BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,318,225	T→A	25.0%	M130L (ATG→TTG)	yiiT ←	stress‑induced protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,318,225	0	T	A	25.0%	155.3 / 52.8	100	M130L (ATG→TTG)	yiiT	stress‑induced protein
Reads supporting (aligned to +/- strand): ref base T (21/54); new base A (6/19); total (27/73)
Fisher's exact test for biased strand distribution p-value = 7.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

Only 100 of 111 total aligned reads displayed.

TGACAGCGGAGCATTACTGGTAAGCGGTTACTTATCGATAAACGGCACGATGAGCAAATCCGCACTCATCTTATTGATCATCCCGCGATATGCCGGCATCAAACGGTTGATAAATGAGTGATGATGAC  >  minE/2318157‑2318284

tGACAGCGGAGCATTACTGGTAAGCGGTTACTTATCGATAAACGGCACGATGAGCAAATCCGCACTCAt                                                             >  1:558360/1‑69 (MQ=255)

tGACAGCGGAGCATTACTGGTAAGCGGTTACTTATCGATAAACGGCACGATGAGCAAATCCGCACTCAt                                                             >  1:2795155/1‑69 (MQ=255)

tGACAGCGGAGCATTACTGGTAAGCGGTTACTTATCGATAAACGGCACGATGAGCAAATCCGCACTCAt                                                             >  1:3248113/1‑69 (MQ=255)

tGACAGCGGAGCATTACTGGTAAGCGGTTACTTATCGATAAACGGCACGATGAGCAAATCCGCACTCAt                                                             >  1:2392176/1‑69 (MQ=255)

tGACAGCGGAGCATTACTGGTAAGCGGTTACTTATCGATAAACGGCACGATGAGCAAATCCGCACTCAt                                                             >  1:2325765/1‑69 (MQ=255)

tGACAGCGGAGCATTACTGGTAAGCGGTTACTTATCGATAAACGGCACGATGAGCAAATCCGCACTCAt                                                             >  1:212330/1‑69 (MQ=255)

tGACAGCGGAGCATTACTGGTAAGCGGTTACTTATCGATAAACGGCACGATGAGCAAATCCGCACTCAt                                                             >  1:1843542/1‑69 (MQ=255)

tGACAGCGGAGCATTACTGGTAAGCGGTTACTTATCGATAAACGGCACGATGAGCAAATCCGCACTCAt                                                             >  1:1625997/1‑69 (MQ=255)

tGACAGCGGAGCATTACTGGTAAGCGGTTACTTATCGATAAACGGCACGATGAGCAAATCCGCACTCAt                                                             >  1:776519/1‑69 (MQ=255)

    aGCGGAGCATTACTGGTAAGCGGTTACTTATCGATAAACGGCACGATGAGCAAATCCGCACTCATCTTa                                                         <  1:3157704/69‑1 (MQ=255)

    aGCGGAGCATTACTGGTAAGCGGTTACTTATCGATAAACGGCACGATGAGCAAATCCGCACTCATCTTa                                                         <  1:702766/69‑1 (MQ=255)

    aGCGGAGCATTACTGGTAAGCGGTTACTTATCGATAAACGGCACGATGAGCAAATCCGCACTCATCTTa                                                         <  1:1432227/69‑1 (MQ=255)

        gAGCATTACTGGTAAGCGGTTACTTATCGATAAACGGCACGATGAGCAAATCCGCACTCATCTTATTGa                                                     <  1:203813/69‑1 (MQ=255)

         aGCATTACTGGTAAGCGGTTACTTATCGATAAACGGCACGATGAGCAAATCCGCACTCATCTTATTGat                                                    <  1:2991962/69‑1 (MQ=255)

         aGCATTACTGGTAAGCGGTTACTTATCGATAAACGGCACGATGAGCAAATCCGCACTCATCTTATTGat                                                    <  1:1885328/69‑1 (MQ=255)

         aGCATTACTGGTAAGCGGTTACTTATCGATAAACGGCACGATGAGCAAATCCGCACTCATCTTATTGat                                                    <  1:979244/69‑1 (MQ=255)

         aGCATTACTGGTAAGCGGTTACTTATCGATAAACGGCACGATGAGCAAATCCGCACTCATCTTATTGat                                                    <  1:589522/69‑1 (MQ=255)

