| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,317,228 | G→C | 45.5% | A301A (GCG→GCC) | glpX → | fructose 1,6‑bisphosphatase II |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,317,228 | 0 | G | C | 45.5% | 22.6 / 74.3 | 66 | A301A (GCG→GCC) | glpX | fructose 1,6‑bisphosphatase II |
| Reads supporting (aligned to +/- strand): ref base G (24/12); new base C (20/10); total (44/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01 | |||||||||||
TTCTCTGCCACCGGTATTACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTCGCCGCATTCAGTCCATCCACT > minE/2317163‑2317292 | ttctCTGCCACCGGTATTACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGACt > 1:3117930/1‑69 (MQ=255) aCCGGTATTACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGACTACCGAAACg > 1:2896095/1‑69 (MQ=255) ttACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGctgctg > 1:1128635/1‑68 (MQ=255) ttACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGa > 1:1939617/1‑69 (MQ=255) ttACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGa > 1:3162650/1‑69 (MQ=255) ttACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGa > 1:1643029/1‑69 (MQ=255) ttACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGa > 1:1668988/1‑69 (MQ=255) ttACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGa > 1:3057648/1‑69 (MQ=255) ttACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGa > 1:1891104/1‑69 (MQ=255) ttACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGa > 1:687283/1‑69 (MQ=255) ttACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGa > 1:2392654/1‑69 (MQ=255) aCCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGATc > 1:1069149/1‑69 (MQ=255) ctgctgGAAGGCATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCa > 1:1448809/1‑69 (MQ=255) ggAAGGCATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCa < 1:307830/64‑1 (MQ=255) ggAAGGCATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCa < 1:1973879/64‑1 (MQ=255) cATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTc > 1:1129371/1‑68 (MQ=255) aTTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTcgc > 1:2971658/1‑69 (MQ=255) aTTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTcgc > 1:1567090/1‑69 (MQ=255) aTTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTcgc > 1:207581/1‑69 (MQ=255) aTTAGCCGCAAAGGCAATATCGCCACCACCGAACCGCTGCTGATCCGTGGTAAATCACGCACCATTcgt > 1:2750290/1‑68 (MQ=255) aTTAGCCGCAAAGGCAATATCGCCACCACCGAAACGCTGCTGATCCGTGGTAAATCACGCACCATTcgt > 1:1860501/1‑68 (MQ=255) aTTAGCCGCAAAGGCAATATCGCCACCACCGAAACGCTGCTGATCCGTGGTAAATCACGCACCATTcgt > 1:2320237/1‑68 (MQ=255) aTTAGCCGCAAAGGCAATATCGCCACCACCGAAACGCTGCTGATCCGTGGTAAATCACGCACCATTcgt > 1:749649/1‑68 (MQ=255) aTTAGCCGCAAAGGCAATATCGCCACCACCGAAACGCTGCTGATCCGTGGTAAATCACGCACCATTcgt > 1:1994037/1‑68 (MQ=255) aTTAGCCGCAAAGGCAATATCGCCACCACCGAAACGCTGCTGATCCGTGGTAAATCACGCACCATTcgt > 1:1124907/1‑68 (MQ=255) aTTAGCCGCAAAGGCAATATCGCCACCACCGAAACGCTGCTGATCCGTGGTAAATCACGCACCATTcgt > 1:1965462/1‑68 (MQ=255) aTTAGCCGCAAAGGCAATATCGCCACCACCGAAACGCTGCTGATCCGTGGTAAATCACGCACCATTcgt > 1:588358/1‑68 (MQ=255) aTTAGCCGCAAAGGCAATATCGCCACCACCGAAACGCTGCTGATCCGTGGTAAATCACGCACCATTcgt > 1:2640343/1‑68 (MQ=255) aTTAGCCGCAAAGGCAATATCGCCACCACCGAAACGCTGCTGATCCGTGGTAAATCACGCACCATTcgt > 1:1791515/1‑68 (MQ=255) aTTAGCCGCAAAGGCAATATCGCCACCACCGAAACGCTGCTGATCCGTGGTAAATCACGCACCATTcgt > 1:1783734/1‑68 (MQ=255) aTTAGCCGCAAAGGCAATATCGCCACCACCGAAACGCTGCTGATCCGTGGTAAATCACGCACCATTcgt > 1:1752373/1‑68 (MQ=255) aTTAGCCGCAAAGGCAATATCGCCACCACCGAAACGCTGCTGATCCGTGGTAAATCACGCACCATTcgt > 1:477565/1‑68 (MQ=255) aTTAGCCGCAAAGGCAATATCGCCACCACCGAAACGCTGCTGATCCGTGGTAAATCACGCACCATTcgt > 1:3308910/1‑68 (MQ=255) aTTAGCCGCAAAGGCAATATCGCCACCACCGAAACGCTGCTGATCCGTGGTAAATCACGCACCATTcgt > 1:1574794/1‑68 (MQ=255) aTTAGCCGCAAAGGCAATATCGCCACCACCGAAACGCTGCTGATCCGTGGTAAATCACGCACCATTcgt > 1:1588099/1‑68 (MQ=255) aTTAGCCGCAAAGGCAATATCGCCACCACCGAAACGCTGCTGATCCGTGGTAAATCACGCACCATTcgt > 1:1652510/1‑68 (MQ=255) aTTAGCCGCAAAGGCAATATCGCCACCACCGAAACGCTGCTGATCCGTGGTAAATCACGCACCATTcg > 1:585951/1‑68 (MQ=255) aTTAGCCGCAAAGGCAATATCGCCACCACCGAAACGCTGCTGATCCGTGGTAAATCACGCACCATTcg > 1:1268304/1‑68 (MQ=255) aTTAGCCGCAAAGGCAATATCGCCACCACCGAAACGCTGCTGATCCGTGGTAAATCACGCACCATTcg > 1:1076364/1‑68 (MQ=255) gCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTCGCCGCa > 1:865069/1‑60 (MQ=255) gCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTCGCCGCa > 1:1019888/1‑60 (MQ=255) gCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTCGCCGCa < 1:2988054/60‑1 (MQ=255) gCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTCGCCGCa < 1:564207/60‑1 (MQ=255) gCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTCGCCGCa > 1:311636/1‑60 (MQ=255) gCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTCGCCGCa > 1:1048649/1‑60 (MQ=255) gCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTCGCCGCa < 1:1117177/60‑1 (MQ=255) gCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTCGCCGCa < 1:1260210/60‑1 (MQ=255) gCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTCGCCGCa < 1:172526/60‑1 (MQ=255) gCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTCGCCGCa > 1:1421051/1‑60 (MQ=255) gCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTCGCCGCa < 1:2287803/60‑1 (MQ=255) gCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTCGCCGCa > 1:1601868/1‑60 (MQ=255) tatCGCCACCACCGAAACGCTGCTGATCCGTGGTaa < 1:3425534/36‑1 (MQ=16) tatCGCCACCACCGAAACGCTGCTGATCCGTGGTaa < 1:1163130/36‑1 (MQ=16) tatCGCCACCACCGAAACGCTGCTGATCCGTGGTaa < 1:1353095/36‑1 (MQ=16) tatCGCCACCACCGAAACGCTGCTGATCCGTGGTaa < 1:3196160/36‑1 (MQ=16) tatCGCCACCACCGAAACGCTGCTGATCCGTGGTaa < 1:1602253/36‑1 (MQ=16) tatCGCCACCACCGAAACGCTGCTGATCCGTGGTaa < 1:170617/36‑1 (MQ=16) tatCGCCACCACCGAAACGCTGCTGATCCGTGGTaa < 1:1750497/36‑1 (MQ=16) tatCGCCACCACCGAAACGCTGCTGATCCGTGGTaa < 1:1812437/36‑1 (MQ=16) tatCGCCACCACCGAAACGCTGCTGATCCGTGGTaa < 1:3005781/36‑1 (MQ=16) atCGCCACCACCGAAACGCTGCTGATCCGTGGTaa < 1:681831/35‑1 (MQ=16) tCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTCGCCGCATTCAGTCCATCCACt > 1:1565903/1‑69 (MQ=255) tCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTCGCCGCATTCAGTCCATCCACt < 1:1910828/69‑1 (MQ=255) tCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTCGCCGCATTCAGTCCATCCACt < 1:863605/69‑1 (MQ=255) tCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTCGCCGCATTCAGTCCATCCACt < 1:1950333/69‑1 (MQ=255) tCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCAACATTCGCCGCATTCAGTCCATCCACt < 1:447517/69‑1 (MQ=255) | TTCTCTGCCACCGGTATTACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTCGCCGCATTCAGTCCATCCACT > minE/2317163‑2317292 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |