCGCCTGCACTGCTGTGCCACGACTGTGGCTGGATTGCCGAATGACCACGTTGc                                          >  1:1938893/1‑69 (MQ=255)

                       ccgccgTGGCTTTGCGCCTGCACTGCTGTGCCACGACTGTGGCTGGATTGCCGAATGCCCACGTTGCg                                         <  1:1970516/68‑1 (MQ=255)

                       ccgccgTGGCTTTGCGCCTGCACTGCTGTGCCACGACTGTGGCTGGATTGCCGAATGCCCACGTTGCg                                         <  1:1971261/68‑1 (MQ=255)

                       ccgccgTGGCTTTGCGCCTGCACTGCTGTGCCACGACTGTGGCTGGATTGCCGAATGCCCACGTTGCg                                         <  1:383176/68‑1 (MQ=255)

                       ccgccgTGGCTTTGCGCCTGCACTGCTGTGCCACGACTGTGGCTGGATTGCCGAATGCCCACGTTGCg                                         <  1:1612159/68‑1 (MQ=255)

                       ccgccgTGGCTTTGCGCCTGCACTGCTGTGCCACGACTGTGGCTGGATTGCCGAATGCCCACGTTGCg                                         <  1:2691566/68‑1 (MQ=255)

                       ccgccgTGGCTTTGCGCCTGCACTGCTGTGCCACGACTGTGGCTGGATTGCCGAATGCCCACGTTGCg                                         <  1:2449730/68‑1 (MQ=255)

                        cgccgTGGCTTTGCGCCTGCACTGCTGTGCCACGACTGTGGCTGGATTGCCGAATGCCCACGTTGCg                                         <  1:373259/67‑1 (MQ=255)

                         gccgTGGCTTTGCGCCTGCACTGCTGTGCCACGACTGTGGCTGGATTGCCGAATGCCCACGTTGCg                                         <  1:1214614/66‑1 (MQ=255)

                                 tttGCGCCTGCACTGCTGTGCCACGACTGTGGCTGGATTGCCGAATGCCCACGTTGCGATCActacta                               <  1:2248867/68‑1 (MQ=255)

                                 tttGCGCCTGCACTGCTGTGCCACGACTGTGGCTGGATTGCCGAATGCCCACGTTGCGATCActacta                               <  1:2616504/68‑1 (MQ=255)

                                 tttGCGCCTGCACTGCTGTGCCACGACTGTGGCTGGATTGCCGAATGCCCACGTTGCGATCActacta                               <  1:864286/68‑1 (MQ=255)

                                 tttGCGCCTGCACTGCTGTGCCACGACTGTGGCTGGATTGCCGAATGCCCACGTTGCGATCActacta                               <  1:1765973/68‑1 (MQ=255)

                                        cTGCACTGCTGTGCCACGACTGTGGCTGGATTGCCGAATGCCCACGTTGCGATCACTACTACACGCTg                        >  1:1020126/1‑68 (MQ=255)

                                        cTGCACTGCTGTGCCACGACTGTGGCTGGATTGCCGAATGCCCACGTTGCGATCACTACTACACGCTGc                       >  1:1810806/1‑69 (MQ=255)

                                        cTGCACTGCTGTGCCACGACTGTGGCTGGATTGCCGAATGCCCACGTTGCGATCACTACTACACGCTGc                       >  1:814305/1‑69 (MQ=255)

                                         tGCACTGCTGTGCCACGACTGTGGCTGGATTGCCGAATGCCCACGTTGCGATCActacta                               <  1:446649/60‑1 (MQ=255)

                                               gctgTGCCACGACTGTGGCTGGATTGCCGAATGCCCACTTTGCg                                         <  1:1064400/44‑1 (MQ=255)

                                               gctgTGCCACGACTGTGGCTGGATTGCCGAATGCCCACGTTGCg                                         <  1:1346582/44‑1 (MQ=255)

                                               gctgTGCCACGACTGTGGCTGGATTGCCGAATGCCCACGTTGCg                                         <  1:379437/44‑1 (MQ=255)

                                               gctgTGCCACGACTGTGGCTGGATTGCCGAATGCCCACGTTGCg                                         <  1:565760/44‑1 (MQ=255)

                                               gctgTGCCACGACTGTGGCTGGATTGCCGAATGCCCACGTTGCg                                         <  1:2372845/44‑1 (MQ=255)