               aCTGGTAAGCGGTTACTTATCGATAAACGGCACGATGAGCAAATCCGCACTCATCTTATTGTTCATccc                                              >  1:1185307/1‑69 (MQ=255)

               aCTGGTAAGCGGTTACTTATCGATAAACGGCACGATGAGCAAATCCGCACTCATCTTATTGATCATccc                                              >  1:1818323/1‑69 (MQ=255)

               aCTGGTAAGCGGTTACTTATCGATAAACGGCACGATGAGCAAATCCGCACTCATCTTATTGATCATccc                                              >  1:563942/1‑69 (MQ=255)

               aCTGGTAAGCGGTTACTTATCGATAAACGGCACGATGAGCAAATCCGCACTCATCTTATTGATCATccc                                              >  1:1708931/1‑69 (MQ=255)

               aCTGGTAAGCGGTTACTTATCGATAAACGGCACGATGAGCAAATCCGCACTCATCTTATTGATCATccc                                              >  1:2012536/1‑69 (MQ=255)

               aCTGGTAAGCGGTTACTTATCGATAAACGGCACGATGAGCAAATCCGCACTCATCTTATTGATCATccc                                              >  1:1135818/1‑69 (MQ=255)

               aCTGGTAAGCGGTTACTTATCGATAAACGGCACGATGAGCAAATCCGCACTCATCTTATTGATCATccc                                              >  1:2341770/1‑69 (MQ=255)

               aCTGGTAAGCGGTTACTTATCGATAAACGGCACGATGAGCAAATCCGCACTCATCTTATTGATCATccc                                              >  1:1045051/1‑69 (MQ=255)

               aCTGGTAAGCGGTTACTTATCGATAAACGGCACGATGAGCAAATCCGCACTCATCTTATTGATCATccc                                              >  1:259645/1‑69 (MQ=255)

               aCTGGTAAGCGGTTACTTATCGATAAACGGCACGATGAGCAAATCCGCACTCATCTTATTGATCATcc                                               >  1:991363/1‑68 (MQ=255)

               aCTGGTAAGCGGTTACTTATCGATAAACGGCACGATGAGCAAATCCGCACTCATCTTATTGATCATcc                                               >  1:1460590/1‑68 (MQ=255)

                  ggTAAGCGGTTACTTATCGATAAACGGCACGATGAGCAAATCCGCACTCATCTTATTGATCATCCcgc                                            <  1:241202/68‑1 (MQ=255)

                  ggTAAGCGGTTACTTATCGATAAACGGCACGATGAGCAAATCCGCACTCATCTTATTGATCATCCcgc                                            <  1:1896067/68‑1 (MQ=255)

                  ggTAAGCGGTTACTTATCGATAAACGGCACGATGAGCAAATCCGCACTCATCTTATTGATCATCCcgc                                            <  1:893472/68‑1 (MQ=255)

                  ggTAAGCGGATACTTATCGATAAACGGCACGATGAGCAAATCCGCACTCATCTTATTGATCATCCcgc                                            <  1:99010/68‑1 (MQ=255)

                   gTAAGCGGTTACTTGTCGATAAACGGCACGATCAAGAGATCCGCACTCAACTTATTGATCATCCcgcg                                           >  1:3099240/1‑68 (MQ=255)

                   gTAAGCGGTTACTTGTCGATAAACGGCACGATCAAGAGATCCGCACTCAACTTATTGATCATCCCGCGa                                          >  1:3143246/1‑69 (MQ=255)

                   gTAAGCGGTTACTTGTCGATAAACGGCACGATCAAGAGATCCGCACTCAACTTATTGATCATCCCGCGa                                          >  1:1813008/1‑69 (MQ=255)

                   gTAAGCGGTTACTTGTCGATAAACGGCACGATCAAGAGATCCGCACTCAACTTATTGATCATCCCGCGa                                          >  1:3360639/1‑69 (MQ=255)

                   gTAAGCGGTTACTTGTCGATAAACGGCACGATCAAGAGATCAGCACTCAACTTATTGATCATCCCGCGa                                          >  1:2516997/1‑69 (MQ=255)

                   gTAAGCGGATACTTGTCGATAAACGGCACGATCAAGAGATCCGCACTCAACTTATTGATCATCCCGCGa                                          >  1:1546877/1‑69 (MQ=255)

