| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,321,376 | T→C | 34.6% | V200V (GTA→GTG) | cdh ← | CDP‑diacylglycerol phosphotidylhydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,321,376 | 0 | T | C | 34.6% | 53.0 / 43.9 | 78 | V200V (GTA→GTG) | cdh | CDP‑diacylglycerol phosphotidylhydrolase |
| Reads supporting (aligned to +/- strand): ref base T (37/14); new base C (14/13); total (51/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.32e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.02e-01 | |||||||||||
ACAAATGAGTTATCACTCTGCCGTACCATCGCCAGCCCGTAGCGTCCCATATGTTCCCGCGCCTCAGGTACTTCTTCTGCCAGCATCATAAATGGGCTGCGTTGTACCAGTTCGCTTTCCGTTACCCGACGCGCC > minE/2321308‑2321442 | aCAAATGAGTTATCACTCTGCCGTACCATCGCCAGCCCGTAGCGTCCCATATGTTCCCGCGCCTCAGGt > 1:334006/1‑69 (MQ=255) cAAATGAGTTATCACTCTGCCGTACCATCGCCAGCCCGTAGCGTCCCATATGTTCCCGCGCCTCAGGTa > 1:2565032/1‑69 (MQ=255) cAAATGAGTTATCACTCTGCCGTACCATCGCCAGCCCGTAGCGTCCCATATGTTCCCGCGCCTCAGGTa > 1:2011660/1‑69 (MQ=255) cAAATGAGTTATCACTCTGCCGTACCATCGCCAGCCCGTAGCGTCCCATATGTTCCCGCGCCTCAGGTa > 1:2955067/1‑69 (MQ=255) cAAATGAGTTATCACTCTGCCGTACCATCGCCAGCCCGTAGCGTCCCATATGTTCCCGCGCCTCAGGTa > 1:3156586/1‑69 (MQ=255) cAAATGAGTTATCACTCTGCCGTACCATCGCCAGCCCGTAGCGTCCCATATGTTCCCGCGCCTCAGGTa > 1:1640833/1‑69 (MQ=255) cAAATGAGTTATCACTCTGCCGTACCATCGCCAGCCCGTAGCGTCCCATATGTTCCCGCGCCTCAGGTa > 1:144560/1‑69 (MQ=255) tGAGTTATCACTCTGCCGTACCATCGCCAGCCCGTAGCGTCCCATATGTTCCCGCGCCTCAGGTActtc > 1:2357496/1‑69 (MQ=255) tGAGTTATCACTCTGCCGTACCATCGCCAGCCCGTAGCGTCCCATATGTTCCCGCGCCTCAGGTActtc > 1:1196582/1‑69 (MQ=255) tGAGTTATCACTCTGCCGTACCATCGCCAGCCCGTAGCGTCCCATATGTTCCCGCGCCTCAGGTActtc > 1:167610/1‑69 (MQ=255) tGAGTTATCACTCTGCCGTACCATCGCCAGCCCGTAGCGTCCCATATGTTCCCGCGCCTCAGGTActtc > 1:3292916/1‑69 (MQ=255) tGAGTTATCACTCTGCCGTACCATCGCCAGCCCGTAGCGTCCCATATGTTCCCGCGCCTCAGGTActt > 1:1559799/1‑68 (MQ=255) ctGCCGTACCATCGCCAGCCCGTAGCGTCCCATATGTTCCCGCGCCTCAGGTACTTCTTCTGCCAGcat < 1:1010635/69‑1 (MQ=255) ctGCCGTACCATCGCCAGCCCGTAGCGTCCCATATGTTCCCGCGCCTCAGGTACTTCTTCTGCCAGcat < 1:2497268/69‑1 (MQ=255) ctGCCGTACCATCGCCAGCCCGTAGCGTCCCATATGTTCCCGCGCCTCAGGTACTTCTTCTGCCAGcat < 1:1832501/69‑1 (MQ=255) ccATCGCCAGCCCGTAGCGTCCCATATGTTCCCGCGCCTCAGGTACTTCTTCTGCCAGCATCATAAAt > 1:1490559/1‑68 (MQ=255) ccATCGCCAGCCCGTAGCGTCCCATATGTTCCCGCGCCTCAGGTACTTCTTCTGCCAGCATCATAAATg > 1:2674131/1‑69 (MQ=255) ccATCGCCAGCCCGTAGCGTCCCATATGTTCCCGCGCCTCAGGTACTTCTTCTGCCAGCATCATAAATg > 1:487657/1‑69 (MQ=255) ccATCGCCAGCCCGTAGCGTCCCATATGTTCCCGCGCCTCAGGTACTTCTTCTGCCAGCATCATAAATg > 1:420988/1‑69 (MQ=255) aTCGCCAGCCCGTAGCGTCCCATATGTTCCCGCGCCTCAGGTACTTCTTCTGCCAGCATCATAAATggg > 1:633812/1‑69 (MQ=255) aTCGCCAGCCCGTAGCGTCCCATATGTTCCCGCGCCTCAGGTACTTCTTCTGCCAGCATCATAAATggg > 1:1247582/1‑69 (MQ=255) cAGCCCGTAGCGTCCCATATGTTCCCGCGCCTCAGGTACTTCTTCTGCCAGCATCATAAATGGGCTGCg > 1:1590753/1‑69 (MQ=255) cAGCCCGTAGCGTCCCATATGTTCCCGCGCCTCAGGTACTTCTTCTGCCAGCATCATAAATGGGCTGCg > 1:141419/1‑69 (MQ=255) aGCCCGTAGCTTCCCATATGTTCCCGCGCCTCAGGCACTTCTTCCGCCAGCATCATaaa > 1:2992349/1‑59 (MQ=255) aGCCCGTAGCTTCCCATATGTTCCCGCGCCTCAGGCACTTCTTCCGCCAGCATCATAAATGGGCTGCGt > 1:2650792/1‑69 (MQ=255) aGCGTCCCATATGTTCCCGCGCCTCAGGTACTTCTTCTGCCAGCATCATAAATGGGCTGCGtt > 1:885909/1‑63 (MQ=255) aGCGTCCCATATGTTCCCGCGCCTCAGGTACTTCTTCTGCCAGCATCATAAATGGGCTGCGTTg > 1:3087405/1‑64 (MQ=255) aGCGTCCCATATGTTCCCGCGCCTCAGGTACTTCTTCTGCCAGCATCATAAATGGGCTGCGTTg > 1:3219899/1‑64 (MQ=255) aGCGTCCCATATGTTCCCGCGCCTCAGGTACTTCTTCTGCCAGCATCATAAATGGGCTGCGTTg > 1:3193586/1‑64 (MQ=255) aGCGTCCCATATGTTCCCGCGCCTCAGGTACTTCTTCTGCCAGCATCATAAATGGGCTGCGTTg > 1:2673465/1‑64 (MQ=255) aGCGTCCCATATGTTCCCGCGCCTCAGGTACTTCTTCTGCCAGCATCATAAATGGGCTGCGTTg > 1:781128/1‑64 (MQ=255) aGCGTCCCATATGTTCCCGCGCCTCAGGTACTTCTTCTGCCAGCATCATAAATGGGCTGCGTTg > 1:2378737/1‑64 (MQ=255) aGCGTCCCATATGTTCCCGCGCCTCAGGTACTTCTTCTGCCAGCATCATAAATGGGCTGCGTTg > 1:1082960/1‑64 (MQ=255) aGCGTCCAATATGTTCCCGCGCCTCAGGTACTTCTTCTGCCAGCATCATAAATGGGCTGCGTTg > 1:3039529/1‑64 (MQ=255) tCCCATATGTTCCCGCGCCTCAGGCACTTCTTCCGCCAGCATCATAAATGGGCTGCGTTGTAcc > 1:2573139/1‑64 (MQ=255) tCCCATATGTTCCCGCGCCTCAGGCACTTCTTCCGCCAGCATCATAAATGGGCTGCGTTGTACCAGtt > 1:207651/1‑68 (MQ=255) tCCCATATGTTCCCGCGCCTCAGGCACTTCTTCCGCCAGCATCATAAATGGGCTGCGTTGTACCAGTTc > 1:915751/1‑69 (MQ=255) tCCCATATGTTCCCGCGCCTCAGGCACTTCTTCCGCCAGCATCATAAATGGGCTGCGTTGTACCAGTTc > 1:39915/1‑69 (MQ=255) tCCCATATGTTCCCGCGCCTCAGGCACTTCTTCCGCCAGCATCATAAATGGGCTGCGTTGTACCAGTTc > 1:1787286/1‑69 (MQ=255) tCCCATATGTTCCCGCGCCTCAGGCACTTCTTCCGCCAGCATCATAAATGGGCTGCGTTGTACCAGTTc > 1:651453/1‑69 (MQ=255) tCCCATATGTTCCCGCGCCTCAGGCACTTCTTCCGCCAGCATCATAAATGGGCTGCGTTGTACCAGTTc > 1:2583885/1‑69 (MQ=255) tCCCATATGTTCCCGCGCCTCAGGCACTTCTTCCGCCAGCATCATAAATGGGCTGCGTTGTACCAGTTc > 1:2499849/1‑69 (MQ=255) tCCCATATGTTCCCGCGCCTCAGGCACTTCTTCCGCCAGCATCATAAATGGGCTGCGTTGTACCAGTTc > 1:2134333/1‑69 (MQ=255) tCCCATATGTTCCCGCGCCTCAGGCACTTCTTCCGCCAGCATCATAAATGGGCTGCGTTGTACCAGTTc > 1:2380685/1‑69 (MQ=255) tCCCATATGTTCCCGCGCCTCAAGCACTTCTTCCGCCAGCATCATAAATGGGCTGCGTTGTACCAGTTc > 1:159385/1‑69 (MQ=255) tCCAATATGTTCCCGCGCCTCAGGCACTTCTTCCGCCAGCATCATa > 1:2241052/1‑46 (MQ=255) atGTTCCCGCGCCTCAGGTACTTCTTCTGCCAGCATCATAAATGGGCTGCGTTGTACCAGTTCGCTTTc > 1:807078/1‑69 (MQ=255) tGTTCCCGCGCCTCAGGTACTTCTTCTGCCAGCATCATa < 1:1392191/39‑1 (MQ=255) tGTTCCCGCGCCTCAGGTACTTCTTCTGCCAGCATCATa < 1:3313722/39‑1 (MQ=255) tGTTCCCGCGCCTCAGGTACTTCTTCTGCCAGCATCATa < 1:1218312/39‑1 (MQ=255) tGTTCCCGCGCCTCAGGTACTTCTTCTGCCAGCATCATa < 1:136979/39‑1 (MQ=255) tGTTCCCGCGCCTCAGGTACTTCTTCTGCCAGCATCATa < 1:2210830/39‑1 (MQ=255) tGTTCCCGCGCCTCAGGTACTTCTTCTGCCAGCATCATa < 1:778200/39‑1 (MQ=255) tGTTCCCGCGCCTCAGGTACTTCTTCTGCCAGCATCATa < 1:2852036/39‑1 (MQ=255) tGTTCCCGCGCCTCAGGTACTTCTTCTGCCAGCATCATa < 1:260452/39‑1 (MQ=255) tGTTCCCGCGCCTCAGGTACTTCTTCTGCCAGCATCATAAATGGGCTGCGTTGTACCAGTTCGCTTTcc > 1:1114926/1‑69 (MQ=255) tGTTCCCGCGCCTCAGGTACTTCTTCTGCCAGCATCATAAATGGGCTGCGTTGTACCAGTTCGCTTTcc > 1:2374702/1‑69 (MQ=255) tGTTCCCGCGCCTCAGGTACTTCTTCTGCCAGCATCATAAATGGGCTGCGTTGTACCAGTTCGCTTTcc > 1:2854855/1‑69 (MQ=255) tGTTCCCGCGCCTCAGGTACTTCTTCTGCCAGCATCATAAATGGGCTGCGTTGTACCAGTTCGCTTTcc > 1:1648344/1‑69 (MQ=255) tGTTCCCGCGCCTCAGGTACTTCTTCTGCCAGCATCATAAATGGGCTGCGTTGTACCAGTTCGCTTTcc > 1:512162/1‑69 (MQ=255) tGTTCCCGCGCCTCAGGTACTTCTTCTGCCAGCATCATAAATGGGCTGCGTTGTACCAGTTCGCTTTcc > 1:1187551/1‑69 (MQ=255) tGTTCCCGCGCCTCAGGTACTTCTTCTGCCAGCATCATAAATGGGCTGCGTTGTACCAGTTCGCTTTcc > 1:2067098/1‑69 (MQ=255) tGTTCCCGCGCCTCAGGTACTTCTTCTGCCAGCATCATAAATGGGCTGCGTTGTACCAGTTCGCTTTcc > 1:3257549/1‑69 (MQ=255) ttCCCGCGCCTCAGGTACTTCTTCTGCCAGCATCATAAATGGGCTGCGTTGTACCAGTTCGCTTTCCGt < 1:2949235/69‑1 (MQ=255) ttCCCGCGCCTCAGGCACTTCTTCCGCCAGCATCATAAATGGGCTGCGTTGTACCAGTTCGCTTTCCGt < 1:1665727/69‑1 (MQ=255) ttCCCGCGCCTCAGGCACTTCTTCCGCCAGCATCATAAATGGGCTGCGTTGTACCAGTTCGCTTTCCGt < 1:1295046/69‑1 (MQ=255) ttCCCGCGCCTCAGGCACTTCTTCCGCCAGCATCATAAATGGGCTGCGTTGTACCAGTTCGCTTTCCGt < 1:1210120/69‑1 (MQ=255) ttCCCGCGCCTCAGGCACTTCTTCCGCCAGCATCATAAATGGGCTGCGTTGTACCAGTTCGCTTTCCGt < 1:801580/69‑1 (MQ=255) ttCCCGCGCCTCAGGCACTTCTTCCGCCAGCATCATAAATGGGCTGCGTTGTACCAGTTCGCTTTCCGt < 1:1426683/69‑1 (MQ=255) ttCCCGCGCCTCAGGCACTTCTTCCGCCAGCATCATAAATGGGCTGCGTTGTACCAGTTCGCTTTCCGt < 1:3197372/69‑1 (MQ=255) ttCCCGCGCCTCAGGCACTTCTTCCGCCAGCATCATAAATGGGCTGCGTTGTACCAGTTCGCTTTCCGt < 1:3182236/69‑1 (MQ=255) ttCCCGCGCCTCAGGCACTTCTTCCGCCAGCATCATAAATGGGCTGCGTTGTACCAGTTCGCTTTCCGt < 1:3130913/69‑1 (MQ=255) ttCCCGCGCCTCAGGCACTTCTTCCGCCAGCATCATAAATGGGCTGCGTTGTACCAGTTCGCTTTCCGt < 1:2944089/69‑1 (MQ=255) ttCCCGCGCCTCAGGCACTTCTTCCGCCAGCATCATAAATGGGCTGCGTTGTACCAGTTCGCTTTCCGt < 1:1996764/69‑1 (MQ=255) ttCCCGCGCCTCAGGCACTTCTTCCGCCAGCATCATAAATGGGCTGCGTTGTACCAGTTCGCTTTCCGt < 1:2502114/69‑1 (MQ=255) ttCCCGCGCCTCAGGCACTTCTTCCGCCAGCATCATAAATGGGCTGCGTTGTACCAGTTCGCTTTCCGt < 1:2304793/69‑1 (MQ=255) ttCCCGCGCCTCAGGCACTTCTTCCGCCAGCATCATAAATGGGCTGCGTTGTACAAGTTCGCTTTCCGt < 1:2136106/69‑1 (MQ=255) ccGCGCCTCAGGTACTTCTTCTGCCAGCATCATAAATGGGCTGCGTTGTACCAGTTCGCTTTCCGtt < 1:1964907/67‑1 (MQ=255) gTACTTCTTCTGCCAGCATCATAAATGGGCTGCGTTGTACCAGTTCGCTTTCCGTTACCCGACGCGcc < 1:2078769/68‑1 (MQ=255) | ACAAATGAGTTATCACTCTGCCGTACCATCGCCAGCCCGTAGCGTCCCATATGTTCCCGCGCCTCAGGTACTTCTTCTGCCAGCATCATAAATGGGCTGCGTTGTACCAGTTCGCTTTCCGTTACCCGACGCGCC > minE/2321308‑2321442 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |