Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	195,640	A→T	25.0%	P63P (CCT→CCA)	yaeF ←	predicted lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	195,640	0	A	T	25.0%	64.1 / 20.0	44	P63P (CCT→CCA)	yaeF	predicted lipoprotein
Reads supporting (aligned to +/- strand):  ref base A (27/6);  new base T (9/2);  total (36/8)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.73e-01

GATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAACAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTCGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATG  >  minE/195580‑195707                                                             |                                                                    gATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAACAGCAAATCGCCGGGTTTGAGATCAGGTGCCGt                                                             <  1:2015893/69‑1 (MQ=255)   ttCCAAATGAGGTTACCCCAAGGCTTGAGGAGAACAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTa                                                           >  1:896058/1‑69 (MQ=255)   ttCCAAATGAGGTTACCCCAAGGCTTGAGGAGAACAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTa                                                           >  1:445003/1‑69 (MQ=255)   ttCCAAATGAGGTTACCCCAAGGCTTGAGGAGAACAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTa                                                           >  1:3354838/1‑69 (MQ=255)   ttCCAAATGAGGTTACCCCAAGGCTTGAGGAGAACAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTa                                                           >  1:1505962/1‑69 (MQ=255)   ttCCAAATGAGGTTACCCCAAGGCTTGAGGAGAACAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTa                                                           >  1:2444659/1‑69 (MQ=255)        aaTGAGGTTACCCCAAGGCTTGAGGAGAACAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTc                                                       <  1:1837318/68‑1 (MQ=255)              gTTACCCCAAGGCTTGAGGAGAACAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTatt                                                >  1:2011114/1‑69 (MQ=255)                 aCCCCAAGGCTTGAGGAGAACAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTattgat                                             >  1:1203453/1‑69 (MQ=255)                 aCCCCAAGGCTTGAGGAGAACAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTattgat                                             >  1:3389882/1‑69 (MQ=255)                 aCCCCAAGGCTTGAGGAGAACAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTattga                                              >  1:1363973/1‑68 (MQ=255)                 aCCCCAAGGCTTGAGGAGAAAAGCAAATCGCCGGGTTTGAGATCTGGTTCCGTTATTTCTTTTattgat                                             >  1:3135533/1‑69 (MQ=255)                 aCCCCAAGGCTTGAGGAGAAAAGCAAATCGCCGGGTTTGAGATCTGGTTCCGTTATTTCTTTTattgat                                             >  1:846489/1‑69 (MQ=255)                 aCCCCAAGGCTTGAGGAGAAAAGCAAATCGCCGGGTTTGAGATCTGGTTCCGTTATTTCTTTTattgat                                             >  1:3276120/1‑69 (MQ=255)                 aCCCCAAGGCTTGAGGAGAAAAGCAAATCGCCGGGTTTGAGATCTGGTTCCGTTATTTCTTTTattgat                                             >  1:2005231/1‑69 (MQ=255)                 aCCCCAAGGCTTGAGGAGAAAAGCAAATCGCCGGGTTTGAGATCTGGTTCCGTTATTTCTTTTattgat                                             >  1:1558671/1‑69 (MQ=255)                 aCCCCAAGGCTTGAGGAGAAAAGCAAATCGCCGGGTTTGAGATCTGGTTCCGTTATTTCTTTTattgat                                             >  1:1520198/1‑69 (MQ=255)                 aCCCCAAGGCTTGAGGAGAAAAGCAAATCGCCGGGTTTGAGATCTGGTTCCGTTATTTCTTTTattgat                                             >  1:2480217/1‑69 (MQ=255)                 aCCCCAAGGCTTGAGGAGAAAAGCAAATCGCCGGGTTTGAGATCTGGTTCCGTTATTTCTTTTattgat                                             >  1:1238553/1‑69 (MQ=255)                 aCCCCAAGGCTTGAGGAGAAAAGCAAATCGCCGGGTTTGAGATCTGGTTCCGTTATTTCTTTTattgat                                             >  1:2784605/1‑69 (MQ=255)                                  gaACAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTCGGTGAAAGAGCt                            >  1:1739732/1‑69 (MQ=255)                                  gaACAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTCGGTGAAAGAGCt                            >  1:3153741/1‑69 (MQ=255)                                  gaACAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTCGGTGAAAGAGCt                            >  1:150014/1‑69 (MQ=255)                                  gaACAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTCGGTGAAAGAGCt                            >  1:2865340/1‑69 (MQ=255)                                  gaACAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTCGGTGAAAGAGCt                            >  1:2712703/1‑69 (MQ=255)                                  gaACAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTCGGTGAAAGAGCt                            >  1:799008/1‑69 (MQ=255)                                  gaACAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTCGGTGAAAGAGCt                            >  1:1945064/1‑69 (MQ=255)                                    aaaGCAAATCGCCGGGTTTTAGATCTGGTTCCGTTATTTCTTTTATTGATTGTTCGGTGAAAGAGCttt                          <  1:2774392/67‑1 (MQ=255)                                    aaaGCAAATCGCCGGGTTTGAGATCTGGTTCCGTTATTTCTTTTATTGATTGTTCGGTGAAAGAGCttt                          <  1:2710893/67‑1 (MQ=255)                                            tCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTCGGTGAAAGAGCTTTGATGCTgg                  >  1:1582410/1‑69 (MQ=255)                                                      tGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTCGGTGAAAGAGCttt                          >  1:480092/1‑51 (MQ=255)                                                      tGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTCGGTGAAAGAGCttt                          >  1:1075031/1‑51 (MQ=255)                                                      tGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTCGGTGAAAGAGCttt                          >  1:3029544/1‑51 (MQ=255)                                                      tGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTCGGTGAAAGAGCttt                          >  1:2934755/1‑51 (MQ=255)                                                      tGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTCGGTGAAAGAGCttt                          >  1:2933506/1‑51 (MQ=255)                                                      tGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTCGGTGAAAGAGCttt                          >  1:2868706/1‑51 (MQ=255)                                                      tGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTCGGTGAAAGAGCttt                          >  1:285434/1‑51 (MQ=255)                                                      tGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTCGGTGAAAGAGCttt                          >  1:2452255/1‑51 (MQ=255)                                                      tGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTCGGTGAAAGAGCttt                          >  1:1836587/1‑51 (MQ=255)                                                      tGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTCGGTGAAAGAGCttt                          >  1:1832418/1‑51 (MQ=255)                                                            cAGGTGCCGTTATTTCTTTTATTGATTGTTCGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATg  <  1:298524/69‑1 (MQ=255)                                                            cAGGTGCCGTTATTTCTTTTATTGATTGTTCGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATg  <  1:2879627/69‑1 (MQ=255)                                                            cAGGTGCCGTTATTTCTTTTATTGATTGTTCGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATg  <  1:2521171/69‑1 (MQ=255)                                                            cAGGTGCCGTTATTTCTTTTATTGATTGTTCGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATg  <  1:1524961/69‑1 (MQ=255)                                                             |                                                                    GATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAACAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTCGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATG  >  minE/195580‑195707

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