                     atgCGGTTACTTATCGATAAACGGCACGATGAGCAAATCCGCACTCATCTTATTGATCATCCCGCGa                                          <  1:789469/65‑1 (MQ=255)

                     aaGCGGTTACTTATCGATAAACGGCACGATGAGCAAATCCGCACTCATCTTATTGATCATCCCGCGa                                          <  1:2379561/67‑1 (MQ=255)

                     aaGCGGTTACTTATCGATAAACGGCACGATGAGCAAATCCGCACTCATCTTATTGATCATCCCGCGa                                          <  1:1311216/67‑1 (MQ=255)

                     aaGCGGTTACTTATCGATAAACGGCACGATGAGCAAATCCGCACTCATCTTATTGATCATCCCGCGa                                          <  1:71199/67‑1 (MQ=255)

                     aaGCGGTTACTTATCGATAAACGGCACGATGAGCAAATCCGCACTCATCTTATTGATCATCCCGCGa                                          <  1:1373795/67‑1 (MQ=255)

                     aaGCGGTTACTTATCGATAAACGGCACGATGAGCAAATCCGCACTCATCTTATTGATCATCCCGCGa                                          <  1:1281162/67‑1 (MQ=255)

                     aaGCGGTTACTTATCGATAAACGGCACGATGAGCAAATCCGCACTCATCTTATTGATCATCCCGCGa                                          <  1:2820287/67‑1 (MQ=255)

                     aaGCGGTTACTTATCGATAAACGGCACGATGAGCAAATCCGCACTCATCTTATTGATCATCCCGCGa                                          <  1:643074/67‑1 (MQ=255)

                     aaGCGGTTACTTATCGATAAACGGCACGATGAGCAAATCCGCACTCATCTTATTGATCATCCCGCGa                                          <  1:591391/67‑1 (MQ=255)

                     aaGCGGTTACTTATCGATAAACGGCACGATGAGCAAATCCGCACTCATCTTATTGATCATCCCGCGa                                          <  1:2052908/67‑1 (MQ=255)

                     aaGCGGTTACTTATCGATAAACGGCACGATGAGCAAATCCGCACTCATCTTATTGATCATCCCGCGa                                          <  1:512472/67‑1 (MQ=255)

                     aaGCGGTTACTTATCGATAAACGGCACGATGAGCAAATCCGCACTCATCTTATTGATCATCCCGCGa                                          <  1:568853/67‑1 (MQ=255)

                      aGCGGTTACTTATCGATAAACGGCACGATGAGCAAATCCGCACTCATCTTATTGATCATCCCGCGa                                          <  1:2425916/66‑1 (MQ=255)

                          gTTACTTGTCGATAAACGGCACGATCAAGAGATCCGCACTCAACTTATTGTTCATCCCGCGATATGCCg                                   <  1:2254602/69‑1 (MQ=255)

                          gTTACTTGTCGATAAACGGCACGATCAAGAGATCCGCACTCAACTTATTGATCATCCCGCGATATGCCg                                   <  1:3233883/69‑1 (MQ=255)

                          gTTACTTGTCGATAAACGGCACGATCAAGAGATCCGCACTCAACTTATTGATCATCCCGCGATATGCCg                                   <  1:3128580/69‑1 (MQ=255)

                          gTTACTTGTCGATAAACGGCACGATCAAGAGATCCGCACTCAACTTATTGATCATCCCGCGATATGCCg                                   <  1:384003/69‑1 (MQ=255)

                          gTTACTTGTCGATAAACGGCACGATCAAGAGATCCGCACTCAACTTATTGATCATCCCGCGATATGCCg                                   <  1:459810/69‑1 (MQ=255)

                          gTTACTTGTCGATAAACGGCACGATCAAGAGATCCGCACTCAACTTATTGATCATCCCGCGATATGCCg                                   <  1:1029141/69‑1 (MQ=255)

                          gTTACTTGTCGATAAACGGCACGATCAAGAGATCCGCACTCAACTTATTGATCATCCCGCGATATGCCg                                   <  1:1726714/69‑1 (MQ=255)

                          gTTACTTGTCGATAAACGGCACGATCAAGAGATCCGCACTCAACTTATTGATCATCCCGCGATATGCCg                                   <  1:1310622/69‑1 (MQ=255)

                          gTTACTTGTCGATAAACGGCACGATCAAGAGATCCGCACTCAACTTATTGATCATCCCGCGATATGCCg                                   <  1:204170/69‑1 (MQ=255)

                          gTTACTTGTCGATAAACGGCACGATCAAGAGATCCGCACTCAACTTATTGATCATCCCGCGATATGCCg                                   <  1:1418964/69‑1 (MQ=255)

                          gTTACTTGTCGATAAACGGCACGATCAAGAGATCCGCACTCAACTTATTGATCATCCCGCGATATGCCg                                   <  1:1873406/69‑1 (MQ=255)

                          gTTACTTGTCGATAAACGGCACGATCAAGAGATCCGCACTCAACTTATTGATCATCCCGCGATATGCCg                                   <  1:1423617/69‑1 (MQ=255)

                          gTTACTTGTCGATAAACGGCACGATCAAGAGATCCGCACTCAACTTATTGATCATCCCGCGATATGCCg                                   <  1:2622665/69‑1 (MQ=255)

                          gTTACTTGTCGATAAACGGCACGATCAAGAGATCCGCACTCAACTTATTGATCATCCCGCGATATGCCg                                   <  1:279447/69‑1 (MQ=255)

                          gTTACTTGTCGATAAACGGCACGATCAAGAGATCCGCACTCAACTTATTGATCATCCCGCGATATGCCg                                   <  1:2732019/69‑1 (MQ=255)

                           ttACTTGTCGATAAACGGCACGATCAAGAGATCCGCACTCAACTTATTGATCATCCCGCGATATGCCg                                   <  1:2251773/68‑1 (MQ=255)

                             aCTTATCGATAAACGGCACGATGAGCAAATCCGCACTCATCTTATTGATCATCCCGCGATATGCCGGc                                 >  1:1705759/1‑68 (MQ=255)

                             aCTTATCGATAAACGGCACGATGAGCAAATCCGCACTCATCTTATTGATCATCCCGCGATATGCCGGc                                 >  1:2529629/1‑68 (MQ=255)

                             aCTTATCGATAAACGGCACGATGAGCAAATCCGCACTCATCTTATTGATCATCCCGCGATATGCCGGc                                 >  1:278587/1‑68 (MQ=255)

                                tATCGATAAACGGCACGATGAGCAAATCCGCACTCATCTTATTGATCATCCCGCGa                                          <  1:3059815/56‑1 (MQ=255)

                                tATCGATAAACGGCACGATGAGCAAATCCGCACTCATCTTATTGATCATCCCGCGa                                          <  1:1235985/56‑1 (MQ=255)

                                tATCGATAAACGGCACGATGAGCAAATCCGCACTCATCTTATTGATCATCCCGCGa                                          <  1:1084975/56‑1 (MQ=255)

                                 aTCGATAAACGGCACGATGAGCAAATCCGCACTCATCTTATTGATCATCCCGCGATATGCCGGCATCaa                            >  1:622222/1‑69 (MQ=255)

                                 aTCGATAAACGGCACGATGAGCAAATCCGCACTCATCTTATTGATCATCCCGCGATATGCCGGCATCaa                            >  1:1799699/1‑69 (MQ=255)

                                 aTCGATAAACGGCACGATGAGCAAATCCGCACTCATCTTATTGATCATCCCGCGATATGCCGGCATCaa                            >  1:2561588/1‑69 (MQ=255)

                                 aTCGATAAACGGCACGATGAGCAAATCCGCACTCATCTTATTGATCATCCCGCGATATGCCGGCATCaa                            >  1:2574453/1‑69 (MQ=255)

                                          cGGCACGATGAGCAAATCCGCACTCATCTTATTGat                                                    <  1:2477096/36‑1 (MQ=255)

                                             cACGATGAGCAAATC‑‑CACTCATCTTATTGATCATCCCGCGa                                          <  1:87237/41‑1 (MQ=37)

                                               cGATGAGCAAATCCGCACTCATCTTATTGATCATCCcgc                                            <  1:961573/39‑1 (MQ=255)

                                                  tGAGCAAATCCGCACTCATCTTATTGATCATCCCGCGATATGCCGGCATCAAACGGTTGATAAATGAGt           <  1:1290304/69‑1 (MQ=255)

                                                  tGAGCAAATCCGCACTCATCTTATTGATCATCCCGCGATATGCCGGCATCAAACGGTTGATAAATGAGt           <  1:802510/69‑1 (MQ=255)

                                                  tGAGCAAATCCGCACTCATCTTATTGATCATCCCGCGATATGCCGGCATCAAACGGTTGATAAATGAGt           <  1:1368414/69‑1 (MQ=255)

                                                  tGAGCAAATCCGCACTCATCTTATTGATCATCCCGCGATATGCCGGCATCAAACGGTTGATAAATGAGt           <  1:667947/69‑1 (MQ=255)

                                                  tGAGCAAATCCGCACTCATCTTATTGATCATCCCGCGATATGCCGGCATCAAACGGTTGATAAATGAGt           <  1:1092822/69‑1 (MQ=255)

                                                  tGAGCAAATCCGCACTCATCTTATTGATCATCCCGCGATATGCCGGCATCAAACGGTTGATAAATGAGt           <  1:1004454/69‑1 (MQ=255)

                                                  tGAGCAAATCCGCACTCATCTTATTGATCATCCCGCGATATGCCGGCATCAAACGGTTGATAAATGAGt           <  1:1424321/69‑1 (MQ=255)

                                                  tGAGCAAATCCGCACTCATCTTATTGATCATCCCGCGATATGCCGGCATCAAACGGTTGATAAATGAGt           <  1:585790/69‑1 (MQ=255)

                                                  tGAGCAAATCCGCACTCATCTTATTGATCATCCCGCGATATGCCGGCATCAAACGGTTGATAAATGAGt           <  1:583092/69‑1 (MQ=255)

                                                  tGAGCAAATCCGCACTCATCTTATTGATCATCCCGCGATATGCCGGCATCAAACGGTTGATAAATGAGt           <  1:475358/69‑1 (MQ=255)

                                                  tGAGCAAATCCGCACTCATCTTATTGATCATCCCGCGATATGCCGGCATCAAACGGTTGATAAATGAGt           <  1:1992895/69‑1 (MQ=255)

                                                  tGAGCAAATCCGCACTCATCTTATTGATCATCCCGCGATATGCCGGCATCAAACGGTTGATAAATGAGt           <  1:3194878/69‑1 (MQ=255)

                                                  tGAGCAAATCCGCACTCATCTTATTGATCATCCCGCGATATGCCGGCATCAAACGGTTGATAAATGAGt           <  1:2547539/69‑1 (MQ=255)

                                                  tGAGCAAATCCGCACTCATCTTATTGATCATCCCGCGATATGCCGGCATCAAACGGTTGATAAATGAGt           <  1:2585740/69‑1 (MQ=255)

                                                  tGAGCAAATCCGCACTCATCTTATTGATCATCCCGCGATATGCCGGCATCAAACGGTTGATAAATGAGt           <  1:299599/69‑1 (MQ=255)

                                                      cAAATCCGCACTCATCTTATTGATCATCCCGCGATATGCCGGCATCAAACGGTTGATAAATGAGtgat        <  1:2917229/68‑1 (MQ=255)

                                                           ccGCACTCATCTTATTGATCATCCCGCGATATGCCGGCATCAAACGGTTGATAAATGAGTGATGATGAc  <  1:1983913/69‑1 (MQ=255)

                                                           ccGCACTCATCTTATTGATCATCCCGCGATATGCCGGCATCAAACGGTTGATAAATGAGTGATGATGAc  <  1:457617/69‑1 (MQ=255)

                                                           ccGCACTCATCTTATTGATCATCCCGCGATATGCCGGCATCAAACGGTTGATAAATGAGTGATGATGAc  <  1:2835425/69‑1 (MQ=255)

                                                            cGCACTCAACTTATTGATCATCCCGCGATATGCCg                                   <  1:517430/35‑1 (MQ=255)

TGACAGCGGAGCATTACTGGTAAGCGGTTACTTATCGATAAACGGCACGATGAGCAAATCCGCACTCATCTTATTGATCATCCCGCGATATGCCGGCATCAAACGGTTGATAAATGAGTGATGATGAC  >  minE/2318157‑2318284

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`