                                               gctgTGCCACGACTGTGGCTGGATTGCCGAATGCCCACGTTGCg                                         <  1:2736752/44‑1 (MQ=255)

                                               gctgTGCCACGACTGTGGCTGGATTGCCGAATGCCCACGTTGCg                                         <  1:2530200/44‑1 (MQ=255)

                                               gctgTGCCACGACTGTGGCTGGATTGCCGAATGCCCACGTTGCg                                         <  1:3365085/44‑1 (MQ=255)

                                               gctgTGCCACGACTGTGGCTGGATTGCCGAATGCCCACGTTGCg                                         <  1:1071143/44‑1 (MQ=255)

                                               gctgTGCCACGACTGTGGCTGGATTGCCGAATGCCCACGTTGCg                                         <  1:3191177/44‑1 (MQ=255)

                                               gctgTGCCACGACTGTGGCTGGATTGCCGAATGCCCACGTTGCg                                         <  1:2044870/44‑1 (MQ=255)

                                               gctgTGCCACGACTGTGGCTGGATTGCCGAATGCCCACGTTGCGATCActacta                               <  1:1637796/54‑1 (MQ=255)

                                               gctgTGCCACGACTGTGGCTGGATTGCCGAATGCCCACGTTGCGATCACTACTACACGCTGCATCAgg                 >  1:2098378/1‑68 (MQ=255)

                                               gctgTGCCACGACTGTGGCTGGATTGCCGAATGCCCACGTTGCGATCACTACTACACGCTGCATCAgg                 >  1:1653462/1‑68 (MQ=255)

                                               gctgTGCCACGACTGTGGCTGGATTGCCGAATGCCCACGTTGCGATCACTACTACACGCTGCATCAgg                 >  1:2686750/1‑68 (MQ=255)

                                               gctgTGCCACGACTGTGGCTGGATTGCCGAATGCCCACGTTGCGATCACTACTACACGCTGCATCAgg                 >  1:2899260/1‑68 (MQ=255)

                                               gctgTGCCACGACTGTGGCTGGATTGCCGAATGCCCACGTTGCGATCACTACTACACGCTGCATCAgg                 >  1:2161758/1‑68 (MQ=255)

                                               gctgTGCCACGACTGTGGCTGGATTGCCGAATGCCCACGTTGCGATCACTACTACACGCTGCATCAgg                 >  1:2138303/1‑68 (MQ=255)

                                               gctgTGCCACGACTGTGGCTGGATTGCCGAATGCCCACGTTGCGATCACTACTACACGCTGCATCAgg                 >  1:3175454/1‑68 (MQ=255)

                                               gctgTGCCACGACTGTGGCTGGATTGCCGAATGCCCACGTTGCGATCACTACTACACGCTGCATCAgg                 >  1:3156866/1‑68 (MQ=255)

                                               gctgTGCCACGACTGTGGCTGGATTGCCGAATGCCCACGTAGCGATCACTACTacac                            >  1:3124323/1‑57 (MQ=255)

                                                       aCGACTGTGGCTGGATTGCCGAATGCCCACGTTGCGATCACTACTACACGCTGCATCAGGCGCAGCAc         <  1:582889/68‑1 (MQ=255)

                                                          aCTGTGGCTGGATTGCCGAATGCCCACGTTGCGATCACTACTACACGCTGCATCa                   >  1:551977/1‑55 (MQ=255)

                                                          aCTGTGGCTGGATTGCCGAATGCCCACGTTGCGATCACTACTACACGCTGCATCAGGCGCAGCACCATc     >  1:596616/1‑69 (MQ=255)

                                                          aCTGTGGCTGGATTGCCGAATGCCCACGTTGCGATCACTACTACACGCTGCATCAGGCGCAGCACCATc     >  1:415140/1‑69 (MQ=255)

                                                          aCTGTGGCTGGATTGCCGAATGCCCACGTTGCGATCACTACTACACGCTGCATCAGGCGCAGCACCATc     >  1:1462674/1‑69 (MQ=255)

                                                          aCTGTGGCTGGATAGCCGAATGCCCACGTTGCGATCACTACTACACGCTGCATCAGGCGCAGCACCATc     >  1:497873/1‑69 (MQ=255)

                                                           cTGCGGCTGGATAGCCGAATGCCCACGTTGCGATCACTACTACACGCTGCATCAGGCGCAACAGCAttt    >  1:1498031/1‑67 (MQ=255)

