| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,317,540 | T→C | 57.2% | F36F (TTT→TTC) | fpr → | ferredoxin‑NADP reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,317,540 | 0 | T | C | 57.2% | 10.5 / 28.6 | 42 | F36F (TTT→TTC) | fpr | ferredoxin‑NADP reductase |
| Reads supporting (aligned to +/- strand): ref base T (11/7); new base C (5/19); total (16/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.14e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.38e-01 | |||||||||||
CTGGACCGACGCCCTGTTTAGTCTCACCGTTCACGCCCCCGTGCTTCCGTTTACCGCCGGGCAATTTACCAAGCTTGGCCTTGAAATCGACGGCGAACGCGTCCAGCGCGCCTA > minE/2317474‑2317587 | cTGGACCGACGCCCTGTTTAGTCTCACCGTTCACGCCCCCGTGCTTCCGTTTACCGCCGGGCAATTTa > 1:2341710/1‑68 (MQ=255)cTGGACCGACGCCCTGTTTAGTCTCACCGTTCACGCCCCCGTGCTTCCGTTTACCGCCGGGCAATTTa > 1:1326274/1‑68 (MQ=255)cTGGACCGACGCCCTGTTTAGTCTCACCGTTCACGCCCCCGTGCTTCCGTTTACCGCCGGGCAATTTa > 1:2296795/1‑68 (MQ=255)cTGGACCGACGCCCTGTTTAGTCTCACCGTTCACGCCCCCGTGCTTCCGTTTACCGCCGGGCAATTTa > 1:2913653/1‑68 (MQ=255) tAGTCTCACCGTTCACGCCCCCGTGCTTCCGTTTACCGCCGGGCAATTTACCAAGCTTGGCCTTGaa < 1:1043442/67‑1 (MQ=255) tAGTCTCACCGTTCACGCCCCCGTGCTTCCGTTTACCGCCGGGCAATTTACCAAGCTTGGCCTTGaa < 1:143865/67‑1 (MQ=255) tAGTCTCACCGTTCACGCCCCCGTGCTTCCGTTTACCGCCGGGCAATTTACCAAGCTTGGCCTTGaa < 1:2597219/67‑1 (MQ=255) tAGTCTCACCGTTCACGCCCCCGTGCTTCCGTTTACCGCCGGGCAATTTACCAAGCTTGGCCTTGaa < 1:2156255/67‑1 (MQ=255) tAGTCTCACCGTTCACGCCCCCGTGCTTCCGTTTACCGCCGGGCAATTTACCAAGCTTGGCCTTGa < 1:970639/66‑1 (MQ=255) tAGTCTCACCGTTCACGCCCCCGTGCTTCCGTTTACCACCGGGCAATTTACCAAGCTTGGCCTTGa < 1:899101/66‑1 (MQ=255) tcACCGTTCACGCCCCCGTGCTTCCGTTTACCGCCGGGCAATTTACCAAGCTTgg > 1:267459/1‑55 (MQ=255) cGTTCACGCTCCCGTGCTTCCGTTTACCGCCGTGCAATTCACCaaa < 1:525294/46‑2 (MQ=37) cGTTCACGCTCCCGTGCTTCCGTTTACCGCCGGGCAATTCACCaaa < 1:3372703/46‑2 (MQ=38) cGTTCACGCTCCCGTGCTTCCGTTTACCGCCGGGCAATTCACCaaa < 1:3366819/46‑2 (MQ=38) cGTTCACGCTCCCGTGCTTCCGTTTACCGCCGGGCAATTCACCaaa < 1:3211916/46‑2 (MQ=38) cGTTCACGCTCCCGTGCTTCCGTTTACCGCCGGGCAATTCACCaaa < 1:3063995/46‑2 (MQ=38) cGTTCACGCTCCCGTGCTTCCGTTTACCGCCGGGCAATTCACCaaa < 1:466535/46‑2 (MQ=38) cGTTCACGCTCCCGTGCTTCCGTTTACCGCCGGGCAATTCACCaaa < 1:828616/46‑2 (MQ=38) cGTTCACGCTCCCGTGCTTCCGTTTACCGCCGGGCAATTCACCaaa < 1:947502/46‑2 (MQ=38) cGTTCACGCTCCCGTGCTTCCGTTTACCGCCGGGCAATTCACCaaa < 1:2444518/46‑2 (MQ=38) cGTTCACGCTCCCGTGCTTCCGTTTACCGCCGGGCAATTCACCaaa < 1:1218662/46‑2 (MQ=38) cGTTCACGCTCCCGTGCTTCCGTTTACCGCCGGGCAATTCACCaaa < 1:1503073/46‑2 (MQ=38) cGTTCACGCTCCCGTGCTTCCGTTTACCGCCGGGCAATTCACCaaa < 1:1947659/46‑2 (MQ=38) cGTTCACGCTCCCGTGCTTCCGTTTACCGCCGGGCAATTCACCaaa < 1:1939657/46‑2 (MQ=38) cGTTCACGCTCCCGTGCTTCCGTTTACCGCCGGGCAATTCACCaaa < 1:1667374/46‑2 (MQ=38) cGTTCACGCTCCCGTGCTTCCGTTTACAGCCGGGCAATTCACCaaa < 1:2083129/46‑2 (MQ=255) gTTCACGCTCCCGTGCTTCCGTTTACCGCCGGGCAATTCACCaaa < 1:619692/45‑2 (MQ=38) gTTCACGCTCCCGTGCTTCCGTTTACCGCCGGGCAATTCACCaaa < 1:336115/45‑2 (MQ=38) gTTCACGCTCCCGTGCTTCCGTTTACCGCCGGGCAATTCACCaaa < 1:1768504/45‑2 (MQ=38) cGCTCCCGTGCTTCCGTTTACCGCCGGGCAATTCACCaaa < 1:3160765/40‑2 (MQ=37) cccGTGCTTCCGTTTACCGCCGGGCAATTTACCAAGCTTGGCCTTGAAATCGACGGCGAACGCGTCCAg < 1:2407012/69‑1 (MQ=255) gCTTCCGTTTACCGCCGGGCAATTTACCAAGCTTGGCCTTGAAATCGACGGCGAACGCGTcc > 1:3187053/1‑62 (MQ=255) gCTTCCGTTTACCGCCGGGCAATTTACCAAGCTTGGCCTTGAAATCGACGGCGAACGCGTCCAgcgcg > 1:1044414/1‑68 (MQ=255) gCTTCCGTTTACCGCCGGGCAATTTACCAAGCTTGGCCTTGAAATCGACGGCGAACGCGTCCAgcgcg > 1:1495831/1‑68 (MQ=255) gCTTCCGTTTACCGCCGGGCAATTTACCAAGCTTGGCCTTGAAATCGACGGCGAACGCGTCCAgcgcg > 1:1560991/1‑68 (MQ=255) gCTTCCGTTTACCGCCGGGCAATTTACCAAGCTTGGCCTTGAAATCGACGGCGAACGCGTCCAgcgcg > 1:2843219/1‑68 (MQ=255) gCTTCCGTTTACCGCCGGGCAATTTACCAAGCTTGGCCTTGAAATCGACGGCGAACGCGTCCAgcgcg > 1:2171867/1‑68 (MQ=255) tCCGTTTACCGCCGGGCAATTCACCAAACTTGGCCTTGAAATTGATGGCGACCGCGTTCAGCGCGCCt > 1:2343843/1‑68 (MQ=255) tCCGTTTACCGCCGGGCAATTCACCAAACTTGGCCTTGAAATTGATGGCGAACGCGTTCAGCGCGCCTa > 1:1691265/1‑69 (MQ=255) tCCGTTTACCGCCGGGCAATTCACCAAACTTGGCCTTGAAATTGATGGCGAACGCGTTCAGCGCGCCTa > 1:3122918/1‑69 (MQ=255) tCCGTTTACCGCCGGGCAATTCACCAAACTTGGCCTTGAAATTGATGGCGAACGCGTTCAGCGCGCCTa > 1:78127/1‑69 (MQ=255) tCCGTTTACCGCCGGGCAATTCACCAAACTTGGCCTTGAAATTGATGGCGAACGCGTTCAGCGCGCCTa > 1:2210337/1‑69 (MQ=255) | CTGGACCGACGCCCTGTTTAGTCTCACCGTTCACGCCCCCGTGCTTCCGTTTACCGCCGGGCAATTTACCAAGCTTGGCCTTGAAATCGACGGCGAACGCGTCCAGCGCGCCTA > minE/2317474‑2317587 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |