| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,317,828 | T→A | 36.3% | D132E (GAT→GAA) | fpr → | ferredoxin‑NADP reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,317,828 | 0 | T | A | 36.3% | 53.9 / 52.1 | 66 | D132E (GAT→GAA) | fpr | ferredoxin‑NADP reductase |
| Reads supporting (aligned to +/- strand): ref base T (30/12); new base A (16/8); total (46/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.83e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GATGCTGGCAACCGGTACAGCGATTGGCCCTTATTTATCGATTCTGCAACTAGGTAAAGATTTAGATCGCTTCAAAAATCTGGTCCTGGTGCACGCCGCACGTTATGCCGCCGACTTAAGCTAT > minE/2317762‑2317885 | gATGCTGGCAACCGGTACAGCGACTGGCCCTTATTTATCGATTCTGCAACTAGGTAAAGATTTAGATCg > 1:73442/1‑69 (MQ=255) aaCCGGTACAGCGATTGGCCCTTATTTATCGATTCTGCAACTAGGTAAAGATTTAGATCGCTTCaaaa < 1:1140596/68‑1 (MQ=255) aCCGGTACAGCGATTGGCCCTTATTTATCGATTCTGCAACTAGGTAAAGATTTAGATCGCTTCaaaa < 1:989171/67‑1 (MQ=255) ccGGTACAGCGATTGGCCCTTATTTATCGATTCTGCAATTGGGCAAAGATTTAGAACGCTTCaaaaaca > 1:380373/1‑67 (MQ=255) ccGGTACAGCGATTGGCCCTTATTTATCGATTCTGCAATTGGGCAAAGATTTAGAACGCTTCaaaaaca > 1:224569/1‑67 (MQ=255) ccGGTACAGCGATTGGCCCTTATTTATCGATTCTGCAATTGGGCAAAGATTTAGAACGCTTCaaaaaca > 1:2300130/1‑67 (MQ=255) ccGGTACAGCGATTGGCCCTTATTTATCGATTCTGCAATTGGGCAAAGATTTAGAACGCTTCaaaaaca > 1:2735227/1‑67 (MQ=255) ccGGTACAGCGATTGGCCCTTATTTATCGATTCTGCAATTGGGCAAAGATTTAGAACGCTTCaaaaaca > 1:2773687/1‑67 (MQ=255) ccGGTACAGCGATTGGCCCTTATTTATCGATTCTGCAATTGGGCAAAGATTTAGAACGCTTCaaaaaca > 1:2800931/1‑67 (MQ=255) ccGGTACAGCGATTGGCCCTTATTTATCGATTCTGCAATTGGGCAAAGATTTAGAACGCTTCaaaaaca > 1:3111335/1‑67 (MQ=255) ccGGTACAGCGATTGGCCCTTATTTATCGATTCTGCAATTGGGCAAAGATTTAGAACGCTTCaaaaaca > 1:3222868/1‑67 (MQ=255) ccGGTACAGCGATTGGCCCTTATTTATCGATTCTGCAATTGGGCAAAGATTTAGAACGCTTCaaaaaca > 1:1775845/1‑67 (MQ=255) ccGGTACAGCGATTGGCCCTTATTTATCGATTCTGCAATTGGGCAAAGATTTAGAACGCTTCaaaaaca > 1:1749680/1‑67 (MQ=255) ccGGTACAGCGATTGGCCCTTATTTATCGATTCTGCAATTGGGCAAAGATTTAGAACGCTTCaaaaaca > 1:1597167/1‑67 (MQ=255) ccGGTACAGCGATTGGCCCTTATTTATCGATTCTGCAATTGGGCAAAGATTTAGAACGCTTCaaaaaca > 1:3243082/1‑67 (MQ=255) ccGGTACAGCGATTGGCCCTTATTTATCGATTCTGCAATTGGGCAAAGATTTAGAACGCTTCaaaaaca > 1:1375030/1‑67 (MQ=255) ccGGTACAGCGATTGGCCCTTATTTATCGATTCTGCAATTGGGCAAAGATTTAGAACGCTTCaaaaaca > 1:671474/1‑67 (MQ=255) ccGGTACAGCGATTGGCCCTTATTTATCGATTCTGCAATTGGGCAAAGATTTAGAACGCTTCaaaaaca > 1:733390/1‑67 (MQ=255) ccGGTACAGCGATTGGCCCTTATTTATCGATTCTGCAATTGGGCAAAGATTTAGAACGCTTCaaaaaca > 1:994642/1‑67 (MQ=255) ggTACAGCGATTGGCCCTTATTTATCGATTCTGCAACTAGGTAAAGATTTAGATCGCTTCAAAAATCTg > 1:27183/1‑69 (MQ=255) ggTACAGCGATTGGCCCTTATTTATCGATTCTGCAACTAGGTAAAGATTTAGATCGCTTCAAAAATCTg > 1:427503/1‑69 (MQ=255) ggTACAGCGATTGGCCCTTATTTATCGATTCTGCAACTAGGTAAAGATTTAGATCGCTTCAAAAATCTg > 1:592615/1‑69 (MQ=255) ggTACAGCGATTGGCCCTTATTTATCGATTCTGCAACTAGGTAAAGATTTAGATCGCTTCAAAAATCTg > 1:966535/1‑69 (MQ=255) ggTACAGCGATTGGCCCTTATTTATCGATTCTGCAACTAGGTAAAGATTTAGATCGCTTCAAAAATCTg > 1:1372444/1‑69 (MQ=255) ggTACAGCGATTGGCCCTTATTTATCGATTCTGCAACTAGGAAAAGATTTAGATAGCTTCAAAAATCTg > 1:2395396/1‑69 (MQ=255) gCGATTGGCCCTTATTTATCTATTCTGCAACTAGGTAAAGATTTAGATCGCTTCAAAAATCTGGTCCTg > 1:1209520/1‑69 (MQ=255) gCGATTGGCCCTTATTTATCGATTCTGCAACTAGGTAAAGATTTAGATCGCTTCAAAAATCTGGTCCTg > 1:2948257/1‑69 (MQ=255) gCGATTGGCCCTTATTTATCGATTCTGCAACTAGGTAAAGATTTAGATCGCTTCAAAAATCTGGTCCTg > 1:531863/1‑69 (MQ=255) gCGATTGGCCCTTATTTATCGATTCTGCAACTAGGTAAAGATTTAGATCGCTTCAAAAATCTGGTCCTg > 1:1005162/1‑69 (MQ=255) cGATTGGCCCTTATTTATCGATTCTGCAACTAGGTAAAGATTTAGATCGCTTCAAAAATCTGGTCCTgg > 1:3077145/1‑69 (MQ=255) cGATTGGCCCTTATTTATCGATTCTGCAACTAGGTAAAGATTTAGATCGCTTCAAAAATCTGGTCCTgg > 1:1992299/1‑69 (MQ=255) cGATTGGCCCTTATTTATCGATTCTGCAACTAGGTAAAGATTTAGATCGCTTCAAAAATCTGGTCCTgg > 1:3093184/1‑69 (MQ=255) atCGATTCTGCAACTAGGTAAAGATTTAGATCGCTTCAAAAATCTGGTCCTGGTGCACGCCGCACGTTa > 1:2914300/1‑69 (MQ=255) atCGATTCTGCAACTAGGTAAAGATTTAGATCGCTTCAAAAATCTGGTCCTGGTGCACGCCGCACGTTa > 1:563574/1‑69 (MQ=255) atCGATTCTGCAACTAGGTAAAGATTTAGATCGCTTCAAAAATCTGGTCCTGGTGCACGCCGCACGTTa > 1:12587/1‑69 (MQ=255) atCGATTCTGCAACTAGGTAAAGATTTAGATCGCTTCAAAAATCTGGTCCTGGTGCACGCCGCACGTTa > 1:2342095/1‑69 (MQ=255) atCGATTCTGCAACTAGGTAAAGATTTAGATCGCTTCAAAAATCTGGTCCTGGTGCACGCCGCACGTTa > 1:760025/1‑69 (MQ=255) atCGATTCTGCAACTAGGTAAAGATTTAGATCGCTTCAAAAATCTGGTCCTGGTGCACGCCGCACGTTa > 1:1572085/1‑69 (MQ=255) atCGATTCTGCAACTAGGTAAAGATTTAGATCGCTTCAAAAATCTGGTCCTGGTGCACGCCGCACGTTa > 1:3277940/1‑69 (MQ=255) gATTCTGCAACTAGGTAAAGATTTAGATCGCTTCAAAAATCTGGTCCTGGTGCACGCCGCACGTTAt < 1:1580328/67‑1 (MQ=255) gATTCTGCAACTAGGTAAAGATTTAGATCGCTTCAAAAATCTGGGCCTGGTGCACGCCGCACGTTAt < 1:1074884/67‑1 (MQ=255) aGGTAAAGATTTAGATCGCTTCAAAAATCTGGTCCTGGTGCACGCCGCACGTTATGCCGCCGACTTaa > 1:1838501/1‑68 (MQ=255) aaaGATTTAGATCGCTTCAAAAATCTGGTCCTGGTGCACGCCGCACGTTATGCCGCCGACTTAAGCTAt > 1:3215020/1‑69 (MQ=255) aaGATTTAGATCGCTTCAAAAATCTGGTCCTGGTGCACGCCGCACGt > 1:1493684/1‑47 (MQ=255) aaGATTTAGATCGCTTCAAAAATCTGGTCCTGGTGCACGCCGCACGt > 1:1314148/1‑47 (MQ=255) aaGATTTAGATCGCTTCAAAAATCTGGTCCTGGTGCACGCCGCACGt < 1:93731/47‑1 (MQ=255) aaGATTTAGATCGCTTCAAAAATCTGGTCCTGGTGCACGCCGCACGt < 1:1200940/47‑1 (MQ=255) aaGATTTAGATCGCTTCAAAAATCTGGTCCTGGTGCACGCCGCACGt < 1:1875895/47‑1 (MQ=255) aaGATTTAGATCGCTTCAAAAATCTGGTCCTGGTGCACGCCGCACGt > 1:117061/1‑47 (MQ=255) aaGATTTAGATCGCTTCAAAAATCTGGTCCTGGTGCACGCCGCACGt > 1:1912651/1‑47 (MQ=255) aaGATTTAGATCGCTTCAAAAATCTGGTCCTGGTGCACGCCGCACGt > 1:2742182/1‑47 (MQ=255) aaGATTTAGATCGCTTCAAAAATCTGGTCCTGGTGCACGCCGCACGt < 1:269514/47‑1 (MQ=255) aaGATTTAGATCGCTTCAAAAATCTGGTCCTGGTGCACGCCGCACGt < 1:2606258/47‑1 (MQ=255) aaGATTTAGATCGCTTCAAAAATCTGGTCCTGGTGCACGCCGCACGt > 1:2479190/1‑47 (MQ=255) aaGATTTAGATCGCTTCAAAAATCTGGTCCTGGTGCACGCCGCACGt < 1:2370228/47‑1 (MQ=255) aaGATTTAGATCGCTTCAAAAATCTGGTCCTGGTGCACGCCGCACGt < 1:2294237/47‑1 (MQ=255) aaGATTTAGATCGCTTCAAAAATCTGGTCCTGGTGCACGCCGCACGt > 1:2122417/1‑47 (MQ=255) aGATTTAGATCGCTTCAAAAATCTGGTCCTGGTGCACGCCGCACGt < 1:745568/46‑1 (MQ=255) aGATTTAGAACGCTTCAAAAACATGGTGCTGGTTCACGCCGCACGATATGCCGCCGACTTaa < 1:3292693/62‑1 (MQ=255) aGATTTAGAACGCTTCAAAAACATGGTGCTGGTTCACGCCGCACGATATGCCGCCGACTTaa < 1:3292918/62‑1 (MQ=255) aGATTTAGAACGCTTCAAAAACATGGTGCTGGTTCACGCCGCACGATATGCCGCCGACTTaa < 1:3411581/62‑1 (MQ=255) aGATTTAGAACGCTTCAAAAACATGGTGCTGGTTCACGCCGCACGATATGCCGCCGACTTaa < 1:192325/62‑1 (MQ=255) aGATTTAGAACGCTTCAAAAACATGGTGCTGGTTCACGCCGCACGATATGCCGCCGACTTaa < 1:2009800/62‑1 (MQ=255) aGAATTAGAACGCTTCAAAAACATGGTGCTGGTTCACCCCGCACGATATGCCGCCGACTTaa < 1:2086308/62‑1 (MQ=255) gATTTAGAACGCTTCAAAAACATGGTGCTGGTTCACGCCGCACGATATGCCGCCGACTTaa < 1:3162947/61‑1 (MQ=255) aTTTAGAACGCTTCAAAAACATGGTGCTGGTTCACGCCGCACGATATGCCGCCGACTTaa < 1:2780711/60‑1 (MQ=255) | GATGCTGGCAACCGGTACAGCGATTGGCCCTTATTTATCGATTCTGCAACTAGGTAAAGATTTAGATCGCTTCAAAAATCTGGTCCTGGTGCACGCCGCACGTTATGCCGCCGACTTAAGCTAT > minE/2317762‑2317885 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |