BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,318,211	C→G	33.1%	L134F (TTG→TTC) ‡	yiiT ←	stress‑induced protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,318,211	0	C	G	33.1%	132.6 / 98.0	127	L134F (TTG→TTC) ‡	yiiT	stress‑induced protein
Reads supporting (aligned to +/- strand): ref base C (35/50); new base G (24/18); total (59/68)
Fisher's exact test for biased strand distribution p-value = 1.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.57e-01

Only 100 of 128 total aligned reads displayed.

TGACAGCGGAGCATTACTGGTAAGCGGTTACTTATCGATAAACGGCACGATGAGCAAATCCGCACTCATCTTATTGATCATCCCGCGATATGCCGGCATCAAACGGTTGATAAATGAGTGAT  >  minE/2318157‑2318278

tGACAGCGGAGCATTACTGGTAAGCGGTTACTTGTCGATAAACGGCACGATCAAGAGATCCGCACTCaa                                                       >  1:2188824/1‑68 (MQ=255)

tGACAGCGGAGCATTACTGGTAAGCGGTTACTTGTCGATAAACGGCACGATCAAGAGATCCGCACTCaa                                                       >  1:3376532/1‑68 (MQ=255)

tGACAGCGGAGCATTACTGGTAAGCGGTTACTTGTCGATAAACGGCACGATCAAGAGATCCGCACTCaa                                                       >  1:2004207/1‑68 (MQ=255)

tGACAGCGGAGCATTACTGGTAAGCGGTTACTTGTCGATAAACGGCACGATCAAGAGATCCGCACTCaa                                                       >  1:564966/1‑68 (MQ=255)

tGACAGCGGAGCATTACTGGTAAGCGGTTACTTGTCGATAAACGGCACGATCAAGAGATCCGCACTCaa                                                       >  1:1968154/1‑68 (MQ=255)

tGACAGCGGAGCATTACTGGTAAGCGGTTACTTGTCGATAAACGGCACGATCAAGAGATCCGCACTCaa                                                       >  1:2812690/1‑68 (MQ=255)

tGACAGCGGAGCATTACTGGTAAGCGGTTACTTGTCGATAAACGGCACGATCAAGAGATCCGCACTCaa                                                       >  1:1938670/1‑68 (MQ=255)

tGACAGCGGAGCATTACTGGTAAGCGGTTACTTGTCGATAAACGGCACGATCAAGAGATCCGCACTCaa                                                       >  1:2582260/1‑68 (MQ=255)

tGACAGCGGAGCATTACTGGTAAGCGGTTACTTGTCGATAAACGGCACGATCAAGAGATCCGCACTCaa                                                       >  1:38755/1‑68 (MQ=255)

tGACAGCGGAGCATTACTGGTAAGCGGTTACTTGTCGATAAACGGCACGATCAAGAGATCCGCACTCaa                                                       >  1:2654892/1‑68 (MQ=255)

tGACAGCGGAGCATTACTGGTAAGCGGTTACTTGTCGATAAACGGCACGATCAAGAGATACGCACTCaa                                                       >  1:3274868/1‑68 (MQ=255)

tGACAGCGGAGCATTACTGGTAAGCGGTTACTTATCGATAAACGGCACGATGAGCAAATCCGCACTCAt                                                       >  1:1625997/1‑69 (MQ=255)

tGACAGCGGAGCATTACTGGTAAGCGGTTACTTATCGATAAACGGCACGATGAGCAAATCCGCACTCAt                                                       >  1:2325765/1‑69 (MQ=255)

tGACAGCGGAGCATTACTGGTAAGCGGTTACTTATCGATAAACGGCACGATGAGCAAATCCGCACTCAt                                                       >  1:1843542/1‑69 (MQ=255)

tGACAGCGGAGCATTACTGGTAAGCGGTTACTTATCGATAAACGGCACGATGAGCAAATCCGCACTCAt                                                       >  1:558360/1‑69 (MQ=255)

tGACAGCGGAGCATTACTGGTAAGCGGTTACTTATCGATAAACGGCACGATGAGCAAATCCGCACTCAt                                                       >  1:2392176/1‑69 (MQ=255)

tGACAGCGGAGCATTACTGGTAAGCGGTTACTTATCGATAAACGGCACGATGAGCAAATCCGCACTCAt                                                       >  1:2097293/1‑69 (MQ=255)

tGACAGCGGAGCATTACTGGTAAGCGGTTACTTATCGATAAACGGCACGATGAGCAAATCCGCACTCAt                                                       >  1:212330/1‑69 (MQ=255)

tGACAGCGGAGCATTACTGGTAAGCGGTTACTTATCGATAAACGGCACGATGAGCAAATCCGCACTCAt                                                       >  1:776519/1‑69 (MQ=255)

tGACAGCGGAGCATTACTGGTAAGCGGTTACTTATCGATAAACGGCACGATGAGCAAATCCGCACTCAt                                                       >  1:3248113/1‑69 (MQ=255)

tGACAGCGGAGCATTACTGGTAAGCGGTTACTTATCGATAAACGGCACGATGAGCAAATCCGCACTCAt                                                       >  1:2795155/1‑69 (MQ=255)

tGACAGCGGAGCATTACTGGTAAGCGGTTACTTATCGATAAACGGCACGATGAGCAAATACGCACTCAt                                                       >  1:3366452/1‑69 (MQ=255)

tGACAGCGGAGCATTACTGGTAAGCGGTGACTTATCGATAAACGGCACGATGAGCAAATCCGCACTCa                                                        >  1:1447297/1‑68 (MQ=255)

    aGCGGAGCATTACTGGTAAGCGGTTACTTATCGATAAACGGCACGATGAGCAAATCCGCACTCATCTTa                                                   <  1:1432227/69‑1 (MQ=255)

        gAGCATTACTGGTAAGCGGTTACTTATCGATAAACGGCACGATGAGCAAATCCGCACTCATCTTATTGa                                               <  1:203813/69‑1 (MQ=255)

         aGCATTACTGGTAAGCGGTTACTTATCGATAAACGGCACGATGAGCAAATCCGCACTCATCTTATTGat                                              <  1:1885328/69‑1 (MQ=255)

         aGCATTACTGGTAAGCGGTTACTTATCGATAAACGGCACGATGAGCAAATCCGCACTCATCTTATTGat                                              <  1:2991962/69‑1 (MQ=255)

         aGCATTACTGGTAAGCGGTTACTTATCGATAAACGGCACGATGAGCAAATCCGCACTCATCTTATTGat                                              <  1:979244/69‑1 (MQ=255)

               aCTGGTAAGCGGTTACTTATCGATAAACGGCACGATGAGCaa                                                                   >  1:958562/1‑42 (MQ=255)

               aCTGGTAAGCGGTTACTTATCGATAAACGGCACGATGAGCAAATCCGCACTCATCTTATTGTTCATccc                                        >  1:1185307/1‑69 (MQ=255)

               aCTGGTAAGCGGTTACTTATCGATAAACGGCACGATGAGCAAATCCGCACTCATCTTATTGATCATccc                                        >  1:2436504/1‑69 (MQ=255)

               aCTGGTAAGCGGTTACTTATCGATAAACGGCACGATGAGCAAATCCGCACTCATCTTATTGATCATccc                                        >  1:563942/1‑69 (MQ=255)

               aCTGGTAAGCGGTTACTTATCGATAAACGGCACGATGAGCAAATCCGCACTCATCTTATTGATCATccc                                        >  1:1135818/1‑69 (MQ=255)

               aCTGGTAAGCGGTTACTTATCGATAAACGGCACGATGAGCAAATCCGCACTCATCTTATTGATCATccc                                        >  1:2012536/1‑69 (MQ=255)

               aCTGGTAAGCGGTTACTTATCGATAAACGGCACGATGAGCAAATCCGCACTCATCTTATTGATCATccc                                        >  1:1708931/1‑69 (MQ=255)

               aCTGGTAAGCGGTTACTTATCGATAAACGGCACGATGAGCAAATCCGCACTCATCTTATTGATCATccc                                        >  1:2341770/1‑69 (MQ=255)

               aCTGGTAAGCGGTTACTTATCGATAAACGGCACGATGAGCAAATCCGCACTCATCTTATTGATCATccc                                        >  1:1045051/1‑69 (MQ=255)

               aCTGGTAAGCGGTTACTTATCGATAAACGGCACGATGAGCAAATCCGCACTCATCTTATTGATCATcc                                         >  1:991363/1‑68 (MQ=255)

               aCTGGTAAGCGGTTACTTATCGATAAACGGCACGATGAGCAAATCCGCACTCATCTTATTGATCATcc                                         >  1:1460590/1‑68 (MQ=255)

                  ggTAAGCGGTTACTTATCGATAAACGGCACGATGAGCAAATCCGCACTCATCTTATTGATCATCCcgc                                      <  1:3025806/68‑1 (MQ=255)

                  ggTAAGCGGTTACTTATCGATAAACGGCACGATGAGCAAATCCGCACTCATCTTATTGATCATCCcgc                                      <  1:241202/68‑1 (MQ=255)

                  ggTAAGCGGTTACTTATCGATAAACGGCACGATGAGCAAATCCGCACTCATCTTATTGATCATCCcgc                                      <  1:1896067/68‑1 (MQ=255)

                  ggTAAGCGGTTACTTATCGATAAACGGCACGATGAGCAAATCCGCACTCATCTTATTGATCATCCcgc                                      <  1:1298341/68‑1 (MQ=255)

                  ggTAAGCGGTTACTTATCGATAAACGGCACGATGAGCAAATCCGCACTCATCTTATTGATCATCCcgc                                      <  1:893472/68‑1 (MQ=255)

                  ggTAAGCGGATACTTATCGATAAACGGCACGATGAGCAAATCCGCACTCATCTTATTGATCATCCcgc                                      <  1:99010/68‑1 (MQ=255)

                   gTAAGCGGTTACTTGTCGATAAACGGCACGATCAAGAGATCCGCACTCAACTTATTGATCATCCcgcg                                     >  1:3099240/1‑68 (MQ=255)

                   gTAAGCGGTTACTTGTCGATAAACGGCACGATCAAGAGATCCGCACTCAACTTATTGATCATCCCGCGa                                    >  1:1813008/1‑69 (MQ=255)

                   gTAAGCGGTTACTTGTCGATAAACGGCACGATCAAGAGATCCGCACTCAACTTATTGATCATCCCGCGa                                    >  1:3143246/1‑69 (MQ=255)

                   gTAAGCGGTTACTTGTCGATAAACGGCACGATCAAGAGATCCGCACTCAACTTATTGATCATCCCGCGa                                    >  1:3360639/1‑69 (MQ=255)

                   gTAAGCGGTTACTTGTCGATAAACGGCACGATCAAGAGATCAGCACTCAACTTATTGATCATCCCGCGa                                    >  1:2516997/1‑69 (MQ=255)

                   gTAAGCGGATACTTGTCGATAAACGGCACGATCAAGAGATCCGCACTCAACTTATTGATCATCCCGCGa                                    >  1:1546877/1‑69 (MQ=255)

                     aaGCGGTTACTTATCGATAAACGGCACGATGAGCAAATCCGCACTCATCTTATTGATCATCCCGCGa                                    <  1:2052908/67‑1 (MQ=255)

                     aaGCGGTTACTTATCGATAAACGGCACGATGAGCAAATCCGCACTCATCTTATTGATCATCCCGCGa                                    <  1:1281162/67‑1 (MQ=255)

                     aaGCGGTTACTTATCGATAAACGGCACGATGAGCAAATCCGCACTCATCTTATTGATCATCCCGCGa                                    <  1:1373795/67‑1 (MQ=255)

                     aaGCGGTTACTTATCGATAAACGGCACGATGAGCAAATCCGCACTCATCTTATTGATCATCCCGCGa                                    <  1:71199/67‑1 (MQ=255)

                     aaGCGGTTACTTATCGATAAACGGCACGATGAGCAAATCCGCACTCATCTTATTGATCATCCCGCGa                                    <  1:643074/67‑1 (MQ=255)

                     aaGCGGTTACTTATCGATAAACGGCACGATGAGCAAATCCGCACTCATCTTATTGATCATCCCGCGa                                    <  1:591391/67‑1 (MQ=255)

                     aaGCGGTTACTTATCGATAAACGGCACGATGAGCAAATCCGCACTCATCTTATTGATCATCCCGCGa                                    <  1:512472/67‑1 (MQ=255)

                      aGCGGTTACTTATCGATAAACGGCACGATGAGCAAATCCGCACTCATCTTATTGATCATCCCGCGa                                    <  1:2425916/66‑1 (MQ=255)

                          gTTACTTGTCGATAAACGGCACGATCAAGAGATCCGCACTCAACTTATTGTTCATCCCGCGATATGCCg                             <  1:2254602/69‑1 (MQ=255)

                          gTTACTTGTCGATAAACGGCACGATCAAGAGATCCGCACTCAACTTATTGATCATCCCGCGATATGCCg                             <  1:3233883/69‑1 (MQ=255)

                          gTTACTTGTCGATAAACGGCACGATCAAGAGATCCGCACTCAACTTATTGATCATCCCGCGATATGCCg                             <  1:384003/69‑1 (MQ=255)

                          gTTACTTGTCGATAAACGGCACGATCAAGAGATCCGCACTCAACTTATTGATCATCCCGCGATATGCCg                             <  1:3128580/69‑1 (MQ=255)

                          gTTACTTGTCGATAAACGGCACGATCAAGAGATCCGCACTCAACTTATTGATCATCCCGCGATATGCCg                             <  1:459810/69‑1 (MQ=255)

                          gTTACTTGTCGATAAACGGCACGATCAAGAGATCCGCACTCAACTTATTGATCATCCCGCGATATGCCg                             <  1:1029141/69‑1 (MQ=255)

                          gTTACTTGTCGATAAACGGCACGATCAAGAGATCCGCACTCAACTTATTGATCATCCCGCGATATGCCg                             <  1:1310622/69‑1 (MQ=255)

                          gTTACTTGTCGATAAACGGCACGATCAAGAGATCCGCACTCAACTTATTGATCATCCCGCGATATGCCg                             <  1:1418964/69‑1 (MQ=255)

                          gTTACTTGTCGATAAACGGCACGATCAAGAGATCCGCACTCAACTTATTGATCATCCCGCGATATGCCg                             <  1:1423617/69‑1 (MQ=255)

                          gTTACTTGTCGATAAACGGCACGATCAAGAGATCCGCACTCAACTTATTGATCATCCCGCGATATGCCg                             <  1:1726714/69‑1 (MQ=255)

                          gTTACTTGTCGATAAACGGCACGATCAAGAGATCCGCACTCAACTTATTGATCATCCCGCGATATGCCg                             <  1:1873406/69‑1 (MQ=255)

                          gTTACTTGTCGATAAACGGCACGATCAAGAGATCCGCACTCAACTTATTGATCATCCCGCGATATGCCg                             <  1:2622665/69‑1 (MQ=255)

                          gTTACTTGTCGATAAACGGCACGATCAAGAGATCCGCACTCAACTTATTGATCATCCCGCGATATGCCg                             <  1:279447/69‑1 (MQ=255)

                          gTTACTTGTCGATAAACGGCACGATCAAGAGATCCGCACTCAACTTATTGATCATCCCGCGATATGCCg                             <  1:2732019/69‑1 (MQ=255)

                          gTTACTTGTCGATAAACGGCACGATCAAGAGATCCGCACTCAACTTATTGATCATCCCGCGATATGCCg                             <  1:2696547/69‑1 (MQ=255)

                             aCTTATCGATAAACGGCACGATGAGCAAATCCGCACTCATCTTATTGATCATCCCGCGATATGCCGGc                           >  1:726889/1‑68 (MQ=255)

                             aCTTATCGATAAACGGCACGATGAGCAAATCCGCACTCATCTTATTGATCATCCCGCGATATGCCGGc                           >  1:2529629/1‑68 (MQ=255)

                             aCTTATCGATAAACGGCACGATGAGCAAATCCGCACTCATCTTATTGATCATCCCGCGATATGCCGGc                           >  1:1705759/1‑68 (MQ=255)

                                tATCGATAAACGGCACGATGAGCAAATCCGCACTCATCTTATTGATCATCCCGCGa                                    <  1:1235985/56‑1 (MQ=255)

                                tATCGATAAACGGCACGATGAGCAAATCCGCACTCATCTTATTGATCATCCCGCGa                                    <  1:580748/56‑1 (MQ=255)

                                tATCGATAAACGGCACGATGAGCAAATCCGCACTCATCTTATTGATCATCCCGCGa                                    <  1:1084975/56‑1 (MQ=255)

                                 aTCGATAAACGGCACGATGAGCAAATCCGCACTCATCTTATTGATCATCCCGCGATATGCCGGCATCaa                      >  1:1799699/1‑69 (MQ=255)

                                 aTCGATAAACGGCACGATGAGCAAATCCGCACTCATCTTATTGATCATCCCGCGATATGCCGGCATCaa                      >  1:622222/1‑69 (MQ=255)

                                 aTCGATAAACGGCACGATGAGCAAATCCGCACTCATCTTATTGATCATCCCGCGATATGCCGGCATCaa                      >  1:2561588/1‑69 (MQ=255)

                                 aTCGATAAACGGCACGATGAGCAAATCCGCACTCATCTTATTGATCATCCCGCGATATGCCGGCATCaa                      >  1:2574453/1‑69 (MQ=255)

                                          cGGCACGATGAGCAAATCCGCACTCATCTTATTGat                                              <  1:2477096/36‑1 (MQ=255)

                                             cACGATGAGCAAATC‑‑CACTCATCTTATTGATCATCCCGCGa                                    <  1:87237/41‑1 (MQ=37)

                                                  tGAGCAAATCCGCACTCATCTTATTGATCATCCCGCGATATGCCGGCATCAAACGGTTGATAAATGAGt     <  1:1004454/69‑1 (MQ=255)

                                                  tGAGCAAATCCGCACTCATCTTATTGATCATCCCGCGATATGCCGGCATCAAACGGTTGATAAATGAGt     <  1:802510/69‑1 (MQ=255)

                                                  tGAGCAAATCCGCACTCATCTTATTGATCATCCCGCGATATGCCGGCATCAAACGGTTGATAAATGAGt     <  1:1368414/69‑1 (MQ=255)

                                                  tGAGCAAATCCGCACTCATCTTATTGATCATCCCGCGATATGCCGGCATCAAACGGTTGATAAATGAGt     <  1:1290304/69‑1 (MQ=255)

                                                  tGAGCAAATCCGCACTCATCTTATTGATCATCCCGCGATATGCCGGCATCAAACGGTTGATAAATGAGt     <  1:299599/69‑1 (MQ=255)

                                                  tGAGCAAATCCGCACTCATCTTATTGATCATCCCGCGATATGCCGGCATCAAACGGTTGATAAATGAGt     <  1:667947/69‑1 (MQ=255)

                                                  tGAGCAAATCCGCACTCATCTTATTGATCATCCCGCGATATGCCGGCATCAAACGGTTGATAAATGAGt     <  1:585790/69‑1 (MQ=255)

                                                  tGAGCAAATCCGCACTCATCTTATTGATCATCCCGCGATATGCCGGCATCAAACGGTTGATAAATGAGt     <  1:583092/69‑1 (MQ=255)

                                                  tGAGCAAATCCGCACTCATCTTATTGATCATCCCGCGATATGCCGGCATCAAACGGTTGATAAATGAGt     <  1:1863545/69‑1 (MQ=255)

                                                  tGAGCAAATCCGCACTCATCTTATTGATCATCCCGCGATATGCCGGCATCAAACGGTTGATAAATGAGt     <  1:475358/69‑1 (MQ=255)

                                                  tGAGCAAATCCGCACTCATCTTATTGATCATCCCGCGATATGCCGGCATCAAACGGTTGATAAATGAGt     <  1:1992895/69‑1 (MQ=255)

                                                  tGAGCAAATCCGCACTCATCTTATTGATCATCCCGCGATATGCCGGCATCAAACGGTTGATAAATGAGt     <  1:2585740/69‑1 (MQ=255)

                                                  tGAGCAAATCCGCACTCATCTTATTGATCATCCCGCGATATGCCGGCATCAAACGGTTGATAAATGAGt     <  1:3194878/69‑1 (MQ=255)

                                                      cAAATCCGCACTCATCTTATTGATCATCCCGCGATATGCCGGCATCAAACGGTTGATAAATGAGtgat  <  1:2917229/68‑1 (MQ=255)

TGACAGCGGAGCATTACTGGTAAGCGGTTACTTATCGATAAACGGCACGATGAGCAAATCCGCACTCATCTTATTGATCATCCCGCGATATGCCGGCATCAAACGGTTGATAAATGAGTGAT  >  minE/2318157‑2318278

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`