                                                           cTGCGGCTGGATAGCCGAATGCCCACGTTGCGATCACTACTACACGCTGCATCAGGCGCAACAGCAttt    >  1:711980/1‑67 (MQ=255)

                                                           cTGCGGCTGGATAGCCGAATGCCCACGTTGCGATCACTACTACACGCTGCATCAGGCGCAACAGCAttt    >  1:855195/1‑67 (MQ=255)

                                                           cTGCGGCTGGATAGCCGAATGCCCACGTTGCGATCACTACTACACGCTGCATCAGGCGCAACAGCAttt    >  1:1417333/1‑67 (MQ=255)

                                                           cTGCGGCTGGATAGCCGAATGCCCACGTTGCGATCACTACTACACGCTGCATCAGGCGCAACAGCAttt    >  1:3435163/1‑67 (MQ=255)

                                                           cTGCGGCTGGATAGCCGAATGCCCACGTTGCGATCACTACTACACGCTGCATCAGGCGCAACAGCAttt    >  1:384355/1‑67 (MQ=255)

                                                           cTGCGGCTGGATAGCCGAATGCCCACGTTGCGATCACTACTACACGCTGCATCAGGCGCAACAGCAttt    >  1:1754224/1‑67 (MQ=255)

                                                           cTGCGGCTGGATAGCCGAATGCCCACGTTGCGATCACTACTACACGCTGCATCAGGCGCAACAGCAttt    >  1:3285868/1‑67 (MQ=255)

                                                           cTGCGGCTGGATAGCCGAATGCCCACGTTGCGATCACTACTACACGCTGCATCAGGCGCAACAGCAttt    >  1:3201592/1‑67 (MQ=255)

                                                           cTGCGGCTGGATAGCCGAATGCCCACGTTGCGATCACTACTACACGCTGCATCAGGCGCAACAGCAttt    >  1:2469004/1‑67 (MQ=255)

                                                           cTGCGGCTGGATAGCCGAATGCCCACGTTGCGATCACTACTACACGCTGCATCAGGCGCAACAGCAttt    >  1:252014/1‑67 (MQ=255)

                                                           cTGCGGCTGGATAGCCGAATGCCCACGTTGCGATCACTACTACACGCTGCATCAGGCGCAACAGCAtt     >  1:1616717/1‑67 (MQ=255)

                                                             gtgGCTGGATTGCCGAATGCCCACGTTGCGATCACTACTACACGCTGCATCAGGCGCAGCACCATCTgc  >  1:1714050/1‑69 (MQ=255)

                                                             gtgGCTGGATTGCCGAATGCCCACGTTGCGATCACTACTACACGCTGCATCAGGCGCAGCACCATCTgc  >  1:553189/1‑69 (MQ=255)

                                                             gtgGCTGGATTGCCGAATGCCCACGTTGCGATCACTACTACACGCTGCATCAGGCGCAGCACCATCTgc  >  1:560288/1‑69 (MQ=255)

                                                             gtgGCTGGATTGCCGAATGCCCACGTTGCGATCACTACTACACGCTGCATCAGGCGCAGCACCATCTgc  >  1:1830786/1‑69 (MQ=255)

                                                             gtgGCTGGATTGCCGAATGCCCACGTTGCGATCACTACTACACGCTGCATCAGGCGCAGCACCATCTgc  >  1:3126025/1‑69 (MQ=255)

                                                             gtgGCTGGATTGCCGAATGCCCACGTTGCGATCACTACTACACGCTGCATCAGGCGCAGCACCATCTgc  >  1:1213216/1‑69 (MQ=255)

                                                             gtgGCTGGATTGCCGAATGCCCACGTTGCGATCACTACTACACGCTGCATCAGGCGCAGCACCATCTgc  >  1:2614449/1‑69 (MQ=255)

                                                             gtgGCCGGATTGCCGAATGCCCACGTTGCGATCACTACTACACGCTGCATCAGGCGCAGCACCATCTgc  >  1:2262700/1‑69 (MQ=255)

AACCAGGTCATTCTCTTTCTTAACCGCCGTGGCTTTGCGCCTGCACTGCTGTGCCACGACTGTGGCTGGATTGCCGAATGCCCACGTTGCGATCACTACTACACGCTGCATCAGGCGCAGCACCATCTGC  >  minE/2306349‑2306478

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`